Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2018-09-17T22:06:41Z No. of bitstreams: 1 Disserta??o de Mestrado - Washington.pdf: 1852656 bytes, checksum: 454b1394baec4b0dbe61bc22fcf2e3ee (MD5) / Made available in DSpace on 2018-09-17T22:06:41Z (GMT). No. of bitstreams: 1 Disserta??o de Mestrado - Washington.pdf: 1852656 bytes, checksum: 454b1394baec4b0dbe61bc22fcf2e3ee (MD5) Previous issue date: 2018-02-06 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The fungi Lasiodiplodia theobromae and Neofusicoccum parvum are pathogens responsible for reducing mango yield due to dieback, which both cause in the culture. Inheritance studies and variability in accessions for fungal diseases in mango, in general, are quite limited, despite their importance for the development of cultivars. The goals of this study were1) to evaluate the inheritance of resistance to dieback disease caused by L. theobromae and N. parvumin three crossings, and 2) evaluate 75mango accessions for resistance to these fungi to support mango breeding programs. Young branches containing young leaves were selected, and suspension of 103 conidia/mL of L. theobromae or N. parvum was sprayed, separately. After inoculation, leaves were protected in a plastic wet chamber for 48 hours. The infection was estimated by taking the branches without symptoms/total number of inoculated branches. Plants without disease symptoms in more than 90% of the brancheswere considered as resistant. Monogenic resistance, conferred by recessive alleles, was observed for L. theobromae for the three crossings evaluated, despite the 13:3 segregation detected for 'Keitt' x 'Tommy Atkins'. For N. parvum, recessive resistance with 3:1 monogenic segregation was observed in the crossing 'Keitt' x 'Tommy Atkins', and 9:7 epistatic segregation was observed in the crossing ?Haden? x ?Tommy Atkins? and ?Espada x ?Tommy Atkins?. ?Apple DCG?, ?Ataulfo?, ?Da porta?, ?Dashehari?, ?Espada?, ?Haden?, ?Haden 2H?, ?Heidi?, ?Irwin?, ?Keitt?, ?Kent?, ?N?ldica?, ?Recife? and ?Smith? were resistant to both pathogens, being promising for breeding programs. 'Rosa' are resistant to L. theobromae, and susceptible to N. parvum. 'Tommy Atkins', the most grown cultivar in the S?o Francisco valley, was susceptible to both pathogens.The mono or digenic inheritance observed in the present study indicates the possibility of selection in the F2 generation and identification of plants with double resistance to L. theobromae and N. parvum in mango trees. / Os fungos Lasiodiplodia theobromae e Neofusicoccum parvum s?o pat?genos que podem limitar a produ??o da mangueira devido a doen?a morte-descendente, que ambos ocasionam na cultura. Estudos de heran?a da resist?ncia e da variabilidade em acessos para doen?as f?ngicasem mangueira, em geral, s?o bastante limitados, apesar da sua import?ncia para o desenvolvimento de cultivares. Este estudo teve como objetivos 1) avaliar a heran?a da resist?ncia ? doen?a morte-descendente causadas por L. theobromae e N. parvumem tr?s cruzamentos, e 2) avaliar 75acessos de mangueira para resist?ncia aos dois fungos, para apoiar programas de melhoramento da cultura. Foram selecionados ramos jovens contendo folhas jovens para aplica??o, com aux?lio de pulverizador manual, de suspens?o de con?dios de 103 con?dios/mL de L. theobromae ou N. parvum. Ap?s a inocula??o, as folhas foram protegidas em c?mara ?mida de pl?stico durante 48 horas. A infec??o foi estimada tomando-se ramos sem sintomas / n?mero total de ramos inoculados. As plantas sem sintomas de doen?a em mais de 90% dos ramos, em diferentes per?odos de inocula??o, foram consideradas resistentes.Foi observada resist?ncia monog?nica conferida por alelos recessivos para L. theobromae, para os tr?s cruzamentos avaliados, apesar da segrega??o 13:3 para ?Keitt? x ?Tommy Atkins?. Para N. parvum foi observada resist?ncia recessiva com segrega??o monog?nica 3:1 no cruzamento ?Keitt? x ?Tommy Atkins? e segrega??o epist?tica 9:7 nos cruzamentos ?Haden? x ?Tommy Atkins? e ?Espada x ?Tommy Atkins?. Os acessos ?Apple DCG?, ?Ataulfo?, ?Da porta?, ?Dashehari?, ?Espada?, ?Haden?, ?Haden 2H?, ?Heidi?, ?Irwin?, ?Keitt?, ?Kent?, ?N?ldica?, ?Recife? e ?Smith?apresentaram resist?ncia aos dois pat?genos, sendo bastante promissoras para programas de melhoramento. ?Rosa? apresentou resist?ncia a L. theobromae, sendo considerada suscet?vel ao N. parvum. ?Tommy Atkins?, a mais cultivada no vale do S?o Francisco, foi suscept?vel a ambos os pat?genos. A heran?a mono ou dig?nica observada no presente estudo indica a possibilidade de sele??o na gera??o imediata aos cruzamentos, bem como a possibilidade da identifica??o de plantas com resist?ncia dupla a L. theobromae e N. parvum em mangueira.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-08T16:51:22Z No. of bitstreams: 1 2016 - Rennan do Amaral Bastos.pdf: 2824478 bytes, checksum: 5a974a7444eaef272210a147add547a0 (MD5) / Made available in DSpace on 2017-02-08T16:51:22Z (GMT). No. of bitstreams: 1 2016 - Rennan do Amaral Bastos.pdf: 2824478 bytes, checksum: 5a974a7444eaef272210a147add547a0 (MD5) Previous issue date: 2016-02-04 / The objective of this work was to evaluate the production of biomass and grains in common bean (Phaseolus vulgaris) in co-inoculation with rhizobia and plant growth promoting bacteria. The work was divided into three chapters. The first evaluated the compatibility between rhizobia and plant growth promoting bacteria and their effects on plant growth. In Petri dishes assay, Azospirillum amazonense and A. brasilense were co-inoculated with Rhizobium tropici at three application levels, and no antagonism was detected. The experiment in Leonard jars had a 5x3+3 factorial with eight replications, consisting of five bacteria (Bradyrhizobium diazoefficiens, B. elkanii, A. amazonense, Azospirillum brasilense and Escherichia coli) and three application levels (104, 106 and 108 CFU mL-1), all co-inoculated with R. tropici plus three additional treatments (control, nitrogen, and inoculated with R. tropici). Co-inoculation increased nodulation, N concentration and content in the shoot. The experiment in pots with soil had 3x2x2+4 factorial with four replications, consisting of three growth promoters (B. diazoefficiens, B. elkanii and A. brasilense), two application levels (104 and 106 CFU mL-1), two N sources (R. tropici and mineral N), and four additional control treatments (absolute control, nitrogen, inoculated with rhizobia and co-inoculated with AZOTOTAL?). Inoculation with A. brasilense associated with N fertilization increased the production of leaves and shoots. The second chapter aimed to evaluate the growth and production of common bean under co-inoculation. An experiment in pots with soil in a factorial 2x3+4 with 8 repetitions were performed by combining the inoculation of R. tropici with A. brasilense or B. diazoefficiens in three application levels (104, 106 and 108 CFU mL-1) plus the control treatments (absolute, nitrogen, inoculated with rhizobia and co-inoculated with AZOTOTAL?). Four replications were harvested at 45 days after sowing, and four at grain maturity. Co-inoculated plants showed shoot mass, number of pods, shoot N content, grain yield, number of seeds and pods per plant higher than the control inoculated with rhizobia. The leaf chlorophyll Falker index was similar in co-inoculated treatments and under mineral N fertilization, but lower than the exclusive inoculation R. tropici. In the third chapter it was evaluated the growth and production of common bean under different strategies of mineral N fertilization associated with co-inoculation with R. tropici and A. brasilense. The experiment had seven treatments with four replications: control; inoculation