Two sections, Všeradice and Kosov, represent continuous sedimentary succession situated in the Prague Basin. No detailed research on stratigraphical level equal the N. kozlowskii graptolite zone focusing on chitinozoans, has not yet been conducted in the Prague Basin and is also very scarce globally. Limestone beds, examined for chitinozoans, were not very productive. Chitinozoan reaction on extinction is visible, if we compile data from both sections. The pre-extinct chitinozoan fauna is characterized by the Conochitinidae family. Samples from the post-extinct interval contained no chitinozoas. The interval of fauna recovery is characterized mainly by the Eisenackitina genera. In this interval, global zone for the uppermost Ludfordian, represented by E. barrandei, can be confirmed. In agreement with all the published literature, we have not found E. philipi, index taxa of the former global zone in the Prague Basin. E. lagenomorpha appears stratigraphically only slightly below E. barrandei and therefore it cannot substitute E. philipi, as it is used in Baltika region. chitinozoa, Prague Basin, Kozlowskii, Lau, extinction, Ludfordian, stratigraphy