This work deals with the development and possible application of natural law in the Czech judiciary. The first part of this work deals with relationship between natural and positive law, historical development of natural law and legal positivism. The second part investigates the current legal order of the Czech Republic and the practice of Constitutional Court, which should give judgements that are conformable with natural-law core of Constitution of the Czech Republic. The work reflects that there is some difficulty in application of natural rights in practice of Constitutional Court. On that account the work solves how is the natural law applied. However, the work finds out that there obviously aren?t any sources which would be treated about mentioned application. The third part of this work is engaged in possible proposal of natural law as a potential solution of difficulties that are connected with practice of Constitutional Court.