La morphogenèse aérienne inclut des caractères de croissance, de développement et de phénologie, et conditionne fortement la valeur d'usage des Légumineuses. Parmi ces caractères, la floraison est un événement majeur du cycle de vie car elle est déterminante pour le succès reproductif. Elle correspond à la transition généralement non réversible d'un méristème végétatif produisant des feuilles et tiges, en un méristème floral. La régulation de ce phénomène morphogénétique est le fait d'un réseau complexe de signalisations. Les légumineuses cultivées ont souvent des génomes complexes. C'est le cas de la luzerne (Medicago sativa), espèce fourragère pérenne, tétraploïde et allogame ainsi que du pois (P. sativum) qui présente un génome de grande taille. Des études précises peuvent être menées sur la légumineuse modèle Medicago truncatula, espèce diploïde, annuelle, à cycle court et autogame. De nombreuses ressources génétiques et génomiques sont disponibles chez cette espèce qui possède un fort degré de synténie avec la luzerne et le pois. De plus des gènes intervenant dans le déterminisme de la date de floraison ont été décrits chez A. thaliana et chez le pois. L'objectif de la thèse est d'identifier des zones du génome et des gènes en utilisant les connaissances et outils développés chez M. truncatula, A. thaliana et P. sativum dans le déterminisme génétique de la date de floraison chez M. truncatula. Après une analyse de l'effet de la photopériode sur la date de floraison d'une gamme de lignées, une approche " gènes candidats positionnels " a été mise en oeuvre. La méthodologie employée consiste à montrer la variabilité génétique de la date de floraison en réponse à la photopériode, rechercher des QTL (Quantitative Trait Locus) de date de floraison dans trois populations connectées de lignées recombinantes, réaliser une méta-analyse QTL afin de détecter les régions conservées dans le contrôle du caractère entre populations, cartographier finement un QTL majeur et repérer les gènes candidats présents dans son intervalle de confiance. L'expression de ces gènes a été comparée pour deux lignées afin d'associer au caractère les gènes différentiellement exprimés. En chambre de culture, la date de floraison de huit lignées a été mesurée sous deux traitements : 12 heures et 18 heures d'éclairement. Les données montrent qu'il existe de la variabilité génétique pour la date de floraison entre ces huit lignées, que la floraison est plus précoce en jours longs qu'en jours courts et qu'il existe une interaction lignée x photopériode. Un QTL majeur de date de floraison a pu être repéré sur le groupe de liaison 7 dans les trois populations de lignées recombinantes expliquant de 10 à 60 % de la variabilité observée. En méta-analyse sur les trois populations, un QTL consensus a été mis en évidence ayant un intervalle de confiance de seulement 0.9 cM. La cartographie fine de ce QTL a été réalisée sur la descendance (1663 plantes) d'une plante F6 hétérozygote au QTL détecté dans la population LR4. L'intervalle du QTL ainsi détecté couvre 2.4 cM. Six gènes homologues de gènes de floraison décrits chez A. thaliana ont été identifiés dans l'intervalle de ce QTL établi par cartographie fine. Leur séquençage pleine longueur a révélé du polymorphisme entre les deux parents : pour MtCO, homologue de CONSTANS et pour MtFTLc, homologue de FT. Par contre, aucun polymorphisme n'a été détecté pour deux autres homologues de FT (MtFTLa et MtFTLb) ni pour PKS. Une analyse de l'expression différentielle par RT-PCR semi quantitative des six gènes candidats a été réalisée chez deux lignées parentales contrastées pour leur date de floraison. Seul le gène MtCO est différentiellement exprimé entre ces deux lignées ; ce gène est donc actuellement le principal candidat pour expliquer la variation du caractère révélée à ce QTL sur le chromosome7, dans ces populations.

