High-school students are less interested in science then before. Despite importance of science for our life and for description of nature around us, students lose motivation for studying it. The approach of teacher is critical for focusing students' attention. Lessons should be based on linking of the education with daily-life and biology provides plenty of possibilities to achieve the goal, especially employing hand-on education, practical examples and exercises. One of the most effective way to show the beauty and a complexity of organism structure is by using microscopes. Many different techniques have been used to prepare education materials - picture, videos or whole movies from micro-word. Nevertheless, a classical optical microscopy is by far the most common training activity during the practical courses. An evaluation of this education aspects is, therefore, critical for further method development. This diploma thesis is focused on establishing of evaluation techniques and subsequent analysis of the current state of students' microscopy skills. First of all, the elementary set of knowledge and the correct microscopy work-flow were established in collaboration with eleven experienced teachers. Based on these results, two types of surveys were designed. One of them was dedicated to teachers...