Epidemiology of head and neck cancers is currently intensively studied topic. Recent shift in the age of incidence towards younger population is generally attributed to growing proportion of head and neck cancers caused by human papillomavirus - sexually transmitted virus which causes asymptomatic, but sometimes persisting infection that can lead to malignant transformation of the infected tissue. Significance of the topic lies mainly in the prognostic ad- vantage of patients with virally induced head and cancers and preventabil- ity of infections with human papillomavirus. First aim of this thesis is to demonstrate epidemiological trends of head and neck cancers in the Czech Republic, with the focus on change of age-specific incidence and mortality in the last three decades. Second aim of this thesis is to identify risk factors of oral infections with human papillomaviruses and head and neck cancer in a case-control and cross-sectional study of a hospital-based cohort. 1

Colorectal cancer (CRC) is the third most common cancer worldwide; it is responsible for nearly 10% of all newly diagnosed cancers and is the second most cause of cancer related death in Europe. Biomarkers for therapy guidance, targeted therapy and survival prognosis are still limited. As CRC is a heterogeneous disease, different parts of the tumor might have varying molecular characteristics which may change during therapy or disease progression. Through solid biopsies and screenings, these local or temporal differences are impossible to monitor. To facilitate detection of these possible temporal changes, a regularly and non-invasively accessible biomarker is required for disease monitoring. Circulating tumor cells (CTCs) might represent such a biomarker as they have been shown to be fluid surrogates of the solid tumor. EpCAM positive CTCs have shown to be prognostic in CRC for survival, but their full potential has not yet been evaluated further. By using the High Definition Single Cell Analysis (HD-SCA) workflow, we were able to analyze the entire spectrum of CTCs and categorize them as the regular CTCs (HD-CTC), CTCs with a smaller nuclear area (CTC-Small), CTCs with low expression of epithelial marker cytokeratin (CTC-LowCK) and CTCs undergoing apoptosis and therefore releasing cell free DNA...

Colorectal cancer (CRC) is a major public health problem worldwide. Despite improvements in the diagnostic process and advancement in the treatment methods, the prognosis remains poor. To improve survival rates, it is important to identify people with the predisposition for CRC and to detect the potentially curable early stage of the disease. Furthermore, identifying those who would have an adverse clinical outcome associated with a particular chemotherapy would help to avoid redundant chemotherapy burden in patients and contribute to enhanced therapeutic efficacy, while minimizing treatment-related toxicity. The aim of the Thesis was to search for novel promising diagnostic, prognostic and predictive DNA-based biomarkers of sporadic form of CRC. As each patient is genetically unique, these biomarkers would aid clinicians in better diagnosis and/or in the selection of an optimal type of therapy for an individual CRC patient based on their molecular profile. In order to explore this issue, we investigated several candidate genes in healthy individuals as well as in newly diagnosed cancer patients. The major outcomes of this PhD study, which were fully reported in seven publications included in the present Thesis, are 1) The observation of several candidate single nucleotide polymorphisms in microRNA...

Master thesis "Czech Republic and its connection to new LNG terminals - strengthening of the gas security" examines the level of gas security in the Czech Republic. Dependency of the Czech Republic on gas supplies from foreign countries is almost 100 %. Russian federation is dominant supplier with roughly 75 % of the supplies. High dependency on one exporter is considered hazardous from energy security perspective. The Czech government should strive to change and improve this situation. The diversification is considered as a useful tool to improve the gas security. Currently there are new gas projects built in neighboring countries, which may influence energy security of the Czech Republic. The study researches the impact of these new projects on the Czech Republic. Namely we focused on gas pipeline Nord Stream and its Czech connection Gazelle, which will connect North Germany and Bavaria through the Czech Republic. Second project is gas network called "the North - South Corridor" connecting Baltic and Adriatic Sea. The North - South corridor may be potentially linked up to the new LNG terminals Świnoujście in Poland or Adria in Croatia. Furthermore, there are long-lasting plans to open new "South" gas route from Caspian region to Europe. The main goal of this thesis is to explore real...

Colorectal cancer (CRC) is the third most common cancer worldwide; it is responsible for nearly 10% of all newly diagnosed cancers and is the second most cause of cancer related death in Europe. Biomarkers for therapy guidance, targeted therapy and survival prognosis are still limited. As CRC is a heterogeneous disease, different parts of the tumor might have varying molecular characteristics which may change during therapy or disease progression. Through solid biopsies and screenings, these local or temporal differences are impossible to monitor. To facilitate detection of these possible temporal changes, a regularly and non-invasively accessible biomarker is required for disease monitoring. Circulating tumor cells (CTCs) might represent such a biomarker as they have been shown to be fluid surrogates of the solid tumor. EpCAM positive CTCs have shown to be prognostic in CRC for survival, but their full potential has not yet been evaluated further. By using the High Definition Single Cell Analysis (HD-SCA) workflow, we were able to analyze the entire spectrum of CTCs and categorize them as the regular CTCs (HD-CTC), CTCs with a smaller nuclear area (CTC-Small), CTCs with low expression of epithelial marker cytokeratin (CTC-LowCK) and CTCs undergoing apoptosis and therefore releasing cell free DNA...