with R. tropici without N application; control with mineral N; co-inoculation with R. tropici and A. brasilense without N fertilization or application of 20 kg ha-1 of N at sowing; inoculation with R. tropici and 20 kg ha-1 of N at sowing; inoculation with A. brasilense and 20 kg ha-1 of N at sowing. Plants were harvested at 45 days after sowing and at grain maturity. Co-inoculation combined with the N fertilization resulted in higher mass values of leaves, stem, shoot, dry mass of grains, number of grains per plant and accumulation of N in pods and grains. / O objetivo do trabalho foi avaliar a produ??o de biomassa e de gr?os em feijoeiro comum (Phaseolus vulgaris) sob co-inocula??o de riz?bio e bact?rias promotoras de crescimento vegetal. O trabalho foi dividido em tr?s cap?tulos. O primeiro teve como objetivo avaliar a compatibilidade entre riz?bio e bact?rias promotoras de crescimento e seus efeitos no crescimento das plantas. Em ensaio em placas de Petri, Azospirillum amazonense e A. brasilense foram co-inoculados com Rhizobium tropici em tr?s doses de aplica??o, verificando-se aus?ncia de antagonismo. O experimento em vasos de Leonard teve esquema fatorial 5x3+3 com oito repeti??es, composto por cinco bact?rias (Bradyrhizobium diazoefficiens, B. elkanii, Azospirillum amazonense, A. brasilense e Escherichia coli) e tr?s doses de aplica??o (104, 106 e 108 UFC mL-1), todas co-inoculadas com R. tropici, e tr?s tratamentos adicionais (testemunhas absoluta, nitrogenada, e inoculada com R. tropici). A co-inocula??o aumentou a nodula??o, teor e conte?do de N na parte a?rea. O experimento em vasos com solo teve esquema fatorial 3x2x2+4 com quatro repeti??es, composto por tr?s promotores de crescimento (B. diazoefficiens, B. elkanii e A. brasilense), duas doses (104 e 106 UFC mL-1), duas fontes de N (R. tropici e N mineral), e quatro tratamentos adicionais (testemunhas absoluta, nitrogenada, inoculada com riz?bio e co-inoculada com AZOTOTAL?). A inocula??o com A. brasilense associada ? aduba??o nitrogenada aumentou a produ??o de folhas e de parte a?rea. O segundo cap?tulo teve como objetivo avaliar o crescimento e produ??o do feijoeiro sob co-inocula??o. Foi realizado um experimento em vasos com solo em esquema fatorial 2x3+4 com oito repeti??es, combinando a inocula??o de R. tropici com A. brasilense ou B. diazoefficiens e tr?s doses de aplica??o (104, 106 e 108 UFC mL-1), acrescidos das testemunhas absoluta, nitrogenada, inoculada com riz?bio e co-inoculada com AZOTOTAL?. As plantas foram coletadas aos 45 dias ap?s plantio e na matura??o dos gr?os. As plantas co-inoculadas apresentaram massa de parte a?rea, n?mero de vagens, conte?do de N na parte a?rea, massa de gr?os, n?mero de gr?os e de vagens por planta, superiores ? testemunha inoculada com riz?bio. O ?ndice de clorofila Falker foi similar nos tratamentos co-inoculados e sob aduba??o nitrogenada, mas inferior ao da inocula??o com R. tropici. No terceiro cap?tulo avaliou-se o crescimento e produ??o do feijoeiro sob diferentes estrat?gias de aduba??o nitrogenada associada ? co-inocula??o. O experimento consisitu de sete tratamentos com oito repeti??es: testemunha absoluta; inocula??o com R. tropici sem aplica??o de N; testemunha com N mineral; co-inocula??o com R. tropici e A. brasilense sem N ou com 20 kg ha-1 de N em semeadura; inocula??o com R. tropici e 20 kg ha-1 de N em semeadura; inocula??o com A. brasilense e 20 kg ha-1 de N em semeadura. As plantas foram coletadas aos 45 dias e na matura??o. A co-inocula??o combinada com a aduba??o nitrogenada resultou em maiores valores de massa de folhas, caule, parte a?rea, massa seca de gr?os, n?mero de gr?os por planta e ac?mulo de N nas vagens e gr?os.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-03-23T14:47:27Z No. of bitstreams: 1 2016 - Jo?o Paulo de Lima Aguilar.pdf: 2057822 bytes, checksum: e5eac0160e0ba66e10d0af7b9cf5437f (MD5) / Made available in DSpace on 2017-03-23T14:47:27Z (GMT). No. of bitstreams: 1 2016 - Jo?o Paulo de Lima Aguilar.pdf: 2057822 bytes, checksum: e5eac0160e0ba66e10d0af7b9cf5437f (MD5) Previous issue date: 2016-02-29 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / This work was carried out in Area Horticulture, Crop Science Department of Agronomy Institute of the Universidade Federal Rural do Rio de Janeiro. It was divided in to two stages, on initially in germination greenhouses and the second stage was performed in a protected environment. Statice seeds were inoculated with freeze-dried peat inoculant Azospirillum Sp245 (2,8x109 ufc g-1) as treatment concentration (Az), and compared to the control seeds, with out Azospirillum. In the first step, were seeded 18 polypropylene trays with 50 cells with a volume of 17ml each, with the substrate BIOMIX? seedlings. The following parameters were analyzed: percentage of germination (%Germ), root volume (RV), total length (CTT.), length of aerial parts (CPA), total of fresh material (MFT), total of dry material (MST), and length of roots (CR.).This step was planned in a completely randomized design. In the second stage, seeds were inoculated and sown in a protected environment, at the same time of the beginning of the first stage. They were divided into two plots of 20 m long with six blocks in each bed. Each treatment contained 21 plants. In the interval of 21 days, the plants were fertilized with ?torta de mamona? and ?Bokashi?, alternately with an average of 15 days between the applications. The parameters selected for evaluation were as follows: number of leaves(NF), root length (CR), shoot length (CPA), fresh matter of roots(MFR.), dry matter of root(MSR), root length(CR), root volume (RV), fresh matter of the aerial parts (MFPA), dry matter or aerial parts (MSPA), and rate between aerial part and roots(MSPA/MSR). The experiment began on August 15th2015andfinishedon November 28th2015. The first step lasted 42 days and the second step 63days, in a total of 105days. In the first step, the use of Azospirillum has influenced on all parameters. In the second step, the parameters: number of leaves, shoot/root ratio, fresh weigh to roots, dry wheight and root, and root volume were positively influenced by the treatment. We can cite three possible causes to explain why the other analyzes were not significant. The lack of water in certain periods; high temperature or heat stroke in plant closure period may have aborted the flower stalks; and competition with existing weeds in place, especially Cyperaceaae. The use Azospirillum Sp245 as inoculants proved to be avaliable alternative in the cultivation of statice (Limonium sinuatum) as a cut flower / Este trabalho foi realizado na ?rea de Horticultura, do departamento de Fitotecnia do Instituto de Agronomia da Universidade Federal Rural do Rio de Janeiro. Foi dividido em duas etapas, uma inicial em estufas de germina??o e a segunda em ambiente protegido. Sementes de statice foram inoculadas com o inoculante turfoso contendo Azospirillum Sp245 (2,8x109 ufc g-1) como tratamento (Az) e comparadas com sementes n?o tratadas (inoculadas). Na primeira etapa de avali??o, foram semeadas 18 bandejas de polipropileno, com 50 c?lulas com volume de 17 ml, preenchidas com o substrato BIOMIX? mudas, sendo analisados os seguintes par?metros: percentual de germina??o (%Germ), volume de raiz (VR), comprimento total (CTT), comprimento da parte a?rea (CPA), mat?ria fresca total (MFT), mat?ria seca total (MST) e comprimento da raiz (CR). O delineamento experimental foi inteiramente casualizado. Na segunda etapa, sementes que foram inoculadas e semeadas na mesma ?poca do in?cio da primeira etapa, foram plantadas numa ?rea em ambiente protegido, divididos em dois canteiros com 20 m de comprimento e com seis