Early flowering is important for maize adaptation to short-season growing environments. Dwarfism, by preventing lodging, has the potential to increase grain yield. This thesis investigates three novel mutants of maize. The early flowering mutant (EarlyF) sheds pollen 1 to 5 days earlier than wild type plants. EarlyF, was shorter and developed fewer leaves than wild type plants, suggesting an earlier transition from vegetative to reproductive development. A candidate QTL for EarlyF maps to bin 7.03. The two allelic dwarf mutants, brachytic3-1 and brachytic3-2, have short internodes at maturity, resulting in severely reduced plant height. Despite being short, days to pollen shed and number of leaves were unchanged for both brachytic3-1 and brachytic3-2. brachytic3 maps to a ~ 7 Megabase region of bin 5.04. This thesis characterizes EarlyF, br3-1 and br3-2 and sets the stage for positionally cloning the mutations causing these mutants and has potential to contribute to maize improvement.

Temperate maize is one of few model species that relies mainly on endogenous indicators of the plant’s developmental stage to cue the onset of reproductive development. The INDETERMINATE1 (ID1) transcription factor is a key regulator of the floral transition and id1 mutants are very late-flowering. ID1 is expressed and remains localized in developing leaves, while florigenic signals originate in mature, photosynthetically active leaves. Since very little is known about the molecular components of the floral transition in maize, and in autonomously flowering species at large, this work utilized id1 mutants to analyze the transcriptional and physiological alterations associated with the floral transition in maize. Analyses of functional categories of transcriptional change between developing leaves of id1 non-flowering mutants and normal flowering maize suggest a role for ID1 in energy metabolism and epigenetic regulation of leaf development. In addition, a novel family of -glucosidase genes were found to be expressed exclusively in immature leaves of normal flowering maize in a pattern similar to the ID1 gene suggesting that these genes may act in concert downstream of ID1. Further, profiling of transcript and metabolite alterations in mature leaves, which are likely the source of floral cues, suggest that coordination of resource storage in the form of transitory starch is an important signal for floral promotion in maize. Finally, analysis of the floral transition in Balsas teosinte, the progenitor of modern maize and an obligate short-day plant, suggests that ID1 may define a regulatory module unique to the autonomous floral regulation pathway in maize and related grass species.

This thesis is an investigation of barrenness and plant-to-plant variability (PPV) in ear development of maize (Zea mays L.). A three-year field experiment was conducted on homogenous plants with similar initial plant size, development and uniform spatial patterns in parental inbred lines CG60, CG102 and their F1 hybrid CG60 × CG102. Physiological processes underlying barrenness were dissected into plant growth through development and dry matter partitioning to the ear at canopy, subpopulation, and primarily, individual plant levels. The growth and development of the ultimately barren individuals were followed from early vegetative stage to physiological maturity (PM) using a non-destructive allometric methodology. Plant-to-plant variability in ear development, related to plant development, was measured destructively from ear initiation to 1 wk after silking and at PM. Results showed that the individual plants exhibited differential responses to their previous growth and development in the two parental inbred lines. No physiological traits in growth and development or dry matter partitioning to the ear during the critical period bracketing silking could characterize individual barren plants. The F1 hybrid was resistant to barrenness even at 160,000 plants ha-1. At 80,000 plants ha-1, the spikelet number per row (SNPR) and spikelet number per ear (SNPE) exhibited less PPV around silking than earlier stages of development. For the three genotypes, PPV in plant morphological traits and ear length was relatively constant throughout development. In addition, the period around the kernel row number (KRN) formation stage was the only time-window that the PPV in stem volume, representing PPV in above-ground plant dry matter (PDM), affected PPV in SNPR and KRN for the three genotypes, with SNPR being more affected. Although the F1 produced greater PDM at silking and 1 wk after silking, it had shorter ear length and less ear dry matter than the two parental inbred lines at the corresponding stages. When the relationships are elucidated among early ear development, plant growth, leaftip development, and dry matter partitioning to the ear, during the vegetative to silking stages and under stress conditions, then the physiological processes underlying barrenness of the tested inbred lines could be further characterized. / Syngenta, the Ontario Research Fund, the Ontario Ministry of Agriculture, Food and Rural Affairs, and the Natural Sciences and Engineering Research Council of Canada

