Quality protein maize (QPM), high protein corn (HPC) and food grade maize (FGM) were processed into tortillas and direct expanded extruded snacks. QPM had similar test weight, density and kernel size with 45% more lysine and 38% more tryptophan compared to FGM. HPC had the largest kernel with density and test weight similar to FGM. During alkaline cooking, HPC absorbed water faster than QPM and FGM. White QPM required shorter cooking time and had less dry matter losses compared to FGM. All corn varieties had excellent pericarp removal at the optimum cooking time. Tortillas from QPM had better pliability and rollability after storage compared to FGM and HPC. HPC tortillas had lower rupture force after storage. The use of QPM for tortilla production may reduce energy and sewage cost, and could produce a tortilla with longer shelf stability with improved nutritional value. Decorticated and non-decorticated QPM, FGM and HPC grain were processed into corn meal and direct expanded snacks. A modified short scale dry milling system was used to produce the corn meal. QPM produced more coarse meal with greater fat content compared to FGM. Decortication decreased fiber content and coarse meal yield. Non-decorticated meal had greater protein, fiber and fat content compared to decorticated meal. The modified short flow milling system provides reduced lost fractions for extrusion into nutritionally improved products. Extrusion was performed in a low cost friction extruder. QPM extruded faster than FGM and HPC. FGM required greater specific mechanical energy than QPM. Extrudates from FGM were the most expanded followed by QPM and HPC. Extrudates from the three corn varieties were acceptable to the panelists and decortication did not affect acceptability. The improved nutritional value of QPM, was retained during dry milling and extrusion. Current QPM varieties can be processed into tortillas with longer shelf stability and meal for extrusion into a wide variety of snacks and other foods. These may have application in specialty health foods and in developing countries where maize is a staple food.

Local maize landraces have evolved over hundreds of years of natural and farmer selection under varying conditions. These landraces may have developed tolerance to abiotic stresses such as water deficits during this cycle of selection. However, despite its continued existence and importance, little is known on their agronomy and responses to water stress. If indeed landraces have developed tolerance to water stress, they may prove a key genetic resource for future crop improvement in light of increasing water scarcity. The primary objective of this study was to evaluate the responses of a local maize landrace to water stress at different stages of growth in comparison to two known commercial hybrids, SC701 and SR52. Seed from a local maize landrace was multiplied and characterised according to kernel colour. Two distinct colours were selected for the purposes of this study, white (Land A) and dark red (Land B). In a holistic approach, the thesis consisted of four separate studies whose overall objective was to evaluate the responses of the maize landraces to water stress at different growth stages, up to and including yield and its components. These comprised three controlled environment studies (25°C; 60% RH) and a field trial. For the controlled environment, two water regimes were used, 25% field capacity (FC) (stress treatment) and 75% FC (non-stress). The first study investigated the effect of water stress on early establishment performance. Seed quality was evaluated using the standard germination test together with electrolyte leakage. Catalase activity and accumulation of proline were examined as seedling physiological response to water stress. The second study was conducted as a pot trial to investigate the effect of water stress on growth, photosynthesis and yield. Photosynthesis was measured as chlorophyll fluorescence (CF). In addition, a field study over three planting dates was conducted at Ukulinga Research Farm in Pietermaritzburg, under dryland conditions, during the period from August 2008 to June 2009. The objective was to evaluate the effect of planting dates and changing soil water content on growth, yield and yield components. Three planting dates were used, representative of early (28 August 2008), optimum (21 October 2008) and late planting (9 January 2009). Lastly, a study on hydro-priming was conducted, necessitated by observations made primarily in the first study. The study was carried out under controlled environment conditions. The objective was to evaluate whether hydropriming can improve germination, vigour and emergence under water stress. Seeds were soaked in water for 0 hours (Un-primed or control), 12 hours (P12) and 24 hours (P24). Results from the first study showed that maize landraces