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Fitohemijski skrining i procena antioksidantnog i antiinflamatornog potencijala sekundarnih biomolekula u vrstama roda Plantago L. / Phytochemical screening and evaluation of antioxidant and anti-inflammatory potential of secondary metabolites of Plantago L. speciesBeara (Krstić) Ivana 09 July 2010 (has links)
<p>Karakterizacija metanolnih ekstrakata jedanaest vrsta samoniklih bokvica (rod<em> Plantago L.</em>) obuhvatala je fitohemijski skrining i ispitivanje antioksidantne i antiinflamatorne aktivnosti. Primenom LC-MS/MS tehnike odreĊen je sadržaj odabranih sekundarnih biomolekula. Antioksidantna aktivnosti ekstrakata (sposobnost neutralizacije slobodnih radikala, redukcioni potencijal i inhibicija lipidne peroksidacije) ispitana je primenom spektrofotometrijskih metoda. U cilju odreĊivanja antiinflamatornog potencijala, razvijena je<em> in vitro </em>metoda za praćenje aktivnosti trombocitne ciklooksigenaze-1 i 12-lipoksigenaze. Svi ispitani ekstrakti pokazali su znaĉajnu biološku aktivnost.</p> / <p>Characterization of methanol extracts of eleven<em> Plantago L.</em> species included phytochemical screening and evaluation of antioxidant and anti-inflammatory activity. The content of several secondary metabolites was determined by LC-MS/MS technique. Antioxidant activity of extracts (radical scavenger capacity, reduction potential and inhibition of lipid peroxidation) was examined by spectrophotometric methods. With the intention to evaluate anti-inflammatory activity, an<em> in vitro method</em> was developed to measure activity of platelet cyclooxygenase-1 and 12-lipoxygenase. All examined extracts showed noticeable biological activity.</p>
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