Research objective. The study was aimed to establish the competitive peculiarities of the multi-component agrocenose (maize, living mulches, weeds) and its effects on soil properties under sustainable farming conditions. Research tasks. To accomplish the research objective the following tasks were set - to study: 1. The influence of living mulches, grown in a maize monocrop, on soil physical properties (soil moisture dynamics, shear resistance, penetration resistance, soil structure and its stability), chemical characteristics (total nitrogen, available phosphorus and potassium content, pH) and biological characteristics (urease and saccharase activity); 2. The dynamics of biomass accumulation of living mulches and weeds; 3. The impact of living mulches on weed infestation in a maize crop and on weed seed contamination in a ploughlayer; 4. Maize crop irradiance conditions and their influence on different components of the agrophytocenose; 5. Projection coverage of maize inter-rows; 6. Maize productivity and biometric indicators; 7. The relationships between the factors tested. / Tyrimų tikslas – įvertinti daugiakomponentės agrocenozės – kukurūzų, tarpinių augalų, piktžolių – konkurencinius ypatumus ir jos poveikį dirvos savybėms tvaraus ūkininkavimo sąlygomis. Tyrimų uždaviniai. Tyrimų tikslui pasiekti numatyta ištirti: 1. Įsėlinių tarpinių augalų, augusių kukurūzų monopasėlyje, poveikį dirvos fizikinėms (dirvos drėgnio dinamikai, šlyties pasipriešinimui, kietumui, dirvos struktūrai ir jos patvarumui), cheminėms (suminio azoto, judriojo fosforo ir kalio kiekiui, pH), biologinėms (ureazės ir sacharazės aktyvumui) savybėms; 2. Įsėlinių tarpinių augalų ir piktžolių biomasės kaupimosi dinamiką; 3. Įsėlinių tarpinių augalų poveikį kukurūzų pasėlio piktžolėtumui bei armens užterštumui piktžolių sėklomis; 4. Kukurūzų pasėlio apšvitos sąlygas ir jų poveikį skirtingiems agrofitocenozės komponentams; 5. Kukurūzų tarpueilių projekcinį padengimą; 6. Kukurūzų produktyvumo ir biometrinius rodiklius; 7. Tiriamųjų veiksnių sąsajas ir poveikio priežastingumą.

A sample of 260 farm households that were randomly selected in Katumba ward, Rungwe district, Tanzania were studied for the effects of storage methods on the quality of maize grain and household food security using qualitative and quantitative methods. Maize storage problems, amounts of maize that farm households harvested and amounts of maize that farm households lost to pests per year, food security status and farm households’ perceptions concerning their food security status were investigated using face - to - face semi - structured and structured interviews. Common storage methods that farm households used to store maize and the dietary importance of maize were investigated through interviews, seasonal calendars and the matrix for scoring and ranking. The quality of maize was investigated through conducting mycological analysis and through investigating levels of insect infestation using the incubation method on maize samples collected from a sub-sample of 130 farm households at harvest and after five months of storage period. It was found that farm households in Katumba ward preferred maize meal rather than other types of food that provide bulk such as rice and green bananas/plantains. Maize contributed 66.8 % - 69.5 % of the total energy and 83 - 90 % of the total protein required per day, and farm households stored maize using roof and sack storage methods. It was also found that 34.5 % of 2323 tonnes of maize that were harvested per annum in Katumba ward were lost to pests during storage. Fusarium, Diplodia, Aspergillus and Penicilliums species were identified as the main fungal pathogens that attacked stored maize. Sitophilus zeamais, Sitotroga cerealella and rodents were also identified as the main maize storage pests. About 25 % of the maize samples that were collected at harvest and 93 % of the maize samples that were collected from the same farm households after five months of storage were infested by either Sitophilus zeamais or Sitotroga cerealella or both. Maize samples from the two storage systems had an average number of 80 insect pests per 120 maize kernels (or 51 g of maize), amounting to 1569 insects per kg. The high levels of insect infestation reduced the amount of maize that could have been available to the farm households and subjected stored maize to fungal infections and subsequent contaminations, thus, rendering the farm households vulnerable to food insecurity. Furthermore, it was also found that most of the infestation of maize by insect pests and moulds in Katumba ward occurred during storage, and that farm households were not well informed concerning maize storage and the negative effects that fungal activities in maize can have on the health of the consumers. An average of 87717 μg/kg fumonisins, 596 μg/kg aflatoxins, 745 μg/kg ochratoxins and 1803 μg/kg T-2 toxins were detected in the maize samples. Currently, there are no set standards for T-2 toxins, whereas the internationally accepted standards for aflatoxins, fumonisins and ochratoxins in cereals are 20 μg/kg, 4 mg/kg and 50 μg/kg, respectively. It was concluded that the levels of mycotoxins detected in maize from Katumba ward were far above the internationally accepted standards and that the farm households were at risk of ill health through consuming maize meals made from contaminated maize grain. The presence of high concentrations of mycotoxins, together with the high levels of insect infestation in the maize led to the conclusion that reduction of the nutrient content of the maize grain in Katumba ward was inevitable. Thus, the pests that infested maize stored using the roof and sack storage methods in this ward compromised not only the availability of food, but also the utilization of the nutrients in the maize and its safety, leading to the farm households’ food insecurity. It was further concluded that the quality of maize stored using roof and sack storage methods in Katumba ward was low and that the roof and sack storage methods were inadequate for protecting stored maize from pests. It was recommended that an efficient method for rapid drying of maize prior to storage be found, that the roof and sack storage methods be improved so that they can effectively protect stored maize from moisture content problems. It was also recommended that the farm households’ awareness concerning maize storage and food security be raised, and that the extension staff in Katumba ward should urge the Tanzanian government to implement an agricultural policy which promotes efficient maize storage and maize quality in order to improve the current status quo. Above all, since maize is the predominant staple, it was recommended that the maize breeding program in Tanzania should emphasize development of maize varieties that are resistant to ear rots, storage insects and to contamination by mycotoxins as part of a larger program to improve food security in this part of the country. Breeding programs that aim at enhancing the nutritional value of maize were also recommended. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.

