Electroporation is the process where externally applied electric fields cause significantly increased permeability of the cell membrane. The increased permeability allows the transport of external compounds into the cell. This is important for applications in electrochemotheropy, electrofusion and drug delivery. Electroporation also has applications in the disinfection of liquids. Given a high enough electric field across the cell membrane, the electroporation process can become irreversible, leading to cell destruction. With the cell membrane under an intense electric field, the cell membrane structure fails causing the cell to die. Conventional liquid beverage disinfection systems rely on slow heating methods requiring large power requirements; this can reduce the taste and quality of some liquids. Pulse generators provide the necessary electric fields to produce the required voltage potential across the cell membrane. The usefulness of electroporation depends on several parameters such as amplitude, frequency and rise/fall times of the electric field. The wave shape also has a bearing on performance, and is limited by the pulse generator topology. A multilevel bipolar waveform is desired with operating frequencies above about 1 kHz. The cascaded H-bridge or full-bridge topology is the most useful as it capable of producing multilevel bipolar waveforms at high frequency. This thesis presents the design and implementation of a multilevel high-voltage pulse generator, capable of creating very high-voltage AC pulses. MOSFET switching devices in conjunction with good layout practices were used to provide required fast switching speeds. The full-bridge topology is used to create a multilevel output profile through cascading of multiple stages. As a full-bridge topology inherently creates a RCL resonant network, there are many challenges associated with mitigating high-frequency noise sources. Two separate stages are built, a low voltage stage capable of outputting up to 200 Vp and a high voltage stage capable of switching up to 1 kVp. A control board was also built for pulse signal generation and user configuration of the output waveforms. The designed pulse generator can produce short pulses of up to 1.4 kVp at frequencies of up to 350 kHz using primarily resistive loads (that simulate a conductive liquid load). Little high frequency switching noise was observable on the output waveform. A single stage pulse generator was also tested with actual liquid loads using an electrode chamber, demonstrating electroporation. The liquid load testing was performed on water and milk derived from milk powder. Results showed that the liquid loads were consistent with primarily resistive loads.

The thesis ,,DC motor control by PLC Simatic S7-1200“ focuses on the control of the DC motor using the programmable automatic machine PLC Simatic S7_1200 CPU1214C, designed by the German company Siemens, generating the PWM signal. The PWM is processed by the integrated circuit called the H-Bridge, which subsequently controls the DC motor.

For this project the main goal was to design, simulate, build and test a DC motor driver. To accomplish this four specific parts had to be design. First a DCDC converter that converts the input signal from an external power supply in a controllable DC output. The second part was a DCDC converter that converts the DC input in a voltage useful for the function generator, the third part is the function generator that provides a signal to the H-Bridge, and the final part is the H-Bridge itself. The goal is to compare the measurement with the simulations to the expectations. What we are interested in is the influence of EMF when the RPM of the motor is zero or close to zero.

Segway has been a popular production as an alternative transporter since its invention at the end of 20th century. Millions of people like for its ergonomic design and high-tech elements. It is predicted to be an innovational product to change a person's life style. This thesis focuses on building a simple low cost, home-made Segway style scooter. This project uses two electric scooter motors, two 12V car batteries, one accelerometer and several microprocessors to build up the whole system. Significantly, this project also explains how to build a Brushed Direct Current (BDC) motor driver with a rated output power of more than 350W and the capability of coping with up to 120A transient peak current and up to 40A continuous current. Four-quadrant operation and eight modes of DC motor operation are discussed. A mathematical model of the Segway style scooter is also introduced in details. This including the modelling of a BDC motor, a two-wheeled inverted pendulum and their combination. The linearization of these models is used. At the end the linearized model is simulated in computer software.