Symbiotic nitrogen (N) fixation (SNF) occurs in specialized organs called nodules after successful interactions between legume hosts and rhizobia. Within nodule cells, N-fixing rhizobia are surrounded by plant-derived symbiosome membranes, through which the exchange of nutrients and ammonium occurs between bacteria and the host legume. Phosphorus (P) is an essential macronutrient, and N2-fixing legumes have a higher requirement for P than legumes grown on mineral N. First, I investigated the impact of P deprivation on wild-type Medicago truncatula plants. My observations that plants had impaired SNF activity, reduced growth, and accumulated less phosphate in P-deficient tissues (leaves, roots and nodules) is consistent with those of similar previous studies. Galactolipids decreased with increase in phospholipids in all P-starved organs. Matrix-assisted laser desorption/ionization–mass spectrometry imaging (MALDI-MSI) of phosphatidylcholine (PC) species in nodules showed that under low P environments distributions of some PC species changed, indicating that membrane lipid remodeling during P stress is not uniform across the nodule. Secondly, a metabolomics study was carried out to test the alterations in the metabolic profile of the nodules in P-stress. GC-MS based untargeted metabolomics showed increased levels of amino acids and sugars and decline in amounts of organic acids in P deprived nodules. Subsequently, LC-MS/MS was used to quantify these compounds including phosphorylated metabolites in whole plant. My findings showed strong drop in levels of organic acids and phosphorylated compounds in P deprived leaves with moderate reduction in P deprived roots and nodules. Moreover, sugars and amino acids were elevated in whole plant under P deprivation. Finally, the last project of my thesis involved studying the response of PDIL3 (Phosphate Deficiency-Induced LncRNA-3) a long non-coding RNA (lncRNA) mutant under severe P stress. PDIL3 is known to regulate Pi-deficiency signaling and transport in M. truncatula (Wang et al., 2017). My results confirmed that in P starvation, pdil3 plants showed better shoot growth, accumulated more phosphate in shoots, had impaired SNF and less rhizobial occupancy in nodules than WT. Subsequently, MALDI–MS imaging was used to spatially map and compare the distribution of phosphatidylcholine (PC) species in nodules of pdil3 and WT in P-replete and P-depleted conditions. Several PC species showed changes in distributions in pdil3 nodules compared to WT in both P sufficient and P deprived conditions. These data suggest that PDIL3's role is not just suppression of the Pi transporter, but it may also influence P partitioning between shoots and nodulated roots, meriting further investigation.

This study consisted of two sheep grazed dryland pasture experiments. Experiment l compared sheep production from 3-year-old cocksfoot based pastures grown in combination with white, Caucasian, subterranean or balansa clover with a ryegrass-white clover pasture and a pure lucerne forage. Sheep liveweight gain per head from each pasture treatment and the pure lucerne stand was recorded in the 2006/07 and 2007/08 seasons. The cocksfoot-subterranean clover pasture provided equal (381 kg LW/ha in 2006) or higher (476 kg LW/ha in 2007) animal production in spring and gave the highest total animal production (646 kg LW/ha) averaged across years of the five grass based pastures. However, total annual liveweight production from lucerne was higher than any grass based pasture mainly due to superior animal production during summer when lucerne provided 42-85% higher animal production than any of the grass based pastures. In Experiment 2, the effect of stocking rate (8.3 (low) and 13.9 (high) ewes + twin lambs/ha) and time of closing in spring on lamb liveweight gain, pasture production and subterranean clover seedling populations was monitored over 2 years for a dryland cocksfoot-subterranean clover and ryegrass-subterranean clover pasture in Canterbury. In both years, twin lambs grew faster (g/head/d) in spring at low (327; 385) than high (253; 285) stocking rate but total liveweight gain/ha (kg/ha/d) was greater at high (7.26; 7.91) than low (5.43; 6.38) stocking rate. Ewes also gained 0.5 and 1.5 kg/head at the low stocking rate in 2006 and 2007 respectively but lost 0.2 kg/head in 2006 and gained 0.3 kg/head at high stocking rate in 2007. Mean subterranean clover seedling populations (per m²) measured in autumn after grazing treatments in the first spring were similar at both low (2850) and high (2500) stocking rate but declined with later closing dates in spring (3850, 2950, 2100 and 1700 at 2, 4, 6, 8 weeks after first visible flower). Seedling populations measured in autumn after grazing treatments in the second spring were also unaffected by stocking rate (low 1290, high 1190) but declined with later closing dates in spring (1470, 1320 and 940 at 3, 5 and 8 weeks after first flowering, respectively). The effect of stocking rate and closing dates in spring on pasture and clover production in the following autumn was similar to the effects on seedling numbers in both years. However, clover production in the following spring was unaffected by stocking rate or closing date in the previous year at the relatively high seedling populations generated by the treatments. This was presumably due to runner growth compensating for lower plant populations in pastures that were closed later in spring. Subterranean clover runner growth in spring may not compensate in a similar manner if seedling numbers in autumn fall below 500/m². Mean annual dry matter production from cocksfoot and ryegrass pastures grown with and without annual clovers pasture production ranged from 6.4 to 12.4 t DM/ha/y but stocking rate (8.3 vs. 13.9 ewes/ha) during spring did not affect annual pasture production. Pastures overdrilled with annual clovers yielded 23-45% more dry matter production than pastures grown without annual clovers. The study confirms the important role of subterranean clover in improving pasture production and liveweight gains of sheep in dryland cocksfoot and ryegrass pastures. Lowering stocking rate from 13.9 to 8.3 ewes/ha was a less effective method of increasing seed production of subterranean clover in dryland pastures although it did lead to increased liveweight gain per head.

