Little literature currently exists on naps in infancy, particularly in Early Childhood Education (ECE) settings. This study follows previous research by Stuart (2011) on children attending ECE Centres. The objective of the current study was to examine the architecture of naps in infants who were transitioning to attendance at an ECE Centre. Four males and one female aged between 4 and 11 months contributed to five case studies. Digital video recordings were made of participants napping in two settings: the home and the ECE Centre. Baseline recordings were made in the home only, and recordings were made in both settings as infants transitioned to the ECE Centre, and once they were deemed to be “settled” at the centre. The recordings were then coded to determine sleep states and amount of caregiver interaction. The results showed that all infants displayed a reaction to the transition to ECE attendance. However, the transition to the ECE Centre had a minimal effect on most infants’ home naps. Overall, naps were longer and more efficient at home than at the ECE Centre, and infants engaged in more Active Sleep than Quiet Sleep in both settings. Caregiver interaction during naps also differed between the settings. This is an important area of study as attendance at ECE Centres in New Zealand is increasing (Ministry of Education, 2011b), and as such, suggestions for future research have been made.

Thesis (M. Ed.)--University of Canterbury, 2009. / Typescript (photocopy). "February 2009." Includes bibliographical references (leaves 66-70). Also available via the World Wide Web.

Napping has been explored extensively as a means of counteracting the negative effects associated with shift work. A significant amount of this research has focused on the implementation of scheduled naps, with few studies considering flexible nap schemes. The current study therefore aimed to assess the effects of a flexible nap opportunity on the physiological, cognitive, performance, neurophysiological and subjective responses of a group of non shift workers over the course of a three-day simulated night shift regime. Additional foci were the effects of the nap condition on the extent of the circadian adaptation of the subjects to the irregular work schedule and the circadian-related influences associated with being awake during the night. 36 subjects – 18 males and 18 females – were recruited to participate in the current study. The data collection spanned twelve days, during which four, three-day long shift cycles were set up: three night shift cycles and one day shift cycle. During each night shift cycle, three separate experimental conditions were staggered, namely the nap condition, the no nap condition and a booster break condition (a collaborative study that completed the setup). The day shift served as a further comparison. Each cycle comprised of 12 subjects, which meant there were four subjects per condition during each cycle. The shifts were 8 hours in duration, with the no nap group following a standard break schedule evinced in industry. The three breaks taken during the shifts amounted to a total time of 1 hour. The nap group was afforded a 1 hour flexible nap opportunity between 00h00 and 03h00 with no other breaks. Therefore, both conditions had the same amount of work time. During the shifts, subjects performed two simple, low arousal tasks (beading and packing) and completed a test battery roughly every two hours which was comprised of physiological, performance, neurophysiological and subjective measures. It was found that the inclusion of the nap opportunity significantly improved output performance and response time during a low precision, modified Fitts tapping task over the course of three night shifts, relative to no napping. Physiologically, napping resulted in higher heart rate frequency measures by the end of the shifts, which were also accompanied by significant reductions in subjective sleepiness ratings during all iii the night shifts. The nap group’s responses in this case, did not differ significantly from those of the day shift. Both simple reaction time and memory performances improved as a result of the nap inclusion, but only during the third night shift. The majority of the measures included in the research also depicted the effects of the circadian rhythm, which was indicative of the pronounced effect that this natural biological down regulation has on performance during the night. Napping reduced the severity of these effects during beading performance and measures of subjective sleepiness. With regard to habituation, the nap opportunity also resulted in positive changes in the responses of beading performance, high precision response time, simple reaction time and both subjective sleepiness measures, relative to no napping. Sleep diary responses indicated that although sleep length and quality during the day were significantly reduced for both night-time conditions, recovery sleep (length and quality) for the nap group did not differ significantly from the no nap group. The findings of this research indicate that the inclusion of a flexible napping opportunity during the night shift had positive effects on some physiological, performance and subjective responses, and that this intervention is as beneficial as scheduled napping. Specifically, napping resulted in a significantly higher output during the beading task, relative to the no nap group despite the duration of work time being the same. As such the introduction of a flexible, self-selected nap opportunity is a practical, effective and individual-specific means of alleviating the negative effects of shift work, while improving certain performance parameters. Therefore, industries should consider its inclusion in their fatigue management programs. However, contextspecific considerations must be made, with regard work scheduling, individual differences and task demands when implementing such an intervention. This will ensure that its introduction will be well received and in time, lessen the health and work-related decrements associated with shift work.

Naps have been shown to benefit declarative memories in early childhood. This benefit has been associated with sleep spindles during the nap. However, whether young children’s naps and their accompanying physiology benefit other forms of declarative learning is unknown. Using a novel storybook task, we found performance was better following a nap compared to performance following an equivalent interval spent awake. Moreover, performance was better the following day if a nap followed learning. Further, change in post-nap performance was positively associated to the amount of time spent in slow wave sleep. This suggests that slow wave sleep in naps may support episodic memory consolidation in early childhood. Taken in conjunction with prior work, these results suggest that multiple features of brain physiology during naps may contribute to declarative memory processing in early childhood.