were slower to germinate and emerge, and produced less vigorous seedlings compared to the hybrids. The study showed that hybrids were more superior under optimum (75% FC) conditions than under stress conditions (25% FC). Physiological showed that both hybrids and landraces expressed catalase under water stress, with landraces showing slightly better expression compared to the hybrids. Proline accumulation was observed in both hybrids and landraces as a response to water stress, with hybrids being more sensitive to water stress. In the pot trial, results showed that the vegetative stage of both hybrids and landraces was less sensitive to water stress than the reproductive stage. Results showed no differences between field capacities, with respect to emergence, mean emergence time, leaf number, CF, ear prolificacy and ear length. Photosynthesis, as measured by CF, was shown to be desiccation tolerant. Water stress had a negative effect on cob mass, lines per cob, grains per cob and total grain mass, and resulted in barrenness in the landraces. The hybrids had superior yield compared to the landraces. Results for the field trials showed that planting date had highly significant effects on emergence, plant height, leaf number and days to tasseling (DTT). Landraces emerged better than hybrids in all plantings; highest emergence was in the early and late plantings. Optimum and late planting resulted in maximum plant height and leaf number, respectively, compared to early planting. Hybrids were superior, growing taller and with more leaves than landraces in all plantings. DTT decreased with successive plantings. Planting date had an effect on ear prolificacy (EP), kernels/ear (KNE) and 100 grain mass. Planting date had no effect on ear length and mass, kernel rows/cob, grain mass and yield. With the exception of EP, hybrids out-yielded the landraces in all three planting dates. Hydro-priming landraces for 12 hours and 24 hours, respectively, improved germination velocity index, reduced mean germination time and improved emergence and mean emergence time of maize landraces under water stress. Performance of hybrid seeds remained superior to that of landraces even after seed treatment to improve germination and vigour. Landraces were slower to germinate and emerge and produced less vigorous seedlings in controlled conditions only. Both hybrids and landraces expressed catalase activity and also accumulated proline in response to water stress, although hybrids were more sensitive to stress in the establishment phase. Results confirmed literature, showing that, for both hybrids and landraces, the vegetative stage is less sensitive to stress than the reproductive stage. Hybrids produced superior yields compared to landraces in both controlled environment and field conditions. However, the pattern of seedling establishment observed in the initial controlled environment study for hybrids and landraces was reversed in the field study. Lastly, hydro-priming is of some benefit to maize establishment. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2009.

Maize is the most important grain crop produced in South Africa, serving as a food source for humans and animals, an input provider to other sectors, a source of job creation, a contributor of value added to the national economy, and an earner of foreign exchange. The South African maize industry plays an important role in the South African economy and consequently its role players should be supported to promote the industry. However, since the abolishment of the agricultural marketing boards and the deregulation of South African agriculture, farmers have suddenly found themselves exposed to global competition and a liberalised economy. Maize prices are uncertain and volatile, leading to increased risk. In addition, input prices have increased more rapidly than maize prices in some instances, and since no government protection exists, the cost squeeze effect places many farmers in a financial predicament. In order to mitigate the cost squeeze effect, farmers have started exploring farming methods and strategies that can improve their financial position. Precision farming (PF) is identified as a technological tool that can improve the profitability of a maize farm through higher yields and lower input costs, and can also indirectly assist in the general farm management and financial functions on the farm. The literature indicates that PF has been successfully implemented on various occasions with subsequent benefits, whether financial or qualitative. It could also be a useful tool to improve the profitability of South African maize farmers. Despite its various benefits, PF is associated with high capital expenditures and therefore farmers are reluctant to implement this technology on their farms. However, a PF service system that requires little capital