Thesis (Ph. D.)--University of Queensland, 2002. / Includes bibliographical references.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv. / Härtill 4 uppsatser.

Li Chi Ho. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2004. / Includes bibliographical references (leaves 137-162). / Abstracts in English and Chinese. / Statement --- p.iii / Acknowledgments --- p.iv / Abstract --- p.vi / Abstract (Chinese version) --- p.viii / Abbreviations --- p.x / Table of contents --- p.xii / List of Figure --- p.xviii / List of Table --- p.xx / Chapter Chapter One --- Literature Review / Chapter 1.1 --- The Genus Schistosoma --- p.1 / Chapter 1.2 --- Biology of Schistosoma japonicum --- p.3 / Chapter 1.2.1 --- The History of discovery of Schitosoma japonicum --- p.3 / Chapter 1.2.2 --- Life cycle of Schistosoma japonicum --- p.3 / Chapter 1.2.2.1 --- Egg --- p.6 / Chapter 1.2.2.2 --- Miracidia --- p.6 / Chapter 1.2.2.3 --- Sporocysts --- p.7 / Chapter 1.2.2.4 --- Cercaria --- p.9 / Chapter 1.2.2.5 --- Schislosomula --- p.10 / Chapter 1.2.2.6 --- Adult worms --- p.10 / Chapter 1.2.3 --- Genetics of Schistosoma japonicum --- p.11 / Chapter 1.2.3.1 --- Genome analysis --- p.12 / Chapter 1.2.3.2 --- Schistosome genome --- p.13 / Chapter 1.2.4 --- Tegumental membrane of Schistosomes --- p.14 / Chapter 1.3 --- Pathology of Schistosomiasis --- p.15 / Chapter 1.3.1 --- Acute Schistosomiasis --- p.15 / Chapter 1.3.2 --- Intestinal Disease --- p.16 / Chapter 1.3.3 --- Hepatosplenic Disease --- p.16 / Chapter 1.3.4 --- Cerebral Schistosomiasis --- p.16 / Chapter 1.4 --- Treatment of Schistosomiasis --- p.17 / Chapter 1.4.1 --- Chemotherapy --- p.17 / Chapter 1.4.2 --- Schistosoma Vaccine --- p.17 / Chapter 1.5 --- GPI anchor --- p.19 / Chapter 1.5.1 --- Function of GPI anchored proteins --- p.19 / Chapter 1.5.2 --- Synthesis of GPI anchor --- p.21 / Chapter 1.5.3 --- "Phosphatidylinositol Glycan, Class N (PIG-N)" --- p.26 / Chapter 1.6 --- The role of GPI anchor proteins in Schistosome --- p.26 / Chapter 1.7 --- Aim of study --- p.29 / Chapter Chapter two --- Materials and Methods / Chapter 2.1 --- Materials --- p.31 / Chapter 2.1.1 --- Cell lines and Bacterial Strains --- p.31 / Chapter 2.1.2 --- Chemicals --- p.32 / Chapter 2.1.3 --- Kits and Reagents --- p.34 / Chapter 2.1.4 --- Nucleic acids --- p.34 / Chapter 2.1.5 --- Reagents for Cell culture --- p.34 / Chapter 2.1.6 --- Solutions --- p.35 / Chapter 2.1.7 --- Enzymes --- p.37 / Chapter 2.1.8 --- Primer List --- p.37 / Chapter 2.1.9 --- Antibodies --- p.39 / Chapter 2.2 --- Methods / Chapter 2.2.1 --- Screening of the S. japonicum cercaria stage cDNA library --- p.40 / Chapter 2.2.1.1 --- λ phage plating --- p.40 / Chapter 2.2.1.2 --- Single plaque isolation --- p.40 / Chapter 2.2.1.3 --- Conversion of Lambda TriplEx to pTriplEx --- p.41 / Chapter 2.2.1.4 --- preparation of plasmid DNA --- p.41 / Chapter 2.2.1.5 --- cycle DNA sequencing --- p.42 / Chapter 2.2.2. --- RT-PCR --- p.44 / Chapter 2.2.2.1 --- Isolation of Total RNA by Guandidium Thiocyanate - Cesium Chloride ultracentrifugation --- p.44 / Chapter 2.2.2.2 --- Synthesis of Fist Strand cDNA by reverse transcriptation reaction --- p.45 / Chapter 2.2.2.3 --- PGR amplification of RT product --- p.46 / Chapter 2.2.3 --- Rapid Amplification of cDNA Ends (RACE) --- p.46 / Chapter 2.2.3.1 --- Synthesis of first strand cDNA for RACE reaction --- p.46 / Chapter 2.2.3.2 --- 5 ´ةRACE for Sj-PIG-N gene --- p.47 / Chapter 2.2.3.3 --- 3´ة RACE for Sj-PIG-N gene --- p.48 / Chapter 2.2.3.4 --- Purification