of PhD Theses The Thesis is centred around the influence the Lazansky of Bukova aristocratic family had on the artistic environment in the Czech lands from the 16th to 20th century. On the background of their cultural-historical monograph, the author introduces the individual family member's construction and patronage activities, the development of their art collections and the wider context. Using the interdisciplinary research, the author con- fronts the results of his own work with the conclusions of previous researchers. He chal- lenges some of the earlier hypotheses for the Early Modern Age and draws from the untapped sources a wealth of information about the Lazansky family cultural activities in the modern history. He compares the resulting summary with the realizations of other aristocratic families in the Czech lands and in Central Europe. The author's efforts result is the first comprehensive view of the Lazansky of Bukova family in the wider context of cultural history, and in the rehabilitation of their role in artistic patronage. Keywords: Lazansky of Bukova, Marie Gabriela Lazansky, Prokop Lazansky, patronage, art collections, noble families, Manetin, Rabstejn nad Strelou, Chyse, Lubenec.

At the beginning of my thesis, the risk of floods in the territory of the Czech Republic is generally characterized which is followed by basic legal documents governing flood protection. The characteristics of the territory on which this thesis focuses is presented in the next section, which is describes in detail the administrative district of the municipality with extended powers (MEP) of Týn nad Vltavou, the basic description of its hydrological basin and waterways and, of course, the flood profile of the individual municipalities. The following section explains the term of flood, classification of floods and a detailed description of flood risks within detail the administrative district of the MEP of Týn nad Vltavou emphasizing the risk of formation a special flood. This is followed a list of complex activities and measures aimed at preventing and eliminating the threats to the health, lives and property of the citizens, society and the environment during floods. It also discusses planning in the field of flood protection and the classification of flood plans. Emphasis is placed on the documentation of flood protection of the administrative district of the MEP of Týn nad Vltavou that is associated with the activities of flood protection authorities. The section discusses in detail their activities during floods as well as when there are no floods. The function of flood committee of the MEP Týn nad Vltavou is mentioned detailing the description of activities of its individual members. The objectives of this thesis were aimed at finding out about the level of prepared flood protection documentation processed, analysing the activities of flood protection authorities and examining their knowledge about the issue of flooding. All the objectives were directed to the administrative district of the MEP Týn nad Vltavou and its flood protection authorities. Through a sociological survey among the flood protection authorities of the administrative district of the MEP Týn nad Vltavou that was conducted in the form of an anonymous questionnaire testing their knowledge in the field of flood protection. The results obtained were subjected to statistical evaluation, which, together with graphical representation, is of part of the thesis.

This Ph.D. thesis addresses the structure-function relationship of the multimeric oxidoreductase WrbA with the principal aim being the explanation of the unusual kinetics of this enzyme in molecular terms, and thus getting an insight about its physiological role in bacteria. WrbA is a multimeric enzyme with FMN as a co-factor, catalyzing the oxidation of NADH by a two electrons transfer. Structure and function analysis of WrbA places this enzyme between bacterial flavodoxins and eukaryotic oxidoreductases in terms of its evolutionary relationship. The kinetic activity of WrbA was studied under varying conditions such as temperature, pH etc, and its kinetic mechanism was evaluated from parameters KM and Vmax and confirmed by product inhibition pattern experiments. Crystallization and proteolytic experiments also underpin the functional importance of the multimeric nature of WrbA and aid the understanding of the physiological role of this enzyme in molecular terms.

O uso de dieta hiperlipídica com banha de porco em roedores induz obesidade, resistência à insulina e disfunção das células beta do pâncreas. Em diversos tecidos de animais tratados com dieta hiperlipídica já se observou aumento de expressão e/ou atividade da NAD(P)H oxidase, que pode estar envolvida em processos fisiopatológicos. O objetivo deste trabalho foi avaliar se a dieta hiperlipídica altera a expressão e/ou a atividade da NAD(P)H oxidase em ilhotas pancreáticas e se este fato pode estar associado às disfunções das células beta relatadas na literatura para este modelo animal. As ilhotas pancreáticas dos animais tratados com dieta apresentam maior secreção de insulina em alta glicose, maior metabolização da glicose, menos apoptose, menor expressão protéica de subunidades da enzima, menor produção de superóxido e não apresentam estresse oxidativo. O papel da enzima provavelmente se relaciona ao processo de secreção de insulina. A regulação de sua expressão e atividade deve estar relacionada à adaptação das ilhotas aos efeitos deletérios da dieta. / Feeding animals with high fat diet containing lard causes obesity, insulin resistance and dysfunction of pancreatic beta cells. High fat diet induces oxidative stress and modulates NAD(P)H oxidase expression and activity in many tissues. This enzyme may be involved in many pathophysiological processes. The objective of this study was to evaluate the action of high fat diet on NAD(P)H oxidase activity and expression and if this fact can be connected to the beta cell dysfunction reported in the literature on this animal model. In pancreatic islets of rats fed the high fat diet apoptosis was reduced, glucose metabolism increased, insulin secretion elevated at high glucose, protein expression of NAD(P)H oxidase subunits reduced and the superoxide production was diminished. There was no difference between the groups for oxidative stress markers. It is possible that the enzyme has a role in the process of insulin secretion. Probably the islets are regulating their activity and function to compensate the deleterious effect of lard.

