Zugl.: Giessen, Universiẗat, Diss., 2008.

Thesis (doctoral)--Lund University, 1992. / Added t.p. with thesis statement inserted.

Thesis (Ph. D.)--Rutgers University, 2008. / "Graduate Program in Electrical and Computer Engineering." Includes bibliographical references (p. 80-83).

Thesis (Ph. D.)--University of Virginia, 2000. / Spine title: Synaptic role of TYR-phosphatases. Includes bibliographical references (p. 135-172). Also available online through Digital Dissertations.

Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2005. / Includes bibliographical references (leaves 151-171). Also available in electronic version.

Thesis (doctoral)--Lund University, 1992. / Added t.p. with thesis statement inserted.

Universiẗat, Diss., 2001--Bonn.

Thesis (Ph.D.)--York University, 2005. Graduate Programme in Biology. / Typescript. Includes bibliographical references (leaves 166-200). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://wwwlib.umi.com/cr/yorku/fullcit?pNR11583

Synapse development is a stepwise process that requires the recruitment of key synaptic components to active zones, followed by continual maintenance of these structures to maintain connectivity and stability throughout the life of the organisms. Early synapse development requires the recruitment of early scaffolding proteins to establish stable connectivity as well as provide sites of recruitment of other vital synaptic proteins. One of the earliest proteins to be localized to the synapse is the conserved protein Syd-1. Syd-1 proteins contain a Rho GTPase activating protein (GAP)-like domain of unclear significance. Here I show that Drosophila Syd-1 interacts with all six fly Rhos and has GAP activity towards RAC1. I then show that lacks GAP activity localizes normally to presynaptic sites and is sufficient to recruit Nrx-1 but fails to cluster Brp normally and genetically interacts with RAC1 in vivo. I conclude that contrary to previous models, the GAP domain of fly Syd-1 is active and required for presynaptic development. Additionally, I’ve identified a previously uncharacterized protein, Vezl, as being critical for retrograde axonal transport and synaptic maintenance. I found that Vezl required for normal neuronal growth and that vezl loss resulted in decreased neuron size and the formation of swollen neuronal terminals that accumulated membrane markers and axonal transport cargo. I found that vezl mutants specifically retrograde transport of cargo and particularly affected signaling endosomes. The signaling endosomes were unable to initiate retrograde transport in vezl mutants and remained stuck within the distal boutons unable to relay their signaling peptides back to the nucleus. I conclude that Vezl is serving a role in attaching retrograde cargo to dynein and the microtubules specifically at neuron tips so that they can undergo retrograde axonal transport. This dissertation includes previously published and unpublished co-authored material. / 2020-09-06

Made available in DSpace on 2014-06-11T19:24:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-07-19Bitstream added on 2014-06-13T19:52:09Z : No. of bitstreams: 1 esberard_rr_me_arafo.pdf: 2831212 bytes, checksum: 853c7064958304ce94ed88b7ba2ae73f (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo desse estudo foi avaliar, em microscopia eletrônica de varredura, a morfologia do esmalte, da dentina, do cemento e, principalmente, da junção amelocementária humanos após o processo de clareação dentária, comparando-se a ação agressiva das diferentes técnicas de clareação e dos agentes clareadores sobre os tecidos que compõem a junção amelocementária. Quarenta e cinco dentes foram selecionados e seccionados ao meio, no sentido vestíbulo-lingual, de modo que obteve-se noventa espécimes. Assim, quarenta e cinco espécimes serviram de controle e seus pares foram submetidos às seguintes técnicas de clareação: Grupo I- clareação externa com peróxido de carbamida 10% (Opalescence); Grupo II- clareação externa com peróxido de hidrogênio 35% (Lase Peroxide); Grupo III- clareação externa com peróxido de hidrogênio 35% (Opalescence Xtra); Grupo IV- clareação interna/externa com peróxido de hidrogênio 35% (Lase Peroxide); Grupo V- clareação interna/externa com peróxido de hidrogênio 35% (Opalescence Xtra) e Grupo VI- clareação interna com pasta de perborato de sódio + peróxido de hidrogênio 30 V. Após as clareações, todos os espécimes, clareados ou não, foram avaliados em MEV. Os espécimes dos Grupos I, II e III foram avaliados externamente e os espécimes dos Grupos IV, V e VI foram avaliados interna e externamente. A análise microscópica mostrou que ocorreram alterações no esmalte, no cemento e na dentina de todos os espécimes clareados, porém, a junção amelocementária foi a parte mais afetada pelos agentes clareadores estudados, os quais promoveram mudanças no padrão da junção, aumentado a exposição da superfície dentinária e formando junções do tipo gaps ou fenestradas, exibindo extensas áreas de cemento separado do esmalte, sem cemento intermediário e com exposição dos túbulos dentinários. Aplicou-se o... . / The purpose of this study was to evaluate in SEM the effects of bleaching agents on the surface of human enamel, dentin, cementum and cementoenamal junction, comparing the action of the different techniques and bleaching agents used. Forty five teeth were selected and sectioned longitudinally so ninety specimens were obtained. Forty five specimens served as controls and the other ones were bleached by sixs protocols: Group I- external bleaching with 10% carbamide peroxide (Opalescence); Group II- external bleaching with 35% hydrogen peroxide (Lase Peroxide); Group III- external bleaching with 35% hydrogen peroxide (Opalescence Xtra); Group IV- internal/external bleaching with 35% hydrogen peroxide (Lase Peroxide); Group V- internal/external bleaching with 35% hydrogen peroxide (Opalescence Xtra) and Group VI- intracoronal bleaching with paste of sodium perborate mixed with 9% hydrogen peroxide. All the specimens were examined in SEM. The specimens of the Groups I, II and III were analised externally and the specimens of the Groups IV, V and VI were analised internal and externally. The SEM analysis showed some changes on the morphology of enamel, dentin and cementum of all bleached specimens, but the cementoenamel junction were the most affected part of the teeth. The bleaching agents used in this study caused alterations on the shapes of the cementoenamel junction increasing the exposition of dentin in this area and separating the enamel and the cementum. Kruskal-Wallis test was applied (H=6,947). The Groups I, III, V and VI showed similar results but the Groups II and IV showed identical behavior, more agressive than the others, but not statiscally significant.