of DNA fragment from agarose gel --- p.48 / Chapter 2.2.3.5 --- Ligation of purified PCR fragments and pBluescriptII KS(+) T-vector --- p.49 / Chapter 2.2.3.6 --- Preparation of DH5a competent cells --- p.49 / Chapter 2.2.3.7 --- Transformation of recombinant plasmid --- p.50 / Chapter 2.2.4 --- Mammalian cell transfection --- p.50 / Chapter 2.2.4.1 --- Stable transfection --- p.50 / Chapter 2.2.4.2 --- Transient transfection --- p.51 / Chapter 2.2.5 --- Biological function studies of Sj-PIG-N gene --- p.52 / Chapter 2.2.5.1 --- Flow Cytometry (FCM) analysis --- p.52 / Chapter 2.2.5.2 --- In Vitro Mannose Labeling of Microsomes and Characterization of Glycolipids --- p.53 / Chapter 2.2.6 --- Intracellular localization assay --- p.54 / Chapter Chapter Three --- Results / Chapter 3.1 --- Random sequence analysis of cercaria EST from S. japonicum Cercaria stage cDNA library --- p.55 / Chapter 3.1.1 --- Sequencing results --- p.57 / Chapter 3.1.2 --- BlastX Search results --- p.61 / Chapter 3.2 --- The expression of Sj-PIG-N gene in both adult worms and cercaria --- p.68 / Chapter 3.2.1 --- Spectrophotometric analysis of total RNA --- p.68 / Chapter 3.2.2 --- Detection of Sj-PIG-N gene expression in both adult worm and cercaria stages --- p.70 / Chapter 3.3 --- Cloning of the full length of Sj-PIG-N gene --- p.72 / Chapter 3.3.1 --- Amplification of the full length of Sj-PIG-N gene --- p.72 / Chapter 3.3.2 --- Obtaining the full length sequence of Sj-PIG-N gene --- p.74 / Chapter 3.4 --- Sequence analysis of Ml length Sj-PIG-N cDNA --- p.80 / Chapter 3.5 --- Analysis of Sj-PIG-N protein structure by computer programs --- p.91 / Chapter 3.6 --- Construction of Sj-PIG-N gene into mammalian cell expression vector pEGFP-Nl and pEGFP-Hyg --- p.97 / Chapter 3.6.1 --- Construction of pBluescriptII KS(+)-Sj-PIG-N-B --- p.97 / Chapter 3.6.2 --- Construction of pBluescriptll KS(+)-Sj-PIG-N-E --- p.101 / Chapter 3.6.3 --- Construction of pTRE2-Sj-PIG-N --- p.106 / Chapter 3.6.4 --- Construction of pEGFP-Hyg-Sj-PIG-N --- p.109 / Chapter 3.6.4.1 --- Construction of pEGFP-Hyg --- p.109 / Chapter 3.6.4.2 --- Construction of pEGFP-Hyg-Sj-PIG-N --- p.114 / Chapter 3.7 --- Molecular analysis of Sj-PIG-N gene --- p.117 / Chapter 3.7.1 --- Functional analysis of Sj-PIG-N --- p.117 / Chapter 3.7.1.1 --- FACS analysis of surface expression of GPI-anchored protein - Thyl --- p.117 / Chapter 3.7.1.2 --- FACS analysis of surface expression of GPI-anchor protein - CD24 --- p.119 / Chapter 3.7.1.3 --- In Vitro Mannose Labeling of Microsomes and Characterization of Glycolipids --- p.121 / Chapter 3.7.2 --- Localization of Sj-PIG-N with immunofluorescent staining --- p.123 / Chapter Chapter Four --- Discussion / Chapter 4.1 --- S. japonicum cercaria EST analysis --- p.125 / Chapter 4.2 --- Structure analysis of Sj-Pig-N gene --- p.128 / Chapter 4.3 --- Molecular analysis of Sj-PIG-N --- p.131 / Chapter 4.4 --- Further study --- p.134 / Chapter 4.5 --- Conclusion --- p.136 / References --- p.137

Thesis (Ph. D.)--University of Wisconsin--Madison, 1935. / Typescript. Vita. With this is bound: The morphology and anatomy of Lygodium japonicum / Hernert M. Clarke. Reprinted from American journal of botany, vol. 23, no. 6 (June 1936), P. [405]-413. Includes bibliographical references (leaves 47-48).

Thesis (M. Ag. Sc.)--University of Adelaide, Dept. of Agricultural Biochemistry, 1988. / Includes bibliographical references (leaves 109-119).

