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Cultivo heterotrófico de Aphanothece microscopica Nägeli e Chlorella vulgaris em diferentes fontes de carbono e em vinhaçaBonini, Monica de Albuquerque 08 March 2012 (has links)
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Previous issue date: 2012-03-08 / Financiadora de Estudos e Projetos / ABSTRACT Microalgae include both algae Chlorophycea and cyanobacteria. These organisms have attracted interest due to its versatile metabolism, since some strains have, additionaly to photosynthetic metabolism, hability to grow in the dark by the consumption of simple organic molecules, such as glucose, acetate and glycerol. This hability suggests the application of these microorganisms in several areas, such as biological treatment of agroindustrial wastewater, aiming the removal of nutrients and organic matter. São Paulo/Brazil is one of the main producers of ethanol from sugarcane, generating a considerable amount of vinasse. Vinasse represents the main wastewater from de sugarcane agroindustry, being usually applied as fertigation in sugarcane crops. In this context, this study aimed to evaluate the heterotrophic growth of cyanobacterium Aphanothece microscopica Nägeli and the chlorophyceae Chorella vulgaris in synthetic culture media supplemented with organic carbon source and in vinasse, aiming to produce biomass and application it in wastewater treatment. Mixotrophic cultures were conducted to select the optimal concentration of organic substrate for the growth of microalgae, which were evaluated in heterotrophic cultures. Additionally, experiments have been conducted with pre-treated vinasse, evaluating the COD removal and the glucose and potassium incorporation. By the mixotrophic tests was selected the optimal concentrations of 25 and 12.5 g.L-1 glucose, 0.5 and 1.25 g.L-1 potassium acetate and 0.46 and 0.92 g.L-1 glycerol as ideal for the cultivation of Aphanothece microscopica Nägeli and Chlorella vulgaris, respectively, which reflected on the maximum specific growth rate between 0.0072 and 0.043 h-1. In the heterotrophic cultures the maximum specific growth rate was greater or equal to both microalgae in all carbon sources, with reduction between 30.4 and 90% of the initial concentrations of substrates. The experiments demonstrated possibility to use vinasse as culture media for both microalgae, especially Aphanothece microscopica Nägeli. In this conditions, results indicated the maximum removals of glucose (55.5%), COD (60.8%) and potassium (13%) by the cianobacterium, and 83.7% of glucose, 25% of COD and 13.8% of potassium by Chlorella vulgaris. The results suggest the heterotrophic metabolism for both microorganisms, pointing an interesting alternative to use of wastewater as culture media to produce high-value biomass from a low-cost way. / O termo microalga inclui tanto algas clorofíceas quanto cianobactérias. Recentemente estes micro-organismos têm despertado interesse devido ao seu metabolismo versátil, uma vez que algumas linhagens apresentam, além da fotossíntese como modelo de cultivo preferencial, a capacidade de desenvolverem-se no escuro a partir do consumo de moléculas orgânicas simples, tais como glicose, acetato e glicerol. Esta habilidade sugere a aplicação destes micro-organismos em diversas áreas, como o tratamento biológico de águas residuárias agroindustriais, visando a remoção de nutrientes e matéria orgânica. Apesar disso, são raros os trabalhos na literatura que avaliam o cultivo heterotrófico e o consumo ou incorporação de moléculas orgânicas por microalgas ou cianobactérias. O Estado de São Paulo responde por grande parte da produção nacional de etanol a partir da cana-de-açúcar, gerando uma quantidade considerável de vinhaça. A vinhaça é a principal água residuária do setor sucroenergético, sendo normalmente aplicada nos canaviais como fertirrigação. Neste contexto, o objetivo deste trabalho foi estender os estudos a respeito do cultivo mixotrófico e heterotrófico da cianofícea Aphanothece microscopica Nägeli e da clorofícea Chlorella vulgaris em meios com adição de fontes de carbono orgânico e em vinhaça, visando a produção de biomassa e aplicação no tratamento de águas residuárias. Ensaios mixotróficos foram conduzidos visando selecionar a concentração ideal de substrato orgânico para o cultivo das microalgas, as quais foram posteriormente avaliadas em sistemas heterotróficos. Paralelamente foram conduzidos ensaios com vinhaça, avaliando-se a remoção de DQO e incorporação de glicose e potássio pelas microalgas. Dos ensaios mixotróficos foram selecionadas as concentrações ótimas de 25 e 12,5 g.L-1 de glicose, 0,5 e 1,25 g.L-1 de acetato de potássio e 0,46 e 0,92 g.L-1 de glicerol como ideais para o cultivo de Aphanothece e Chlorella, respectivamente, refletindo em velocidades específicas de crescimento entre 0,0072 e 0,043 h-1. Nos ensaios heterotróficos verificaram-se velocidades