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<p>In the newspaper printing industry, offset is the dominating printing method and the use of multicolour printing has increased rapidly in newspapers during the last decade. The offset printing process relies on the assumption that an uniform film of ink of right thickness is transferred onto the printing areas. The quality of reproduction of colour images in offset printing is dependent on a number of parameters in a chain of steps and in the end it is the amount and the distribution of ink deposited on the substrate that create the sensation and thus the perceived colours. We identify three control points in the offset printing process and present methods for assessing the printing process quality in two of these points:</p><p>• Methods for determining if the printing plates carry the correct image</p><p>• Methods for determining the amount of ink deposited onto the newsprint</p><p>A new concept of colour impression is introduced as a measure of the amount of ink deposited on the newsprint. Two factors contribute to values of the colour impression, the halftone dot-size and ink density. Colour impression values are determined on gray-bars using a CCD-camera based system. Colour impression values can also be determined in an area containing an arbitrary combination of cyan magenta and yellow inks. The correct amount of ink is known either from a reference print or from prepress information. Thus, the deviation of the amount of ink can be determined that can be used as control value by a press operator or as input to a control system.</p><p>How a closed loop controller can be designed based on the colour impression values is also shown.</p><p>It is demonstrated that the methods developed can be used for off-line print quality monitoring and ink feed control, or preferably in an online system in a newspaper printing press.</p> / Report code: LiU-TEK-LIC-2005:02.

In the newspaper printing industry, offset is the dominating printing method and the use of multicolour printing has increased rapidly in newspapers during the last decade. The offset printing process relies on the assumption that an uniform film of ink of right thickness is transferred onto the printing areas. The quality of reproduction of colour images in offset printing is dependent on a number of parameters in a chain of steps and in the end it is the amount and the distribution of ink deposited on the substrate that create the sensation and thus the perceived colours. We identify three control points in the offset printing process and present methods for assessing the printing process quality in two of these points: • Methods for determining if the printing plates carry the correct image • Methods for determining the amount of ink deposited onto the newsprint A new concept of colour impression is introduced as a measure of the amount of ink deposited on the newsprint. Two factors contribute to values of the colour impression, the halftone dot-size and ink density. Colour impression values are determined on gray-bars using a CCD-camera based system. Colour impression values can also be determined in an area containing an arbitrary combination of cyan magenta and yellow inks. The correct amount of ink is known either from a reference print or from prepress information. Thus, the deviation of the amount of ink can be determined that can be used as control value by a press operator or as input to a control system. How a closed loop controller can be designed based on the colour impression values is also shown. It is demonstrated that the methods developed can be used for off-line print quality monitoring and ink feed control, or preferably in an online system in a newspaper printing press. / <p>Report code: LiU-TEK-LIC-2005:02</p>

In the newspaper printing industry, offset is the dominating printing method and the use of multicolour printing has increased rapidly in newspapers during the last decade. The offset printing process relies on the assumption that an uniform film of ink of right thickness is transferred onto the printing areas. The quality of reproduction of colour images in offset printing is dependent on a number of parameters in a chain of steps and in the end it is the amount and the distribution of ink deposited on the substrate that create the sensation and thus the perceived colours. We identify three control points in the offset printing process and present methods for assessing the printing process quality in two of these points: • Methods for determining if the printing plates carry the correct image • Methods for determining the amount of ink deposited onto the newsprint A new concept of colour impression is introduced as a measure of the amount of ink deposited on the newsprint. Two factors contribute to values of the colour impression, the halftone dot-size and ink density. Colour impression values are determined on gray-bars using a CCD-camera based system. Colour impression values can also be determined in an area containing an arbitrary combination of cyan magenta and yellow inks. The correct amount of ink is known either from a reference print or from prepress information. Thus, the deviation of the amount of ink can be determined that can be used as control value by a press operator or as input to a control system. How a closed loop controller can be designed based on the colour impression values is also shown. It is demonstrated that the methods developed can be used for off-line print quality monitoring and ink feed control, or preferably in an online system in a newspaper printing press. / <p>Report code: LiU-TEK-LIC-2005:02.</p>

Current single molecule localisation microscopy methods allow for multicolour imaging of macromolecules in cells, and for a degree quantification on molecule numbers in one colour. However, that has not yet been an attempt to develop tools capable of quantitative imaging with multiple colours in cells. This work addressed this challenge by designing linker peptides with chemospecific groups to allow attachment of activator and emitter dyes for STORM imaging, and a targeting module. The design ensured a stoichiometric ratio of targeting module to activator and emitter dyes. Peptides with HaloTag ligands attached were labelled with various activator and emitter pairs and used to label HaloTag fusions of S. pombe and mouse embryonic stem cells. These peptides were found to bind non-specifically to various areas of both cell types, and did not localise to HaloTag protein, whereas controls did. Another peptide was also labelled with activator-emitter pairs and attached to expressed anti-GFP and ant-mCherry nanobodies via native chemical ligation. The labelled anti-GFP nanobody was to demonstrate ensemble and single molecule imaging in S. pombe, as well as characterisation on single molecule surfaces in comparison to a conventional randomly labelled antibody. The stoichiometrically labelled nanobody had a more consistent number of photons detected per localisation, number of localisation per molecule and number of blinks per molecule, which implied that it could be more useful than randomly labelled nanobodies for counting experiments. It was also shown to be capable of specific laser activation for STORM imaging with both an Alexa405Cy5 and Cy3Cy5 pairs. These anti-GFP and anti-mCherry nanobodies and peptide linker are new tools for both counting and multicolour imaging in super-resolution, which could be widely applied to constructs that are already tagged with GFP or mCherry.