Thesis (Ph. D.)--University of Florida, 1985. / Description based on print version record. Typescript. Vita. Includes bibliographical references (leaves 137-150).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1965. / Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Submitted by Marco Antônio de Ramos Chagas (mchagas@ufv.br) on 2016-06-16T08:35:27Z No. of bitstreams: 1 texto completo.pdf: 711914 bytes, checksum: 511f604fff5df6ba29e27254ccaf0db2 (MD5) / Made available in DSpace on 2016-06-16T08:35:27Z (GMT). No. of bitstreams: 1 texto completo.pdf: 711914 bytes, checksum: 511f604fff5df6ba29e27254ccaf0db2 (MD5) Previous issue date: 1999-02-26 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Foram conduzidos quatro experimentos em condições de casa de vegetação, na Universidade Federal de Viçosa, objetivando determinar a influência do nematóide de cisto, Heterodera glycines Ichinohe, e do Bradyrhizobium japonicum sobre os teores de nutrientes em folhas da soja, bem como avaliar o efeito do nematóide, do nitrogênio e do Bradyrhizobium japonicum sobre características agronômicas da soja; e cinco experimentos em condições de campo, em Capinópolis e Rio Paranaíba, Minas Gerais, visando avaliar os danos causados por percevejos em sementes dessa leguminosa. Nos experimentos conduzidos em casa de vegetação, o delineamento foi de blocos casualizados, num esquema fatorial 4 4, com quatro repetições. No primeiro experimento, os tratamentos foram constituídos pela combinação dos fatores nematóide de cisto: 0, 3.000, 6.000 e 9.000 ovos de NCS/vaso e 45, 90, 180 e 270 mg N/dm 3 de solo. O nematóide de cisto aumentou significativamente os teores de K nas folhas, e o nitrogênio influenciou os teores de P, Ca e Fe na floração. No segundo experimento, os tratamentos foram constituídos pela combinação dos fatores nematóide de cisto: 0, 3.000, 6.000 e 9.000 ovos de NCS/vaso e 0; 0,4; 0,8; e 1,6 g de inoculante de Bradyrhizobium japonicum/100 sementes. O nematóide de cisto influenciou os teores de N, Ca, Mg, Mn, Zn, Cu, Fe e B nas folhas durante a floração. No terceiro experimento, os tratamentos foram constituídos pela combinação dos fatores nematóide de cisto: 0, 3.000, 6.000 e 9.000 ovos de NCS/vaso e 45, 90, 180 e 270 mg N/dm 3 de solo. A altura de planta e o peso de matéria seca da parte aérea aumentaram (P < 0,05) com as doses crescentes de N, e estas reduziram a nodulação. No quarto experimento, os tratamentos foram constituídos pela combinação dos fatores nematóide de cisto: 0, 3.000, 6.000 e 9.000 ovos de NCS/vaso e 0; 0,4; 0,8; e 1,6 g de inoculante de Bradyrhizobium japonicum/100 sementes. A altura de planta, o número de vagens por planta de nódulos e o peso de semente e de matéria seca da parte aérea e da raiz diminuíram (P < 0,05) com as doses crescentes de nematóide de cisto. Nos vasos inoculados com 3.000 e 6.000 ovos de NCS, o número de fêmeas e de cistos foi superior (P < 0,05) ao encontrado nas demais combinações de tratamentos. A presença do nematóide, independentemente da concentração de ovos, resultou numa redução significativa das características avaliadas. As doses crescentes do inoculante de Bradyrhizobium japonicum aumentaram (P < 0,05) os valores das características avaliadas. No quinto e sexto experimentos, conduzidos em Capinópolis, as 20 linhagens/variedades de soja foram avaliadas em duas épocas de colheita, correspondentes às respectivas épocas de semeadura. Em Rio Paranaíba, instalaram-se outros três experimentos, e as 20 linhagens/variedades de soja foram avaliadas em três épocas de colheita, também correspondentes às épocas de semeadura. Nestas