específicas de crescimento iguais ou superiores para ambas as microalgas em todas as fontes de carbono avaliadas, com reduções entre 30,4 e 90% da concentração inicial dos substratos. Os ensaios com vinhaça demonstraram a possibilidade de utilização desta água residuária como meio de cultivo para ambas as microalgas, com alta conversão em biomassa pela Aphanothece. Nestas condições, verificaram-se remoções de 55,5% de glicose, 60,8% de DQO e 13% de potássio para a cianobactéria, e de 83,7% de glicose, 25% de DQO e 13,8% de potássio para Chlorella. Os resultados obtidos sugerem a presença do metabolismo heterotrófico nestes microorganismos e apontam uma alternativa interessante para o aproveitamento de águas residuárias, com a possibilidade de produzir biomassa de alto valor agregado a partir de um meio de baixo custo.
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Dinâmica funcional da comunidade microbiana heterotrófica em lagoa rasa subtropicalLima, Marla Sonaira January 2011 (has links)
Ecossistemas aquáticos flutuam em torno de tendências, podendo ocorrer transições súbitas de um regime persistente para outro, alterando a viabilidade dos recursos ou dos parâmetros físico-químicos. Seguindo essa tendência, comunidades variam no tempo e no espaço como resultado de suas interações com o ambiente e com os outros organismos. Comunidades microbianas aquáticas são importantes componentes do metabolismo aquático, atuando na reciclagem de nutrientes pela remineralização e na transferência de biomassa pela alça microbiana. A compreensão da dinâmica funcional microbiana em lagos é uma importante ferramenta para o entendimento desses sistemas, uma vez que a funcionalidade e a composição microbiana podem refletir as condições gerais da lagoa em questão. Desse modo, no presente trabalho, foi utilizada a abordagem de impressões metabólicas da comunidade microbiana na Lagoa Mangueira, uma grande lagoa costeira, rasa e subtropical localizada no extremo sul do Brasil. Teve como objetivo avaliar, pioneiramente para essa região, a diversidade metabólica microbiana aquática através de padrões de consumo de fontes de carbono disponíveis, utilizando Biolog EcoplatesTM e verificar a existência de dinâmicas temporais e espaciais na preferência de consumo desses substratos. Foi observada heterogeneidade temporal e espacial na preferência de consumo de substratos ao longo da Lagoa. Tais preferências puderam ser representadas por diferentes substratos indicadores, associados às estações e aos locais da Lagoa. O consumo das fontes de carbono esteve relacionado com a variabilidade ambiental de fatores como turbidez, transparência da água, nutrientes, clorofila a, carbono orgânico temperatura. Isso evidencia que a dinâmica funcional foi influenciada pela dinâmica de nutrientes, pelos componentes de produtividade, pela sazonalidade e pela compartimentação da Lagoa. Nesse sentido, a investigação do consumo de fontes de carbono, no presente estudo, se mostrou um bom indicador da dinâmica funcional microbiana para ecossistemas aquáticos. / Aquatic ecosystems float around trends in which abrupt transitions can occur between persistents regimes, alterning the viability of the resources or the physical and chemical parameters. Following this trend, communities vary in time and space as a result of their interactions with the environment and other organisms. Microbial communities are important components in the aquatic metabolism, responsible for recycling of nutrients by remineralization, and transfering of biomass through the microbial food web. Understanding the functional microbial dynamics in lakes is an important tool to understand these systems, since the microbial composition and function may reflect the overall condition of the lake. The approach of metabolic fingerprint of microbial communities in shallow lakes was applied in the current study in Lake Mangueira, a costal and large subtropical shallow lake located in southern Brazil. The main goal was to evaluate, pioneered for this region, the heterotrophic microbial metabolic diversity through consumption patterns of available carbon sources using Biolog EcoplatesTM and verify the existence of temporal and spatial dynamics of consumption preference in these substrates. As a result, was observed temporal and spatial heterogeneity of substrate consumption preference among the Lake. Such preferences were represented by different substrate indicators, associated with seasons and sites within the Lake. The substrate utilization was related to environmental variability of factors as turbidity, water transparence, nutrients, chlorophyll a, organic carbon, water temperature. This is evidence that the functional dynamic was influenced by nutrients dynamic, production component, seasonality and compartimentation. In this way, carbon source utilization approach was a good indicator of functional dynamics in the present study to aquatic ecosystems.