Quality protein maize (QPM) has high nutritional value, but production is threatened by downy mildew (DM) and maize streak virus disease (MSVD) among other constraints. There are few studies of DM and MSVD resistance in QPM cultivars. The objective of this study was to improve resistance to DM and MSVD in three QPM populations. This was realized through ascertaining farmers’ key production constraints and special preferences for cultivars; determining the utility of recurrent selection method for improvement of three QPM populations (SussumaS2, ZM521Q and Pop62SRQ); and determining grain yield potential. The study was conducted in Mozambique for DM and in Zimbabwe for MSV, during 2003 to 2006. Surveys were conducted in Manica and Angonia districts in Mozambique to ascertain farmers’ perceptions and preferences for maize varieties, especially QPM. Participatory rural appraisal tools that included semi-structured questionnaires and focus group discussions were used to collect data. Results showed that farmers predominantly grew open pollinated varieties and fewer normal maize hybrids (non-QPM), and grain yield was estimated to be very low (0.2 to 0.6 t ha-1). Results showed that drought and insect pests were the dominant constraints to maize productivity in Mozambique, while diseases were ranked third. Downy mildew disease and MSVD were considered to be the most important diseases reducing maize productivity. Farmers also showed high preference for high yielding and early maturity cultivars in all areas. Predominantly, farmers were still using their local landraces because of sweet taste, particularly for home consumption and flint grain for storage. Farmers’ access to improved cultivars was limited due to high seed prices on the local market. Research priorities as perceived by the farmers included breeding for resistance to drought, grain weevils and diseases and sweetness. Generally, farmers showed little knowledge of QPM varieties and the importance of this trait, but they observed that the few QPM varieties they knew had some weaknesses such as poor storability and susceptibility to DM and MSVD which required improvement. These results should be considered in breeding new cultivars, both normal and QPM. To improve DM and MSV disease resistance in QPM varieties, S1 recurrent selection was conducted in three QPM populations, Sussuma, ZM521Q and Pop62SRQ at Umbeluzi Research Station in Mozambique and at CIMMYT-Harare Research Quality protein maize (QPM) has high nutritional value, but production is threatened by downy mildew (DM) and maize streak virus disease (MSVD) among other constraints. There are few studies of DM and MSVD resistance in QPM cultivars. The objective of this study was to improve resistance to DM and MSVD in three QPM populations. This was realized through ascertaining farmers’ key production constraints and special preferences for cultivars; determining the utility of recurrent selection method for improvement of three QPM populations (SussumaS2, ZM521Q and Pop62SRQ); and determining grain yield potential. The study was conducted in Mozambique for DM and in Zimbabwe for MSV, during 2003 to 2006. Surveys were conducted in Manica and Angonia districts in Mozambique to ascertain farmers’ perceptions and preferences for maize varieties, especially QPM. Participatory rural appraisal tools that included semi-structured questionnaires and focus group discussions were used to collect data. Results showed that farmers predominantly grew open pollinated varieties and fewer normal maize hybrids (non-QPM), and grain yield was estimated to be very low (0.2 to 0.6 t ha-1). Results showed that drought and insect pests were the dominant constraints to maize productivity in Mozambique, while diseases were ranked third. Downy mildew disease and MSVD were considered to be the most important diseases reducing maize productivity. Farmers also showed high preference for high yielding and early maturity cultivars in all areas. Predominantly, farmers were still using their local landraces because of sweet taste, particularly for home consumption and flint grain for storage. Farmers’ access to improved cultivars was limited due to high seed prices on the local market. Research priorities as perceived by the farmers included breeding for resistance to drought, grain weevils and diseases and sweetness. Generally, farmers showed little knowledge of QPM varieties and the importance of this trait, but they observed that the few QPM varieties they knew had some weaknesses such as poor storability and susceptibility to DM and MSVD which required improvement. These results should be considered in breeding new cultivars, both normal and QPM. To improve DM and MSV disease resistance in QPM varieties, S1 recurrent selection was conducted in three QPM populations, Sussuma, ZM521Q and Pop62SRQ at Umbeluzi Research Station in Mozambique and at CIMMYT-Harare Research. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2007.

