Epigeic beetle communities were studied on three agroecosystems with different crops grown. All three agroecosystems were located in the vicinity of Ceske Budejovice (Lišov). The first eco-agroecosystem was alfalfa field. On the second one was meadow. The third eco-agroecosystem was permanent grassland. The agroekosystems differd not only in crops but also in agrotechnical operations during the capture. For all agroecosystems was for capture of the organisms used method of pitfall traps. Subsequently, those organisms have been studied. Then was studied the measure of human impact on the biodiversity of species of different ecological groups found in these locations.