Analysis of hemogobins were conducted on animals of the species Ambystoma tigrinum. Separation of hemoglobin types was performed with a Carboxy-methyl-cellulose column (6 X 50 mm.) and pH gradient (pH 6 thru 8) of .01 M phosphate buffer. The hemoglobins in the effluent were detected and analysed with a micro-flow cell in a Beck-man DB-G spectrophotometer attached to a Photovolt Varicord 43 recorder. Two major components of hemoglobin were observed: larval and adult. These two components varied in concentration with the stage of development of the individual. In addition, it was noted that minor peaks ansd shoulders appeared on each peak wich were consistant and characteristic for each major hemoglobin type.