blocos em cada canteiro. Cada tratamento continha 21 plantas. No intervalo de 21 dias, as plantas foram fertilizadas com torta de mamona e bokashi, alternadamente com uma m?dia de 15 dias entre as aplica??es. Os par?metros que foram selecionados para serem avaliados foram os seguintes:n?mero de folhas (NF), rela??o entre a parte a?rea e as ra?zes (RPA/RZ), comprimento da raiz (CR), comprimento parte a?rea (CPA), massa fresca da raiz (MFR), massa seca da raiz (MSR), comprimento da raiz (CR), volume da raiz (VR), massa fresca da parte a?rea (MFPA), massa seca da parte a?rea (MSPA), in?cio da produ??o das flores (IF), peso das hastes florais (PHF), n?mero de espiguetas (NE) e n?mero de flores por espigueta (NFE). O experimento teve in?cioem 15/08/2015 e foi finalizado em 28/11/2015. A primeira etapa foi avaliada durante 42 dias e a segunda etapa durante 63 dias, totalizando 105 dias. Na primeira etapa, o inoculante ? base de Azospirillum teve influ?ncia em todos os par?metros analisados. Na segunda etapa, os par?metros n?mero de folhas, rela??o parte a?rea/peso de raiz, massa fresca da raiz, massa seca da raiz e volume de raiz foram influenciados positivamente pelo tratamento.Podemos citar tr?s poss?veis causas para n?o haver signific?ncia nas outras an?lises: falta de ?gua em determinados per?odos; alta temperatura/insola??o no per?odo de fechamento das plantas, o que pode ter abortado as hastes florais; e a competi??o com as plantas espont?neas existentes no local, principalmente ciper?ceas. O uso do inoculante com a estirpe Sp245 de Azospirillum demostrou ser uma alternativa vi?vel no cultivo de statice (Limonium sinuatum) como flor de corte.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-04-26T14:05:04Z No. of bitstreams: 1 2012 - Patricia Gon?alves Galv?o.pdf: 5289496 bytes, checksum: 8dcb41bc971793cc8b4cdf383401ade4 (MD5) / Made available in DSpace on 2017-04-26T14:05:05Z (GMT). No. of bitstreams: 1 2012 - Patricia Gon?alves Galv?o.pdf: 5289496 bytes, checksum: 8dcb41bc971793cc8b4cdf383401ade4 (MD5) Previous issue date: 2012-03-01 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The objective of this study was to evaluate the protein profile expression of G. diazotrophicus PAL5 and its defective mutant in the indole compound production (lao-) grown in the presence or absence of tryptophan through 2DE-PAGE technique. The spectrometric analysis allowed the identification of 24 differentially expressed proteins. The majority of the proteins down regulated in the wild type PAL5 cultivated with tryptophan as compared to the cultivation without the amino acid belonged to the category of transductional modification, protein turnover and chaperones. For the mutant lao- grown in the same conditions, the majority of the proteins that presented differential expression belonged to the category of production and conversion of energy. In addition, the majority of the protein differentially expressed in the mutant lao- as compared to the wild-type PAL5 strains belonged to carbohydrates metabolism and transport. On the other hand, no proteins related to the tryptophan biosynthesis were detected in any condition, possibly due to the low yield of the proteins during the spectrometric analysis. Furthermore, mutants lao- and nif- of G. diazotrophicus were used for inoculation of micropropagated sugarcane plants in order to determine the influence of auxins produced by the bacteria in the plant growth promotion in comparison with PAL5. The first experiment, carried out in hydroponic conditions for 10 days showed a significant inoculation effect of the wild type on plant shoot. The other experiment was conducted in a period of 120 days in pots containing sand:vermiculite substrate fertilized with 30 and 60 ppm with ammonium sulphate enriched with 15N. The plants were inoculated in vitro with the wild type and mutants lao- and nif-, and the results showed a visual difference in the roots inoculated with PAL5 that showed higher volume suggesting a higher number of secondary roots and root hairs. On the other hand, the plants inoculated with the lao- mutant were ticker and showed lower number of secondary roots and root hairs. The shoot biomass of plants inoculated with PAL5 was higher than those inoculated with the mutant strains for both N dose, however the difference was not significant. Plants grown with 60 kg N dose and inoculated with the mutants showed lower accumulation of dry shoot mass than plants inoculated with the wild type strain. In conclusion, the present study showed the occurrence of several differentially expressed proteins either in the wild type strain or in the mutant lao- grown in LGI-P with and without tryptophan. The role played by these proteins in the metabolism of the bacteria requires additional studies, including different growth conditions. In addition, the inoculation of micropropagated sugarcane plants suggested a hormonal effect of the bacteria mainly on the root development e consequently in the N use efficiency. However, the size of the pots may have limited the plant development, suggesting that new experiments should be carried out in more appropriated conditions to confirm the influence of the indol production and the BNF during the association of the G. diazotrophicus and sugarcane plants / Este estudo teve por objetivo avaliar o perfil de express?o de prote?nas de G. diazotrophicus PAL5 e seu mutante defectivo na produ??o de compostos ind?licos (lao-) cultivados na presen?a e aus?ncia de triptofano atrav?s da t?cnica de 2DE-PAGE. A an?lise por espectrometria de massa permitiu a identifica??o de 24 prote?nas diferencialmente expressas. A maioria das prote?nas com a express?o diminu?da em PAL5 cultivada em meio com triptofano em rela??o ao meio de cultivo sem esse amino?cido pertenceu ? categoria modifica??o p?s-traducional, turnover de prote?nas e chaperonas. No mutante lao- cultivado nas mesmas condi??es, a maioria das prote?nas que apresentaram express?o diferencial pertencia ? categoria produ??o e convers?o de energia. Em adi??o, a maioria das prote?nas que foram diferencialmente expressas no mutante lao- em compara??o com a estirpe selvagem PAL5 pertencia ? categoria metabolismo e transporte de carboidratos. Por outro lado, n?o foram observadas prote?nas relacionadas ? bioss?ntese de triptofano em nenhuma condi??o analisada possivelmente devido ao baixo rendimento das identifica??es por espectrometria. Al?m das an?lises dos perfis de prote?nas, os mutantes lao- e nif- de G. diazotrophicus foram inoculados em plantas de cana-de-a??car micropropagadas com o objetivo de determinar a influ?ncia das auxinas na promo??o do crescimento dessa cultura em compara??o com a estirpe selvagem PAL5. O primeiro experimento, conduzido em condi??es de hidroponia pelo per?odo de 10 dias, mostrou efeito significativo da inocula??o da estirpe selvagem na promo??o de crescimento da parte ?rea das plantas, enquanto que o mutante lao-, n?o diferiu estatisticamente do controle n?o inoculado. O outro experimento, foi conduzido por 120 dias em vasos com substrato areia:vermiculita contendo 30 ou 60 ppm de sulfato de am?nio enriquecido com 15N e as pl?ntulas foram inoculadas in vitro. Os resultados mostraram uma diferen?a visual nas ra?zes das plantas inoculadas com PAL5, que se mostraram mais volumosas, aparentando um n?mero mais elevado de ra?zes secund?rias e p?los radiculares. J? as plantas inoculadas com lao- apresentaram ra?zes mais grossas, com um n?mero muito reduzido de ramifica??es ou p?los radiculares. A biomassa seca da parte a?rea das plantas inoculadas com PAL5 foi superior ?quelas inoculadas com as estirpes mutantes para as duas doses de nitrog?nio, por?m essa diferen?a n?o foi significativa. N?o foram observadas evid?ncias de contribui??o da FBN, por?m as plantas inoculadas com PAL5 foram menos eficientes na recupera??o do N fertilizante. Em conclus?o, o presente estudo mostra a ocorr?ncia de diversas prote?nas diferencialmente expressas tanto na estirpe selvagem como em lao- quando crescidas na presen?a e aus?ncia do amino?cido triptofano. A defini??o do papel dessas prote?nas no metabolismo da bact?ria requer estudos adicionais, inclusive em diferentes condi??es de cultivo. Em adi??o, a inocula??o dessas bact?rias em plantas de cana-de-a??car mostrou o efeito hormonal da bact?ria no desenvolvimento das ra?zes e, por conseguinte na maior efici?ncia de uso do N aplicado. Entretanto, dado a limita??o de espa?o f?sico dos vasos para o desenvolvimento das plantas, sugere-se a realiza??o de novos experimentos, em condi??es mais apropriadas, para confirmar a influ?ncia da produ??o de ?ndoles e da FBN durante a associa??o da bact?ria com as plantas de cana-de-a??car.