The dissertation be about use the opportunity of 3D modelling geological settings for definitions engineering geology zoning. The model area represents the map sheet 6 - 8 Kralupy nad Vltavou in scale 1:5 000, which is built by rocks (pre-Quaternary) and soils (Quaternary cover) divided into eleven engineering geology zones. The results of modelling are showing at the map appendices, which are engineering maps all of nine the individual roof (surface) of zones, including the 3D section in the direction of x axis and also five of the 2D maps of roof of selected zones with the 3D schematic models. The integral part is the map of documentation points and their database on DVD. Three dimensional modelling presents a benefit mainly therefore the solutions, where are visible the spatial geological settings including the thickness individual layers, respectively zones. These display options can have practically used at designing underground buildings, e.g. tunnels, where would it mean shortening time the preparation of the project. Modelling of the zones also shown a possibility of distinction the mistakes at some archive hole. For example, the mistakes in descriptions can relate to confusion of Neogene's sand layer for Quaternary's sand. In the description of new geological survey, when using appendix no....

The aim of this study was to carry out an analysis of a protein composition of diferent crop species using the method of peptide mass mapping. The samples were analysed using analytical method LC/ESI-Q-TOF and the acquired spectra were compared with the plant protein databases. The origin of the found plants was discussed in the aspect of the reliability of the used method considering the taxonomical relationships in the taxonomical group. The results of the analysis were compared with the food composition specified by the producer, and it was evaluated, if it is possible to verify the protein composition by using the applied method. In case of maize (Zea mays) this method is considered as reliable, because all of the found proteins were indentified as maize proteins. In case of rice (Oryza sativa) and pea (Pisum sativum), only one, respectively, two proteins of different plants species were found in the number of the relevant plant proteins. This method was evaluated as not so reliable for samples made of cereals (Poaceae). It is possible to conclude, whether the product comes from Poaceae or not, but it is impossible to verify the taxon of genus or the inferior taxons. This fact was in a great extent disscused in this thesis - the reason is probably the resemblance of Poaceae, and the...

This thesis deals with snow cover mapping by using time-series of SAR images of the sensors ENVISAT ASAR and TerraSAR-X. The methodology is based on the so-called Nagler's algorithm, which is based on determination of the change of absorption of radar signal due to the liquid water content in the snow cover. The resulting ratio image is classified into the areas with wet snow or without it according to the selected threshold value. The results are compared with the maps of snow cover derived from MODIS optical data and with data from meteorological stations of CHMI. The main aims of this work are to suggest most suitable conditions (time of the year, weather) for acquisition of reference images, to find the change of the threshold value with respect the chosen reference image and the type of land cover. The same methodology should then be applied on the radar data of shorter wavelength. The obtained results will be further used for improving the methodology of snow cover mapping from SAR data in the Czech Republic.

This work introduces a database of experimentally verified structures of nucleic acids which were collected from published scientific literature. The database is annotated and the structures are analysed from the perspective of quality and it was found that the experimentally obtained data are not always sufficient - their supporting evidence is often limited and their quality is not convincing. This work also discusses some of the problems, that can be encountered when the structures are experimentally probed. Contents of the database were compared to the RFAM database and despite of its small range it contains 80 new structures. The complete database of 166 structures can be possibly used to optimise software used to predict derived structures of nucleic acids. Furthermore, the work presents several possible ways of improvement of the quality of contained structures.

This thesis is focused on the example of process approach used as the tool for quality management in a real company. The aim of this paper is to get readers acquaint with quality control management eight principles from the ISO/IEC 9001 perspective. The thesis then elaborates the practical site of process management approach. The paper describes process management evolution stages, its benefits, there are introduced specific types of modelling methods and ways for process performance measurement. The implementation of process approach is then shown on practical example. The thesis's practical part provides process charts for key organization processes drafted according to the one selected modelling method, descriptions for such processes, suggestions for change aiming to process optimisation and finally also proposes measurable process performance indicators.

