The subject of the diploma thesis is a proposal of a community center in Žďár nad Sázavou. The proposal deals with the adaptation of the original building of the 1960s Creche and its reconstruction into a Community Center, which also includes a low-threshold center, a small crche, a social services headquarters building and a day care center for mentally disabled people. The work was elaborated in cooperation with the management of Žďár nad Sázavou and the city architect.

In the year 1903, the oldest existing Bohemian music society, the Tonkünstler-Wittwen-und-Waisen-Societät, founded in Prague in March 1803, finally ceased all its music activities; it continued its work just as a pension fund up to its final closing in 1930.

The topic of this diploma thesis is the suburban landscape, place and space of the Vrané nad Vltavou municipality located in close proximity to the capital city of Prague. The author approaches the suburban landscape as a specific type of landscape, which combines elements of urban and rural character. The author describes the manifestations of this landscape liminality using ethnographic methods and demonstrates the diversity of spatial structures with her own visual documentation, together with a depiction of the specificity of not only the researched landscape, but also the specificity of the suburb itself in the Czech context. Through the concept of the landscape applied in the socio-anthropological discourse, the author connects the material aspects of the natural environment with the social level of ideas, feelings, and experiences that everyone forms in contact with the landscape. In this sense, the landscape is never finished and neutral, but on the contrary, it is constantly negotiated and confirmed in the daily interactions of human and the landscape. The same holds true of the landscape, which is the subject of suburbanization processes changing its character. Therefore, on the one hand, the author depicts the character of the researched suburban landscape, on the other hand, she maps...

The objective of this diploma thesis was to create suggestions of biological excursions for the pupils of the second grade of elementary schools in Ústí nad Labem region. The thesis is divided into theoretical and practical part. In the theoretical part, excursion as the organisational form of education with the focus on the specifics of biological excursion including the educational goals and methods, is shortly introduced. In the following part, there is the characteristic of scientific disciplines and particular types of biological excursions. The practical part presents suggestions for three biological excursions. Each of the suggested subjects contains detailed manual for preparation and the process of excursion itself including the final part. Materials including the description of the route, information about the locality, worksheets including answers and additional information to each of the questions are also incorporated. Verification of chosen excursion in practice was conducted in June 2020 with pupils of class 7 of elementary school in Polepy. Keywords Excursion, biology, worksheets, Ústí nad Labem region

The goal of the thesis is an architectural study of Exposition Hall for the okapi, bongo and the hippos, including open-air concept. The pavilion has an organic, regular shape of drop, which is the entry point deformed inwards. The face is made of aluminum facade system. The interior of the pavilion evoking a rainforest environment with rooflights and pillars in the shape of trees. Around the pavilion to span the different paddocks lined visitor's tour, seats and view points.

Reactive oxygen species (ROS) such as H2O2 can damage cellular components and are formed as a byproduct of mitochondrial oxidative metabolism. Studies using the nematode C. elegans have found that increasing ROS during development or early adulthood can extend lifespan, while increasing ROS during later adulthood normally decreases lifespan. NADPH provides the reducing power for several cellular antioxidants and is synthesized in a two-step reaction from NAD+ with the first step being catalyzed by NAD kinase (NADK). In this study, the effects of knocking down C. elegans cytoplasmic NADK, nadk-1 globally or in a neuron or intestine-specific manner starting from early development on oxygen consumption and ROS levels were determined. Surprisingly, whole body knockdown of nadk-1 to decrease cytoplasmic NADPH levels decreased oxygen consumption and tert-butyl hydroperoxide-stimulated ROS levels, which was phenocopied by intestine-specific or neuron-specific knockdown. Thioredoxin reductase measurements following nadk-1 knockdown showed a trend toward increased activity.

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Coenzyme nicotinamide adenine dinucleotide (NAD, including the oxidized form-- NAD+ and reduced form--NADH) and the phosphorylated form--nicotinamide adenine dinucleotide phosphate (NADP, including NADP+ and NADPH) are two of the most important biological electron carriers. Most NAD(P) dependent redox enzymes show a preference of either NADP or NAD as an electron acceptor or donor depending on their unique metabolic roles. In biocatalysis, the low enzymatic activities with unnatural coenzymes have made it difficult to replace costly NADP with economically advantageous NAD or other biomimetic coenzyme for catalysis. This is a significant challenge that must be addressed should in vitro biocatalysis be a viable option for the practical production of low-value biocommodities (i.e., biohydrogen). There is a significant need to first address the coenzyme selectivity of the NADP-dependent dehydrogenases and evolve mutated enzymes that accept biomimetic coenzymes. This is a major focus of this dissertation. Establishment of efficient screening methods to identify beneficial mutants from an enzymatic library is the most challenging task of coenzyme engineering of dehydrogenases. To fine tune the coenzyme preference of dehydrogenases to allow economical hydrogen production, we developed a double-layer Petri-dish based screening method to identify positive mutant of the Moorella thermoacetica 6PGDH (Moth6PGDH) with a more than 4,278-fold reversal of coenzyme selectivity from NADP+ to NAD+. This method was also used to screen the thermostable mutant of a highly active glucose 6-phosphate dehydrogenase from the mesophilic host Zymomonas mobilis. The resulting best mutant Mut 4-1 showed a more than 124-fold improvement of half-life times at 60oC without compromising the specific activity. The screening method was further upgraded for the coenzyme engineering of Thermotaga maritima 6PGDH (Tm6PGDH) on the biomimetic coenzyme NMN+. Through six-rounds of directed evolution and screening, the best mutant showed a more than 50-fold improvement in catalytic efficiency on NMN+ and a more than 6-fold increased hydrogen productivity rate from 6-phosphogluconate and NMN+ compared to those of wild-type enzyme. Together, these results demonstrated the effectiveness of screening methods developed in this research for coenzyme engineering of NAD(P) dependent dehydrogenase and efficient use of the less costly coenzyme in ivSB based hydrogen production. / Ph. D.

NAD(P)H:Quinone oxidoreductase-1 (NQO1) has been implicated in the bioreductive activation of the clinically active anticancer drug Mitomycin C (MMC) and a polymorphic variant of NQO1 which lacks functional enzyme activity (NQO1*2) has been linked with poor survival in patients treated with MMC. The relationship between NQO1 activity and cellular response to MMC is however controversial and the aim of this study was to determine whether the response of bladder cancer patients to MMC can be forecast on the basis of NQO1*2 genotype status. Genomic DNA was extracted from formalin-fixed, paraffin-embedded tissue from 148 patients with low to intermediate grade (G1/G2) superficial (Ta/T1) bladder cancers and NQO1*2 genotype status determined by PCR-RFLP. NQO1*2 genotype status was retrospectively compared with clinical response to intravesical administered MMC with the primary end-point being time to first recurrence. NQO1 phenotype was determined by immunohistochemistry. Of the 148 patients genotyped, 85 (57.4%) were NQO1*1 (wild-type), 59 (39.8%) were NQO1*1/*2 (heterozygotes) and 4 (2.7%) were NQO1*2/*2. No NQO1 protein expression was detected in NQO1*2/*2 tumours. A broad spectrum of NQO1 protein expression existed in tumours genotyped as NQO1*1 and NQO1*1/*2 although tumours with NQO1*1 typically expressed higher NQO1 protein. A poor correlation existed between NQO1*2 genotype status and clinical response to MMC. The results of this retrospective study suggest that tailoring MMC therapy to individual patients with superficial bladder cancer on the basis of NQO1 genotype status is unlikely to be of clinical benefit.