The general goal of this research was to understand the agronomic and physiological changes of a lucerne crop in distinct physical radiation environments and to verify the potential of lucerne to grow under shaded conditions. To achieve this, the research was conducted in four main steps: (i) firstly, experimental data collection in the field using two artificial shade materials (shade cloth and wooden slats) under inigated and non-irrigated conditions; (ii) a second experiment with data collection in a typical temperate dryland agroforestry area under non-irrigated conditions; (iii) generation of a light interception sub-model suitable for shaded crops and (iv) a linkage between the light interception sub-model and a canopy photosynthesis model for agroforestry use. In experiments 1 and 2, lucerne crop was exposed to 6 different light regimes: full sunlight (FS), shade cloth (FS+CL), wooden slats (FS+SL), trees (T), trees+cloth (T +CL) and trees+slats (T+SL). The FS+SL structure produced a physical radiation environment (radiation transmission, radiation periodicity and spectral composition) that was similar to that observed in the agroforestry site (f). The mean annual photosynthetic photon flux density (PPFD) was 41 % under the FS+CL, 44% under FS+SL and 48% under T compared with FS in clear sky conditions. Plants were exposed to an intermittent (sun/shade) regime under both FS+SL and T, whereas under FS+CL the shaded light regime was continuous. The red to far-red (RIFR) ratio measured during the shade period under the slats was 0.74 and under the trees was 0.64. However, R/FR ratio increased to 1.26 and 1.23 during the illuminated period under FS+SL and T, respectively, and these were equivalent to the ratio of 1.28 observed under the FS+CL and 1.31 in FS. The radiation use efficiency (RUE) of shoots increased under the 5 shaded treatments compared with full sunlight. The pattern of radiation interception was unchanged by radiation flux, periodicity and spectral composition and all treatments had a mean extinction coefficient of 0.82. However, the magnitude of the decrease in canopy growth was less than those in PPFD transmissivity. The mean lucerne annual dry matter (DM) yield was 17.5 t ha⁻¹ in FS and 10 t ha⁻¹ under the FS+CL, FS+SL and T regimes. This declined to 3.4 t DM ha⁻¹ under T+CL (22% PPFD transmissvity) and 4.1 t DM ha⁻¹ under T+SL (23% transmissivity). A similar pattern of response was observed for leaf net photosynthesis (Pn) rates under the shade treatments compared with full sun. In addition, spectral changes observed under the trees and slats affected plant motphology by increasing the number of long stems, stem height and internode length compared with full sunlight. Thus, there were two main explanations for the increase in RUE under shade compared with full sun: (i) preferential partition of assimilates to shoot rather than root growth and/or (ii) leaves under shade were still operating at an efficient part of the photosynthetic light curve. The changes proposed for the canopy Pn model were appropriate to simulate the radiation environment of an agroforestry system. However, the model underestimated DM yields under the continuous and intermittent shade regimes. These were considered to be mainly associated with plant factors, such as overestimation in maintenance respiration and partitioning between shoots and roots in shade and the intermittency light effect on leaf Pn rates. Further investigation in these topics must be addressed to accurately predict crop yield in agroforestry areas. Overall, the lucerne crop responded typically as a sun-adapted plant under shade. It was concluded that lucerne yield potential to grow under intermediate shade was superior to most of C3 pastures previously promoted in the literature.

