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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

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Showing 1 to 2 of 2 (0.116 seconds)
1

Tamoxifen-Independent Recombination in the RIP-CreER Mouse

Solimena, Michele, Steffen, Anja, Magro, Maria Grazia, Masjkur, Jimmy, Suckale, Jackob, Liu, Yanmei, Anastassiadis, Konstantinos 02 December 2015 (has links) (PDF)
Background The inducible Cre-lox system is a valuable tool to study gene function in a spatial and time restricted fashion in mouse models. This strategy relies on the limited background activity of the modified Cre recombinase (CreER) in the absence of its inducer, the competitive estrogen receptor ligand, tamoxifen. The RIP-CreER mouse (Tg (Ins2-cre/Esr1) 1Dam) is among the few available β-cell specific CreER mouse lines and thus it has been often used to manipulate gene expression in the insulin-producing cells of the endocrine pancreas. Principal Findings Here, we report the detection of tamoxifen-independent Cre activity as early as 2 months of age in RIP-CreER mice crossed with three distinct reporter strains. Significance Evidence of Cre-mediated recombination of floxed alleles even in the absence of tamoxifen administration should warrant cautious use of this mouse for the study of pancreatic β-cells.
Insulin
Methoden Immunhistochemie
Östrogene
Expremierung
Mouse models
Insulin
Immunohistochemistry techniques
Estrogens
gene expression
staining
genetic loci
recombinant proteins
ddc:181
rvk:XA 10000
2

Tamoxifen-Independent Recombination in the RIP-CreER Mouse

Solimena, Michele, Steffen, Anja, Magro, Maria Grazia, Masjkur, Jimmy, Suckale, Jackob, Liu, Yanmei, Anastassiadis, Konstantinos 02 December 2015 (has links)
Background The inducible Cre-lox system is a valuable tool to study gene function in a spatial and time restricted fashion in mouse models. This strategy relies on the limited background activity of the modified Cre recombinase (CreER) in the absence of its inducer, the competitive estrogen receptor ligand, tamoxifen. The RIP-CreER mouse (Tg (Ins2-cre/Esr1) 1Dam) is among the few available β-cell specific CreER mouse lines and thus it has been often used to manipulate gene expression in the insulin-producing cells of the endocrine pancreas. Principal Findings Here, we report the detection of tamoxifen-independent Cre activity as early as 2 months of age in RIP-CreER mice crossed with three distinct reporter strains. Significance Evidence of Cre-mediated recombination of floxed alleles even in the absence of tamoxifen administration should warrant cautious use of this mouse for the study of pancreatic β-cells.
info:eu-repo/classification/ddc/181
ddc:181

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