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Effect of the moisture heterogeneity of leaf litter layer on temporal and spatial variation in the litter heterotrophic respiration in a warm-temperate forest / 暖温帯林の落葉層における水分の不均質性が落葉分解呼吸の時空間変動に与える影響Ataka, Mioko 23 March 2015 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19032号 / 農博第2110号 / 新制||農||1031(附属図書館) / 学位論文||H27||N4914(農学部図書室) / 31983 / 京都大学大学院農学研究科地域環境科学専攻 / (主査)教授 谷 誠, 教授 北山 兼弘, 教授 本田 与一 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM
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Spatial Heterogeneity of Ecosystem Metabolism in a Shallow WetlandRackliffe, Daniel Riley 01 December 2014 (has links) (PDF)
Spatial heterogeneity in ecosystem metabolism may play a critical role in determining ecosystem functions. Variation in ecosystem metabolism between macrophyte patches in shallow wetlands at the extremes of freshwater habitats has not been investigated. We estimated ecosystem metabolism in mesocosms containing different macrophytes using 24-hour oxygen curves to test our hypotheses: (1) net aquatic production (NAP) during spring and summer would be similar among algal patches (metaphyton and Chara), (2) NAP in algal patches would be greater than patches dominated by the vascular plant Potamogeton foliosus, (3) heterotrophy and anaerobiosis would be greatest in patches dominated by Lemna, and (4) the pond would be autotrophic in the spring and fall but heterotrophic in the summer. We found that different patches generated differences in NAP but not always as we predicted. NAP was different among algal patches in the spring and summer, and only metaphyton was more heterotrophic than P. foliosus. In the summer Chara and Lemna patches were heterotrophic and metaphyton became autotrophic. As predicted, the pond was net autotrophic in the spring and heterotrophic in the summer with an absence of patchiness in fall attributed to the dominance of Lemna. This research suggests the importance of macrophyte patchiness in wetlands in determining patterns of ecosystem metabolism despite challenges in measuring 24 hour oxygen curves (e.g. oxygen supersaturation). Consequently, macrophyte traits may be important in determining spatial heterogeneity of ecosystem metabolism in shallow ponds.
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Heterotrophic Protists as Useful Models for Studying Microbial Food Webs in a Model Soil Ecosystem and the Universality of Complex Unicellular LifeThompson, Andrew Robert 01 July 2019 (has links)
Heterotrophic protists, consisting largely of the Cercozoa, Amoebozoa, Ciliophora, Discoba and some Stramenopiles, are a poorly characterized component of life on Earth. They play an important ecological role in soil communities and provide key insights into the nature of one of life’s most enigmatic evolutionary transitions: the development of the complex unicell. Soil ecosystems are crucial to the functioning of global biogeochemical cycles (e.g. carbon and nitrogen) but are at risk of drastic change from anthropogenic climate change. Heterotrophic protists are the primary regulators of bacterial diversity in soils and as such play integral roles in biogeochemical cycling, nutrient mobilization, and trophic cascades in food webs under stress. Understanding the nature of these changes requires examining the rates, diversity, and resiliency of interactions that occur between soil organisms. However, soils are the most taxonomically diverse ecosystems on Earth and disentangling the complexities of dynamic and varied biotic interactions in them requires a unique model system. The McMurdo Dry Valleys of Antarctica, one of the harshest terrestrial environments on Earth, serve as a model soil ecosystem owing to their highly reduced biological diversity. Exploring the functioning of heterotrophic protists in these valleys provides a way to test the applicability of this model system to other soil food webs. However, very little is known about their taxonomic diversity, which is a strong predictor of function. Therefore, I reviewed the Antarctic literature to compile a checklist of all known terrestrial heterotrophic protists in Antarctica. I found significant geographical, methodological, and taxonomic biases and outlined how to address these in future research programs. I also conducted a molecular survey of whole soil communities using 18 shotgun metagenomes representing major landscape features of the McMurdo Dry Valleys. The results revealed the dominance of Cercozoa and point to an Antarctic heterotrophic protist soil community that is taxonomically diverse and reflects the structure and composition of communities at lower latitudes. To investigate whether biotic interactions or abiotic factors were a larger driver for Antarctic heterotrophic protists, I conducted variation partitioning using environmental data (e.g. moisture, pH and electrical conductivity). Biotic variables were more significant and accounted for more of the variation than environmental variables. Taken together, it is clear that heterotrophic protists play key ecological roles in this ecosystem. Deeper insights into the ecology of these organisms in the McMurdo Dry Valleys also have implications for the search for complex unicellular life in our universe. I discuss the theoretical underpinnings of searching for these forms of life outside of Earth, conclude that they are likely to occur, and postulate how future missions could practically search for complex unicells.
