This thesis aims to investigate the relationship between increased protein dosage intake and selected haematological and biochemical markers. An experiment was designed using the Wistar laboratory rats of the male gander and similar age (born within +/- 5 days). These specimens were then divides into 3 groups with 10 specimens each. The age of the specimens at the beginning of the experiment was around 62 days. The first group was fed the standard corny mixture ad libitum, while the second group received 6 grams of the standard corny mixture and 10 grams of beef meat per day (thus reflecting the same amount of nitrous compounds as for the first group) and the last (third) group was fed ad libitum with homogenised beef meat. Before the diet was imposed on the three groups, blood samples were collected from all specimen from the lateral tail vein; after the diet was completed, blood samples were collected by in vivo heart puncture. All blood samples were subjected to haematological (using EDTA blood) and biochemical (using blood plasma) analysis. Overall, the following markers were measured: amount of erythrocytes, haematocrit level, haemoglobin level and Mean Corpuscular Volume (MCV) for the haematological analysis and triacylglycerol level, cholesterol level, amount of urea, amount of magnesium and the amount of iron for the biochemical analysis. Dietary induced significant changes were observed for the following markers: number of erythrocytes, levels of haemoglobin and MCV from the haematological analysis and cholesterol levels, amount of magnesium and amount of iron from the biochemical analysis; therefore showing that altered diet can have affect haematological and biochemical blood markers.

The topic of this diploma work was to evaluate the hematological and biochemical indicators of various breeds of dogs. The aim of this work was to evaluate the blood samples taken from 10 dogs and 10 bitches of various breeds and find out the dependency of values in relation to their age, nutrition and sex. The samples were processed and evaluated from May to October 2015. The following was examined from the hematological indicators: RBC, HGB, HCT, MCV, WBC and a blood smear was made, from which a differential count of leucocytes was made. Furthermore, the following biochemical indicators were examined: TP, GLU, CHOL and ATP. The literature research first focused on the basic components of dog feed. Moreover, the hematological and biochemical indicators of blood as well as the methodology of the whole experiment were described. The results were processed with the aid of the RStudio program and the following basic statistical characteristics were counted: median, minimum, maximum and standard deviation. The ANOVA statistical method was used, in which the dependency of values among sex, age category and the type of feed fed to the animals was determined. Furthermore, the values among the breeds were compared. From the differences observed among the breeds, there was an increase in red blood cells in German Shepherds and Dobermans. Dobermans, Labrador Retrievers and German shepherd crossbreeds had increased values of ALT. Significant findings showed that there was an increase in the number of red blood cells and hematocrits in subjects fed with granulated feed as opposed to subjects with a combined diet. The difference was also observed for MCV between the genders. Differences between the genders were found for ALT values. More data would be needed to confirm the relationship to age. ALT values are dependent on the type of feed, where the values are higher in subjects fed with a completely granulated diet. The values of total protein increased in dogs with age. This trend is likely to be observed in bitches. For subjects fed a combined diet (granulated feed + home diet) an increase in the value of total protein was observed with age. For subjects fed granulated feed, this increase was not as significant.

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Laminitis is one of the most common multifactorial diseases of horse limbs. Recent studies show that 90% of this disease is due to endocrinological diseases such as Cushing's syndrome or equine metabolic syndrome. In available literature, there is not much relevant information on the relationship of laminitis to hematological and biochemical blood parameters. This diploma thesis "Changes in selected blood parameters of horses" evaluates biochemical and haematological blood parameters in connection with laminitis in 27 sick horses. It statistically compares the differences in blood parameters between healthy and sick horses.

Thousands of parrots all over the world suffer from illnesses and medical complications that can result from interactions between their immune system and bacteria in their digestive tract. The aim of this master's thesis is to understand the link between symptoms of these medical issues, the composition of blood and gastrointestinal microbiota in parrots. Using the hematological methods, 198 blood samples representing 53 parrot species were analyzed. The composition of microbiome was defined by combination of a molecular approach using bacterial 16S rRNA gene sequencing in 132 fecal samples, 12 intestine samples, 228 cloacal swabs and 236 beak swabs representing in total 61 parrot species and a diagnostic approach by psittacine fecal Gram's stain method. Significant association of hematological parameters with individual, environmental and clinical factors was observed, as well as its considerable interspecific variability. Absolute heterophile and lymphocyte counts have been shown more useful for infectious and autoimmune disease monitoring than H/L ratio. Relative numbers of basophiles were the best indicator for behavioral disorders. In relation to hematological parameters, the effect of the bacterial family Flavobacteriaceae, as part of the oral microbiota, and the bacteria Escherichia or...