The automobile industry is moving fast towards Electric Vehicles (EV); however this paradigm shift is currently making its smooth transition through the phase of Hybrid Electric Vehicles. There is an ever-growing need for integration of hybrid energy sources especially for vehicular applications. Different energy sources such as batteries, ultra-capacitors, fuel cells etc. are available. Usage of these varied energy sources alone or together in different combinations in automobiles requires advanced power electronic circuits and control methodologies. An exhaustive literature survey has been carried out to study the power electronic converter, switching modulation strategy to be employed and the particular machine to be used in an EV. Adequate amount of effort has been put into designing the vehicle specifications. Owing to stronger demand for higher performance and torque response in an EV, the Permanent Magnet Synchronous Machine has been favored over the traditional Induction Machine. The aim of this thesis is to demonstrate the use of a multi level inverter fed Brush Less Direct Current (BLDC) motor in a field oriented control fashion in an EV and make it follow a given drive cycle. The switching operation and control of a multi level inverter for specific power level and desired performance characteristics is investigated. The EV has been designed from scratch taking into consideration the various factors such as mass, coefficients of aerodynamic drag and air friction, tire radius etc. The design parameters are meant to meet the requirements of a commercial car. The various advantages of a multi level inverter fed PMSM have been demonstrated and an exhaustive performance evaluation has been done. The investigation is done by testing the designed system on a standard drive cycle, New York urban driving cycle. This highly transient driving cycle is particularly used because it provides rapidly changing acceleration and deceleration curves. Furthermore, the evaluation of the system under fault conditions is also done. It is demonstrated that the system is stable and has a ride-through capability under different fault conditions. The simulations have been carried out in MATLAB and Simulink, while some preliminary studies involving switching losses of the converter were done in PSIM.

<p>Denna högskoleavhandling beskriver konstruktionen av ett system vars funktion är att PID-reglera ett linjärställdon kopplad till venturi. Syftet har varit att få fram en reglering på venturin så att förutsättningarna förblir desamma vid mätning av olika temperaturer och lufttryck i en testrigg. Denna testrigg används för att utveckla produkten Varivent som används för att uppfylla högre miljökrav på förbränningsmotorer.</p><p>I arbetet beskriver jag de olika krav som funnits för att bygga det nya systemet, dessa var ingångar, utgångar, mikroprocessor, reglering,</p><p>kommunikation samt övriga krav. Jag diskuterar de svårigheter och möjligheter som är förknippade med kraven och tar även upp de komponenter jag använt samt deras egenskaper. Det svåraste momentet har varit PID-regulatorn som både varit en viktig och en svårlöslig del i konstruktionen av systemet. Slutligen diskuterar jag resultatet där jag, i en analys, kan se att jag kunde underlättat för mig själv med andra angreppsvinklar men att det slutliga resultatet ändå blivit mycket tillfredsställande.</p> / <p>This bachelor thesis describes the design of a unit the function of which is to regulate, using PID controls, a linear mechanical adjusting screw coupled to a variable venturi flow system. The design criteria has been to create, in a test rig, a venturi control system that gives an output that is not affected by changes in either air temperature or pressure. The test rig is used for the development of Varivent, a product used to reduce the environmental impact of the internal combustion engine. In my thesis work I describe the various and necessary elements of the project. These can be termed inputs, outputs, microprocessors, regulators and communicators. I discuss the difficulties and possibilities associated with the design criteria and mention the components used and their different attributes. The most challenging part of the project has been the design of the PID regulator, which, whilst being the heart of the system, has also been the most difficult part of the system to design. Finally I discuss my results where, in an analysis, I see that I could have made things easier for myself had I approached the problems in other ways but that the final result was, despite this, very satisfactory.</p>

Denna högskoleavhandling beskriver konstruktionen av ett system vars funktion är att PID-reglera ett linjärställdon kopplad till venturi. Syftet har varit att få fram en reglering på venturin så att förutsättningarna förblir desamma vid mätning av olika temperaturer och lufttryck i en testrigg. Denna testrigg används för att utveckla produkten Varivent som används för att uppfylla högre miljökrav på förbränningsmotorer. I arbetet beskriver jag de olika krav som funnits för att bygga det nya systemet, dessa var ingångar, utgångar, mikroprocessor, reglering, kommunikation samt övriga krav. Jag diskuterar de svårigheter och möjligheter som är förknippade med kraven och tar även upp de komponenter jag använt samt deras egenskaper. Det svåraste momentet har varit PID-regulatorn som både varit en viktig och en svårlöslig del i konstruktionen av systemet. Slutligen diskuterar jag resultatet där jag, i en analys, kan se att jag kunde underlättat för mig själv med andra angreppsvinklar men att det slutliga resultatet ändå blivit mycket tillfredsställande. / This bachelor thesis describes the design of a unit the function of which is to regulate, using PID controls, a linear mechanical adjusting screw coupled to a variable venturi flow system. The design criteria has been to create, in a test rig, a venturi control system that gives an output that is not affected by changes in either air temperature or pressure. The test rig is used for the development of Varivent, a product used to reduce the environmental impact of the internal combustion engine. In my thesis work I describe the various and necessary elements of the project. These can be termed inputs, outputs, microprocessors, regulators and communicators. I discuss the difficulties and possibilities associated with the design criteria and mention the components used and their different attributes. The most challenging part of the project has been the design of the PID regulator, which, whilst being the heart of the system, has also been the most difficult part of the system to design. Finally I discuss my results where, in an analysis, I see that I could have made things easier for myself had I approached the problems in other ways but that the final result was, despite this, very satisfactory.