The annual and seasonal water use efficiency of six pasture combinations were calculated from the ‘MaxClover’ Grazing Experiment at Lincoln University. Pastures have been established for six years and are grazed by best management practices for each combination. Measurements for this study are from individual plots of four replicates of ryegrass (RG)/white clover (Wc), cocksfoot (CF)/Wc; CF/balansa (Bal) clover; CF/Caucasian (Cc) clover; CF/subterranean (Sub) clover or lucerne. Water extraction measurements showed soils for all dryland pastures had a similar plant available water content of 280±19.8 mm. Dry matter measurements of yield, botanical composition and herbage quality were assessed from 1 July 2008 until 30 June 2009. Lucerne had the highest annual yield of 14260 kg DM/ha/y followed by the CF/Sub at 9390 kg DM/ha/y and the other grass based pastures at ≤ 6900 kg DM/ha/y. All pastures used about 670±24.4 mm/y of water for growth. Lucerne had the highest annual water use efficiency (WUE) of 21 kg DM/ha/mm/y of water used (total yield/total WU). The WUE of CF/Sub was the second highest at 15 kg DM/ha/mm/y, and the lowest was CF/Wc at 9 kg DM/ha/mm/y. The CF/Sub pastures had the highest total legume content of all grass based pastures at 21% and as a consequence had the highest annual nitrogen yield of 190 kg N/ha. This was lower than the monoculture of lucerne (470 kg N/ha). Ryegrass/white clover had the highest total weed component in all pastures of 61%. For dryland farmers spring is vital for animal production when soil temperatures are rising and moisture levels are high. The water use efficiency at this time is important to maximize pasture production. In spring lucerne produced 8730 kg DM/ha, which was the highest dry matter yield of all pastures. The CF/Sub produced the second highest yield of 6100 kg/DM/ha. When calculated against thermal time, CF/Sub grew 5.9 kg DM/ºCd compared with lucerne at 4.9 kg DM/ºCd. The higher DM yield from lucerne was from an extra 400 ºCd of growth. The highest seasonal WUE of all pastures occurred in the spring growing period. Linear regressions forced through the origin, showed lucerne (1/7/08-4/12/08) had a WUE of 30 kg DM/ha/mm (R2=0.98). Of the grass based pastures, CF/Sub produced 18 kg DM/ha/mm (R2=0.98) from 1/7 to 10/11/08 from 270 mm of water used. The lowest spring WUE was 13.5 kg DM/ha/mm by CF/Bal pastures which was comparable to the 14.3±1.42 kg DM/ha/mm WUE of CF/Wc, CF/Cc and RG/Wc pastures. During the spring, CF/Sub clover had the highest spring legume component of the grass based pastures at 42% and produced 120 kg N/ha. This was lower than the 288 kg N/ha from the monoculture of lucerne. Sub clover was the most successful clover which persisted with the cocksfoot. Based on the results from this study dryland farmers should be encouraged to maximize the potential of lucerne on farm, use cocksfoot as the main grass species for persistence, rather than perennial ryegrass, and use subterranean clover as the main legume species in cocksfoot based pastures. By increasing the proportion of legume grown the water use efficiency of a pasture can be improved. When pastures are nitrogen deficient the use of inorganic nitrogen may also improve pasture yields particularly in spring.

Crop rotation may be used to prevent the continuous selection of particular weed species adapted to one crop type. This might be useful for weed management, economy in herbicide applications and promoting biodiversity. Common simple crop sequences might be diversified by introducing perennial forage crops. Impacts of such perennial crops on weeds were studied with four approaches : 1) Large-scale weed surveys in 632 fields in western France showed that weed species composition differed most strongly between perennial alfalfa crops and annual crops. Comparisons of fields before, during and after perennial alfalfa suggested that community composition varies in a cyclic way during such crop rotations. Several weed species problematic in annual crops were suppressed during and after perennial crops, but the appearance of other species led to equal or even higher plant diversities. 2) A 3-year field experiment with contrasting crop management options allowed an investigation of the underlying mechanisms for this: The absence of soil tillage reduced weed emergence but increased the survival of established plants. The permanent vegetation cover and frequent hay cuttings reduced weed growth, plant survival and seed production. 3) Greenhouse experiments testing the regrowth ability of individual plants after cutting showed strong differences between species and functional groups. An two-factorial experiment suggested that the negative impacts of cutting and competition on weed growth were mainly additive. 4) Special measurements of weed seed predation in the field experiment showed positive correlations with vegetation cover, indicating that this ecosystem service may be particularly fostered by perennial crops. Consistent preferences of seed predators for certain weed species indicates that seed predation may be another cause of the observed weed community shifts.