Understanding protein function highly benefits from the knowledge of its three-dimensional structure, especially in the case of protein-ligand complexes. Structural biology methods such as X-ray crystallography, SAXS and NMR are therefore widely used for structural studies of protein-ligand interaction. In this work, these methods were used to understand two biological processes involving protein interactions: X-ray structural analysis was used to study binding of effector molecule to a prokaryotic transcription factor. NMR and SAXS techniques were used to study interaction of a monoclonal antibody with its protein antigen. Transcriptional regulator DeoR negatively regulates the expression of catabolic genes for the utilization of deoxyribonucleosides and deoxyribose in Bacillus subtilis. DeoR comprises an N-terminal DNA-binding domain and a C-terminal effector-binding domain (C-DeoR), and its function is regulated by binding of a small-molecular effector deoxyribose-5-phosphate. We determined crystal structures of C-DeoR both in the free form and in complex with deoxyribose-5-phosphate. Structural analysis revealed unique covalent binding of effector molecule through a reversible Schiff-base double bond with an effector-binding-site lysine residue. The physiological nature of this binding mode was...

Southwest base of Cordillera Blanca mts. in South American Ands belongs to the most tectonically active fault zones. The core of this thesis was making of a detailed geomorphological map based on remote sensing satellite images for the purpose of analysis of direct and indirect manifestations of neotectonics. The greatest emphasis was placed on morphological manifestations of tectonics movements which are fault scarps facets and vertical fault steps deforming other shapes of relief - e.g. lateral moraines, dejection cones. Vertical steps reach from a few meters to tens of meters (max. 60 m) according to the age of influenced landform. Evaluation of measurements from deformometr from locality Pitec shows main trends of movements - moderate rise of massif, slow opening of fault plane and negligible left-lateral slip. Calculation of mountain-front sinuosity index, whose values were found relatively low, confirms high tectonic activity of fault zone. Analysis of longitudinal stream profiles hasn't showed presence of knick-points in the place of crossing with fault zone. Non-continuous process of lifting movements, whose interval is estimated at 1ka to 3ka (while the very last 2ka has been in relative tectonic calm), could be a possible explanation. Deep erosion of water streams wiped the tectonics...

This thesis deals with mapping of the small folk architecture, such as crosses, small chapels and devotional pillars, in area of microregion of Stražiště. The introductory part of this work contains theoretical introduction to folk architecture. Next part deals with typology of the small folk architecture, its origins, functions and reasons for destruction. Third part of this work deals with field research, during that were taken photographs of each object and written necessary attributes. Based on this information was created the catalog, which can be found in enclosed CD. Last part of this work deals with selected objects in each cadastral area and statistics connected with them.

My dissertation thesis The interface between city and the landscape, centers on an area of the locally prominent rock Marianska skala in the city Usti nad Labem. The final design is a form of urbanistic-architectural study. The design methodology is based on city analysis and public research as mental mapping. The subject area has strong potential and natural values, however at present there is a minimal usage of this area by the local residents. The project therefore aims to integrate the area into a functional city system, with the rock becoming a key post of the connection between city center and the surrounding landscape. By addressing the challenges of integrating the city into the fabric of the surrounding landscape, the project explores the limits and possibilities of permeability. The thesis introduces aspects of the historical interface between city and the landscape, and this is the main principle the thesis is based on. New functions and elements as a forest park, as well as a sport and recreation area, are designed to revive the site and surrounding area. Designed for residents and tourists alike, creating a new dialogue between users, the historical landscape, and modern concepts of sport, tourism and ecology. Emphasis is put on the natural values and their protection. The design of the individual parts combine to promote and celebrate the rock Marianska skala as an important orientation point and vertically dominant feature of the city structure.

Protein misfolding is considered to be the major pathogenic mechanism in homocystinuria due to cystathionine beta-synthase (CBS) deficiency. The aim of this work was to study molecular mechanisms underlying protein misfolding of CBS mutants. Firstly, we studied spatial arrangement of normal human CBS protein. Using data from differential covalent labeling of solvent-exposed aminoacid residues, we identified interdomain contact area between the catalytic core and the regulatory domain in human CBS, and we subsequently generated the structural model of the full-length CBS. In the next step, we studied evolutionary divergence of CBS protein structures. We performed phylogenetic analysis that revealed unique spatial arrangement of CBS enzyme in nematodes; the domain architecture of CBS in Caenorhabditis elegans was studied experimentally in more detail. Finally, we determined conformational properties of a representative set of human CBS mutants that exhibited in various extent affected formation of tetramers and decreased catalytic activity. Using thermolysin-based proteolytic techniques for analysis of nine mutants expressed in E.coli, we found that an unfolded structure is a common intermediate occurring in CBS misfolding. The importance of protein unfolding for pathogenesis of CBS deficiency was...