Denna uppsats redovisar en studie i hur en söktjänst på Internet kan kompletteras, antingen via en mobilapplikation eller genom en responsiv webbplats, för att underlätta användning via en smartphone. Fokus för undersökningen ligger på Riksarkivets söktjänst Nationell ArkivDatabas (NAD) som för tillfället inte är anpassad till mobila enheter. Vårt mål är även att undersöka hur inställningen ser ut för applikationer och responsiva webbplatser bland användare samt hur dessa åsikter skiljer sig mellan olika användarmålgrupper. Tillsammans med information från tidigare forskning har en studie utförs för att undersöka för- och nackdelar mellan appar respektive responsiva webbplatser. I våra undersökningar har vi valt att använda oss av två datainsamlingsmetoder: en kvantitativ webbenkät och semistrukturerade kvalitativa intervjuer, som komplement till varandra. Totalt nio intervjuer har gjorts med tre personer ur varje Riksarkivets huvudmålgrupper. En webbenkät har även publicerats på Riksarkivets och Stockholms stadsarkivs webbplatser. / This paper reports a study in how a search service on the Internet can be completed either through a mobile application or through a responsive website, to facilitate use on a smartphone. The focus of the study is on the National Archives' search service National Archives Database (NAD), which is not currently adapted to mobile devices. Our aim is also to investigate how the attitude looks for applications and responsive websites among users and how these views differ between user groups. Together with data from previous research, a study was conducted to examine the pros and cons between apps and responsive websites. In our investigations we have chosen to use two methods of data collection: a quantitative web survey and semi-structured qualitative interviews, to complement each other. A total of nine interviews were conducted with three members from each of National Archives' main target groups. An online survey has also been published on the National Archives and Stockholm stadsarkivs websites.

The goal of this thesis is to describe establishment, development and forms of town twinnig. There is described development, definitions, benefits and forms of town twinning in the theoretical part. There are presented international organisations supporting town twinning too and also there is described a programm called "Town Twinning". Practical part is devoted to description of activities among choosen towns - Hradec Králové, Kostelec nad Orlicí, Vamberk and Albrechtice nad Orlicí. There are some proposals how to support tourism with these partner towns too.

