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Neurochemical biomarkers to evaluate mercury toxicity in minkBasu, Niladri January 2005 (has links)
Mercury (Hg) is a toxicant of global concern, but few strategies exist to evaluate its biological effects on the ecosystem. Piscivorous wildlife, such as mink (Mustela vison), are particularly at risk because they can bioaccumulate Hg to concentrations known to impair neurological systems. Given that biochemical changes in the brain precede functional and structural impairments, I evaluated the use of neurochemicals as early-warning biomarkers of Hg toxicity in mink. Initial studies demonstrated that neurochemical biomarkers could be measured from mink carcasses collected from the field, as long as factors such as storage temperature and freeze thaw cycles were accounted for. To determine if Hg could directly impair neurochemicals in mink, an in vitro study demonstrated that Hg (HgCl2 and MeHg) could inhibit radioligand binding to the muscarinic cholinergic (mACh) receptor in the cerebellum and cerebral cortex regions of the brain. By analyzing whole brains collected from wild mink, it was demonstrated that a significant positive correlation existed between concentrations of brain Hg and levels of mACh receptors. These field observations were confirmed by results from a controlled methyl Hg (MeHg) feeding trial in captive mink, whereby sub-chronic exposure of mink to 0.5 - 2 ppm MeHg (ecologically relevant levels) resulted in significant increases in acetylcholinesterase activity and mACh receptor levels in specific brain regions. Collectively, these results demonstrated that exposure of mink to environmentally realistic concentrations of Hg can be related to alterations in neurochemicals at multiple tiers of biological organization. Given the importance of a functional nervous system in wildlife health, the physiological and ecological significance of these findings need further exploration. The results demonstrate that neurochemical approaches may be novel biomarkers to assess the ecotoxicology of Hg, and by extension, other pollutants o
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Evaluation of methodology for mercury exposure assessment with field and laboratory studiesLegrand, Melissa January 2005 (has links)
The threat of environmental mercury (Hg), particularly methylmercury (MeHg), exposure to the health of humans has been well documented. Thus, it is important to monitor exposure and body burden for public health protection. The first objective of this thesis was to characterize the risk of Hg exposure in two Canadian coastal communities: Grand Marian (n = 91) and St. Andrews/St. Stephen (n = 52), New Brunswick, Canada, using dietary questionnaires and hair analysis. Average Hg intakes and body burden were below the most conservative guidelines. We attributed these results to the low Hg concentrations found in the species commonly consumed: haddock, canned tuna, lobster and pollock (all below 0.2 mg/kg). The analytical method employed to determine Hg in hair, cold vapor atomic absorption (CV-AAS), required a bundle of 100-150 hair strands and involved lengthy chemical digestion procedures which reduce throughput. Direct solid introduction techniques minimize these weaknesses. Our second and third objectives were to evaluate two such methods: (1) combustion, gold amalgamation, atomic absorption spectrometry (C-GA-AAS) and (2) laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for measuring total Hg in single hair strands. Hair samples with a wide range of Hg exposure were obtained from communities. A 1:1 relationship was observed between C-GA-AAS and the established CV-AAS for analysis of 1-cm hair segments. Additionally, the average relative standard deviation (RSD) of Hg between hair strands within an individual was 6.5 +/- 2.8%, thus justifying the use of a single hair strand for biomonitoring. With a limit of quantification of 0.10 ng of total Hg, a single hair strand with average weight of 0.5 mg and Hg concentrations of 0.2 mg/kg can be measured routinely. Using LA-ICP-MS, we showed that a single laser shot can sample hair material within 50 mum along a single hair strand which is equivalent to less than one day of
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Neurochemical biomarkers to evaluate mercury toxicity in minkBasu, Niladri January 2005 (has links)
No description available.
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Evaluation of methodology for mercury exposure assessment with field and laboratory studiesLegrand, Melissa January 2005 (has links)
No description available.
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Total Mercury in Stranded Marine Mammals from the Oregon and Southern Washington CoastsWintle, Nathan J. 01 January 2011 (has links)
Muscle samples from 105 marine mammals stranded along the Oregon-Washington coasts (2002-2009) were tested for levels of total mercury by Cold Vapor Atomic Fluorescence Spectrometry. The total mercury present is predominantly in the form of highly toxic methylmercury. After normalizing muscle tissue to 75% water weight, due to variance in water content, Steller sea lions (Eumetopias jubatus) and northern elephant seals (Mirounga angustirostris) exhibited the highest mean concentrations of total mercury followed by harbor seals (Phoca vitulina), harbor porpoises (Phocoena phocoena), and California sea lions (Zalophus californianus); 0.34 ± 0.278, 0.34 ± 0.485, 0.21 ± 0.216, 0.17 ± 0.169 and 0.15 ± 0.126 mg/kg normalized weight, respectively. Mean normalized values demonstrated limited muscle methylmercury accumulation in these species in the Pacific Northwest. Normalizing muscle mercury concentrations eliminated variability from desiccation, and allowed for a clearer indication of the amount of mercury the animal accumulated before stranding. However, actual wet weight concentrations in some of the stranded carcasses were high enough to pose a risk to scavengers.
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