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Leaf size and shape are strongly influenced by the growth patterns of the epidermal tissue. Pavement cells are the prevalent cell type in the epidermis and during cell expansion they undergo a drastic shape change from a simple polyhedral cells to puzzled-shaped cell. The role of these cell protrusions, more commonly referred to as lobes, remains unknown but their formation has been proposed to help increase the structural integrity of the epidermal tissue. How the symmetry breaking event that initiates a lobe is controlled remains unknown, however pharmacological and genetic disruption of the microtubule system has been shown to interfere not only with lobe initiation but also with lobe expansion. Additionally, the role of microtubules in the pattering of microfibril deposition, the load-bearing structure of the cell wall, makes the microtubule system a good candidate to evaluate its dynamics as a function of shape change. Two main mechanical models for lobe initiation are evaluated here, one where microtubules serve as stable features suppressing local expansion and one where microtubules, similarly to the anisotropic expansion patterning in hypocotyl cells, pro-mote the local anisotropic expansion of the cell resulting in lobe formation. The main method to evaluate these models was through the use of long-term time-lapse image analysis using a plasma-membrane marker for accurate shape change quantification and a microtubule marker to quantify their location, persistence, and density as a function of cell shape change. Using the junctions where three cells come together,cells were sub-divided into segments and the shape of these segments were tracked using a new coordinate system that allowed the detection of new lobes as which can arise from ∼300 deflections. By mapping sub-cellular processes, such as microtubule persistence, to this coordinate system, correlations of microtubule organization and shape change was possible. Additionally, a subset of microtubules bundles that splay across the anticlinal and periclinal walls, perpendicular and parallel to the leaf surface respectively, were identified as marking the location and direction of lobe formation.Disrupting the cell boundary by partially digesting pectin, a main component in the middle lamella, revealed the cell-autonomous morphogenesis mechanism in pavementcells. Under pectinase treatment, cell invaginations were produced and similarly to lobes their initiation was microtubule and cellulose dependent. Lastly, stress prediction using finite-element models, based from live-cell images, co-localized regions of high cell wall stress with both microtubule persistence and shape shape locations in both lobing and invaginated segments. Together, a model of cellular shape change is presented where microtubules translate cell wall stresses to tissue morphogenesis.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Growth dynamics of Italian ryegrass (Lolium multiflorum Lam) under different grazing intensities were studied, using marked tillers. The experimental design was completely randomized, with four repetitions (transects) and three treatments consisting of grazing intensities: High , Mean and Low , corresponding to the disappearance of 61.0, 43.3 and 21.1% of the initial forage mass value, respectively. The experimental animals were ewe lambs; rotational grazing was used and the thermal sum of 313 degree-days determined the interval among grazing periods. Grazing intensities affected the morphogenesis and structure of Italian ryegrass only in the period from 24/07 to 02/09. During this period, the Italian ryegrass in the Mean grazing intensity had the lowest leaf appearance rate and leaf elongation rate and it had the highest values of leaf life span. The pseudostem height, the length of the intact and defoliated blades, the number of intact and senescent leaves were higher in Low intensity, and did not differ between Mean and High . In Low intensity, the highest growth and senescence flows were registered, respectively, 85.6 and 54.6 kg/ha day of dry matter (DM). In Mean and High intensities, the growth and intake flows were higher in the beginning of pasture utilization and the senescence flow increased during the Italian ryegrass growing season. The choice of the grazing intensity should be based on the aim of management: when the intention is to prioritize individual weight gain, it is recommended the adoption of the intensity of 43.3%, which allows greater intake of blade s leaf by lambs; but when the objective is to prioritize production per area, it is recommended to use the grazing intensity of 61.0%, because under this intensity Italian ryegrass allows higher stocking rate values. / Estudou-se a dinâmica de crescimento de azevém (Lolium multiflorum Lam.) sob diferentes intensidades de pastejo, por meio de perfilhos marcados. O delineamento experimental foi inteiramente casualizado, com quatro repetições (transectas) e três tratamentos constituídos por intensidades de pastejo: Alta , Média , Baixa , correspondentes ao