Transcription is a major step in expression of genes of a given organism. Due to environmental constrains this step must be regulated in the favour of the sustenance and growth of the organism. Here comes the relevance of transcription factors, mostly proteins which regulate transcription. One such important group of transcription factors is the zinc finger proteins. It is well known that in eukaryotes the C2H2 zinc finger domain containing proteins are the largest group of transcription factors while in prokaryotes the largest group of transcription factors are represented by helix-turn-helix motif containing proteins. Till now only two C2H2 zinc finger domain proteins-Ros and Muc have been found in alpha proteobacteria which are also transcription factors. In eukaryotes the second largest group of zinc finger proteins have their zinc ion coordinated by four cysteine residues- the C4 zinc finger proteins. They make the nuclear hormone receptor superfamily of proteins. They have also been shown to act as transcription factors. But in eubacteria no such proteins have been described in details except an isolated report of crystal structure of a C-terminal zinc finger domain protein- Jann_2411 from Jannaschia sp. Though a lot of transcription factors have been described in mechanistic details in Escherichia coli and Bacillus subtilis, the list of well described mycobacterial transcription factors is short. Given this fact and the lack of any known zinc finger domain transcription factor in actinobacteria we wanted to see whether M. smegmatis genome also encode any homologue of Jann_2411 and if does whether they have ability to modulate transcription. To meet our aim we did BLASTP search against the genome of M. smegmatis using Jann_2411 as query. We found four C-terminal zinc finger domain proteins –Msmeg_0118. Msmeg_3613, Msmeg_3408 and Msmeg_1531, which we named as Mycobacterial single zinc finger protein (Mszfp) and numbered- Mszfp1, Mszfp2, Mszfp3 and Mszfp4, respectively. Mszfp1 and Mszfp2 were chosen for study as they were the top most hits. In this thesis:- Chapter1 introduces zinc finger proteins, transcription and several levels of control of transcription process in eubacteria. In chapter2 we characterised Mszfp1 biophysically and probed its secondary structure content and oligomeric state in the native and demetallated conditions. We have also shown that this conserved hypothetical protein is expressed throughout the growth phase of M. smegmatis, regulated by SigA and SigB. We have also showed that Mszfp1 is a DNA binding protein in the native state and the demetallated protein has altered DNA binding ability. It was noted that on over expression Mszfp1 affects colony morphology and biofilm forming ability, of M. smegmatis. In chapter3 the ability of Mszfp1 to bind to RNA polymerase of M. smegmatis has been explored. It was found that Mszfp1 can activate transcription by interacting with CTD/NTD of α subunit and domain 4 of σA like CRP on type II CRP activated promoter. In chapter4 similar to Mszfp1 the biophysical study of Mszfp2 has been carried out. It was found that Mszfp2 is also a predominantly alpha helical protein with oligomeric structure having DNA binding ability. Similar to Mszfp1 Mszfp2 on over expression changes the colony morphology. Chapter5 deals with the RNA polymerase binding ability of Mszfp2 and its ability to activate transcription by interacting with CTD/NTD of α subunit but not the σA. In chapter6 we have presented a glimpse of the possible biophysical properties of Mszfp3 and Mszfp4 and given a snapshot of distribution of homologues of Mszfps among other actinobacteria. We have also put forward a hypothesis about the origin of C4 and CCHC zinc finger domains. Chapter7 is the summary of the work embedded in the earlier chapters. In Appendix I is described the making of a bacteria (Bacillus licheniformis) driven heat engine. Appendix II describes an effort to study the visco-elastic properties of Mycobacterium smegmatis cells.