expenditure is implemented by an agribusiness (Griekwaland-Wes Koöperasie) in the Northern Cape Province. Farmers who are part of this program only pay PF service fees that are charged on a perhectare basis. Most of the PF technologies and knowledge are provided by GWK and/or affiliated fertilizer companies, which subsequently mitigate the burden of high capital expenditures. The general objective of the study was to investigate the impact of PF on the profitability of selected maize irrigation farms in the Northern Cape Province. This was achieved by comparing the profitability and risk position of selected farms under a conventional farming (CF) system with the profitability of the same farms when converting to a PF system. The specific objectives of the study were to determine whether PF would generate better profits than CF; to determine whether PF would improve the farmer’s ability to repay his debt and generate an income (thereby improving the financial survivability of the farm); to determine whether PF would improve the debt-to-asset position of the farmer; and to determine whether PF is less risky than CF with respect to net farm income and cash position. The Bureau for Food and Agricultural Policy (BFAP) farm-level model developed by Strauss (2005) proved to be a useful tool to achieve the set objectives, since the BFAP farm-level model is linked with the BFAP sector model, which enables it to accurately analyse the impact of changes in policies and markets at both farm and sector level in South Africa. A positivistic approach was followed in order to answer the question, “What will the likely outcome be?” The model has the capacity to do simulations in both deterministic and stochastic modes. Three maize irrigation farms in the Northern Cape Province were chosen by a panel of agricultural specialists who are accustomed with the irrigation farms and PF system in this province. The farms were analysed by means of the BFAP farm-level model in order to determine the impact of PF on the profitability of each farm. The BFAP baseline of 2008 was used for this purpose. Key input variables were identified and simulated based on the BFAP baseline of 2008, as well as actual data, assumptions regarding PF and CF farming, and reported features and benefits associated with PF. In order to simulate the risk associated with CF and PF through stochastic modelling, correlated probability distributions were assigned to the relevant key input variables by de-trending the historical data of the key input variables. A correlation matrix based on the absolute deviation of a specific variable from its trend was subsequently constructed. Each variable was then simulated by means of a correlated empirical distribution, with 500 model iterations being run for each simulation in order to obtain stable probability distributions. From the results obtained in the study, the conclusion can be drawn that PF not only improves profit margins, but indirectly contributes to improved financial management. Considering the higher profit margins, more cash is at the disposal of the farmer. When this extra cash is again reinvested in the farming business, debt (in terms of production loans and medium- and long term loans) can be repaid more quickly and/or less debt has to be incurred, leading to lower interest payments that in turn further increase profit margins, ultimately improving the debt and cash position of the farm. The results also indicate that the risk position of the participating farms improved significantly with the implementation of PF. It can therefore be concluded that PF could also serve as a valuable risk management tool. From the discussions with the farmers it also became apparent that their overall farm management abilities were improved significantly, due to the informative nature of PF. Based on the results of this study, it can be concluded that the hypothesis as stated in Chapter 1 cannot be rejected. In addition, several other aspects pertaining to PF should be considered. Firstly, the results are applicable to the specific participating farms in the study only, and cannot be attributed to all maize farms in general. Secondly, despite a meticulous process of data verification and validation, the conclusions drawn in the study are based on the quality of the data provided by the stakeholders. Thirdly, factors such as farming operations, management decisions, market, weather and disease conditions might divert from the assumptions made in the study and thereby affect the actual results in future. Fourthly, since the study focuses solely on irrigation farming, a similar study can be conducted on dryland maize farming, since the majority of maize is produced under dryland conditions. Fifthly, the study could serve as a starting point for a comprehensive study on the impact of PF on maize farming throughout South Africa. Sixthly, the study could pave the way for an investigation into using PF as a tool to negotiate lower crop insurance premiums for farmers. Lastly, it would be useful to conduct a similar study on the impact of PF on maize farming where farmers are responsible for the acquisition of their own PF equipment, unlike on the participating farms where no extra capital expenditures were required. This could enable researchers to provide a better answer on the question of costs involved when converting to a PF system, as well as the ideal farm size in terms of economies of scale. Copyright / Dissertation (MScAgric)--University of Pretoria, 2010. / Agricultural Economics, Extension and Rural Development / unrestricted