Doctor of Philosophy / Department of Electrical and Computer Engineering / Behrooz Mirafzal / Corrective schemes for facilitating continued operation of dc-ac converters during internal and external abnormalities are presented in this dissertation. While some of the developed techniques are suited for any dc-ac converter topology, most of the presented methodologies are designed specifically for cascaded H-bridge (CHB) multilevel converters. While CHB provide increased scalability and efficiency compared to traditional topologies, these converters are more likely to experience internal faults due to the additional components required. Realizing the full potential of CHB converters requires fault tolerant techniques, such as those demonstrated in this dissertation. Adaptive sinusoidal pulse width modulation (ASPWM) is introduced in this dissertation as a method which enables CHB to directly utilize time-variant dc sources, increasing CHB flexibility when compared to traditional pulse width modulation (PWM) methods which require dc sources with equal magnitudes or with magnitudes existing in specific ratios. Two alternative algorithms are presented to enable ASPWM implementation, providing a trade off between system performance and required sensor circuitry. This dissertation also introduces a load independent analytical approach for identifying discordant operating points, i.e. operating points where some cells in an asymmetric CHB leg regenerate power while the overall leg delivers power, or vice-versa. Identification of these points is essential due to the deleterious effects which can result from extended discordant operation, for instance overcharging of batteries leading to lifespan degradation or even catastrophic failures such as fires or explosions. Additionally, a method for rapidly identifying, isolating, and verifying internal IGBT open-circuit and gate-driver faults is presented in this dissertation to address the increased probability of switch failures occurring within CHB. The proposed approach enables converter operation to continue in the event of gate-driver or open-circuit faults, but avoids unnecessary converter reconfiguration due to gate-misfiring faults. For a CHB leg with M cells, the proposed technique identifies and isolates open-circuit switch faults in less than 2M measurement (sampling) cycles, and verification is completed in less than one full fundamental cycle. Furthermore, this dissertation introduces a real-time implementable atypical PWM technique which enables increased dc bus utilization under a wide range of non-ideal operating conditions. While this approach is suitable for a wide range of converters operating under external abnormalities, for instance maximizing dc bus utilization for converters providing auxiliary services such as negative-sequence compensation, this approach also facilitates operation of CHB with faulty cells. The proposed method can be used with any control technique and any carrier-based PWM method, enabling its implementation in both symmetric and asymmetric CHB. In addition to these fault tolerant techniques, a novel approach for analyzing the active- and reactive-power deliverable by grid-interactive converters is proposed. This method facilitates performance comparisons for various converter configurations, simplifying the process for selecting filter components, dc bus voltages, and other system parameters. This analytical approach also enables converter performance to be analyzed during internal and external fault events, allowing assessment of converter robustness. The efficacy of the developed techniques are supported by MATLAB/Simulink simulations as well as experimental data obtained using a laboratory-scale cascaded H-bridge multilevel converter.

This paper describes the design of Stepper motor controller module controlled over Ethernet for positioning system. Part of this work describes analysis of the assignment, design of modular circuit, choosing right components and prototype design of communication and power module. In another part each module is described with emphasis to modularity. It deals with description of manufactured prototypes and their tested paramethers.

The aim of this diploma thesis is to design two different switching power supply circuits with integrated circuits suitable for controlling of LED. The theoretical part describes the principles of two main switching converters topologies – buck and boost. Furthermore, a universal H-bridge structure of switching supply is described. This is compared with the typical switching supply design. Based on specified requirements, two circuits are designed with selected IO which are suitable for using in the automotive industry. Their function and typical schema are described. In the practical part is realized design with both selected IC, choosing components and layout PCB. Set of tests are carried out with manufactured samples. Based on measured results are performed optimalization of samples, which are tested also. In the end of this thesis both samples are compared in terms of function, efficiency and EMC.