Title page, table of contents and abstract only. The complete thesis in print form is available from the University of Adelaide Library. / Assessment of gastric emptying in animals is hampered by the lack of a technique that is noninvasive and does not involve sacrificing the animal. The primary aims of these studies therefore were to develop the breath test for the mouse and then investigate gastric emptying rates in physiological, pathological and pharmacological studies. After an overnight fast, mice were fed a solid or liquid meal that contained a ¹³C-labelled substrate, and placed in breath collection chambers. Breath samples collected at intervals were analysed for levels of ¹³C0₂, with the rate of appearance in the breath giving a measure of the gastric emptying rate. The breath testing technique was shown to be reproducible and sensitive enough to be able to detect induced alterations to gastric emptying, with results comparable to those obtained from other techniques. The breath test was used to assess gastric emptying in mice and showed that: (1) H. pylori infection accelerated gastric emptying in the initial weeks of infection, whilst a more severe stomach inflammation caused dysmotility; (2) baclofen (a potential reflux therapy) accelerated gastric emptying of solids and delayed liquid emptying in a dosedependent manner; and (3) mice with a high daily food intake had faster emptying compared to those with low intake, which resulted in the same weight gain of the two groups. Breath testing was also used in adults and children and showed that: (1) increasing the caloric content of a solid meal delayed the emptying of the solid, but not of a liquid given simultaneously; (2) gastric emptying rate is correlated to the amount of colonic gas produced; and (3) improvement in pancreatic lipase activity with pancreatic enzyme replacement is inversely related to the gastric emptying time. Breath testing was also used to show that substrates with a natural ¹³C-enrichment can be used to assess small intestinal enzyme activity. Breath testing is a sensitive and reproducible tool to investigate gastric emptying in mice. Its noninvasive nature allows it to be repeated within each subject which is useful for follow-up investigations. It can now be applied further to a range of disease, pharmacological and nutritional investigations. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1082934 / Thesis (Ph.D.) -- University of Adelaide, Dept. of Physiology, 2002

Title page, table of contents and abstract only. The complete thesis in print form is available from the University of Adelaide Library. / Assessment of gastric emptying in animals is hampered by the lack of a technique that is noninvasive and does not involve sacrificing the animal. The primary aims of these studies therefore were to develop the breath test for the mouse and then investigate gastric emptying rates in physiological, pathological and pharmacological studies. After an overnight fast, mice were fed a solid or liquid meal that contained a ¹³C-labelled substrate, and placed in breath collection chambers. Breath samples collected at intervals were analysed for levels of ¹³C0₂, with the rate of appearance in the breath giving a measure of the gastric emptying rate. The breath testing technique was shown to be reproducible and sensitive enough to be able to detect induced alterations to gastric emptying, with results comparable to those obtained from other techniques. The breath test was used to assess gastric emptying in mice and showed that: (1) H. pylori infection accelerated gastric emptying in the initial weeks of infection, whilst a more severe stomach inflammation caused dysmotility; (2) baclofen (a potential reflux therapy) accelerated gastric emptying of solids and delayed liquid emptying in a dosedependent manner; and (3) mice with a high daily food intake had faster emptying compared to those with low intake, which resulted in the same weight gain of the two groups. Breath testing was also used in adults and children and showed that: (1) increasing the caloric content of a solid meal delayed the emptying of the solid, but not of a liquid given simultaneously; (2) gastric emptying rate is correlated to the amount of colonic gas produced; and (3) improvement in pancreatic lipase activity with pancreatic enzyme replacement is inversely related to the gastric emptying time. Breath testing was also used to show that substrates with a natural ¹³C-enrichment can be used to assess small intestinal enzyme activity. Breath testing is a sensitive and reproducible tool to investigate gastric emptying in mice. Its noninvasive nature allows it to be repeated within each subject which is useful for follow-up investigations. It can now be applied further to a range of disease, pharmacological and nutritional investigations. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1082934 / Thesis (Ph.D.) -- University of Adelaide, Dept. of Physiology, 2002