The aim of the present study was to assess an influence of two different therapeutical concentrations of peracetic acid on selected haematological and biochemical parameters in grass carp (Ctenopharyngodon idella). Fish were radomly distributed to aquaria and exposed to concentrations of 0 mg.l-1 PAA (control group), 1.0 mg.l-1 PAA (P1 group), 3.0 mg.l-1 PAA (P2 group). Almost total mortality of fish was observed in the concentration 3.0 mg.l-1 PAA during the treatment comparing with the P1 group and untreated control where no mortality was observed. After the end of the experimental exposure of fish to peracetic acid, the sampling of blood has been realised. The samples of the blood were examined in order to determine haematological and biochemical parameters. Consequently, there were no significant differences (p<0.05) in a haematological profile of fish exposed to concentration of 1.0 mg.l-1 PAA. Goblet cells count and size have risen, that caused exposure of fish to peracetic acid. In the biochemical profile of fish, significant changes (p<0.01) in three parameters were found after exposure of fish to peracetic acid in concentration 1.0 mg.l-1. Changed parameters were: aspartate aminotransferase, creatine kinase and lactate dehydrogenase. The changes were moderate and it can be supposed that these changes are reversible. No significant change (p<0.05) in haematological parameters points out to the minimum negative influence of recommended therapeutical concntration (1.0 mg.l-1 PAA) to the health of C. idella.

Diamond-Blackfan anemia (DBA) is a rare congenital syndrome that presents with ane- mia and selective deficiency of erythroid precursors, while other blood lineages are usu- ally unaffected. Approximately half of the patients display additional somatic anoma- lies and growth retardation. The therapy is mostly symptomatic and is dominated by corticosteroids, other modalities include regular blood transfusions or hematopoietic stem cell transplantation. At the beginning of this work, only two DBA causal genes were known, RPS19 and RPS24, being mutated in approximately 1/4 of all DBA patients. The goals of this work were to study the consequences of the known DBA causal mutations on cellular level and to find novel DBA causal genes. To date, over a half of DBA patients have been reported to carry a mutation in one of nine known DBA causal genes, including RPS17, RPL11 and RPL5, that are reported in this dissertation. All confirmed DBA causal genes encode for ribosomal proteins (RPs) that were essential for ribosome assembly. We further hypothesized a non- ribosomal protein participating in this process might be involved in DBA pathogenesis, too. In one DBA patient, we identified a rare sequence variant in one such candidate, a protein arginine methyltransferase 3 (PRMT3). We reported that the patient PRMT3...

Diamond-Blackfan anemia (DBA) is a rare congenital syndrome that presents with ane- mia and selective deficiency of erythroid precursors, while other blood lineages are usu- ally unaffected. Approximately half of the patients display additional somatic anoma- lies and growth retardation. The therapy is mostly symptomatic and is dominated by corticosteroids, other modalities include regular blood transfusions or hematopoietic stem cell transplantation. At the beginning of this work, only two DBA causal genes were known, RPS19 and RPS24, being mutated in approximately 1/4 of all DBA patients. The goals of this work were to study the consequences of the known DBA causal mutations on cellular level and to find novel DBA causal genes. To date, over a half of DBA patients have been reported to carry a mutation in one of nine known DBA causal genes, including RPS17, RPL11 and RPL5, that are reported in this dissertation. All confirmed DBA causal genes encode for ribosomal proteins (RPs) that were essential for ribosome assembly. We further hypothesized a non- ribosomal protein participating in this process might be involved in DBA pathogenesis, too. In one DBA patient, we identified a rare sequence variant in one such candidate, a protein arginine methyltransferase 3 (PRMT3). We reported that the patient PRMT3...

Influence of triazine based pesticide (Sencor 70 WG - active substance metribuzine) was assessed via biochemical and hematological blood profile determination of experimental fish. As experimental fish we used common carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss ). Before rating the Sencor 70 WG pesticide influence on biochemical and hematological blood profile we accomplished a preliminary test to determine a concentration range and after that a basic acute toxicity test to determine 96hLC50 value. Both fish samples fingerlings were used for performing the tests. After 96hLC50 determination two groups of experimental fish (aged 1-2 years) were under this concentration and one control group without the substance. The test was processed according to the OECD methodology No. 203. Fish dissection was performed after the test. The increased amount of phlegm of water consistency on the body surface was found out. The fish were of dark colour in contrast with the control group. The ventricle was larger and containing transudate. An outstanding vascular profusion of internal organs in the ventricle was found out. Biochemical profile of the rainbow trout (Oncorhynchus mykiss ) experimental group under 96 hour acute operation of pesticide Sencor 70 WG (89,3mg.kg-1) proved significant lowering (p<0,01) of total proteins (TP), triglycerides (TRIG), aspartate aminotransferase (AST), amonnia (NH3), alcaline phosphatase (ALP) and Ca2+ in the blood plasma in contrast with the control group. Hematological profile of the same group proved significant lowering (p<0,05) of the number of erythrocytes (Er), haematocrit (PCV), and significant increase (p<0,05) of erythrocyte haemoglobin (MCH) value in contrast with the control group. Biochemical profile of the common carp (Cyprinus carpio L.) experimental group under 96 hour acute operation of pesticide Sencor 70 WG (250,2 mg.l-1) proved significant lowering (p<0,01) of total proteins (TP), triglycerides (TRIG), lactate dehydrogenase (LDH), Ca2+ and inorganic phosphate (PHOS) and significant increase (p<0,01) of glukose (GLU) and amonnia (NH3) concentration in the blood plasma in contrast with the control group. Hematological profile of the same group proved significant lowering (p<0,01) of the haematocrit (PCV), erytrocyte haemoglobin (MCH), mean erytrocyte volume (MCV) and number of leukocytes (Leuko) in contrast with the control group.