The consequences of toxigenic fungi associated with stored seed have stimulated these investigations aimed at developing treatments to minimise this mycoflora, without significantly reducing seed quality or viability. The effects of immersion in water at 55, 57 and 60 QC for durations of 5 to 60 min were assessed for maize (Zea mays L.) seed in terms of fungal status, water uptake, electrolyte leakage, germination and seedling establishment. These assessments were conducted immediately after treatment, after re-dehydration for 2 days in an ambient air stream, and following a 1 month storage period under either cold (4 QC) or ambient (25 QC) conditions (33% and 91% RH, respectively). In all cases, the results are compared with those of control seeds and seeds pre-imbibed for 4 h at ambient temperature. The level of internal contamination, represented almost entirely by Fusarium moniliforme Sheldon, declined significantly when assessed immediately after treatment, the efficacy of which increased with increasing temperature and duration of treatment. Seeds immersed in water at 55 QC for a duration of 15 min exhibited an 85% reduction in infection levels, when compared with those of the control, while those treated at 57 and 60 QC (same duration) were uninfected. Immersing seeds in hot water, however, resulted in a lag in germination rate and drop in germination totality, the degree of which was enhanced by increasing duration and temperature of treatment, suggesting the status of the manipulation to be an accelerated ageing treatment. The electrolyte leakage studies indicated that the reduced germination performance of these seeds was not due to plasmalemma disorganisation. These deleterious effects, however, were counter-balanced as seeds treated at 55, 57 and 60 QC for durations up to 60, 30 and 10 min, respectively, produced plants of superior quality than those of the control, which is ascribed to the reduction of systemically transmitted pathogens. The efficacy of the hot water treatment in reducing the levels of seed infection and improving seedling quality was enhanced by subsequent re-dehydration. The reduction in seed-associated mycoflora was maintained following storage for 1 month at both 4 QC (33% RH) and 25 QC (91% RH). However, both seed and seedling quality were adversely affected following storage even under cold, dry conditions, which may be a consequence of the pre-treatment history of the seeds, which had been cold-stored for two years prior to the experiments. Applied as a pre-sowing treatment, therefore, hot water treatment shows promise for producing a crop of superior quality, less prone to fusarial pathogenesis. This treatment may be of particular importance to Third-World subsistence communities. / Thesis (M.Sc.)-University of Natal, 1995.