Aphanomyces euteiches est un oomycète parasite racinaire des légumineuses causant des pertes de rendement récurrentes. La paroi d'A.euteiches contient 10% de N-acétylglucosamine (NAG) sous la forme de chitosaccharides non cristallins, associés aux glucanes pariétaux. Afin de pouvoir étudier leur activité biologique, un bioessai d'élicitation du système racinaire de la plante hôte Medicago truncatula a été mis au point en utilisant une préparation de fragments de chitine comme éliciteur témoin. La purification de fractions de parois hydrolysées, enrichies en NAG, a donné des fragments de glycanes composés de glucose et de NAG. Ces hétéropolymères présentent une structure nouvelle jamais décrite à ce jour. Le bioessai d'élicitation racinaire a révélé une activité biologique des fractions de paroi différente de celle des fragments de chitine chez M.truncatula. De façon intéressante, l'une des fractions induit des oscillations calciques nucléaires dans les cellules épidermiques de cultures de racines de M.truncatula, qui sont différentes de la réponse provoquée par des chitotétramères purs.

The general goal of this research was to understand the agronomic and physiological changes of a lucerne crop in distinct physical radiation environments and to verify the potential of lucerne to grow under shaded conditions. To achieve this, the research was conducted in four main steps: (i) firstly, experimental data collection in the field using two artificial shade materials (shade cloth and wooden slats) under inigated and non-irrigated conditions; (ii) a second experiment with data collection in a typical temperate dryland agroforestry area under non-irrigated conditions; (iii) generation of a light interception sub-model suitable for shaded crops and (iv) a linkage between the light interception sub-model and a canopy photosynthesis model for agroforestry use. In experiments 1 and 2, lucerne crop was exposed to 6 different light regimes: full sunlight (FS), shade cloth (FS+CL), wooden slats (FS+SL), trees (T), trees+cloth (T +CL) and trees+slats (T+SL). The FS+SL structure produced a physical radiation environment (radiation transmission, radiation periodicity and spectral composition) that was similar to that observed in the agroforestry site (f). The mean annual photosynthetic photon flux density (PPFD) was 41 % under the FS+CL, 44% under FS+SL and 48% under T compared with FS in clear sky conditions. Plants were exposed to an intermittent (sun/shade) regime under both FS+SL and T, whereas under FS+CL the shaded light regime was continuous. The red to far-red (RIFR) ratio measured during the shade period under the slats was 0.74 and under the trees was 0.64. However, R/FR ratio increased to 1.26 and 1.23 during the illuminated period under FS+SL and T, respectively, and these were equivalent to the ratio of 1.28 observed under the FS+CL and 1.31 in FS. The radiation use efficiency (RUE) of shoots increased under the 5 shaded treatments compared with full sunlight. The pattern of radiation interception was unchanged by radiation flux, periodicity and spectral composition and all treatments had a mean extinction coefficient of 0.82. However, the magnitude of the decrease in canopy growth was less than those in PPFD transmissivity. The mean lucerne annual dry matter (DM) yield was 17.5 t ha⁻¹ in FS and 10 t ha⁻¹ under the FS+CL, FS+SL and T regimes. This declined to 3.4 t DM ha⁻¹ under T+CL (22% PPFD transmissvity) and 4.1 t DM ha⁻¹ under T+SL (23% transmissivity). A similar pattern of response was observed for leaf net photosynthesis (Pn) rates under the shade treatments compared with full sun. In addition, spectral changes observed under the trees and slats affected plant motphology by increasing the number of long stems, stem height and internode length compared with full sunlight. Thus, there were two main explanations for the increase in RUE under shade compared with full sun: (i) preferential partition of assimilates to shoot rather than root growth and/or (ii) leaves under shade were still operating at an efficient part of the photosynthetic light curve. The changes proposed for the canopy Pn model were appropriate to simulate the radiation environment of an agroforestry system. However, the model underestimated DM yields under the continuous and intermittent shade regimes. These were considered to be mainly associated with plant factors, such as overestimation in maintenance respiration and partitioning between shoots and roots in shade and the intermittency light effect on leaf Pn rates. Further investigation in these topics must be addressed to accurately predict crop yield in agroforestry areas. Overall, the lucerne crop responded typically as a sun-adapted plant under shade. It was concluded that lucerne yield potential to grow under intermediate shade was superior to most of C3 pastures previously promoted in the literature.