Crop simulation models (CSMs) are used to evaluate management and environmental scenarios on crop growth and yields. Two corn (Zea Mays L.) crop growth simulation models, Hybrid-Maize, and CERES-Maize were calibrated and validated under Virginia conditions with the goal of better understanding corn response to variable environmental conditions and decreasing temporal yield variation. Calibration data were generated from small plot studies conducted at five site-years. Main plots were plant density (4.9, 6.2, 7.4, and 8.6 plants m-2); subplots were hybrids of differing relative maturity (RM) [early = Pioneer® Brand "34B97" (108 day RM); medium = Pioneer® Brand "33M54" (114 day RM); and late = Pioneer® Brand "31G66" (118 day RM)]. Model validation was generated from large scale, replicated strip plot trials conducted at various locations across Virginia in 2005 and 2006. Prior to model adjustments based on calibration data, both CSMs under predicted corn grain yield in calibration and validation studies. CERES-Maize grain yield prediction error was consistent across the range of tested plant density while accuracy of Hybrid-Maize varied with plant density. Hybrid-Maize-estimated biomass production was highly accurate. Greater leaf area index (LAI) and biomass production were measured than was predicted by the CERES-Maize CSM. Both CSMs were modified based on calibration data sets and validated. Validation results of the calibrated CSMs showed improved accuracy in simulating planting date and environmental effects on a range of corn hybrids grown throughout Virginia over two years. We expect that both modified models can be used for strategic research and management decisions in mid-Atlantic corn production. / Master of Science

Maize (Zea mays L.) production is constrained by a number of stresses, amongst the most important are gray leaf spot (GLS) caused by a fungus Cercospora zeaemaydis Tehon and E.Y. Daniels and Phaeosphaeria leaf spot (PLS) caused by Phaeosphaeria maydis (Henn.). The diverse germplasm comprising farmer collections and exotic material used in the medium and highland altitudes maize breeding programmes in western Kenya has not been improved for resistance to the two diseases. Heterotic patterns of germplasm from this region have also not been studied. Therefore, the objectives of this study were to (i) assess the prevalence, importance, and farmers’ perceptions of GLS and PLS, (ii) characterize maize germplasm collections into their heterotic groups and (iii) improve four maize populations for GLS and PLS resistance through recurrent selection. The participatory rural appraisal (PRA) was conducted at three sites in western Kenya during the 2005/2006 cropping season. Data was generated using a checklist in group discussions with 109 male and 123 female farmers as well as key informants. Constraints were identified and prioritised. The five most limiting, in order of importance, were low soil fertility, poor varieties and seed, drought, Striga, pests and diseases (GLS and PLS). Gray leaf spot and PLS were reported in all sites but farmers did not know the causes of these diseases. Farmers preferred local varieties Tiriki, Anzika and Kipindi due to their greater resistance to diseases than commercial hybrids. Farmer criteria for variety selection were low fertilizer, Striga and disease resistance, drought tolerance, closed tips, and high yield potential. Due to the high cost of hybrid seed farmers selected and planted their own seed from advanced generations from previous seasons. Across all the sites, yield gap between on-farm and expected yield potential was estimated as ranging from 4.73t ha-1 to 5.3t ha-1 mainly due to the identified constraints. Therefore maize breeding should focus on addressing important maize production constraints and farmers’ preferences identified in this study in developing varieties that will increase maize yields on-farm. During 2005/2006, seventy 77 testcrosses were developed through crossing 47 germplasm collections with four population testers, Kitale synthetic II (KSII), Ecuador 573 (EC 573), Pool A and Pool B. Crosses and testers were