Thesis (Ph. D.)--Ohio State University, 2006. / Title from first page of PDF file. Includes bibliographical references (p. 118-125).

Residual feed intake (RFI) is one method to identify feed efficient animals; however, this method is costly and time consuming therefore, identifying an indirect measure of RFI is important. Evaluating the glucoregulatory mechanisms in cattle selected for divergent RFI may provide insight into metabolic processes involved in feed efficiency. This study evaluated the effect of a glucose (GLUC) challenge on efficient (LRFI) and inefficient (HRFI) tropically adapted bulls and heifers. Insulin (INS) secretion was determined by radioimmunoassay (RIA) and GLUC was determined by colorimetry. Insulinogenic index (IIND) was calculated as the ratio of INS to GLUC (I/G). Bonsmara heifers were evaluated in two experiments. Similar results were observed in both experiments. RFI affected (P < 0.05) INS response; with LRFI heifers having a greater INS response than HRFI heifers. Similarly, RFI affected (P < 0.05) IIND; with LRFI heifers having a greater IIND than HRFI heifers. In Santa Gertrudis bulls, RFI did not affect (P > 0.05) GLUC conc. or Ins. response; however, numerically HRFI bulls had a greater INS response than LRFI bulls. RFI affected (P < 0.05) IIND; with LRFI bulls having a lower IIND than HRFI bulls. In Brahman heifers (Exp 1), RFI did not affect (P > 0.05) GLUC concentration or INS. response; however, numerically HRFI heifers had a greater INS response than LRFI heifers. RFI affected (P < 0.05) IIND; with LRFI heifers having a lower IIND than HRFI heifers. In Brahman bulls (Exp 2), RFI affected (P > 0.05) INS response; with HRFI bulls having a greater INS response than LRFI bulls. RFI affected (P < 0.05) IIND; with LRFI bulls having a lower IIND than HRFI bulls. Bonsmara cattle evaluated for RFI had a response to an influx of exongenous glucose that was opposite to that observed in the Brahman and Santa Gertrudis cattle evaluated for RFI. Insulinogenic index was significantly different between RFI groups in each experiment. The lower amount of INS required for clearance of the GLUC from the circulation of the Brahman and Santa Gertrudis cattle fits with our hypothesis that more efficient cattle would require less INS than the less efficient cattle. Further research and studies need to establish glucoregulatory differences between breeds and sexes of cattle evaluated for RFI.

UDP-glucose, the precursor for cellulose biosynthesis, can be produced via the catalysis of sucrose by sucrose synthase (SuSy) or through the phosphorylation of glucose-I-phosphate by UDP-glucose pyrophosphorylase (UGPase). As such, these genes, together with sucrose phosphate synthase (SPS) which recycles fructose (an inhibitor of SuSy), are interesting targets for altering carbon allocation in plants. In an attempt to alter cell wall biosynthesis in plants, targeted overexpression of SuSy, UGPase and SPS independently and in a pyramiding strategy was assessed in tobacco. All lines displayed enhanced growth and biomass production, and in the case of double and triple transgenics, there was an additive effect. Despite the increased growth rates, there was no consistent change in soluble carbohydrate pools. Furthermore, only the triple transgenics had constant changes in structural carbohydrates: with increased hemicellulose content and slight increases in cellulose. Collectively, these results support the role of SPS, SuSy and UGPase in maintaining sink strength, but suggest that the reallocation of carbon to cellulose production in tobacco may not be possible by overexpressing these genes. In contrast, transgenic poplar overexpressing UGPase produced significantly more cellulose than wild-type trees. However, this was accompanied by a severe reduction in growth and the production of a salicylic acid glucoside (SAG) in significant quantities. The UDP-glucose generated by UGPase overexpression appeared to participate in both the synthesis of cellulose and SAG, suggesting that cellulose biosynthesis may be limited by the cellulose synthase complex. Poplar transformed with SuSy and with SuSy x UGPase also had increased cellulose production. The trees were phenotypically normal, with only minor reductions in height growth in some lines. It appears that UDP-glucose may be channelled directly to the cellulose synthase complex by SuSy. The increased cellulose content was associated with an increase in cell wall crystallinity, but there was no change in microfibril angle, confirming the re-allocation to cellulose synthesis was not the result of tension wood formation, again supporting the hypothesis that the cellulose synthase complex is the limiting factor. Clearly, it is possible to alter cellulose deposition in trees by augmenting sucrose metabolism to produce UDP-glucose, the precursor to cellulose biosynthesis.

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Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 54-60).

Thesis (Ph. D.)--University of Hong Kong, 2010. / Includes bibliographical references (leaves 168-194). Also available in print.

Thesis--M.D., University of Hong Kong, 1983.

Recent research suggests that amino acids can significantly increase skeletal muscle glucose uptake. However, the mechanism(s) have not been fully elucidated and it is also not clear if the beneficial impact amino acids have on healthy tissue translates to insulin resistant skeletal muscle. Therefore, in this series of studies, the effects of an amino acid mixture on glucose tolerance and insulin resistance were investigated. Study 1 Experiment-1 (Exp-1) demonstrated that an amino acid mixture significantly reduced the blood glucose response to an oral glucose challenge in Sprague Dawley rats. In Study 1 Exp-2, it was found that the improved glucose tolerance was due to an increase in skeletal muscle glucose uptake. The enhanced amino acid induced muscle glucose uptake was associated with improved cellular signaling. In Study 1 we could not determine the combined and/or individual effects of insulin and amino acids on glucose uptake, so in Study 2, the hindlimb of Sprague Dawley rats were perfused with glucose with or without amino acids in the presence and absence of insulin. Study 2, confirmed our previous findings that an amino acid mixture increased skeletal muscle glucose uptake compared to a carbohydrate supplement in the presence of insulin. The enhanced amino acid-stimulated glucose uptake was not due to increased phosphatidylinositol 3-kinase (PI 3-kinase) activity, although it was related to an increase in Akt substrate of 160 kDa (AS160) phosphorylation and a greater number of glucose transporters at the plasma membrane. In the final experiment, Study 3 investigated whether amino acids could improve glucose tolerance in an insulin resistant model. Study 3 Exp-1, demonstrated that an amino acid mixture significantly lowered the blood glucose response to an oral glucose challenge in obese Zucker rats. Study 3 Exp-2 showed that the improved glucose tolerance was due to enhanced amino acid induced skeletal muscle glucose uptake. Taken together, the results of this research suggests that adding an amino acid mixture to a carbohydrate supplement improves the blood glucose response to an oral glucose challenge, acutely lowers insulin resistance and this appears due to increased skeletal muscle glucose clearance and enhanced cellular signaling. / text

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