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Juvenile hormone signaling in insect development and reproduction / Juvenile hormone signaling in insect development and reproductionSMÝKAL, Vlastimil January 2014 (has links)
This thesis comprises three published papers and one manuscript, all focused on the role of juvenile hormone (JH), the JH receptor Methoprene-tolerant (Met) and its target gene Krüppel-homolog 1 (Kr-h1) in insect development and reproduction. The JH-Met-Kr-h1 pathway is critical for metamorphic transition in hemimetabolan Pyrrhocoris apterus (Hemiptera) and holometabolan Bombyx mori (Lepidoptera) but seems to be dispensable during early larval postembryonic development. The results also show that Met and its heterodimeric partner Taiman (Tai) but not Kr-h1 are critical for ovarian development and vitellogenesis in Pyrrhocoris females. In vitro, in vivo and cell-based techniques in Drosophila melanogaster have demonstrated that Met and its paralog Gce are a bona fide receptor for JH. Only Gce capable of binding JH rescued Drosophila deficient for Met and Gce proteins, and the capacity of Gce to bind JH was necessary for JH-dependent transcriptional activation by Gce and Tai.
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ホソヘリカメムシにおけるKrüppel homolog 1 遺伝子の発現と機能に関する研究 / Expression and function of Krüppel homolog 1 gene in Riptortu pedestris董, 笠 23 March 2021 (has links)
京都大学 / 新制・課程博士 / 博士(理学) / 甲第23044号 / 理博第4721号 / 新制||理||1677(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)教授 沼田 英治, 教授 曽田 貞滋, 准教授 森 哲 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM
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Elucidating the Function of Krüppel Homolog 1 (Kr-h1) Associated Proteins (KAPs) in Aedes aegypti Reproduction Through RNA Interference-Mediated DownregulationZhang, Liyan 15 July 2024 (has links)
The transcription factor Krüppel homolog 1 (Kr-h1) is crucial in multiple reproductive processes of Aedes aegypti mosquitoes, including previtellogenesis, vitellogenesis, and oogenesis. This study explores the interaction between Kr-h1 and its potential associated proteins (KAPs), with a specific focus on the dimerization partner (DP-1), and how this interaction regulates gene expression pathways critical for mosquito reproduction. Utilizing RNA interference (RNAi), the research identifies DP-1 as a significant regulator of follicle growth post-eclosion (PE), highlighting its vital role in the mosquito reproductive regulatory pathway. The experimental approach included RNAi-mediated knockdown of DP-1, accompanied by evaluations using quantitative PCR (qPCR), Western blotting (WB), co-immunoprecipitation (Co-IP), follicle length measurement, and egg counting to assess the role of DP-1 in reproductive functions. For the first time, the inhibition of DP-1 expression was found to significantly impede A. aegypti follicular development. The elucidation of the mechanistic roles of Kr-h1 and DP-1 provides valuable insights that could lead to innovative strategies for mosquito population control and effective disease vector management. / Master of Science in Life Sciences / Mosquitoes can spread serious insect-borne diseases such as dengue, Zika, and malaria, etc. These diseases can infect hundreds of millions of individual and cause around a million of death annually. This study focuses on a specific mosquito species, Aedes aegypti, which is a major carrier of these diseases. To manage their populations and reduce the spread of these diseases, scientists are constantly seeking new methods to control their reproduction. Chemical insecticides are one of the most efficient and widely used strategies. However, these insecticides face significant challenges, including the development of resistance in mosquito populations and the potential damage to non-target species to affect the ecosystem. To address this issue, the development of new insecticides is crucial. We can identify new targets to pave the way to research novel effective insecticides. Inside mosquitoes, there are various proteins that help control their ability to reproduce. One of these proteins is called Krüppel homolog 1 (Kr-h1). Kr-h1 plays a crucial role in the development and reproductive processes of mosquitoes. Our research looked at how Kr-h1 interacts with other types of protein to control mosquito reproduction. Through various experiments, including gene expression analysis and protein studies, we found that DP-1 is essential for the proper development of mosquito eggs. This insight helps us understand more about the biological processes and hormonal pathways during mosquito reproduction, therefore provide greater opportunity to develop insecticides to reduce their populations and the spread of the diseases they carry.
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