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Insights into Autotrophic Activities and Carbon Flow in Iron-Rich Pelagic Aggregates (Iron Snow)Li, Qianqian, Cooper, Rebecca E., Wegner, Carl-Eric, Taubert, Martin, Jehmlich, Nico, von Bergen, Martin, Küsel, Kirsten 05 May 2023 (has links)
Pelagic aggregates function as biological carbon pumps for transporting fixed organic carbon to sediments. In iron-rich (ferruginous) lakes, photoferrotrophic and chemolithoautotrophic bacteria contribute to CO2 fixation by oxidizing reduced iron, leading to the formation of iron-rich pelagic aggregates (iron snow). The significance of iron oxidizers in carbon fixation, their general role in iron snow functioning and the flow of carbon within iron snow is still unclear. Here, we combined a two-year metatranscriptome analysis of iron snow collected from an acidic lake with protein-based stable isotope probing to determine general metabolic activities and to trace 13CO2 incorporation in iron snow over time under oxic and anoxic conditions. mRNA-derived metatranscriptome of iron snow identified four key players (Leptospirillum, Ferrovum, Acidithrix, Acidiphilium) with relative abundances (59.6–85.7%) encoding ecologically relevant pathways, including carbon fixation and polysaccharide biosynthesis. No transcriptional activity for carbon fixation from archaea or eukaryotes was detected. 13CO2 incorporation studies identified active chemolithoautotroph Ferrovum under both conditions. Only 1.0–5.3% relative 13C abundances were found in heterotrophic Acidiphilium and Acidocella under oxic conditions. These data show that iron oxidizers play an important role in CO2 fixation, but the majority of fixed C will be directly transported to the sediment without feeding heterotrophs in the water column in acidic ferruginous lakes.
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UTILIZATION OF DIFFERENT FORMS OF NITROGEN BY HETEROTROPHIC BACTERIA UNDER VARYING ORGANIC CARBON CONCENTRATIONS: FROM ISOLATES TO COMMUNITIESGhosh, Suchismita 30 July 2013 (has links)
No description available.
