DNA content varies significantly over land plants, and is known to correlate with various aspects of plant form and function. In the present study, two measures of DNA content were examined in taxa across the land plant phylogeny: genome size (or C-value) and endopolyploidy (or endoreduplication index, EI). Additionally, the relationships between DNA content and various morphological and ecological traits were assessed. DNA content was determined for 64 liverwort species from 33 families. There was a large range in 1C-values from 0.27 to 20.46 pg, but no endopolyploidy was observed. There was no correlation between genome size and breeding system (monoecy vs. dioecy). Genome size and degree of endopolyploidy were determined for 74 moss species from 21 families. Genome sizes were constrained in this group, with 1C-values ranging from 0.25 to 1.21 pg. Endopolyploidy was high in all species except from the Sphagnaceae. Additionally, 1C-value was negatively correlated with desiccation tolerance but was not correlated with breeding system. DNA content variation was determined in 31 monilophyte (fern) species (including three horsetails) and 6 lycophyte (clubmoss) species. There was a wide range in 1C-values from 2.79 to 26.90 pg, and there was no indication of endopolyploidy in any of the species. Multivariate analyses were used to explore the relative contribution of traits and phylogenetic placement to DNA content varation in 41 angiosperm species. Six measures of DNA content (2C-value, 1Cx-value, leaf EI, stem EI, petal EI and root EI) were assessed. Phylogeny explained more of the variation observed in the six measures of DNA content than 21 ecological and morphological traits. However, many of the traits were able to explain some of the variation in DNA content, both with and without phylogeny included as a covaraite. One trait that was consistently correlated with DNA content was the association with arbuscular mycorrhizal (AM) fungi. In a controlled experiment, the EI in root cells colonized by AM fungi significantly increased compared to non-mycorrhizal plants. This thesis increases our knowledge regarding the extent and significance of variation in DNA content in land plants.