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-08-17T19:59:36Z No. of bitstreams: 1 2012 - Elson Barbosa da Silva Junior.pdf: 1890259 bytes, checksum: 16078598a033a11572fd35aadc93d40b (MD5) / Made available in DSpace on 2017-08-17T19:59:36Z (GMT). No. of bitstreams: 1 2012 - Elson Barbosa da Silva Junior.pdf: 1890259 bytes, checksum: 16078598a033a11572fd35aadc93d40b (MD5) Previous issue date: 2012-02-24 / CAPES / The planting of cowpea [Vigna unguiculata (L.) Walp.] is growing in the Midwest of Brazil, which is characterized by a technified agriculture, with an average yield of 1000 kg ha-?. New technologies of inoculation as carriers and the agricultural practice of pre inoculation are presented as alternative to current technologies. Thus the objective of this study was to evaluate technologies for cowpea inoculation in the Midwest region of Brazil. The quality of the polymeric IPC 2.2 inoculant was evaluated for the cells survival at 180 days of storage and the ability to maintain viable cells after five weeks of inoculation. The agronomic performance of the polymeric inoculant and the pre inoculation were compared with technologies already recommended, in three different experiments with the same cultivate (BRS Guariba) in areas of Embrapa Agrobiologia (Serop?dica-RJ), Embrapa Agrossilvipastoril (Sinop-MT) and in the New Horizon farm ? Seeds Tomazetti (Primavera do Leste, MT). The contribution of biological nitrogen fixation was quantified by 15N natural abundance delta (?) in the two experiments in Mato Grosso State. In the experiment at Embrapa Agrobiologia the polymeric inoculant IPC 2.2 was compared with peat and liquid carriers, plus nitrogen treatments with 50 and 80 kg N ha-? and the absolute treatment. In the experiment at Embrapa Agrossilvopastoril the treatments were pre inoculated with polymeric inoculant IPC 2.2 and peat carrier, with 0, 1, 2 and 5 weeks before planting for both inoculants plus addition of nitrogen (70 kg N ha-?), and the absolute treatment. The assay at the New Horizon farm was in a central pivot area of 60 ha with the strains: BR3267 in the polymeric and liquid carriers; BR3262 in polymeric; and a consortium of four recommended strains (BR3267, BR3262, and BR3301 BR3302) in the polymeric carrier. The polymer showed inoculant cell concentration higher than 109 cells per gram after 180 days, and in the field experiment the formulation IPC 2.2 provided yield above the reference treatment and equal to the N treatment, and the peat and liquid carriers. The pre-inoculation with the polymeric blend with five weeks had the highest nodule dry mass, however it did not differ from the N control and the absolute, and the treatments did not differ for the productivity. In the essay at the Novo Horizonte farm the inoculation with the consortium of four strains had the highest nodule dry mass, and it differed from the BR3267 in liquid carrier and the BR3262. The contribution of biological nitrogen fixation did not exceed 50%, and the highest averages were obtained with 48% and 39% of N originated from biological fixation, respectively in the pre inoculation essay (14 days in the peat carrier), and with the BR3262 at the New Horizon farm with the polymeric carrier. Thus, it is concluded that the polymer inoculant might be recommended for the traditional form inoculation of the cowpea crop, and inoculation should be a common practice, since it was proven that with the no inoculated seeds there was no biological nitrogen fixation. / O plantio de feij?o-caupi [Vigna unguiculata (L.) Walp.] vem crescendo no Centro-Oeste do Brasil, que se caracteriza por uma agricultura tecnificada, com m?dia de produtividade de 1000 kg ha-?. Novas tecnologias de inocula??o, como ve?culos e uma pr?tica agr?cola de pr? inocula??o apresentam-se como alternativa as tecnologias atuais. Assim o objetivo deste trabalho foi avaliar tecnologias de inocula??o de feij?o-caupi na regi?o Centro-Oeste do Brasil. Foi avaliada a qualidade do inoculante polim?rico IPC 2.2, quanto ? sobreviv?ncia de c?lulas aos 180 dias de armazenamento e quanto ? capacidade de manter c?lulas vi?veis ap?s cinco semanas de inocula??o. O desempenho agron?mico do inoculante polim?rico e da pr? inocula??o foi comparado com as tecnologias j? recomendadas em tr?s experimentos distintos com a mesma cultivar BRS Guariba nas ?reas da Embrapa Agrobiologia (Serop?dica-RJ), Embrapa Agrossilvipastoril (Sinop-MT) e na fazenda Novo Horizonte- Sementes Tomazetti ( Primavera do Leste-MT). Foi quantificada a contribui??o da fixa??o biol?gica de nitrog?nio pela abund?ncia natural do delta 15N (?) nos dois experimentos no Mato Grosso. No experimento na Embrapa Agrobiologia o inoculante polim?rico IPC 2.2 foi comparado com os ve?culos turfoso e l?quido, mais os tratamentos nitrogenados com 50 e 80 kg N ha-? e o absoluto. No experimento na Embrapa Agrossilvipastoril os tratamentos foram pr? inoculados com inoculante polim?rico IPC 2.2 e turfoso com 0, 1, 2 e 5 semanas antes do plantio para ambos inoculantes, mais o tratamento nitrogenado (70 kg N ha-?) e o absoluto. O ensaio na fazenda Novo Horizonte foi em ?rea de piv? central de 60 ha, com as estirpes: BR3267 em ve?culo polim?rico e l?quido; BR3262 em ve?culo polim?rico; e o cons?rcio das quatro estirpes recomendadas (BR3267, BR3262, BR3301 e BR3302) em ve?culo polim?rico. A formula??o IPC 2.2 manteve concentra??o de c?lulas superior a 109 c?lulas por grama ap?s 180 dias e no campo ela proporcionou uma produtividade superior ao tratamento absoluto e igual a dos tratamentos nitrogenados, ve?culos turfoso e l?quido. A pr?-inocula??o com a mistura polim?rica com cinco semanas obteve a maior massa de n?dulos secos, por?m n?o diferiu do controle nitrogenado e absoluto, assim como na produtividade os tratamentos n?o diferiram entre si. No ensaio na fazenda Novo Horizonte a inocula??o com o cons?rcio das quatro estirpes obteve a maior massa de n?dulos secos e diferiu da BR3267 em ve?culo l?quido e da BR3262. A contribui??o da fixa??o biol?gica de nitrog?nio n?o ultrapassou os 50%, obtidas as maiores m?dias com 48% e 39% do N oriundo da fixa??o biol?gica respectivamente nos ensaios de pr?-inocula??o (14 dias no ve?culo turfa) e com a BR3262, na fazenda Novo Horizonte e em ve?culo polim?rico. Assim, se conclui que o inoculante polim?rico pode ser recomendado para inocula??o tradicional do feij?o caupi e que a inocula??o da cultura deve ser uma pr?tica corriqueira, uma vez que foi comprovado que com sementes n?o inoculadas n?o houve fixa??o biol?gica de nitrog?nio.