Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Lucie Kovářová Supervisor: Ing. Vladimír Kubíček, CSc. Title of Diploma Thesis: Multicomponent analysis This diploma thesis deals with the determination of concentrations of individual dyes in mixtures by multicomponent spectrophotometric analysis. The method was tested on selected dyes which were divided into two groups. The first group contains methyl orange, methyl red, orange II and phenol red. The second group consists of methylene blue, bromocresol green and thymol blue. Within each group mixtures were prepared containing combinations of two, three and four dyes in various ratio. Spectra of prepared mixtures were measured by diode array UV/VIS spectrophotometer. Absorbance values were read at wavelengths of absorption maxima of the individual dyes. Using the pseudoinverse matrix, concentration ratios of components were calculated from the absorbance values. The determination in determined and over determined systems was performed. On the basis of the results obtained by the method of analysis it is possible to determine the individual concentration of the multi-component dyes mixtures without prior separation. Keywords: spectrophotometry, multicomponent analysis,...

Multicomponent signals are complex stimuli directed to receptors of only single modality. Colourful ornaments of animals are multicomponent signals. In this thesis I present results of studies on the origin of coloration in turtles and squamate reptiles together with notes on relativistic view of the functionality of animal coloration. The results show that turtle coloration, which have been studied only marginally until now, is shaped by sexual selection. It is shown that turtles share mechanisms of coloration by vertical organization of different pigment cell types together with squamate reptiles. Turtles also produce colour by organization of collagen fibres which share trait with birds and mammals. Mechanisms of body coloration differ dramatically between closely related turtle species studied even though the individual constituting components are shared among these species. On the example of polymorphic lizards, it is shown that qualitative categorical difference between groups of individuals of the same population are maintained based on quantitative changes in pigment contents regulated by ancient loci shared by different species. The turtles and reptiles are valuable source of our knowledge on the evolution of multicomponent visual signalling due to their intriguing composition of skin....

The main goal of this dissertation was to create and verify own multicomponent tasks of interdisciplinary character based on analysis of types of tasks occured in international research. Newly created multicomponent learning tasks were classified by task type, solution form, cognitive demands and task variety. Tasks of the same form were ordered to pairs, their difficultness, vulnerability and rate of success were verified. Multicomponent learning tasks were assigned verbally or nonverbally (charts, tables or pictures). Closed jobs (ordering items, matching items, multiple choice questions, true/false questions) and open jobs (wide-answer items, short-answer items) were used in complex of tasks. Rate of success of individual tasks was processed by non-parmetric statistical Wilcoxon test based on form, gender and type of study. Research survey was complemented by pupils' self- reflection questionnaires and teachers'questionnaires about testing process. Key words Multicomponent learning task, taxonomy of learning task, scientific literacy, reading literacy, self-reflective questionnaire.

Learning exercises containing chemical calculations are necessary for complex understanding of some parts of chemical curriculum. This diploma thesis deals with creation and evaluation of worksheets containing multicomponent learning tasks closely connected to reality for pupils of elementary and high school. The theoretical part consists of the summary of present valid Czech curricular documents, mainly parts focused on chemical calculations and sustainable development. The main part of this thesis is creation of worksheets containing multicomponent learning tasks focused on different topics of chemistry which were chosen according to Framework Education Programmes. Created worksheets were verified and evaluated at high school. Based on evaluations of worksheets action research focused on problems during solving chemical calculations was conducted on elementary school.