desaparecimento de 61,0; 43,3 e 21,1% do valor da massa de forragem inicial, respectivamente. Os animais experimentais foram cordeiras, o pastejo foi rotativo e a soma térmica de 313 graus-dia constituiu o intervalo entre pastejos. As intensidades de pastejo afetaram a morfogênese e estrutura de azevém apenas no período de 24/07 a 02/09. Nesse período, o azevém, na intensidade de pastejo Média , apresentou as menores taxas de aparecimento e elongação foliar e os maiores valores de duração de vida das folhas. A altura do pseudocolmo, o comprimento de lâminas intactas e desfolhadas, o número de folhas intactas e em senescência foram superiores na intensidade Baixa , sem diferir entre Média e Alta . Na intensidade Baixa observaram-se os maiores fluxos de crescimento e senescência, respectivamente 85,6 e 54,6 kg/ha/dia de matéria seca (MS). Nas intensidades Média e Alta , os fluxos de crescimento e consumo foram superiores no início da utilização da pastagem, enquanto o fluxo de senescência aumentou ao longo da estação de crescimento do azevém. A escolha da intensidade de pastejo deve ser baseada no objetivo do manejo: quando se objetiva priorizar o ganho de peso individual, recomenda-se a adoção da intensidade de 43,3%, que permite maiores consumos de lâminas foliares por cordeira; já quando se objetiva priorizar a produção por área, recomenda-se a utilização da intensidade de pastejo de 61,0%, pois sob essa intensidade o azevém permite maiores valores de taxa de lotação.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / It was studied growth dynamics of Andropogon lateralis, Aristida laevis, Axonopus affinis, Paspalum notatum and Paspalum plicatulum in a natural grassland of the Depressão Central of Rio Grande do Sul, subjected to rotational grazing with heifers, in the spring and summer of 2010/2011. The experimental design was completely randomized with 5 x 2 factorial arrangements, with two grazing intervals, defined by thermal sums of 375 and 750 degree-days, and five species of grasses. Leaf tissue flows, tiller dynamics and stability index of plant population of these grasses were assessed. The studied grasses had a higher leaf growth flow and balance between flows when subjected to grazing intervals of 750 degreedays and higher real efficiency of utilization, tiller appearance rate and site filling when subjected to grazing intervals of 375 degree-days. A. lateralis showed the highest flows of leaf growth, intake and senescence. A. laevis exhibited the lowest real efficiency of utilization. A. affinis and P. notatum had the highest rates of appearance of tillers. P. plicatulum was the only species with stability index of less than one in mean of the period and who had decline in their population of plants. Evaluation periods and/ or seasons influenced the number of senescent leaves per tiller, leaf intake flow, rates of appearance, survival and mortality of tillers and tiller population stability. Summer and, especially the month of December, represented a period of reproductive development of grasses. Intervals between grazing defined in order to respect the natural rhythms of growth of the species tend to optimize the productivity of natural grasslands. / Estudou-se a dinâmica de crescimento de Andropogon lateralis, Aristida laevis, Axonopus affinis, Paspalum notatum e Paspalum plicatulum em uma pastagem natural da Depressão Central do Rio Grande do Sul, submetida a pastoreio rotativo com novilhas, na primavera e verão de 2010/2011. O delineamento experimental foi inteiramente casualizado com arranjo fatorial 2 x 5, sendo dois intervalos entre pastoreios, definidos por somas térmicas de 375 e 750 graus-dia, e cinco espécies de gramíneas. Foram avaliados os fluxos de tecido foliar, a dinâmica de perfilhamento e índice de estabilidade da população de plantas dessas gramíneas. As gramíneas estudadas apresentaram maior fluxo de crescimento foliar e balanço entre os fluxos quando submetidas a intervalos de pastoreio de 750 graus-dia e maior eficiência real de utilização do pasto, taxa de aparecimento e ocupação de sítios quando submetidas a intervalos de pastoreio de 375 graus-dia. A. lateralis apresentou os maiores fluxos de crescimento, consumo e senescência foliar. A. laevis exibiu a menor eficiência real de utilização. A. affinis e P. notatum apresentam as maiores taxas de aparecimento de perfilhos. P. plicatulum foi a única espécie com índice de estabilidade inferior a um, na média do período experimenta e que apresentou declínio em sua população de plantas. Os períodos de avaliação e/ou a estações do ano influenciaram o número de folhas em senescência por perfilho, fluxo de consumo foliar, taxas de aparecimento, sobrevivência e mortalidade de perfilhos e índice de estabilidade da população de perfilhos. O verão e, principalmente o mês de dezembro, foi caracterizado como período de desenvolvimento reprodutivo das gramíneas. Intervalos entre pastoreios definidos de forma a respeitar os ritmos naturais de crescimento das espécies tendem a otimizar a produtividade das pastagens naturais.

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