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-10-04T17:35:36Z No. of bitstreams: 1 2013 - Fernanda Santana de Paulo.pdf: 1349643 bytes, checksum: 8da583439baf6dcac85c9ddb6af5ffbc (MD5) / Made available in DSpace on 2017-10-04T17:35:36Z (GMT). No. of bitstreams: 1 2013 - Fernanda Santana de Paulo.pdf: 1349643 bytes, checksum: 8da583439baf6dcac85c9ddb6af5ffbc (MD5) Previous issue date: 2013-02-27 / CAPES / Embrapa / The cowpea bean is a legume that has a wide economic potential, but it has not been explored efficiently. For this reason it has shown a yield potential far from the expectations of a so efficient legume in NBF as can be. The objective of this study is to obtain appropriate levels of P, Co, Mo nutrients. It is believed that these nutrients can optimize nodulation, nitrogen biological fixation and the growth of cowpea bean plants in representative soils from Cerrado, Mato Grosso. Based on the chemical analysis from the soil cited before, calculations for fertilization and liming were made. From the results obtained, were established doses for the elements of the present work (P, Co and Mo). The experiment was conducted in a greenhouse conditions and performed in a randomized block design. It was used a factorial arrangement 2 x 5 (2 soils; five doses of each element of the present study ? inoculated with 3262 strain) + 1 + 3 replicates. The additional treatment (+1) was included to evaluate crop performance when applied 50 Kg.ha-1 of nitrogen. The study was split in two trials. In the first trial, different dosages of phosphorous were tested: control (without Phosphorus application); low (44 mg.vase-1 of P), medium (88 mg.vase-1 of P), medium-low (130mg.vase-1 of P) and high (175 mg.vaso-1 of P). These tests were made to obtain a optimum dosage. In the second trial, cobalt and molybdenum nutrient was tested as the following levels respectively: 0, 2, 3, 4, 6 mg and 0, 8, 16, 32 and 64 mg. In the thirty-fifth day, the experiment developed in a greenhouse was collected. From the plants were evaluated height and plant diameter, nodulation (dry matter and number of nodules), shoot dry matter, root dry matter, chlorophyll content and N accumulation in shoot. The 88mg of P.vase-1 dosage that corresponds to 200 kg.ha-1 of P2O5 and the medium-high Cobalt and Molybdenum dosage that corresponds the application of 32g of Mo.ha-1 + 4g.ha-1 of Co provided higher nodulation in cowpea bean plants with consequent increase of biomass and nitrogen accumulation. The site where soil was collected had influence on the development and on the nodulation of the cowpea bean crop, when submitted to different dosages of P and Co+Mo. / O feij?o-caupi ? uma leguminosa de vasto potencial econ?mico, por?m n?o est? sendo explorado de maneira eficiente apresentando-se com potencial produtivo bem aqu?m das expectativas de uma leguminosa t?o eficiente em FBN. Sendo ent?o, o objetivo deste trabalho obter os n?veis adequados dos nutrientes P, Co e Mo que otimizem a nodula??o, a fixa??o biol?gica de nitrog?nio e o crescimento de plantas de feij?o-caupi em solos representativos do Cerrado do Mato Grosso. Com base na an?lise qu?mica do solo, foram realizados os c?lculos da necessidade de calagem e aduba??o e foram estabelecidas as doses dos elementos em estudo (P, Co e Mo). O experimento foi realizado em condi??es de casa de vegeta??o. O delineamento experimental foi em blocos ao acaso em esquema fatorial 2 x 5 (2 solos; 5 doses de cada elemento de estudo ? inoculadas com a estirpe 3262) +1 e 3 repeti??es. O tratamento adicional (+1) foi inclu?do para avaliar o desempenho da cultura na dose de N proporcional a 50 kg.ha-1. O trabalho foi dividido em dois ensaios, onde primeiro foram testadas dosagens diferenciadas de f?sforo que foram denominadas: controle, (sem aplica??o de F?sforo); baixa (44 mg.vaso-1 de P), m?dia (88 mg.vaso-1 de P), m?dia-alta (130 mg.vaso-1 de P), alta (175 mg.vaso-1 de P) para a obten??o de uma dosagem ?tima. Na segunda fase, foram testados os micronutrientes Co nas doses: 0, 2,3 ,4 ,6 mg e Mo nas doses: 0, 8, 16, 32 e 64 mg. Em casa de vegeta??o, aos 35 dias foi feita a coleta, as vari?veis avaliadas foram: altura e di?metro da planta, nodula??o (massa de n?dulos secos e n?mero de n?dulos), massa seca da parte a?rea, massa seca da raiz, teor de clorofila e ac?mulo de N na parte a?rea. A dose de 88 mg de P. vaso-1, que corresponde a 200 kg.ha-1de P2O5 e at? a dose m?dia-alta de cobalto e molibd?nio com correspondente a aplica??o de 32 g de Mo. ha-1 + 4 g ha-1de Co proporcionou maior nodula??o das plantas de feij?o-caupi com consequente aumento da biomassa do vegetal e ac?mulo de nitrog?nio. O local de coleta de solo influenciou no desenvolvimento e nodula??o da cultura do feij?o-caupi, quando submetida a diferentes doses de P e Co+Mo.

?rea de concentra??o: Produ??o vegetal. / Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-01-03T16:10:54Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) debora_cintia_santos_avelar.pdf: 1075322 bytes, checksum: 956aee85f03869781bc0c12ae661e4f7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-01-31T16:25:33Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) debora_cintia_santos_avelar.pdf: 1075322 bytes, checksum: 956aee85f03869781bc0c12ae661e4f7 (MD5) / Made available in DSpace on 2017-01-31T16:25:33Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) debora_cintia_santos_avelar.pdf: 1075322 bytes, checksum: 956aee85f03869781bc0c12ae661e4f7 (MD5) Previous issue date: 2016 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A determina??o da dose de inoculante ? essencial para a obten??o de benef?cios da utiliza??o de fungos ectomicorr?zicos (FEM) em mudas de eucalipto. O objetivo deste estudo foi avaliar a melhor dose de inoculante miceliano de isolados de Pisolithus sp., encapsulados em gel de alginato de c?lcio, visando a promo??o da coloniza??o, nutri??o e do crescimento das mudas de clones de eucalipto em viveiro comercial. Em experimentos independentes e utilizando aduba??o fosfatada reduzida, foram produzidas mudas de dois clones de eucalipto (AEC 2034 e AEC 2233) inoculados com 9, 18 e 36 esferas de inoculante miceliano dos isolados f?ngicos D17, D216, D5 e D95 e um tratamento N?o-inoculado (fatorial 3x5). Al?m disso, foram feitos mais dois tratamentos adicionais com (Controle) e sem (Comercial) redu??o da aduba??o fosfatada do substrato e sem adi??o dos inoculantes. A dose de 36 esferas de inoculante, em geral, promoveu maiores ganhos para as mudas de eucalipto, como maior coloniza??o, massa seca da parte a?rea (MSPA) e de ra?zes (MSR) e teores de K, sendo algumas vezes maior que nas mudas do Controle e Comercial. A maior altura da parte a?rea e di?metro do coleto foram observados com o uso de 18 esferas. A coloniza??o ectomicorr?zica foi maior nas mudas inoculadas e considerada m?dia para os dois clones. A inocula??o pr?via nos dois clones n?o promoveu aumento nos teores de P em rela??o ao Comercial, no entanto para o clone AEC 2233 com a utiliza??o do D216, houve aumento nos teores de P em rela??o ao N?o-inoculado, sendo este isolado o mesmo que proporcionou uma das maiores porcentagens de coloniza??o, contribuindo para maior absor??o desse nutriente. Em rela??o aos isolados, a inocula??o com o D17 em geral aumentou a sobreviv?ncia, altura, MSPA, MSR e teores de K no AEC 2034 e a altura e di?metro no AEC 2233, mas isto foi dependente da dose e do clone de eucalipto. A dose de 18 esferas foi mais apropriada, pois, na maioria dos casos, os maiores benef?cios como sobreviv?ncia, altura, di?metro, MSPA, MSR, coloniza??o, teores de N e K foram obtidos com a adi??o de 18 ou mais esferas por mini-estaca e, em alguns casos, os par?metros altura e coloniza??o foram reduzidos nas maiores doses. O isolado D17 ? promissor para uso em programas de micorriza??o controlada em viveiros comerciais de mudas clonais de eucalipto e a dose de 18 esferas por mini-estaca ? a mais recomendada. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016. / The determination of the inoculum dose is essential for obtaining the benefits from the utilization of ectomycorrhizal fungi (EMF) in eucalypt saplings. The aim of this study was to evaluate the best dose of inoculum consists of mycelium from isolates Pisolithus sp., encapsulated in calcium alginate gel, aiming at promoting the colonization, nutrition and growth of the eucalypt rooted cuttings in commercial nursery. In independent experiments and using reduced phosphorus fertilization, were produced rooted cuttings from two eucalypts clones (AEC 2034 e AEC 2233) inoculated with 9, 8 and 36 beads of inoculum from fungal isolates D17, D216, D5 e D95 and one Non-inoculated treatment (factorial 3x5). Furthermore, two more additional treatments were performed with (Control) and without (Commercial) reduction of phosphorus fertilization of the substrate and without the addition of the inoculum.The dose of 36 inoculum beads, in general, has promoted higher benefits for the saplings, such as greater colonization, dry mass of shoot (DMS) and of roots (DMR) and K concentrations, sometimes being higher than in the saplings from Control and Commercial. The greater height of the shoot and collar diameter were observed with the use of 18 beads of inoculum. The ectomycorrhizal colonization was higher in inoculated saplings and considered average for the two clones. The previous inoculation in both clones did not promote an increase in P concentrations in relation to Commercial, however for the AEC 2233 clone with the utilization of D216, there was an increase in the P concentrations in relation to Non-inoculated, this being the same isolate that provided one of the greatest percentages of colonization, contributing to a higher absorption of this nutrient. Regarding the isolates, the inoculation with D17 in general increased the survival, height, DMS, DMR and K concentrations in AEC 2034, and height and diameter in AEC 2233, but this was dependent on the dose and on the eucalypt clone. The dose of 18 beads of inoculum was most appropriate, because in most cases, the greatest benefits as survival, height, diameter, DMS, DMR, colonization, N and K concentrations were obtained with addition of 18 or more beads of inoculum by mini-cutting and, in some cases, the parameters height and colonization were reduced with the highest doses. The isolate D17 is promising for its use in controlled mycorrization programs in commercial nurseries of eucalypt rooted cuttings and the dose of 18 beads by mini-cutting is the most recommended.

Made available in DSpace on 2016-04-28T14:58:31Z (GMT). No. of bitstreams: 1 2003- Daniele Cristina Costa Sabino.pdf: 534915 bytes, checksum: c4045f2c9f540e49bb6c91877c6845ff (MD5) Previous issue date: 2003-03-26 / Funda??o Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro / Rice is colonized by diazotrophic bacteria that can act as root growth promoting, as well as to increase plant nutrition throw the biological nitrogen fixation process. These associations can modify the plant physiology since they compete for carbohydrate and other compounds necessary to host plants. The aim of this work was to evaluate the influence of the endophytic diazotrophic bacteria, Burkholderia brasilensis and Herbaspirillum seropedicae on nitrogen metabolism of two rice cultivars (IR42 and IAC4440). The experiment was carried out in pots containing 3 kg of soil, fertilized with 3 nitrogen levels (0, 40, and 80 kg N ) as (NH4)2SO4, and three inoculation treatments (H. seropedicae (ZAE94), B. brasilensis (M130) and the mixture of both). The plants were harvested at vegetative (45 days of inoculation) and flowering stages (according to the cycle of each cultivar). Bacterial counting, dry matter, N content and the concentration of nitrogen compounds were measured at each harvest. The results showed no statistical differences on bacterial counting. The number of root bacteria was higher than in shoots. A population reduction occurred on the flowering stage as compared to vegetative one. Cultivar IR42 responded to inoculation more noticiable than IAC4440. The dry matter accumulation of the IR42 cultivar at the vegetative stage varied with inoculation and nitrogen dose applied. This cultivar accumulated higher amounts of nitrogen when inoculated with ZAE94 strain, independent of the nitrogen applied. Concentrations of nitrate, free amino acid and soluble sugar in the aerial parts were higher in plants fertilized with 40kgN/ha and inoculated with the mixture of bacteria. These results suggested that the diazotrophic bacteria inoculation could modify the plants nitrogen metabolism and theses effects were variable according to the rice cultivars and nitrogen fertilization level applied. / O arroz (Oryza sativa L.) ? colonizado por bact?rias diazotr?ficas que, podem atuar como promotoras de crescimento das ra?zes, assim como melhorar a nutri??o da planta atrav?s do N2 fixado biologicamente. No entanto, estas associa??es podem modificar a fisiologia das plantas, uma vez que as bact?rias diazotr?ficas para fixarem o nitrog?nio necessitam de carboidratos e outros componentes, competindo, deste modo, com as plantas hospedeiras. Assim sendo, este trabalho teve como objetivo avaliar a influ?ncia da inocula??o de bact?rias diazotr?ficas endof?ticas Burkholderia brasilensis e Herbaspirillum seropedicae no metabolismo de nitrog?nio de duas cultivares de arroz adubadas ou n?o com nitrog?nio mineral. Um experimento em condi??es de vasos foi realizado na ?rea experimental da Embrapa Agrobiologia com as cultivares de arroz IR42 e IAC4440. A inocula??o consistiu no uso das estirpes ZAE94 de Herbaspirillum seropedicae, e M130 de Burkholderia brasilensis , a mistura destas duas estirpes e da testemumha (controle n?o inoculado). A aduba??o na forma de (NH4)2SO4, foi aplicada nas concentra??es de 0, 40 e 80 kgN/ha. As coletas foram realizadas no per?odo vegetativo (45 dias) e no florescimento (de acordo com o ciclo de cada cultivar). Os par?metros avaliados foram a presen?a das bact?rias nos tecidos das plantas, o ac?mulo de mat?ria seca da parte a?rea, o ac?mulo de N, e a concentra??o dos compostos nitrogenados na planta. Os resultados mostraram que n?o houve diferen?as nas popula??es das bact?rias em fun??o da inocula??o, por?m, o n?mero de bact?rias na raiz foi ligeiramente maior do que na parte a?rea. Foi tamb?m observado uma redu??o na popula??o no per?odo de florescimento, em rela??o ao per?odo vegetativo. De um modo geral, a cultivar IR42 apresentou maiores respostas ? inocula??o, quando comparada com a cultivar IAC4440. O ac?mulo de mat?ria seca da cultivar IR42 no per?odo vegetativo, variou com a inocula??o e a dose de nitrog?nio aplicada. Por?m, a percentagem de nitrog?nio (%N) acumulada na parte a?rea destas plantas, foi maior nos tratamentos inoculados com a estirpe ZAE94, independente da dose de nitrog?nio aplicada. As plantas que receberam 40 kgN/ha e foram inoculadas com a mistura, apresentaram as maiores concentra??es de nitrato, amino?cidos livres e a??cares sol?veis na parte a?rea. Os resultados obtidos sugerem que a inocula??o das bact?rias diazotr?ficas modifica o metabolismo de nitrog?nio e que este efeito foi diferenciado em fun??o da variedade e da dose de nitrog?nio aplicada.