[Truncated abstract] Industrial processing to refine alumina from bauxite ore produces millions of tonnes of refining residue each year in Australia. Revegetation of bauxite residue sand (BRS) is problematic for a number of reasons. Harsh chemical conditions caused by residual NaOH from ore digestion mean plants must overcome extremely high pH (initially >12), saline and sodic conditions. At such high pH, manganese (Mn) is rapidly oxidised from Mn2+ to Mn4+. Plants can take up only Mn2+. Thus, Mn deficiency is common in plants used for direct BRS revegetation, and broadcast Mn fertilisers have low residual value. Added to this, physical conditions of low water-holding capacity and a highly compactable structure make BRS unfavourable for productive plant growth without constant and large inputs of water as well as Mn. However, environmental regulations stipulate that the residue disposal area at Pinjarra, Western Australia, be revegetated to conform with surrounding land uses. The major land use of the area is pasture for grazing stock. Hence, pasture revegetation with minimum requirement for fertiliser and water application is desirable. This thesis investigates a number of avenues with potential for maintaining a productive pasture system on BRS whilst reducing the current level of Mn fertiliser and irrigation input. Emphasis was placed on elucidation of chemical and physical factors affecting Mn availability to plants in BRS

Arthropod species that have the potential to damage crops are food resources for communities of predators and parasitoids. From an agronomic perspective these species are pests and biocontrol agents respectively, and the relationships between them can be important determinants of crop yield and quality. The impact of biocontrol agents on pest populations may depend on the availability of other food resources in the agroecosystem. A scarcity of such resources may limit biological control and altering agroecosystem management to alleviate this limitation could contribute to pest management. This is a tactic of ‘conservation biological control’ and includes the provision of flowers for species that consume prey as larvae but require floral resources in their adult stage. The use of flowers for pest management requires an understanding of the interactions between the flowers, pests, biocontrol agents and non-target species. Without this, attempts to enhance biological control might be ineffective or detrimental. This thesis develops our understanding in two areas which have received relatively little attention: the role of flowers in biological control by true omnivores, and the implications of flower use by fourth-trophic-level life-history omnivores. The species studied were the lacewing Micromus tasmaniae and its parasitoid Anacharis zealandica. Buckwheat flowers Fagopyrum esculentum provided floral resources and aphids Acyrthosiphon pisum served as prey. Laboratory experiments with M. tasmaniae demonstrated that although prey were required for reproduction, providing flowers increased survival and oviposition when prey abundance was low. Flowers also decreased prey consumption by the adult lacewings. These experiments therefore revealed the potential for flowers to either enhance or disrupt biological control by M. tasmaniae. Adult M. tasmaniae were collected from a crop containing a strip of flowers. Analyses to determine the presence of prey and pollen in their digestive tracts suggested that predation was more frequent than foraging in flowers. It was concluded that the flower strip probably did not affect biological control by lacewings in that field, but flowers could be significant in other situations. The lifetime fecundity of A. zealandica was greatly increased by the presence of flowers in the laboratory. Providing flowers therefore has the potential to increase parasitism of M. tasmaniae and so disrupt biological control. A. zealandica was also studied in a crop containing a flower strip. Rubidium-marking was used to investigate nectar-feeding and dispersal from the flowers. In addition, the parasitoids’ sugar compositions were determined by HPLC and used to infer feeding histories. Although further work is required to develop the use of these techniques in this system, the results suggested that A. zealandica did not exploit the flower strip. The sugar profiles suggested that honeydew had been consumed by many of the parasitoids. A simulation model was developed to explore the dynamics of aphid, lacewing and parasitoid populations with and without flowers. This suggested that if M. tasmaniae and A. zealandica responded to flowers as in the laboratory, flowers would only have a small effect on biological control within a single period of a lucerne cutting cycle. When parasitoids were present, the direct beneficial effect of flowers on the lacewing population was outweighed by increased parasitism, reducing the potential for biological control in future crops. The results presented in this thesis exemplify the complex interactions that may occur as a consequence of providing floral resources in agroecosystems and re-affirm the need for agroecology to inform the development of sustainable pest management techniques.