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Mainstream Deammonification process monitoring by bacterial activity testsCarranza Muñoz, Andrea January 2020 (has links)
Deammonification is a widely used technology for side stream treatment with rich ammonium streams at relatively high temperatures, such as, the reject water coming from dewatering units in treatment of digested sludge and industrial wastewaters. The deammonification process has lower operational costs than conventional systems, consumes less energy, enables the increase of biogas production and it is easy to implement. However, this technology has not yet been applied in full- scale mainstream treatment due to its restrictions in coping with high C/N ratios, low temperatures, and the need for post-treatment processes. These conditions are allegedly negative to the growth and performance of anammox bacteria affecting the bacterial groups’ behavior in the process. This master thesis project aimed to evaluate the feasibility of using deammonification to remove nitrogen from mainstream wastewater, which was studied by monitoring the bacterial activity in a pilot scale reactor. The different bacterial groups involved (AOB, NOB, heterotrophs, and denitrifiers) were monitored by weekly measuring their activity in batch activity tests. The results allowed the evaluation of different operational scenarios and their impact by following up on the changes in the bacterial competition. The study was conducted for six months in a single-stage IFAS (integrated fixed-film activated sludge) pilot-scale reactor located in Stockholm and fed with pretreated (with a UASB) municipal wastewater. The different operational scenarios involved changes in temperature, aeration patterns, DO concentration, SRT, and HRT. The adjustment of these features was done in the interest of promoting AOB and anammox bacterial growth, leading to an improvement of the deammonification efficiency in future studies. However, the chosen operational conditions were to enhance bacterial competition and facilitate its visualization, not to maximize nitrogen removal. Thus, the most suitable scenario found during this study included DO concentration of 1.5 mg/L with 10 aeration-20 non-aeration pattern and ensured nitrogen removal rates within normal values while allowing the monitoring of all the bacterial groups. TN removal reached a value above 50% and NH4-N above 95%, whereas nitrogen Removal Rate (NRR) increased to 30g/N/m3-d and the system had an overall nitrogen removal efficiency of 75%. Nevertheless, it was proven that in the right environment, the necessary bacterial groups can be selectively accumulated and successfully perform deammonification and reduce nitrogen levels in mainstream wastewater. / Deammonifikation är en välanvänd teknik för rening av sidoströmmar med höga ammoniumkoncentrationer vid relativt hög temperatur, som till exempel rejektvatten från avvattning av rötslam eller industriellt avloppsvatten. Deammonifikationsprocessen har lägre driftkostnad än konventionella reningsprocesser, förbrukar mindre energi samt möjliggör högre biogasproduktion samtidigt som processen är enkel att implementera. Reningstekniken har dock ännu inte tillämpats i fullskala för rening av huvudströmmen på grund av den höga C/N-kvoten och de låga vattentemperaturerna i kommunalt avloppsvatten samt behovet av efterbehandling. Detta anses ha en negativ inverkan på anammoxbakteriernas tillväxthastighet och funktion vilket påverkar bakteriegruppens beteende i processen. Syftet med detta examensarbete var att utvärdera om det är praktiskt genomförbart att använda deammonifikation för att rena kväve från kommunalt avloppsvatten, vilket följdes upp genom att studera bakterieaktiviteten i en pilotskalereaktor. De involverade bakteriegrupperna (AOB, NOB, heterotrofer och denitrifierare) övervakades genom att mäta den mikrobiella aktiviteten varje vecka med hjälp av batch-tester. Resultaten användes till att utvärdera olika driftstrategier och deras effekt genom att följa förändringarna i mikrobiell aktivitet hos de konkurrerande bakteriegrupperna. Studien genomfördes i Stockholm under sex månader i en enstegs-IFAS-pilotskalereaktor (integrerad process med biofilm på fast bärarmaterial och aktivslam) som matades med kommunalt avloppsvatten som förbehandlats i en UASB-reaktor. De olika driftstrategierna omfattade olika temperaturer, luftningsstrategier, syrekoncentrationer, slamåldrar och hydrauliska uppehållstider. Syftet med driftstrategierna var att främja AOB- och anammoxbakteriers tillväxt för att i framtida studier kunna erhålla en förbättrad deammonifikationsprocess. Syftet i denna studie var dock i första hand att förbättra den bakteriella konkurrensen och göra den lättare att mäta, inte att uppnå bästa möjliga kväverening. Den driftstrategi som gav bäst resultat i denna studie innebar att hålla en syrehalt på 1,5 mg/l med 10 minuter luftning följt av 20 minuter utan luftning vilket säkerställde en normal kväveavskiljning och samtidigt möjliggjorde övervakning av samtliga fyra bakteriegrupper. Totalkväveavskiljningen var över 50 % och ammoniumavskiljningen över 95 % medan kvävereningsaktiviteten ökade till 30 g N/m3-d och systemet hade en övergripande effektivitet på 75 %. Studien visade att under rätt förutsättningar kan de nödvändiga bakteriegrupperna selekteras fram och deammonifikation av kommunalt avloppsvatten kan utföras på ett framgångsrikt sätt.
