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Genetic analysis of leaf rust resistance gene Lr34 in wheatDAKOURI, ABDULSALAM January 2010 (has links)
Effective at the adult plant stage, Lr34 is the most important resistance gene to leaf rust. Usage of closely linked molecular markers is the best strategy to facilitate the incorporation of economically important genes in an adapted plant germplasm. Ten novel molecular markers spanning the Lr34 locus were developed, including six microsatellites (cam), one insertion site-based polymorphism (caISBP), two single nucleotide polymorphisms (caSNP) and one indel marker (caIND). Marker caIND11 is the best diagnostic marker for marker assisted selection of Lr34. Two novel haplotypes of Lr34 were discovered in the germplasm. Analysis of these markers on five segregating populations revealed a recombination between caSNP4 and cam8 which provided further support for the identity of the ABC transporter as Lr34. Using Lr34-specific markers, the world collection (WC) was divided into five major haplotypes (H) of which H1 was consistently associated with the resistance phenotype Lr34+. SNP12-C is the functional unit of Lr34. Maximum parsimony network and other observations revealed that H4, an Lr34- haplotype, was probably the most ancient haplotype and H1 the most recent and that it likely arose after the advent of hexaploid wheat. Analysis of geographical distribution showed that H1 was at a high frequency in the Asian germplasm while H4 was more frequent in the European germplasm. Lr34, a gain of function mutation, was hypothesized to have originated in Asia. The (WC) was characterized for seedling and adult plant resistance using gene specific markers and gene postulation. Fourteen seedling genes were determined or postulated in the collection. Lr1, Lr10, Lr3 and Lr20 were the most highly represented genes while Lr9, Lr14b, Lr3ka and/or Lr30 and Lr26 were rare. The WC was evaluated for field resistance. The rust rating in the field ranged from nearly immune (1R) to highly susceptible (84S). Most Lr34 containing accessions had maximum rust severity (MRS) of 35%. The high levels of resistance in some accessions are likely the result of synergy between APR genes or between APR and seedling genes. Accessions that were highly resistant should be considered potential sources of resistance for future wheat breeding program to improve leaf rust resistance.
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Genetic analysis of leaf rust resistance gene Lr34 in wheatDAKOURI, ABDULSALAM January 2010 (has links)
Effective at the adult plant stage, Lr34 is the most important resistance gene to leaf rust. Usage of closely linked molecular markers is the best strategy to facilitate the incorporation of economically important genes in an adapted plant germplasm. Ten novel molecular markers spanning the Lr34 locus were developed, including six microsatellites (cam), one insertion site-based polymorphism (caISBP), two single nucleotide polymorphisms (caSNP) and one indel marker (caIND). Marker caIND11 is the best diagnostic marker for marker assisted selection of Lr34. Two novel haplotypes of Lr34 were discovered in the germplasm. Analysis of these markers on five segregating populations revealed a recombination between caSNP4 and cam8 which provided further support for the identity of the ABC transporter as Lr34. Using Lr34-specific markers, the world collection (WC) was divided into five major haplotypes (H) of which H1 was consistently associated with the resistance phenotype Lr34+. SNP12-C is the functional unit of Lr34. Maximum parsimony network and other observations revealed that H4, an Lr34- haplotype, was probably the most ancient haplotype and H1 the most recent and that it likely arose after the advent of hexaploid wheat. Analysis of geographical distribution showed that H1 was at a high frequency in the Asian germplasm while H4 was more frequent in the European germplasm. Lr34, a gain of function mutation, was hypothesized to have originated in Asia. The (WC) was characterized for seedling and adult plant resistance using gene specific markers and gene postulation. Fourteen seedling genes were determined or postulated in the collection. Lr1, Lr10, Lr3 and Lr20 were the most highly represented genes while Lr9, Lr14b, Lr3ka and/or Lr30 and Lr26 were rare. The WC was evaluated for field resistance. The rust rating in the field ranged from nearly immune (1R) to highly susceptible (84S). Most Lr34 containing accessions had maximum rust severity (MRS) of 35%. The high levels of resistance in some accessions are likely the result of synergy between APR genes or between APR and seedling genes. Accessions that were highly resistant should be considered potential sources of resistance for future wheat breeding program to improve leaf rust resistance.
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Genetic Screening Of Turkish Wheat Varieties For The Durable Resistance Gene, Lr34Boylu, Baris 01 April 2011 (has links) (PDF)
Wheat diseases such as rusts and powdery mildews are among the most
important and ancient diseases that affect wheat cultivation worldwide.
The pathogen race specific resistance genes cannot maintain long lasting
resistance. On the other hand, the presence of genes confers the non-race
specific resistance last much longer. The durable resistance phenotypes in
wheat against various rust and powdery mildew diseases were reported as
Lr34, Yr18, and Pm38 separately / nevertheless, they were known to locate
very close to each other based on linkage analysis. Recently, it was shown
that all of these resistance phenotypes are indeed conferred by the same
gene, encoding Adenosine triphosphate&ndash / binding cassette transporter
(ABC-transporter) type protein. The way with which this transporter is
functioning to maintain the durable resistance against different types of
pathogens is still unknown.
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The presence of LR34 (or ABC-transporter) gene resistant allele can be
detected by specially designed markers. In spite of the few DNA sequence
differences between the resistant and the susceptible alleles, the easily
applicable PCR based markers allow the detection of the presence of this
durable resistant allele on any given variety or cultivar. In our study, 62
different Turkish bread wheat varieties were screened by the gene specific
molecular markers, developed from those LR34 gene mutation sites. The
11 cultivars determined to contain the resistant allele. This study is the
first screening of Turkish cultivars for the presence of LR34 gene.
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