épocas, além do peso da semente e dos danos causados por percevejos, observaram-se a intensidade desses danos e a sua localização na semente. A linhagem UFV96-37001 e as variedades CAC-1, Doko RC e UFV-18, independentemente da época de colheita, não diferiram (P > 0,05) das outras linhagens nas características avaliadas, indicando que o cultivo de linhagens com ausência de enzimas lipoxigenases não foi afetado diferentemente pelos percevejos. / Four experiments were carried out under greenhouse conditions at the Universidade Federal de Viçosa (UFV), with the purpose of determining the influence of cysts nematode, Heterodera glycines Ichinohe, of Bradyrhizobium japonicum on nutrient contents in soybean leafs; evaluate the effects of cysts nematode, of nitrogen fertilization and Bradyrhizobium japonicum on agricultural characteristics on soybean plants, and five experiments on field conditions of Capinópolis and Rio Paranaíba, Minas Gerais, Brazil, with de purpose to evaluate the damages caused by stink bugs on soybean seeds. At the study carried out under greenhouse, a factorial experimental design was used to the treatments. At first experimental design, the inoculum concentrations were 0, 3,000, 6,000 and 9,000 eggs per clay pot containing a single plant, and nitrogen fertilization was applied at 45, 90, 180 and 270 mg N/dm 3 of soil. The cysts nematode influenced leaf the contents of K and nitrogen fertilizer influenced the leaf contents of P, Ca and Fe, at flowering. At second experimental design, the inoculum concentrations were 0, 3,000, 6,000 and 9,000 eggs per clay pot containing a single plant and Bradyrhizobium japonicum inoculum concentrations were 0; 0,4; 0,8 and 1,6 g/100 seeds. The leaf contents of N, Ca, Mg, Mn, Zn, Fe and B were influenced by cysts nematode. At third experimental design, the inoculum concentrations were 0, 3,000, 6,000 and 9,000 eggs per clay pot containing a single plant, and nitrogen fertilization was applied at 45, 90, 180 and 270 mg N/dm 3 . The effects on soybean were estimated at the end of the growth cycle. Plant height and weight of the dried aerial plant parts increased linearly (P < 0,005) as the nitrogen levels also increased. At fourth experimental design, the inoculum concentrations were 0, 3,000, 6,000 and 9,000 eggs per clay pot containing a single plant and Bradyrhizobium japonicum inoculum concentrations were 0; 0,4; 0,8 and 1,6 g/100 seeds. The number of cysts in pots inoculated with 3,000 and 6,000 eggs was statistically higher (P < 0,05) than found in the remaining treatments. Regardless of egg concentration used in the inoculation, nematode presence resulted in a significant reduction in soybean characteristics and the nodulation was reduced by the nitrogen fertilization. At fifth and sixth experimental designs, the 20 soybean lines/varieties was evaluated in two periods of harvest. Tree another experimental designs were carried out at Rio Paranaíba, using 20 also soybean lines varieties that were evaluated in three periods of harvest. Besides weight of seeds and damage, the intensity of damages and the localization were observed. The stink bugs of seeds showed preference to feed on large seeds. The line UFV 96-37001 and the varieties CAC-1, Doko RC and UFV-18, independently of the period of harvest, there were no (P > 0,05) difference among lines with other lines on evaluated characteristics, so it showing that the cultivation of absence lines of lipoxygenases it is no affected by stink bugs. / Tese antiga, sem ficha catalog.