Title page, table of contents and abstract only. The complete thesis in print form is available from the University of Adelaide Library. / Assessment of gastric emptying in animals is hampered by the lack of a technique that is noninvasive and does not involve sacrificing the animal. The primary aims of these studies therefore were to develop the breath test for the mouse and then investigate gastric emptying rates in physiological, pathological and pharmacological studies. After an overnight fast, mice were fed a solid or liquid meal that contained a ¹³C-labelled substrate, and placed in breath collection chambers. Breath samples collected at intervals were analysed for levels of ¹³C0₂, with the rate of appearance in the breath giving a measure of the gastric emptying rate. The breath testing technique was shown to be reproducible and sensitive enough to be able to detect induced alterations to gastric emptying, with results comparable to those obtained from other techniques. The breath test was used to assess gastric emptying in mice and showed that: (1) H. pylori infection accelerated gastric emptying in the initial weeks of infection, whilst a more severe stomach inflammation caused dysmotility; (2) baclofen (a potential reflux therapy) accelerated gastric emptying of solids and delayed liquid emptying in a dosedependent manner; and (3) mice with a high daily food intake had faster emptying compared to those with low intake, which resulted in the same weight gain of the two groups. Breath testing was also used in adults and children and showed that: (1) increasing the caloric content of a solid meal delayed the emptying of the solid, but not of a liquid given simultaneously; (2) gastric emptying rate is correlated to the amount of colonic gas produced; and (3) improvement in pancreatic lipase activity with pancreatic enzyme replacement is inversely related to the gastric emptying time. Breath testing was also used to show that substrates with a natural ¹³C-enrichment can be used to assess small intestinal enzyme activity. Breath testing is a sensitive and reproducible tool to investigate gastric emptying in mice. Its noninvasive nature allows it to be repeated within each subject which is useful for follow-up investigations. It can now be applied further to a range of disease, pharmacological and nutritional investigations. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1082934 / Thesis (Ph.D.) -- University of Adelaide, Dept. of Physiology, 2002

Thesis (M.S.)--Wisconsin. / Includes bibliographical references (leaves 42-44).

The intent of this study was to compare the rate of gastric emptying of three commercially available athletic drinks (GA, BT, and BP) against water, and in doing so to assess the exchange of water, carbohydrate and electrolytes 15 minutes after ingestion. Nine men and three women reported to the laboratory after having fasted for 12 hours.' A No. 20 French Levine Tube was inserted through the nasal passage and into the stomach. The subjects drank down 400 ml of the test solution containing 25 mg of phenol red which was used as a volume indicator. Fifteen minutes after ingestion, the gastric contents were aspirated via the tube by means of a 50 ml syringe. The 4 conditions were tested in one morning, with the order of feedings rotated between subjects. The recovered gastric contents were measured for total volume, volume of original drink, carbohydrate content and gastric electrolyte exchange. Student t values were used to test the difference between means set at the P < .05 level of confidence. BT and BP were found to empty statistically the same volume of fluid in 15 minutes as did water, while GA emptied 39% less volume than did water. BT, BP, and GA contributed 1.9, 4.5 and 6.8 gm of carbohydrate respectively in 15 minutes. These data are in agreement with previous findings that high glucose concentrations (GA - 4.5 gam) cause a slowing of gastric emptying.

The effects of limited and infrequent drinking, and atropine administration on feed intake and utilization was investigated in South African indigenous goats. Sixteen goats with an average body weight of 29.1 kg were subjected to water restriction and deprivation with concurrent atropine administration. They were fed ad libitum with a mixture of lucerne (Medicago sativa) and eragrostis hay (Eragrostis curvula), blended with molasses. The diet contained 10.47% crude protein, 38% crude fibre and 17.5 MJ/kg gross energy. Fifteen goats were randomly divided into 3 groups and were watered ad Libitum, 50% of ad libitum and 30% of ad libitum water intake respectively (Trial 1). In trial 2, a group of 8 animals were deprived of water for 3 days while the other group had free access to water daily (phase1). During phase 2, another group of 8 were watered on the 5th day while others had water ad libitum. A subgroup of 4 goats each were injected with atropine in both phases. The results showed that these goats have high water efficiency. The limited and infrequent supply of water decreased feed intake but enhanced nutrient utilisation. The provision of water at the 50% ad libitum level or once in 3 days is economical and beneficial to goat production in water-scarce areas. There is a need for complimentary investigations using atropine at high doses to further elucidate the effects of this drug on the gastro-intestinal functions of ruminants. / Dissertation (MSc (Physiology))--University of Pretoria, 2007. / Anatomy and Physiology / unrestricted