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-09-19T12:12:01Z No. of bitstreams: 1 2010 - Rafael Sanches Pacheco.pdf: 669093 bytes, checksum: 3ca2e98e7a5ac781dba791f2e1bfda70 (MD5) / Made available in DSpace on 2018-09-19T12:12:01Z (GMT). No. of bitstreams: 1 2010 - Rafael Sanches Pacheco.pdf: 669093 bytes, checksum: 3ca2e98e7a5ac781dba791f2e1bfda70 (MD5) Previous issue date: 2010-08-10 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico, CNPq, Brasil. / Nutrient concentration in the seeds can affect the initial development, biological nitrogen fixation (BNF) and yield of plants. Common bean (Phaseolus vulgaris L.) has been considered as a species with low capacity of BNF as compared to other grain legumes, but results demonstrate the potential of BNF to supply the N demand of the crop and to achieve adequate yields. The objective of this study was to evaluate the effect of seed concentrations of Mo and P on biomass production, nodulation and grain yield of common bean at different sources of N supply. The field experiment was conducted between April and July 2009, in the Santa Monica Farm of Embrapa Gado de Leite in the municipality of Valen?a ? Rio de Janeiro State. The experimental design was a split-plot 4x4 factorial randomized block with 5 replicates. The main plots consisted of four N sources: control without N, mineral N fertilization with 20 kg N ha-1 at sowing + 40 kg N ha-1 in cover, inoculation with the commercial strain of rhizobia, rhizobia inoculation + 40 kg N ha-1 in cover. The subplots consisted of four combinations between concentrations of P and Mo in the seeds (low P low Mo, low P high Mo, Mo high P low, high-P high-Mo). The seeds of cultivar Carioca were originating from a field experiment when 5 kg P ha-1 and 120 g Mo ha-1 were foliar sprayed at 52 and 71 days after emergence (DAE). In each subplot, plant biomass were sampled at 30, 42 and 57 DAE, and root, shoot and nodules dry mass, the number of nodules and the accumulation of N in the shoot were determined. Grains were harvested at 90 DAE, and grain yield, yield components, N and Mo concentratios in the grains were measured. Interactions between the N sources and the concentrations of P and Mo in the seeds were not significant in the three sampling times, except for shoot dry mass and number of nodules at 57 DAE. Seeds with low P and high Mo increased shoot dry matter and grain yield of beans. Mineral N fertilizer increased root mass in the early growth stages. The rhizobia inoculation increased bean nodulation. There was a small effect of the seeds with high P concentration in the accumulation of N and mass of shoots at 57 DAE. Mineral N fertilizer increased N accumulation in the shoots at 57 DAE. The inoculation + 40 kg N ha-1 in cover gave similar grain yield and N accumulation as the application of 60 kg N ha-1, both treatments superior to the control. The use of seeds enriched with P and Mo, associated with the inoculation of seeds and the application of N in cover, could maximize the process of biological nitrogen fixation in the common bean crop. / A concentra??o de nutrientes nas sementes pode afetar o desenvolvimento inicial, a fixa??o biol?gica de nitrog?nio (FBN) e a produ??o de gr?os das plantas por elas geradas. O feijoeiro (Phaseolus vulgaris L.) tem sido considerado uma esp?cie de baixa capacidade de FBN em compara??o com outras leguminosas de gr?o, mas resultados demonstram o potencial da FBN em suprir a demanda de N do feijoeiro e obter boas produtividades. O objetivo deste estudo foi avaliar o efeito dos teores de P e Mo em sementes na produ??o de biomassa, nodula??o e no rendimento de gr?os de feijoeiro sob diferentes fontes de fornecimento de N. O experimento foi conduzido em condi??es de campo entre abril e julho de 2009, na Fazenda Santa M?nica da Embrapa Gado de Leite, no munic?pio de Valen?a ? RJ, em delineamento em blocos ao acaso em fatorial 4x4 com 5 repeti??es em parcelas subdivididas. As parcelas foram compostas por quatro fontes de N: testemunha absoluta sem N; aduba??o com N mineral com 20 kg N ha-1 no plantio + 40 kg N ha-1 em cobertura; inocula??o com a estirpe comercial de riz?bio recomendada para a cultura; inocula??o com estirpe comercial + 40 kg N ha-1 em cobertura. As subparcelas foram compostas por quatro combina??es entre teores de P e de Mo na semente (baixo P baixo Mo, baixo P alto Mo, alto P baixo Mo, alto P alto Mo). As sementes da cultivar Carioca utilizadas foram oriundas de um experimento de campo onde foram efetuadas aplica??es foliares com 5 kg P ha-1 e 120 g Mo ha-1 aos 52 e 71 dias ap?s emerg?ncia (DAE). Em cada subparcela foram efetuadas tr?s amostragens de biomassa, aos 30, 42 e 57 DAE, determinando-se a massa seca de raiz, parte a?rea e n?dulos, o n?mero de n?dulos e a acumula??o de N na parte a?rea. A colheita de gr?os foi efetuada aos 90 DAE, determinando-se o rendimento de gr?os, os componentes de produ??o e o teor de N e de Mo nos gr?os. As intera??es entre as fontes de N e os teores de P e Mo das sementes, nas tr?s ?pocas de coleta, n?o foram significativas, exceto para massa seca de parte a?rea e n?mero de n?dulos aos 57 DAE. As sementes com baixo P e alto Mo foram as que promoveram maior massa de parte a?rea e tamb?m a maior produ??o de gr?os de feij?o. A aduba??o com N mineral promoveu maior massa radicular nos est?gios iniciais de crescimento. A inocula??o das sementes com riz?bio aumentou a nodula??o do feijoeiro. Houve um pequeno efeito dos altos teores de P nas sementes na acumula??o de N e de biomassa da parte a?rea aos 57 DAE. A aplica??o de N mineral promoveu maior ac?mulo da N na parte a?rea aos 57 DAE. A inocula??o combinada com 40 kg N ha-1 em cobertura forneceu rendimento de gr?os e acumula??o de N nos gr?os similar ? aplica??o de 60 kg N ha-1, por sua vez superiores ? testemunha. O uso de sementes enriquecidas com P e Mo, associada com a inocula??o de sementes e a aplica??o de N em cobertura, podem maximizar o processo de fixa??o biol?gica de nitrog?nio na cultura do feijoeiro.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2018-09-19T14:33:33Z No. of bitstreams: 1 2016- Rodolfo Cond? Fernandes.pdf: 1891258 bytes, checksum: d3f6f4175d22736ad9299a9acbcef3c7 (MD5) / Made available in DSpace on 2018-09-19T14:33:33Z (GMT). No. of bitstreams: 1 2016- Rodolfo Cond? Fernandes.pdf: 1891258 bytes, checksum: d3f6f4175d22736ad9299a9acbcef3c7 (MD5) Previous issue date: 2016-07-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Cap. I The objective of this study was to evaluate the growth, nodulation, production and technological analysis of grains of common bean cultivars under organic and conventional fertilization. Two field experiments were conducted at the research site of Embrapa Agrobiologia, Serop?dica - RJ, during April and July 2013 and April and August 2014. A 4x4 factorial split plot design (4 bean cultivars and 4 fertilization) with four replications was used, the fertilizations in the plots and cultivars in the subplots. The cultivars were Apor?, Constanza, Manteig?o e Valente. The fertilizer treatments were: absolute control, chemical fertilizer and two levels of cattle manure applied at planting and castor seed meal applied at top dressed. The subplots were 4 lines of 4 m spaced by 0.5 m, using 12 seeds per meter. The seeds were inoculated with rhizobium commercial inoculant for bean with peat as a means of inoculation. Plants were sampled at two growth stages, for biomass and nodulation at flowering, and for grain yield and yield components at maturity of each cultivar. In order to determine the influence of fertilization on the cooking time and grain water absorption, a field experiment was carried out When? with 6 bean cultivars with chemical or organic fertilization. In both years, the lower dose of organic fertilizer had higher nodulation than higher organic dose and the control treatment and chemical fertilizer did not differ between themselves and had the lowest values for number and mass of nodules. In both years, cultivars Apor? and Valente showed higher number of pods per plant, number of seeds per pod and