evaluated at Kakamega during 2006/2007 in a 9 x 9 triple lattice design. Significant (p < 0.05) differences in grain yield, ear height, days to 50% anthesis, GLS and PLS resistance were observed. Both general and specific combining ability effects (GCA and SCA, respectively) were significant (p < 0.01), with SCA accounting for more than 50% of the variation for GLS, PLS and yield and less than 50% for ear height, days to 50% anthesis and silk. This indicated that both additive and non-additive gene effects were important but non-additive gene effects were more important in conditioning these traits. High SCA effects indicated high heterosis between collections and populations. Both yield heterosis and SCA were used to study heterotic patterns, but percentage yield heterosis data was used to classify these materials into heterotic groups. Based on significance (p < 0.05) of percentage yield heterosis as a primary factor for classification, seven collections were classified to Pool A, 17 to Pool B, 12 to KSII and 6 to EC 573 heterotic groups. The study indicated that germplasm collections belong to distinct heterotic groups therefore they can be infused into these populations (Pool A, Pool B, KSII and EC 573). Four populations, KSII, EC 573, Pool A and Pool B were subjected to one cycle of reciprocal recurrent selection (RRS) and two cycles of simple recurrent selection (SRS) during the 2004-2006 cropping seasons at Kakamega. Response to selection was assessed by evaluating C0, C1 and C2 and four commercial checks in a randomised complete block design in three replications at Kakamega and Kitale during 2007. All cycles except C0 of Pool A were more resistant to GLS than the three checks, H623, KSTP94 and PHB3253. Response to selection for GLS was significant (p < 0.01) in the desired direction. Gains ranged from -32.2% to 6.4% cycle-1 for RRS and 0.0% to -61.3% cycle-1 for SRS. Heritability estimates of between 59% and 76.3% for GLS and 39% and 80% for PLS were observed indicating that both GLS and PLS can be improved through selection. Significant negative correlations between GLS and yield were observed in Pool A C0 (r = -0.947, p < 0.01) and between yield and PLS in Pool A C0 (r = -0.926, p < 0.01). These indicated gain in yield as GLS and PLS were selected against. Generally, SRS out performed RRS method both in genetic gain and time, as indicated by gain of -61% for SRS and -32.2% for RRS, respectively. Two cycles of selection were achieved in two years with SRS as compared to only one with RRS. These results clearly demonstrated that it is possible to improve for GLS resistance using simple and reciprocal recurrent selection methods. The main constraints to maize production in Western Kenya were low soil fertility, Striga, drought, lack of seed and diseases. Farmers preferred varieties that can do well under the constraints mentioned. Local collections belonged to distinct heterotic groups with good resistance to GLS and PLS and were highly heterotic to four maize population testers with both SCA and GCA effects being important in conditioning GLS and PLS resistance. Recurrent selection methods were found to improve maize resistance to GLS and PLS. Breeding should therefore, focus in development of hybrids and improvement of populations using these local collections by employing SRS and RRS selection methods with identified constraints and farmer preferences in mind. / Thesis (Ph.D.) - University of KwaZulu-Natal, Pietermaritzburg, 2007.

This thesis consists of two essays that each discuss a major component of the post-harvest management of maize in sub-Saharan Africa: drying and storage. The first essay uses cross-country data about on-farm storage decisions between 2013 and 2015 to assess the severity of storage loss in the absence of improved storage technologies. We find that while losses are low, farmers report on average that they lose more than expected and sell earlier than originally intended at harvest. Additionally, we look for evidence that farmers use adaptation strategies for the purpose of mitigating storage loss and find that storage chemicals are effective at both reducing loss and increasing storage duration. The second essay introduces a third-party moisture testing service to traders in western Kenya to elicit willingness to pay for external quality verification using two moisture detection devices, a low-cost hygrometer and a commercial grade moisture meter. We find that while traders value the moisture meter service more, the hygrometer service is more profitable for potential service providers. Further, when offered a chance to purchase the hygrometer device at/around market price ($2.50), only 15% of traders accepted the offer, suggesting that a service provider model is a viable way to make moisture testing more widely accessible and standard practice in the future.