Law Pui-ki. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 98-105). / Abstracts in English and Chinese. / Statement --- p.i / Acknowledgments --- p.ii / Abstract --- p.iii / Table of contents --- p.viii / List of figures --- p.xv / List of tables --- p.xvii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Schistosomiasis --- p.1 / Chapter 1.1.1 --- Disease burden --- p.1 / Chapter 1.1.2 --- Causative agents --- p.1 / Chapter 1.1.3 --- Transmission --- p.2 / Chapter 1.1.4 --- Pathology of the disease --- p.3 / Chapter 1.1.5 --- Control and therapy --- p.3 / Chapter 1.2 --- Schistosomiasis in China --- p.5 / Chapter 1.3 --- Schistosoma japonicum --- p.6 / Chapter 1.3.1 --- Life cycle of S. japonicum --- p.6 / Chapter 1.3.2 --- Biology of S. japonicum cercaria --- p.9 / Chapter 1.3.3 --- Transformation of cercaria to schistosomulum --- p.10 / Chapter 1.3.4 --- Cercarial stage-specific antigens --- p.12 / Chapter 1.4 --- Aim of study --- p.14 / Chapter Chapter 2 --- Materials and methodology --- p.15 / Chapter 2.1 --- Materials --- p.15 / Chapter 2.1.1 --- Schistosoma japonicum cercaria cDNA library --- p.15 / Chapter 2.1.2 --- Cercarial stage-specific clones --- p.15 / Chapter 2.1.3 --- Adult worm and cercaria RNA --- p.18 / Chapter 2.1.4 --- "Snail intermediate host, Oncomelania hupensis" --- p.18 / Chapter 2.1.5 --- Bacterial strains --- p.18 / Chapter 2.1.6 --- Chemicals --- p.19 / Chapter 2.1.7 --- Kits and reagents --- p.21 / Chapter 2.1.8 --- Nucleic acids --- p.22 / Chapter 2.1.9 --- Solutions --- p.22 / Chapter 2.1.10 --- Enzymes --- p.24 / Chapter 2.1.11 --- Primers --- p.25 / Chapter 2.1.12 --- Antibodies --- p.26 / Chapter 2.2 --- Methodology --- p.27 / Chapter 2.2.1 --- Identification of cercarial stage-specific genes --- p.27 / Chapter 2.2.1.1 --- Sequence analysis of cercarial stage-specific clones --- p.27 / Chapter 2.2.1.2 --- "Confirmation of stage-specific expression of the selected gene, 20H8 and sjCa8, by RNA dot blot" --- p.29 / Chapter 2.2.1.2.1 --- Cloning of S. japonicum glyceraldehyde-3-phosphate dehydrogenase (sjGAPDH) (GenBank accession no. U75571) --- p.29 / Chapter 2.2.1.2.1.1 --- Reverse transcription and PCR amplification of sjGAPDH --- p.29 / Chapter 2.2.1.2.1.2 --- Cloning of sjGADPH in pBluescript II KS (-) --- p.29 / Chapter 2.2.1.2.1.3 --- Sequence verification of cloned sjGAPDH --- p.30 / Chapter 2.2.1.2.2 --- Synthesis of Digoxigenin (DIG)-labeled probe --- p.31 / Chapter 2.2.1.2.2.1 --- Synthesis of DIG-labeled probe by PCR --- p.31 / Chapter 2.2.1.2.2.2 --- Estimation of concentration of DIG-labeled probe --- p.32 / Chapter 2.2.1.2.3 --- RNA dot blot --- p.33 / Chapter 2.2.1.2.3.1 --- Transferring RNA to the membrane using the BIO-RAD blotting manifold --- p.33 / Chapter 2.2.1.2.3.2 --- Hybridization of DIG-labeled probe to detect sjGAPDH --- p.33 / Chapter 2.2.1.2.3.3 --- Detection of the chemiluminescent signal --- p.33 / Chapter 2.2.1.2.3.4 --- Stripping membrane for reprobing --- p.34 / Chapter 2.2.2 --- Characterization of the cercarial stage-specific gene and gene product of 20H8 --- p.35 / Chapter 2.2.2.1 --- Cloning of full-length cDNA of 20H8 --- p.35 / Chapter 2.2.2.1.1 --- 5'RACE of 20H8 --- p.35 / Chapter 2.2.2.1.2 --- Cloning of full-length 20H8 into pBluescript II SK(-) --- p.36 / Chapter 2.2.2.2 --- Analysis of DNA sequence and deduced amino acid sequence of 20H8 --- p.37 / Chapter 2.2.2.3 --- Demonstration of the immunogenicity and antigenicity of 20H8 --- p.38 / Chapter 2.2.2.3.1 --- Expression of 20H8 in E. coli --- p.38 / Chapter 2.2.2.3.1.1 --- "Cloning of 20H8 in an E coli expression vector, pET32a+" --- p.38 / Chapter 2.2.2.3.1.2 --- Expression of recombinant 20H8 protein in E. coli --- p.39 / Chapter 2.2.2.3.2 --- Purification and concentration of recombinant 20H8 protein --- p.40 / Chapter 2.2.2.3.3 --- Production of antiserum --- p.40 / Chapter 2.2.2.3.4 --- Evaluation of immunogenicity of recombinant 20H8 protein --- p.41 / Chapter 2.2.2.3.5 --- Evaluation of antigenicity of recombinant 20H8 protein --- p.42 / Chapter 2.2.2.4 --- Immunolocalization of 20H8 in cercaria --- p.43 / Chapter 2.2.2.4.1 --- Collection of S. japonicum cercaria --- p.43 / Chapter 2.2.2.4.2 --- Immunofluorescence staining of cercaria --- p.43 / Chapter 2.2.3 --- Characterization of the cercarial stage-specific gene and gene product of sjCa8 --- p.45 / Chapter 2.2.3.1 --- Analysis of DNA sequence and deduced amino acid sequence of sjCa8 --- p.45 / Chapter 2.2.3.2 --- Demonstration of the immunogenicity and antigenicity of sjCa8 --- p.46 / Chapter 2.2.3.2.1 --- Expression of sjCa8 in E. coli --- p.46 / Chapter 2.2.3.2.2 --- Purification and concentration of recombinant sjCa8 protein --- p.46 / Chapter 2.2.3.2.3 --- Production of antiserum --- p.46 / Chapter 2.2.3.2.4 --- Evaluation of the immunogenicity of sjCa8 --- p.47 / Chapter 2.2.3.2.5 --- Evaluation of the antigenicity of sjCa8 --- p.47 / Chapter 2.2.3.3 --- Demonstration of calcium-binding property of sjCa8 --- p.48 / Chapter 2.2.3.3.1 --- Calcium-dependent electrophoretic mobility shift --- p.48 / Chapter 2.2.3.3.2 --- Ruthenium red assay --- p.48 / Chapter 2.2.3.4 --- Immunolocalization of sjCa8 in cercaria --- p.49 / Chapter Chapter 3 --- Results --- p.50 / Chapter 3.1. --- Identification of cercarial stage-specific genes --- p.50 / Chapter 3.1.1 --- Identification of cercarial stage-specific genes by microarray --- p.50 / Chapter 3.1.2 --- "Confirmation of stage-specific expression of the selected gene, 20H8 and sjCa8, by RNA dot blot" --- p.54 / Chapter 3.2. --- Characterization of the cercarial stage-specific gene and gene product of 20H8 --- p.57 / Chapter 3.2.1 --- Cloning