harvest index than the other cultivars. However, there were no differences between cultivars for grain yield in the two years. Organic fertilization associated with inoculation in the two years provided yields ranging from 209.9 to 262.4 g m-2, considered satisfactory and similar to those obtained by chemical fertilizer, arising as a promising technology. The organic fertilization provided N, P, K and Mg contents in grains similar to treatment with chemical fertilization. At experiment of 2015, the percentage of water absorption in grains and baking time was higher in beans that received chemical fertilizers for most cultivars Cap. II The production of common bean (Phaseolus vulgaris L.) in small growing areas, coupled with the use of technologies that provide a reduction in the production cost and increase the economic returns of farmers, demand the identification of cultivars better suited to this type of management and accepted in the regional consumer market. The objective of this study was to evaluate the production of different bean cultivars of different types of commercial grain and the techniques of low cost management used by family farmers in Cachoeiras de Macacu region - RJ. Two experiments were conducted between April and July 2014 (winter crop) in two family farm properties. The sites were "Dois Irm?os" and "Santa Monica". Five bean cultivars were evaluated at ?Dois Irm?os? (Constanza, Kaboon, Manteig?o, Radiante and Valente) and two fertilization treatments (chemistry fertilizer at farmers? applied rate and organic fertilizer). At the Santa Monica site 6 cultivars were evaluated (Valente, Constanza, Radiante, Bolinha, Pitanga and Jalo Precoce) without any fertilization, with four replicates per treatment. The plots were spaced lines of 4 m by 0.5 m, using 12 seeds per meter. Seeds were inoculated with peat based commercial rhizobia inoculants. At maturity, grains of each cultivar were harvested from an area of 2 m2 in the two central lines of each plot for productivity assessment. Grain yield of the five cultivars did not differ among the fertilizer treatments at ?Dois Irm?os?. Grain production of the six cultivars did not differ from each other at ?Santa Monica?. The Valente and Radiante cultivars reached the highest production for the two sites; ?Dois Irm?os? and ?Santa Monica?, respectively. In general, all cultivars showed good performance for the different types of management, with average yield above 2000 kg ha-1 in the two properties. Technique of inoculation of seeds with rhizobia was well evaluated by farmers by the ease implementation and low cost of deployment. / Cap. I O objetivo deste trabalho foi avaliar o crescimento, produ??o e a an?lise tecnol?gica de gr?os de cultivares de feijoeiro comum (Phaseolus vulgaris), sob aduba??o org?nica e convencional. Dois experimentos foram realizados em campo na Embrapa Agrobiologia, em Serop?dica ? RJ, entre abril e julho de 2013 e abril e agosto de 2014, em delineamento em blocos ao acaso, em esquema fatorial 4x4 em parcelas subdivididas (4 cultivares e 4 tipos de aduba??o) com quatro repeti??es, as aduba??es dispostas nas parcelas e as cultivares nas subparcelas. Foram utilizadas as cultivares Apor?, Constanza, Manteig?o e Valente. Os tratamentos de aduba??o foram testemunha absoluta, aduba??o qu?mica e duas doses de aduba??o org?nica com esterco bovino no sulco de plantio e torta de mamona em cobertura. As subparcelas foram constitu?das por 4 linhas de 4 m espa?adas de 0,5 m, empregando-se 12 sementes por metro linear. As sementes foram inoculadas com inoculante comercial contendo riz?bio para feijoeiro com turfa como meio de inocula??o. Efetuaram-se duas amostragens, uma de biomassa e nodula??o na flora??o e outra na matura??o dos gr?os de cada cultivar para avalia??o do rendimento e componentes de produ??o. No mesmo campo experimental, com o objetivo de se determinar a influ?ncia do manejo sobre a qualidade tecnol?gica dos gr?os, instalou-se um ensaio a campo, entre setembro e dezembro de 2015, para produzir gr?os de 6 cultivares comerciais de feijoeiro comum, sob 2 tipos de aduba??o, qu?mica e org?nica. Comparando-se as m?dias da nodula??o quanto ao tipo de aduba??o Nos dois anos, a menor dose de aduba??o org?nica apresentou valores superiores de nodula??o que a maior dose org?nica e, por sua vez os tratamentos testemunha e aduba??o qu?mica n?o diferiram entre si e apresentaram os menores valores para o n?mero e massa de n?dulos. Nos dois anos, as cultivares Apor? e Valente apresentaram valores superiores de n?mero de vagens por planta, n?mero de gr?os por vagem e ?ndice de colheita que as demais. Entretanto n?o houve diferen?as das cultivares para a 19 produ??o de gr?os os dois anos avaliados. A aduba??o org?nica associada ? inocula??o nos dois anos proporcionou rendimentos variando de 209,9 a 262,4 g m-2, considerados satisfat?rios e semelhantes aos obtidos pela aduba??o qu?mica, mostrando-se uma tecnologia promissora. As aduba??es org?nicas proporcionaram conte?dos de N, P, K e Mg nos gr?os similares ao tratamento com aduba??o qu?mica. No experimento de 2015, a porcentagem de absor??o de ?gua nos gr?os e o tempo de cozimento foram menores quando os gr?os foram provenientes da aduba??o org?nica para a maioria das cultivares. Cap. II A produ??o de feijoeiro comum (Phaseolus vulgaris L.) em pequenas ?reas de cultivo, aliado ao uso de tecnologias que proporcionem redu??o no custo de produ??o e aumento do retorno econ?mico para o agricultor, demanda a identifica??o de cultivares melhor adaptadas a este tipo de manejo e aceita??o no mercado consumidor regional. Objetivou-se com o presente trabalho avaliar a produ??o de diferentes cultivares de feijoeiro, de diferentes tipos comerciais de gr?o, e a utiliza??o t?cnicas de manejo de baixo custo por agricultores familiares da regi?o de Cachoeiras de Macacu ? RJ. Dois experimentos foram realizados entre abril e julho de 2014 (safra de inverno), em duas propriedades sob agricultura familiar, os s?tios ?Dois Irm?os? e ?Santa M?nica?. No s?tio Dois Irm?os adotou-se o delineamento experimental de blocos ao acaso no esquema de parcelas subdivididas 2 x 5 (2 aduba??es e 5 cultivares), as aduba??es dispostas nas parcelas e as cultivares nas subparcelas. Foram avaliadas as cultivares (Constanza, Kaboon, Manteig?o, Radiante, Valente) sob os tratamentos de aduba??o (qu?mica praticada pelo agricultor e org?nica), com quatro repeti??es por tratamento. Para o s?tio Santa M?nica, adotou-se um delineamento em blocos ao acaso com quatro repeti??es, onde foram avaliadas 6 cultivares (Valente, Constanza, Radiante, Bolinha, Pitanga e Jalo Precoce) sem nenhum tipo de aduba??o. Em ambos experimentos, as parcelas foram constitu?das por linhas de 4 m espa?adas de 0,5 m, empregando-se 12 sementes por metro linear, cujas sementes foram inoculadas com inoculante comercial com riz?bio para feijoeiro, com turfa como meio de inocula??o. Na matura??o dos gr?os de cada cultivar, foi colhida uma ?rea de 2 m2 nas duas linhas centrais de cada parcela para avalia??o da produtividade. No S?tio dois Irm?os, a produ??o de gr?os das cinco cultivares n?o diferiu entre os tratamentos aduba??o org?nica e aduba??o qu?mica. No S?tio Santa M?nica, a produ??o de gr?os das seis cultivares n?o diferiu entre si. As cultivares Valente e Radiante atingiram a maior produ??o no S?tio Dois Irm?os e no 57 S?tio Santa M?nica, respectivamente. Em geral, nas duas propriedades, todas as cultivares avaliadas apresentaram bom desempenho para os diferentes tipos de manejo, com rendimento m?dio acima de 2 000 kg ha-1. A t?cnica de inocula??o das sementes com riz?bio foi bem avaliada pelos agricultores, pela facilidade de execu??o e pelo baixo custo de implanta??o.