Made available in DSpace on 2016-12-08T16:44:53Z (GMT). No. of bitstreams: 1 PGPV07MA014.pdf: 449670 bytes, checksum: 27f987f7db78d00331b32761b47a1875 (MD5) Previous issue date: 2007-02-16 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The increment in plant density is an alternative to maximize solar radiation interception and to increase maize grain yield. However, it can also reduce the crop photosynthetic activity and limit its efficiency to convert carbohydrates to grain production. Increases in maize hybrid s tolerance to crowding have been reported in different production areas around the world. Two traits that may contribute to this feature are the slower leaf senescence during grain filling and the higher uniformity in plant growth and development during the crop cycle. This work was carried out aiming to quantify the effects of plant population increment on the leaf area, grain yield and the variation coefficient of agronomic traits of maize cultivars with contrasting genetic variability. The trial was set in Lages, SC, Brazil. A randomized block experimental design disposed in split-plots was used. Three cultivars were evaluated in the main plot: an open-pollinated variety (Fortuna), a double-cross hybrid (Ag 303) and a single-cross hybrid (P30F53). Five plant populations were tested in the split-plot: 25,000, 50,000, 75,000, 100,000, and 125,000 plants ha-1. The experiment was sowed on 10-26-2005, with a row spacing of 0.70 m. When the crop had four expanded leaves, 10 plants of each split-plot that were in the same growth stage were labeled. These plants were used to measure leaf area and to follow the crop growth, determining the variation coefficient of leaf area, growth stage and plant height. These variables were estimated 10 times, at the growth stages of V4, V8, V12, V16, R1 (silking) and 14, 28, 42, 56 and 70 days after silking. After harvesting, the variation coefficient and grain production per plant were determined based on the 10 selected plants. In addition to that, grain production and yield components were determined. The data were submitted to the variance analysis by the F test, at the 5.0% significance level (P<0.05). The means were compared by the Tukey s test (P<0.05), by polynomial regression and linear correlation. The response of grain yield to increment in plant population was quadratic, regardless of cultivar. Grain yield of the singlecross hybrid (SCH) was higher and more responsive to increase in plant density than grain yield of the double-cross hybrid (DCH) and the open-pollinated variety (OPV). The plant densities that optimized grain yield were 86,665 plants ha-1, 53,044 plants ha-1 and 85,000 plants ha-1 for the SCH, DCH and OPV, respectively. The SCH presented higher leaf area values than the other cultivars 56 days after silking, when plant populations greater than 50,000 plants ha-1 were used. The highest variation coefficient value for grain production per plant was registered at 125,000 plants ha-1 and the lowest at 25,000 plants ha-1. The SCH presented smaller variation coefficient than the DCH and OPV for plant height and leaf area at silking, and for grain yield per plant. The higher morphologic uniformity of the SCH probably contributed to reduce intra-specific competition for water, light and nutrients, establishing a stronger sink after flowering. The SCH greater demand for photosynthetic products possibly favored the maintenance of leaf activity for a higer period, delaying senescence. Therefore, the slower leaf senescence and the more uniform plant growth contributed to the SCH higher grain yield and to the higher productivity differences between the SCH and the other two cultivars in the highest plant densities (75,000, 100,000 e 125,000 plants ha-1) / O incremento na densidade de plantas é uma forma de maximizar a interceptação da radiação solar e o rendimento de grãos do milho. Contudo, ele também pode reduzir a atividade fotossintética da planta e a eficiência de conversão dos fotoassimilados à produção de grãos. Aumentos na tolerância de híbridos de milho ao adensamento têm sido reportados em diferentes regiões produtoras no mundo. Duas características que podem contribuir para isto são a senescência foliar mais lenta no enchimento de grãos e a maior uniformidade no crescimento e desenvolvimento das plantas durante o ciclo da cultura. Este trabalho foi conduzido com os objetivos de quantificar o efeito do incremento da população de plantas sobre a área foliar, o rendimento de grãos e o coeficiente de variação de características agronômicas de cultivares de milho com bases genéticas contrastantes. O experimento foi conduzido no município de Lages-SC. O delineamento experimental utilizado foi o de blocos casualizados dispostos em parcelas subdivididas. Na parcela principal foram testadas três cultivares de milho: uma variedade de polinização aberta (Fortuna), um híbrido duplo (Ag303) e um híbrido simples (P30F53). Cinco populações de plantas foram avaliadas nas subparcelas: 25.000, 50.000, 75.000, 100.000 e 125.000 