Manduca sexta vitellogenin is a phosphoglycolipoprotein (Mᵣ ∼ 500,000) that contains two copies of the apoproteins (apovitellogenin-I, Mᵣ 180,000 and apovitellogenin-II Mᵣ 45,000), 13 percent lipids, 3 percent carbohydrates and 0.6 percent phosphorus. The two apoproteins are immunologically distinct and apovitellogenin-II is not completely accessible to the aqueous environment in the intact molecule. The carbohydrate moiety located on apovitellogenin-I has a high mannose structure (Man₉ GlcNAc₂). Follicle membranes bind ¹²⁵I-labeled vitellogenin with high affinity and specificity (K(D) ≃ 1.3 x 10⁻⁸ M). Total binding sites were estimated at 4 x 10¹⁴ sites/g of follicle membrane protein. The binding was sensitive to pH and calcium. Competition studies showed that binding of vitellogenin was blocked by vitellin and deglycosylated vitellogenin but not by lipophorin, microvitellogenin or apovitellogenin-II. These results suggest that the uptake of vitellogenin involves binding to specific receptors on follicle membranes and the carbohydrate moiety and apovitellogenin-II are not involved in the interaction with the receptors.

No description available.

Thesis (doctoral)--Swedish University of Agricultural Sciences, 2003. / Appendix consists of reprints and manuscripts of five papers co-authored with others. Includes bibliographical references. Also partially available electronically via World Wide Web in PDF format; online version lacks appendix.

Eight-hundred and forty-eight tobacco (Protoparce sexta (Johan.)) and tomato (P. quinquemaculata (Haw.)) hornworm moths were irradiated individually with energy bands, nominally 33 Angstroms wide, centered at 3129, 3341, 3654, 4047, 4358, 4916, 5461, and 5780 A. Tests were made in a light-tight, air conditioned chamber. An analysis of variance was used to determine differences in responses to treatments. A significant difference in response to waveband treatments was found in each moth group. Generally, responses to the four shorter wavelengths were greater than to the four longer. When considering all moths or all trapped moths, best responses were to 3129 and 3341 Angstroms and second best were to 3654 and 4047 Angstroms. Reared moths were more responsive to treatments than trapped moths. Greatest response was obtained from the reared male tobacco hornworm moths. Tomato hornworm moths were less responsive to treatments than tobacco hornworm moths. The female tomato hornworm moths 1rJere numerically, least responsive. Numerically, more responses were elicited from first exposures than from those received later. / Master of Science

In this investigation a laboratory apparatus was designed and constructed suitable for testing various commercially avai1able electric lamps as attractors moths for moths of both tobacco hornworm (Protonaree sexta (Johan.)) and tomato hornworm (Protoparce quinquemaculata (Haw.)) species. / Master of Science

Eight-hundred-three moths were irradiated with 10-second exposures of radiation in bands 100 Angstroms wide at 200 A. intervals. The main range covered was 3150 to 4550 A. Four controlled temperatures between 60 and 80 F. were used. Observations of individual moth responses were combined to determine the most stimulating band, the effect of temperature, and effects of species, sex, time of testing, and moth origin. Within the wavelength range, a band centered at 3150 A. had the most reactions and one at 4350 A. the least. The total group of moths tested over these bands had a plot of reactions versus wavelength with no reversals of slope between maximum and minimum. No definite effects of temperature, sex, time of testing, or moth origin were found. The tomato hornworm moth was definitely the less active species and showed a greater difference between bands of maximum and minimum reactions. An additional test found no effect due to the relative intensity of radiation on different bands, which was not equalized in the main tests. Only teat groups which allowed for several hundred possible moth reactions at each treatment showed consistent trends. / Master of Science

Fat body tissue was taken from female Manduca larvae throughout the fifth instar. The samples were prepared for histological study and electron microscopy. Hemolymph samples were collected and analyzed for protein profile and concentration. The data showed that the fat body underwent a series of changes with development. These include an increase in cell size, and accumulation of lipid and glycogen during the feeding phase of the instar, and the formation of protein and urate granules during the wandering stage. Also apparent was the cyclic development of a reticular system on the cell surfaces. Maximum development of the system coincided with the period of highest protein concentration in the hemolymph, while its disappearance is coincident with a drop in hemolymph protein concentration and formation of fat body granules. Thus the fat body plays a synthetic role early in the instar and becomes a storage tissue as pupation approaches.

Thesis (Ph. D.)--University of Oregon, 2005. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 118-133). Also available for download via the World Wide Web; free to University of Oregon users.

Thesis (Ph.D.)--Tufts University, 2003. / Adviser: Barry A. Trimmer. Submitted to the Dept. of Biology. Includes bibliographical references (leaves 165-178). Access restricted to members of the Tufts University community. Also available via the World Wide Web;

Thesis (M.S.)--University of Nevada, Reno, 2006. / "August, 2006." Includes bibliographical references (leaves 112-116). Library also has microfilm. Ann Arbor, Mich. : ProQuest Information and Learning Company, [200x]. 1 microfilm reel ; 35 mm. Online version available on the World Wide Web.