Les travaux de cette thèse ont porté sur le développement de deux méthodes de modélisation des enzymes contenant des groupes prosthétiques de la famille des flavines.La première méthode, PredFace, permet de prédire la stéréochimie des produits d'une réaction catalysée par des enzymes contenant des groupes prosthétiques, en identifiant la face libre d'interaction avec les substrats. Le protocole mis en place pour cette méthode implique l'utilisation de huit complexes "sondes", obtenus par des opérations de symétrie à partir de l'état de transition de la réaction de transfert d'un atome d'hydrogène entre le nicotinamide et la lumiflavine. Ces complexes sont positionnés dans le site actif avec le groupe prosthétique comme référence et dans chaque cas l'énergie d'interaction protéine-ligand est évaluée par la fonction de score implémentée dans le logiciel de docking utilisé (AutoDock). L'énergie d'interaction la plus favorable permet d'identifier la face du groupe prosthétique accessible pour la réaction enzymatique dans le site actif. La méthode PredFace a été validée par l'analyse de l'ensemble des structures de la Protein Data Bank contenant des groupes prosthétiques de la famille des flavines (2170). Le protocole mis au point est très rapide (moins d'une minute), ce qui nous a permis de développer un site web afin de mettre cette méthode à la disposition de la communauté.La seconde méthode, ProsthDock, est une nouvelle méthode de docking basée sur le mécanisme d'une réaction enzymatique catalysée par un groupe prosthétique et guidée par la présence de ce groupe prosthétique dans le site actif de l'enzyme. Le développement de cette méthode a été motivé par le fait que les méthodes actuelles de docking, en présence de groupes prosthétiques, se révèlent incapables de produire des poses correctes pour des substrats, en accord avec les réactions enzymatiques. Afin de remédier à ce problème nous avons ajouté à la fonction de score classique un terme supplémentaire, qui rendra compte de l'interaction du ligand avec le groupe prosthétique. Dans un premier temps, nous avons construit un modèle simplifié du complexe NADH/FMN et calculé l'état de transition de la réaction de transfert d'hydrogène entre les deux partenaires. Des surfaces d'énergie potentielle pour cette réaction ont été calculées en variant la distance, l'angle et l'angle dièdre entre les deux réactifs. Un docking sous contrainte est ensuite réalisé et en fonction du positionnement de chaque pose de docking dans le site actif le terme supplémentaire de la fonction de score est calculé à partir des surfaces d'énergie potentielle, ce qui nous permet de modifier le classement des résultats de docking en favorisant les poses qui sont en accord avec la réaction enzymatique. / During this PhD thesis we developped two new molecular modeling methods applied to enzymes containing flavin-type prosthetic groups.The first method, PredFace, predicts the stereochemistry of products from a reaction catalyzed by enzymes containing prosthetic groups, by automatically identifying the solvent-exposed face of the prosthetic group. The protocol involves the use of eigth complexes as "probes", obtained by symmetry operations starting from the transition state of a hydrogen atom transfer reaction between nicotinamide and lumiflavin. These complexes are positioned in the binding site with the prosthetic group as reference and the energy of the protein-ligand interaction is evaluated by the scoring function implemented in the docking software (AutoDock). The most favorable interaction energy allows the identification of the prosthetic group face that is available for the enzymatic reaction in the binding site. The PredFace method has been validated by analyzing all the structures in the Protein Data Bank containing flavin-derived prosthetic groups (2170). This method is very fast (less than a minute), which allowed us to develop a web site open to the scientific community.The second method, ProsthDock, is a new mechanism-based molecular docking method guided by prosthetic groups present in the active sites of enzymes. The development of this method was motivated by the incapacity of the currently available docking methods to provide, in the presence of prosthetic groups, ligand conformations that are compatible with the enzymatic reactions. In this regard, we have added a new term to the classical scoring function, to take into account the interaction between ligand and prosthetic group. We have built a simplified model of the NADH/FMN complex and calculated the transition state of the hydrogen transfer reaction between the two partners. Potential energy surfaces have been calculated for this reaction by variating the angle, diedral angle and distance between the two reaction partners. A subsequent docking with constraints provides binding site conformations of the ligand for which the new term of the scoring function is calculated using the potential energy surfaces. This results in a new ranking of the docking poses, favoring those in agreement with the enzymatic reaction.

The theme of this diploma thesis deals with comparing technologies of reconstruction of the railway track No. 2 between railway stations Hranice na Morave and Hustopece nad Becvou in km 7,792 393 – 7,967 592 and in km 8,725 000 – 14,988 677. The aim of the thesis is to design several variants for the reconstruction of the substructure and superstructure, and then compare these variants. This thesis deals with technologies with removal of the track length and with technologies without removal of the track length. Part of the thesis is a description of used technologies and machines connected with them.

Master’s thesis is focused on problems associated with concept of suitable insurance portfolio of municipality Kobylá nad Vidnavkou. It includes risk analysis and suggestions of such an insurance portfolio that can help the municipality minimize impacts of the most serious risks through commercial insurance.

Between 2007 and 2016, there was a rescue archeological research done by the Institute of Archaeology of the CAS in the locality of Brandýs nad Labem-Vrábí at the site "U Vodojemu", where, above other, a large settlement from the Early Bronze Age was uncovered. Based on the ceramics, it is possible to classify it chronologically as the early period of the Únětice culture, typologically then, it is possible to associate the ceramics found with the post-classical phase of the Únětice culture. The discovery of the U-shaped ditch adjacent to the ground edge of the Hrušovský stream indicates that the settlement was fortified for a certain time, approximately at the beginning of the 2nd millennium BC. Based on the nature of the ditch filling, of the ceramic findings with analogies in the beginning of the tumulus culture, as well as of the carbon dating, it is obvious that the settlement served further on after the downfall of this fortification, too, and thus for another 100 years at least. In the context of another very numerous, contemporaneous findings in the cadaster of Brandýs nad Labem, it is visible that the site "U Vodojemu" used to be a part of a large settlement agglomeration. A detailed mapping of the Early Bronze Age findings from the district of the north-east part of Central Bohemia has...