plantas ha-1. O experimento foi implantado no dia 26 de outubro de 2005, com espaçamento entre linhas de 0,70 m. Quando a cultura estava com quatro folhas expandidas, marcou-se 10 plantas de cada subparcela que estivessem no mesmo estádio fenológico. Estas plantas foram utilizadas para as avaliações de área foliar, bem como para o acompanhamento do crescimento, determinando-se o coeficiente de variação para área foliar, estádio fenológico e estatura de planta. Estas variáveis foram avaliadas 10 vezes, nos estádios V4, V8, V12, V16, R1 (espigamento) e aos 14, 28, 42, 56 e 70 dias após o espigamento. Após a colheita, foram determinados o coeficiente de variação e a produção de grãos por planta, nas 10 plantas selecionadas. Além disso, determinou-se a produção de grãos na área útil, bem como os componentes do rendimento. Os dados foram submetidos à análise de variância pelo teste F, ao nível de significância de 5% (P<0,05). As médias foram analisadas pelo teste de Tukey, a 5% de probabilidade de erro (P<0,05), por regressão polinomial e por correlação linear. A reposta do rendimento de grãos das três cultivares ao incremento na densidade de plantas foi quadrática. O rendimento de grãos do híbrido simples (HS) foi maior e mais responsivo ao incremento na população de plantas do que o híbrido duplo (HD) e da variedade de polinização aberta (VPA). As populações que otimizaram o rendimento de grãos foram de 86.665 plantas ha-1, 53.044 plantas ha-1 e 85.000 plantas ha-1 para o HS, HD e VPA, respectivamente. O HS apresentou maior valor de área foliar do que as demais cultivares aos 56 dias após o espigamento, nas densidades superiores a 50.000 plantas ha-1. Os maiores coeficientes de variação para produção de grãos por planta foram obtidos na densidade de 125.000 plantas ha-1 e os menores na de 25.000 plantas ha-1. O HS apresentou menor coeficiente de variação do que o HD e a VPA para estatura de planta e área foliar no espigamento, bem como para produção de grãos por planta. As maiores uniformidades morfológica e fenológica do HS provavelmente contribuiu para reduzir a competição intra-específica por água, luz e nutrientes, favorecendo o estabelecimento de um dreno mais forte logo após a floração. A maior demanda por fotoassimilados do HS possivelmente favoreceu a manutenção da atividade fotossintética das folhas por um período mais longo, retardando a senescência foliar. Portanto, a senescência foliar mais lenta e o desenvolvimento uniforme das plantas contribuíram para o maior rendimento de grãos do HS e para as maiores diferenças de produtividade registradas entre o HS e o HD e a VPA nas densidades mais altas (75.000, 100.000 e 125.000 plantas ha-1)

Master of Science / Department of Agricultural Economics / Timothy J. Dalton / In Kenya, agriculture governs the country’s fiscal economy, and this reliance on agriculture can cause both economic and hunger problems, a result of the country’s dependence upon rainfall for agricultural production. Kenyans must find ways to combat severe drought conditions; this can be accomplished through the adoption of inputs that decrease the probability of crop failure. The objective of this research is to determine whether variability exists in Kenyan maize yields, and whether or not specific inputs, specifically hybrid varieties, are either variance/skewness increasing or decreasing. The data used for this study was collected from a survey, designed by Egerton University’s Tegemeo Institute of Agricultural Policy and Development and Michigan State University, and administered in Kenya in the following years: 1997, 2000, 2004, and 2007. The survey identified factors of crop and field level production, such as inputs, crop mix, marketing data, and demographic information. This research makes use of only the 2007 data, comprising 1,397 households in total. The objectives of this thesis aim to go beyond the scope of typical production function regressions where yield is a function of a set of inputs, by examining further moments of yield, variance, and skewness to determine whether variability exists in Kenyan maize yields. Results indicate that variability does exist within Kenyan maize yields, often a result of differing input levels among households. In terms of overall impact of each variable on mean, variance, and skewness of maize yields, seed quantity, nitrogen use, and hybrid seed contribute the most to influencing these factors. In contrast, years of experience with hybrid maize, land tenure, terraced land and labor have the least influence on mean, variance and skewness within this research. Results also bring to light the popular debate against hybrid varieties versus open pollinated (OPV) or traditional varieties, and identify hybrid varieties as a source of variability in mean, variance and skewness of yields. Hybrid varieties should be paired with the knowledge of how to maximize yield in conjunction with other inputs, to give Kenya the opportunity to see substantial productivity gains throughout the country, especially in arid and semi-arid regions.