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Phytoplankton Ecology of Lake Kivu (Eastern Africa)/ Écologie du phytoplancton du lac Kivu (Afrique de l'Est)Sarmento, Hugo 29 September 2006 (has links)
With a volcanic origin, Lake Kivu is deep and meromictic, and shows a very particular limnology and some astonishing features. The data available on its limnology and phytoplanktic communities are limited, dispersed or outdated. This is the first deep, long term study (2002-2004) on limnology and phytoplankton ecology of Lake Kivu, combining different techniques: HPLC analysis of marker pigments, flow cytometry, light, epifluorescence and electron microscopy. Lake Kivu combines a relatively shallow euphotic layer (~18m) usually smaller than its mixed layer (20 – 60 m), and with a weak thermal gradient in the mixolimnion. With an annual average chlorophyll a in the mixed layer of 2.2 mg m-3 and low nutrient levels in the euphotic zone, the lake is clearly oligotrophic. Concerning its phytoplanktonic composition, the most common species were the pennate diatoms Nitzschia bacata Hust. and Fragilaria danica (Kütz.) Lange Bert., and the cyanobacteria Planktolyngbya limnetica (Lemm.) Komárková-Legnerová and Cronberg and Synechococcus sp. Diatoms were the dominant group in the lake, particularly during the dry season episodes of deep mixing. During the rainy season, the stratified water column, with high light and lower nutrient availability, favoured dominance of cyanobacteria. Phycoerythrin-rich phototrophic picoplankton and heterotrophic bacteria cell numbers were constantly high, with relatively subtle spatial, seasonal and vertical variations. In open lake waters, where allochthonous carbon inputs are most probably inconsequential, HNA heterotrophic bacteria abundance is strongly correlated with chlorophyll a. Recent investigations revealed an increasing methane production in the deep waters during the past three decades, leading to an accumulation of gas and the subsequent lowering of the energy required to trigger a devastating release of gasses. The role of primary producers and the probable changes on the export ratio of the organic matter into deep waters after the introduction of the planktivorous sardine from Lake Tanganyika Limnothrissa miodon, is discussed. The actual primary production, 0.71 g C m-2 d-1 (~ 260 g C m-2 y-1), doesn’t seem substantially different from some punctual measurements made in the past, which discards the hypothesis of anthropogenic eutrophication. We believe that the export ratio of organic matter into the deep waters was largely affected by biological changes that could explain part of the methane increase in the past 30 years in Lake Kivu.
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Treatment of mature urban landfill leachates by anammox processRuscalleda Beylier, Maël 17 February 2012 (has links)
This thesis results from the collaborative projects between the LEQUIA-UdG group and Cespa (a company in charge of several landfill sites in Spain). The aim of the work was the development of a suitable alternative treatment for nitrogen removal from mature landfill leachates. The thesis presents the application of the anammox (anaerobic ammonium oxidation process) process to treat ammonium rich leachates as the second step of the PANAMMOX® process. The work deals with preliminary studies about the characteristics of the anammox process in a SBR, with special focus on the response of the biomass to nitrite exposure. The application of the anammox process with leachate was first studied in a lab-scale reactor, to test the effect of the leachate matrix on anammox biomass and its progressive adaptation. Finally, a start-up strategy is developed and applied for the successful start-up of a 400L anammox SBR in less than 6 months. / Aquesta tesi és fruit de la col•laboració entre el grup LEQUIA-UdG i Cespa. L'objectiu del treball va ser el desenvolupament d'un tractament alternatiu per a l'eliminació biològica de nitrogen dels lixiviats madurs d'abocador. La tesi presenta l'aplicació del procés anammox (anaerobic ammonium oxidation) per tractar elevades càrregues de nitrogen en el segon pas del procés PANAMMOX ®. El treball inclou estudis preliminars sobre les característiques del procés de anammox en un SBR, amb especial atenció a la resposta de la biomassa a l'exposició de nitrit. L'aplicació del procés anammox amb lixiviat es va estudiar inicialment en un reactor a escala de laboratori, per provar l'efecte de la matriu del lixiviat sobre la biomassa anammox i la seva adaptació progressiva. Finalment, es va desenvolupar una estratègia de posada en marxa que va ser aplicada amb èxit per a la posada en marxa d'un SBR anammox de 400L en menys de 6 mesos.
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