Univ., Diss., 2006--Giessen

Zugl.: Hamburg, Univ., Diss., 2002 u.d.T.: Laudahn, Björn Martin: Die indirekte Genotypanalyse als diagnostisches Verfahren beim Marfan-Syndrom / Hergestellt on demand

Thesis (doctoral)--Humboldt-University, Berlin, 2004.

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The diploma paper was focused on evaluation of chosen factors that influences loss of piglets since birth to weaning. Experiment was run in pre-chosen breeding herd on 80 sows of two genotypes A and B. The influence of genotype was evaluated for loss of piglet, then it´s influence on birth weight and sex, and for genotype A lose based on order of litter. There was statistically proven difference between genotypes in loss of piglets for litter. For genotype A the loss for litters was 1, 49 piglets and for genotype B it was 0,84 piglets for litter. There was also valid statistics prove of positive correlation between number of piglets that were born alive and number of reared piglets, however highly provable positive correlation between number of piglets born in litter and lose of piglets before weaning was established only for genotype A. When gender taken into account higher mortality of female piglet for genotype A 9,22 % than for genotype B 3,76 %. In case of male piglet the difference was not so striking 10,92 % for genotype A and 7,37 % for genotype B. The overall loss of male piglets was 5,31 % higher than for females piglets. Average postnatal weight of dead piglets did not differ for both genotypes too much (1,06 kg A, 1,02 kg B). Statistically unprovable difference was established for evaluation of postnatal weigh of dead piglets with opposite sex. During evaluation of number of piglets born alive in litter based on order of litter was established statistically provable difference between the first and the third litter and highly statistically provable difference between the second litter and the third litter. There was highly provable statistic difference in number of reared piglets between the third and the fourth litter. Highly provable statistic difference in number of dead piglets on litter between the first and the fourth litter and also between the second and the fourth litter was established. In a lose percentage was established statistically provable difference only between the second and the fourth litter.

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During recent years the possible medical application of cannabis has been discussed more and more. It turns out, that the active substances (cannabinoids cannabidiol and tetrahydrocannabinol) have a significant potential in medicine and do not have to be only misused as a recreational drug. The Department of Crop Production (Faculty of Agrobiology, Food and Natural Resources) is the first institution with granted permission to legally cultivate cannabis, with higher THC content than 0,3 %, for research purposes. The first part of literate review recapitulates the basic knowledge about the cannabis, its biologically active substances and current legislation. The second part describes the endocannabinoid system and utilization of cannabis in the field of medicine. This thesis aims to evaluate the THC and CBD content in seven different cannabis cultivar strains (Nurse Jackie, Conspiracy Kush, Jillybean, Jack Cleaner 2, Jack Skellington, Nordle and National Health Service). The cannabis plants were cultivated in three cultivation cycles. The content of active substances in dried and homogenised inflorescence was analysed by gas chromatography with flame ionization detection (GC-FID) according to UN methodology. Based on the analysis, the appropriate genotypes were chosen, which are in accordance of Regulation 236/2015 Sb. and needs of the patients. According to results it was found, that all seven analysed cultivars are suitable for medical utilization regarding the content of THC and CBD. The highest average content of THC was measured by cultivar Jack Cleaner 2. On the other hand, the lowest THC content was established by cultivar Nordle. It was demonstrated, that the differences in THC content are connected rather to genotype variation than cultivation factors. The main goal of the thesis was to discover which of the seven cultivars will be stable regarding not only THC and CBD content, but also the crop yield. In those regards the research shows the cultivar Jack Cleaner 2 and Nurse Jackie as the most suitable. Preliminary results indicate that these two cultivar strains would be the most suitable for the therapeutic purposes.

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