of full-length cDNA of 20H8 --- p.57 / Chapter 3.2.2 --- Analysis of DNA sequence and deduced amino acid sequence of 20H8 --- p.59 / Chapter 3.2.3 --- Demonstration of the immunogenicity and antigenicity of 20H8 --- p.62 / Chapter 3.2.3.1 --- Expression of 20H8 in E. coli --- p.62 / Chapter 3.2.3.2 --- Purification and concentration of recombinant 20H8 protein --- p.64 / Chapter 3.2.3.3 --- Production of antiserum --- p.65 / Chapter 3.2.3.4 --- Evaluation of immunogenicity of recombinant 20H8 protein --- p.66 / Chapter 3.2.3.5 --- Evaluation of antigenicity of recombinant 20H8 protein --- p.67 / Chapter 3.2.4 --- Immunolocalization of 20H8 in cercaria --- p.68 / Chapter 3.3. --- Characterization of the cercarial stage-specific gene and gene product of sjCa8 --- p.70 / Chapter 3.3.1 --- Analysis of DNA sequence and deduced amino acid sequence of sjCa8 --- p.70 / Chapter 3.3.2 --- Demonstration of the immunogenicity and antigenicity of sjCa8 --- p.75 / Chapter 3.3.2.1 --- Expression of sjCa8 in E. coli --- p.75 / Chapter 3.3.2.2 --- Purification and concentration of recombinant sjCa8 protein --- p.76 / Chapter 3.3.2.3 --- Production of anti-sjCa8 serum --- p.77 / Chapter 3.3.2.4 --- Evaluation of immunogenicity of sjCa8 protein --- p.77 / Chapter 3.3.2.5 --- Evaluation of antigenicity of sjCa8 protein --- p.78 / Chapter 3.3.3 --- Demonstration of calcium-binding property of sjCa8 --- p.79 / Chapter 3.3.3.1 --- Electrophoretic motility shift --- p.80 / Chapter 3.3.3.2 --- Ruthenium red binding assay --- p.80 / Chapter 3.3.4 --- Immunolocalization of sjCa8 in cercaria 81 --- p.81 / Chapter Chapter 4 --- Discussion --- p.83 / Chapter 4.1 --- Identification of cercarial stage-specific genes --- p.83 / Chapter 4.1.1 --- Identification of cercarial stage-specific genes by microarray --- p.83 / Chapter 4.1.2 --- "Confirmation of stage-specific expression of the selected genes, 20H8 and sjCa8" --- p.85 / Chapter 4.2 --- Characterization of the cercarial stage-specific gene and gene product of 20H8 --- p.86 / Chapter 4.2.2 --- Analysis of DNA sequence and deduced amino acid sequence of 20H8 --- p.86 / Chapter 4.2.3 --- Demonstration of the immunogenicity and antigenicity of 20H8 --- p.88 / Chapter 4.2.4 --- Immunolocalization of 20H8 in cercaria --- p.90 / Chapter 4.3 --- Characterization of the cercarial stage-specific gene and gene product of sjCa8 --- p.91 / Chapter 4.3.1 --- Analysis of DNA sequence and deduced amino acid sequence of sjCa8 --- p.91 / Chapter 4.3.2 --- Demonstration of the immunogenicity and antigenicity of sjCa8 --- p.93 / Chapter 4.3.3 --- Demonstration of calcium-binding property of sjCa8 --- p.94 / Chapter 4.3.4 --- Immunolocalization of sjCa8 in cercaria --- p.94 / Chapter 4.4 --- Conclusions --- p.95 / References --- p.96

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