BACKGROUND:The opaque2 mutant is valuable for producing maize varieties with enhanced nutritional value. However, the exact mechanisms by which it improves protein quality and creates a soft endosperm texture are unclear. Given the importance of improving nutritional quality in grain crops, a better understanding of the physiological basis for these traits is necessary.RESULTS:In this study, we combined transcript profiling and proteomic analysis to better understand which genes and proteins are altered by opaque2 in the W64A inbred line. These analyses showed that the accumulation of some lysine-rich proteins, such as sorbitol dehydrogenase and glyceraldehyde3-phosphate dehydrogenase, was increased in mature kernels and may contribute substantially to the lysine content of opaque2 endosperm. Some defense proteins such as beta-glucosidase aggregating factor were strongly down regulated and may be regulated directly by opaque2. The mutant also had altered expression of a number of starch biosynthesis genes and this was associated with a more highly crystalline starch.CONCLUSIONS:The results of these studies revealed specific target genes that can be investigated to further improve nutritional quality and agronomic performance of high lysine maize lines, particularly those based on the presence of the opaque2 mutation. Alteration of amylopectin branching patterns in opaque2 starch could contribute to generation of the soft, starchy endosperm.

Since the 1990s, genetically modified (GM) agriculture has become a multi-billion dollar industry. Despite the rapid commercialization of GM crops in the United States, global controversy has slowed the adoption of the technology in developing countries. Yet, few studies have examined regulatory disputes outside of the United States and Europe. Debates in the United States and Europe focus on issues of human health and consumer choice. In other parts of the world, particularly Latin America, disputes center on the threats that GM agriculture poses to unique centers of biodiversity and food security, as well as issues related to bio-fuel expansion and the control over genetic resources and knowledge. My dissertation takes research on biotechnology in a new direction by analyzing the political process through which regulatory knowledge related to GM agriculture is negotiated, contested and reformulated. Guatemala is a key case to examine the politics of biotechnology regulation because despite strong US trade and transnational commercial interests, it is still illegal to grow biotech crops. The question becomes: what explains resistance to agricultural biotechnology? To address this issue, my dissertation focuses on three primary themes. First, I examine historical Mayan rural livelihood strategies within a context of political exclusion and state violence during the country's 36-year civil war. This history, in turn, informs a contemporary context characterized by the continued importance of subsistence-based corn production in the face of mounting rural inequality. Second, I contend that biotechnology regulatory debates in Guatemalan state institutions are integrally tied to a unique national context of corn biodiversity. I focus specifically on disputes between US-sponsored biotechnology regulations based on the principles of free trade and a more cautionary United Nations biosafety program. Third, I argue that resistance to agricultural biotechnology is bringing together diverse Guatemalan Mayan organizations until recently divided by the violence of the civil war. These organizations are deploying sophisticated cultural, economic and environmental knowledges that are effectively challenging efforts to commercialize GM agriculture. On a broader level, this study asserts that resistance to agricultural biotechnology is emblematic of broader struggles over the definition of legitimate knowledge in neoliberal development.