Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: PharmDr. Ondřej Holas, PhD. Student: Daniela Širajová Title of thesis: Preparation of polymeric fluorescent nanoparticles Nanoparticles based on biodegradable polyesters are a widely used platform for targeted drug delivery and subsequent controlled release. The aim of this diploma thesis was to prepare and optimize the preparation of polymeric nanoparticles with a fluorescent dye as a model substance. The nanoprecipitation method was used to prepare the nanoparticles. Nanoparticles prepared from two types of PLGA polymers (COOH terminated and ester terminated) were evaluated and compared. The surfactant and stabilizer were used in various concentration ratios to optimize the preparation. The surfactant was sodium cholate at concentrations of 0.1%, 0.5%, 1%, 2% and 5%. The nanoparticles were stabilized with Pluronic F-127 poloxamer at concentrations of 0.1%, 0.5% and 1%. Nanoparticles were compared in terms of encapsulation efficiency, particle size and zeta potential. In a dissolution experiment, the amount of fluorescein released was evaluated and compared as a function of time (48 hours) with acid-terminated PLGA and sodium cholate at concentrations of 0.1% and 2%....

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: PharmDr. Ondřej Holas, PhD. Student: Katarína Kostíková Title of thesis: Study of the surfactants effect on polymer nanoparticle parameters The main aim of this Diploma thesis was to examine whether the use of various stabilizers has any effect on the resultant size of prepared nanoparticles and to find out which type of stabilizer is the most appropriate in terms of preparation of the nanoparticles of the necessary size, adequate polydispersity and steadiness. The preparation of nanoparticles was based on the use of sodium cholate as a surfactant and polymer PLGA (poly (lactid-co-glycolic acid)) consisting of monomers of lactic and glycolic acids. Nanoparticles were prepared by means of nanoprecipitation method. Sodium cholate solution has always been prepared in water in different concentration: 0.005%, 0.01%, 0.02%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 1%, 2%, and 5%. The following have been used as stabilizers: poloxamer 407, polyvinyl alcohol, cetrimonium-bromide (CTAB), and polysorbate 20, all of them in concentration of 0.1% and sorbitan-monostearate in concentration of 0.01%. Measurement of the size of created nanoparticles was performed using the device Zetasizer at the...