Microhistological analysis is a highly effective microscopic technique of determining botanical composition of animal diets by visual recognition of fecal plant fragments, and has been widely used in range studies. The objective of this study was to use microhistological techniques to predict the botanical composition of domesticated horse diets when grazing mixed cool-season pasture. Samples of tall fescue [Lolium arundinaceum (Schreb.) S.J. Darbyshire], Kentucky bluegrass (Poa pratensis L.) and orchardgrass (Dactylis glomerata L.) were evaluated for microscopically unique characteristics. Cool season pasture grazing studies were conducted in October 2006 and May 2007 in Lexington, KY. Eight thoroughbred mares were placed in individual paddocks of varying botanical compositions to graze for six days. For each percent increase of tall fescue or orchardgrass in the paddock, there was a corresponding increase of 0.44% and 0.42%, respectively, in the diet. In conclusion, microhistological analysis is a useful tool for determining botanical composition of horse diets when grazing cool season grass pastures.

Master of Science / Department of Animal Sciences and Industry / K C Olson / Eight native tallgrass pastures infested with sericea lespedeza (initial basal frequency = 2.9 ± 2.74%) were grazed by yearling beef steers (n = 279 / yr; 1.1 ha / steer) from 15 April to 15 July for 2 grazing seasons. Subsequent to steer grazing, mature ewes (n = 813 / yr) were allowed to graze 4 of the 8 pastures (0.15 ha / ewe) from 31 July to 1 October. Remaining pastures were rested until the subsequent April. Animals were allocated randomly to pastures annually; grazing treatments remained fixed for the 2-yr experiment. Four permanent 100-m transects were laid out in a north-south gradient in each pasture. Beginning 1 May, steer diet composition was monitored by collecting 5 fresh fecal pats along each transect at 2-wk intervals until steers were removed on 15 July. Fecal pats were dried and ground individually and then composited by weight within transect. Twenty-five mature ewes from each pasture were randomly selected to monitor diet composition. On 15 August and 15 September, ewes were gathered and restrained; fecal grab samples were then collected from the individuals selected for diet composition analysis. Microhistological analysis was conducted on steer fecal composites and fecal samples from individual ewes, using pure samples of 17 predominant grass, forb, and browse species from the experimental site as reference standards. Fecal and standard samples were prepared for microhistological analysis and viewed using a compound microscope at 100x magnification. Botanical composition of pastures was measured in October and compared with botanical composition of yearling beef steer and mature ewe diets. Diet selection exercised by yearling steer and mature ewes was evaluated using Kulcyznski’s Similarity Index. Proportions of bare soil, litter cover, and total basal vegetation cover did not differ (P ≥ 0.38) between pastures for either steer or sheep diet evaluations. Similarly, basal cover of the 17 plants selected as microhistological standards did not differ (P ≥ 0.07) between pastures for either steer or sheep diet evaluations. The proportions of total graminoids and total forbs and forb-like plants in the diets of grazing steers were not different (P = 0.37) between sampling periods and were interpreted to indicate steer diets were strongly dominated by graminoids (≥ 88.4% of diets). Yearling beef steers consistently exhibited strong preference (i.e., ≤ 6% similarity with pasture composition) for Bouteloua gracilis, Bouteloua dactyloides, Dalea purpurea, and Liatris punctata, whereas they strongly avoided Lespedeza cuneata and Symphyotrichum ericoides. The proportions of total graminoids and total forbs and forb-like plants in the diets of grazing ewes were not different (P = 0.67) between sampling periods and were interpreted to indicate that mature ewes selected consistent proportions of grasses and forbs over time (average = 58 and 42% of diets for grasses and forbs, respectively). Mature ewes consistently exhibited strong preference (i.e., ≤ 10% similarity with pasture composition) for Bouteloua gracilis, Bouteloua dactyloides, Dalea purpurea, Liatris punctata, Vernonia baldwinii, and Ambrosia artemisiifolia. None of the 17 microhistological reference standards were consistently avoided by ewes.

Belize has been identified as an important location for Antillean manatees (Trichechus manatus manatus), harboring the highest known population density of this subspecies. Information about their dietary habit is important in determining habitat requirements and aiding in conservation efforts. The main objective of this study was to identify the key plant species consumed by manatees in Belize and to establish differences in diet based on location, sex, size classification, and season. Samples were collected from two different locations within Belize where manatees are known to aggregate: Southern Lagoon and the Drowned Cayes off of Belize City. The contents of thirteen mouth, six digestive tract (stomach, duodenum and colon), and 124 fecal samples were analyzed. Five species of seagrasses (Halodule wrightii, Thalassia testudinum, Ruppia maritima, Syringodium filiforme, and Halophila sp.) made up the highest percentage of plants consumed; undigested rhizome was most prevalent. A vascular plant, the red mangrove (Rhizophora mangle), was identified as an important food item of manatees in Belize. Algae (Chara sp., Lyngbia sp., and Ulvasp.) and invertebrates (diatoms and sponges) were represented as well. These items are comparable to other reports of manatee diets in areas near Belize and areas with similar habitat. Variation in the percentage of seagrass, mangrove, and algae consumption was analyzed as a 4-factor factorial Analysis of Variance (ANOVA) with main effects and interactions for locality (Southern Lagoon and the Drowned Cayes), sex, size classification (>245 cm & ˂245 cm), and season (December – May and June – November). Sex and season did not influence diet composition; differences for locality and size classification were observed. Seagrass was more often consumed in Southern Lagoon (P = 0.03), whereas mangroves and algae were more prevalent in the Drowned Cayes (P = 0.03). No differences among size classifications in consumption of seagrasses or mangroves were observed. A significantly higher number of samples from adults (>245 cm) than juveniles (˂245 cm) contained algae (P = 0.04). This is most likely attributed to inexperience in foraging. Findings from these results suggest that diet composition analysis can be used to interpret Antillean manatee habitat and resource utilization and can aid in the conservation of this endangered species.