The effects of urbanisation on maize consumption are considered in the context of Gauteng as it is almost completely urbanized (96%) and would have data that would be more readily available and up to date. Anecdotal evidence of a per capita decline in maize consumption is based on an expected greater degree of employment of the population. A greater proportion of women in the workforce is also expected placing time constraints on households. The resulting expected increase of disposable income combined with time constraints would cause higher consumption rates of convenience foods. The envisaged declining trend of per capita maize consumption necessitated a need to discuss Engel's Law which states with rising incomes, the proportional share of expenditure on food declines (Engel, 1877). Bennett's Law is also discussed which is an empirical generalization of an inverse relationship between the percentage of total calories derived from cereals and other staple foods and per capita income (Bennett, 1954). The population demographics of Gauteng are analysed, the main reference being a detailed comparison between the 1996 and 2001 censuses. Where data is available trends are extrapolated using South African Statistical Services' mid-year population estimates for 2004 (STATS SA, 2004). The demographics of the black African population, as the dominant consumers of maize meal, are paid particular attention. It was revealed that unemployment and poverty rates are increasing placing considerable pressure on, in particular, black African households. It was also found that black African income levels had not increased to the degree of other population groups. The HIV/AIDS pandemic, affecting mainly the black African population and mostly of an employable age, is considered. Mortality rates attributed to AIDS are controversial but an irrefutable dramatic declining life expectancy of the national population was found placing additional strain on household budgets. Also considered are eating habits by population group and by region made available by the Bureau of Market Research. / Thesis (MBA-University of KwaZulu-Natal, 2006.

Agricultural intensification to increase food, feed, and fibre production has also resulted in environmental degradation, including poorer soil quality and high emissions of greenhouse gases (GHGs) like nitrous oxide (N2O). Intercropping, an agroecosystem management practice where more than one crop is planted on the same plot of land at the same time, promotes the complementary use of soil nutrients, and may improve soil quality and increase the retention of inorganic nitrogen (N) in the soil, thereby reducing N2O emissions. An experiment was conducted in Balcarce, Argentina to determine the impact of intercropping maize (Zea mays L.) and soybean (Glycine max (L.) Merr.), (either 1:2 or 2:3 rows of maize to soybean) on soil quality and soil N transformations after six cropping seasons. It was found that intercropping significantly improved soil quality over a six year period, as indicated by the soil organic carbon (SOC), soil total nitrogen (TN), soil light fraction organic matter (LF), and soil microbial biomass carbon (SMB-C). However, the soil quality also significantly improved in the sole crops over this time, and in 2012, only SMB-C was significantly (p<0.05) greater in the 2:3 intercrop than in the sole crops. Intercropping resulted in higher rates of gross nitrogen (N) mineralization than the sole crops, and the 2:3 intercrop resulted in higher rates of gross N immobilization than in the other treatments. However, the high rate of gross N mineralization resulted in a low relative NH4+ immobilization in both intercrops, signifying a lower potential for reducing soil NH4+ concentrations than in the sole crop treatments. Net N immobilization occurred in all treatment plots, which was desired at the end of the fallow period to reduce N losses from the soil. The 2:3 intercrop appeared to perform better than the 1:2 intercrop. However, further research needs to be conducted to determine the seasonal variations in N mineralization and immobilization, and to further examine the intercrop spatial arrangements to increase crop residue yield.

Produsente van landboukommoditeite, veral in ontwikkelende lande, word blootgestel aan prysrisiko's. Markte vir landboukommoditeite in Suid-Afrika, soos in die res van die wêreld, is die afgelope aantal jare gekenmerk deur prosesse van deregulering. Die bemarkingsrade wat aanvanklik verantwoordelik was vir die bemarking van landbouprodukte, het ontbind en produsente van landbouprodukte moes nuwe metodes vind om hulle produkte te bemark. Een van die metodes wat gebruik word, is termynkontrakte. Witmielies en geelmielies is die twee landboukommoditeite wat in die grootste hoeveelhede geproduseer word in Suid-Afrika. Witmielies en geelmielies word as twee afsonderlike kommoditeite verhandel op die termynbeurs. Witmielies word hoofsaaklik aangewend vir menslike verbruik en geelmielies vir dierevoer. Hierdie studie fokus hoofsaaklik op witmielies. Die prys van mielies word beïnvloed deur veranderinge in die vraag daarna en aanbod daarvan. Faktore wat die vraag en aanbod van mielies beïnvloed is, onder andere, oesskattings, reënval, die wisselkoers en die pryse van mielies op die buitelandse mark, veral die markte in die VSA. In Suid-Afrika vorm die invoerpariteit en uitvoerpariteit 'n band waarbinne die prys van mielies varieer. Die doel van hierdie studie is om 'n regressievergelyking te konstrueer ten einde prys van die witmielietermynkontrakte te verklaar. Die regressie-analise word deur middel van 'n foutherstellende model met outoregressiewe foutterme behartig. Die regressie-analise slaag daarin om die prys van witmielietermynkontrakte te verklaar. / Thesis (M.Com. (Economics))--North-West University, Potchefstroom Campus, 2004.