Limited research has been done on naps, particularly in early childhood education centres (ECECs). The present study followed a study by Torok (2009) with sleep-disturbed infants in ECECs. The objective of the current study was to examine the quality of naps in infants described as “typical sleepers” across two settings: the home and the early childhood education centre (ECEC). Two males and two females, ranging in age from 15- to 17-months contributed to four case studies. These were: i) an infant transitioning to the ECEC; ii) and iii) infants described as “settled” in an ECEC; and iv) an infant reported to have sleeping difficulties at home after the occurrence of a major earthquake. Observations from digital recordings were coded to determine sleep states and patterns. The findings across each case study were: i) naps varied in both settings during the infant’s transition to an ECEC but settled in both settings once the infant was “settled” at the ECEC; ii) naps tended to be consistent across both settings in the “typical sleepers” who were settled at the ECEC; and iii) naps at home were varied in the infant reported to have reacted to the earthquake while her naps at the ECEC were consistent. Overall findings suggested that total nap periods tended to be longer at home, sleep efficiency tended to be higher at the ECEC, and that participants tended to engage in more active sleep than quiet sleep. Caregiver presence was a major difference between the home and ECEC setting. This study demonstrated differences and similarities across both settings with infants described as “typical sleepers”. This is an important area due to the increasing number of infants attending ECECs (Statistics NZ, 2010). Several directions for future research have been presented.

Mu is a temperate bacteriophage which infects Escherichia coli and several other Gram negative enteric bacteria. It is an extraordinary phage in several respects and has carved a special niche for itself both as a genetic tool and a paradigm in phage biology, almost rivaling phage lambda. It is also a predator that has adapted its hunting skills well in order to have an extraordinarily wide host range. While phage Mu finds a mention in almost every genetics textbook for several of its unique and well-studied characteristics, there are a few aspects of its biology that are far from understood. In this thesis, light has been shed on one such less understood feature of Mu biology, namely its anti-restriction function. The enigmatic mom gene of bacteriophage Mu is the center of this thesis work. Bacteriophages, through their sheer number and versatility of attack tactics, constitute an overwhelming threat to bacteria in the natural environment. While it is not always possible to completely prevent the entry of foreign DNA into the cell, it is in the interest of the bacterium to tame the xenogeneic DNA, whose expression may have adverse effects on bacterial fitness. Bacterial nucleoid associated proteins (NAPs) participate in chromosome structuring and global transcriptional regulation. Besides this canonical role, they furnish the job of regulating xenogeneic DNA as well. NAPs are known to regulate the expression of toxin-antitoxin modules, pathogenicity islands and other horizontally transferred DNA and have a profound role in regulating transposon dynamics and the lifestyle of many phages. Chapter 1 introduces the role of bacterial NAPs in silencing foreign DNA, especially after the DNA establishes itself in the host. This thesis examines the role of a bacterial NAP namely Fis in fine-tuning an immune evasion function of bacteriophage Mu. A general introduction to phage Mu and its host expansion strategies, with special focus on its DNA modification function is also presented. Owing to the various immune evasion strategies, phages often have an upper hand on their hosts in the ongoing evolutionary arms race. One such strategy is DNA modification which bacteriophages have evolved as a means to protect their genomes from restriction enzymes of the host. While most phages employ the commonplace methylation modification for their anti-restriction function, phage Mu employs an unusual acetamido modification, catalysed by its protein Mom. Mom modified DNA is refractory to several restriction enzymes from different bacterial species. However, the modification is toxic to the host and thus mom expression needs to be precisely regulated to prevent untimely expression. A crowded multifactorial regulatory circuit has evolved to ensure the expression of mom without jeopardizing the welfare of the bacterial host. Chapter 2 uncovers a new player in mom regulation. The study shows that the bacterial chromatin architectural protein Fis is a transcriptional repressor of mom promoter and that Fis mediates its repressive effect by denying access to RNA polymerase at mom promoter. Two distinct roles of Fis have been known previously in Mu biology. In addition to bringing about the overall downregulation of transposition events and transcription of early genes of phage Mu, Fis also stimulates tail fiber flipping by aiding the activity of a site-specific recombinase. The present study thus presents a novel facet of Fis function in Mu biology. While the regulation of mom has been a matter of intense investigation over the past few decades, most biochemical and structural aspects of the Mom protein per se have remained mysterious owing to the difficulties in cloning this toxic gene. Chapter 3 describes the expression, purification and biophysical characterization of Mom. A variety of techniques show Mom to be folded and dimeric in solution. SPR studies with Mom indicate its high affinity binding to DNA. Chapter 4 deals with the attempts to identify the elusive co-factor of Mom. To begin with, the in vivo activity of Mom was demonstrated by employing a simple plasmid cleavage assay based on the resistance of Mom modified DNA to certain restriction endonucleases. A variety of disparate in silico structure prediction tools such as I-TASSER, Robetta and PHYRE indicate Mom to be related to the GCN5-related N-acetyltransferase superfamily. Mutation of residues deemed important from this analysis indeed abolished or reduced Mom activity in vivo, validating the bioinformatics based prediction and shed light on the possible active site of Mom. However, acetyltransferases are not known to transfer acetamido groups. It was also necessary to establish beyond doubt, the chemical structure of the Mom modified nucleoside. High resolution mass spectrometry data showed the modification to be acetamido, corroborating the earlier sole report on this aspect. Based on the biochemical reactions that acetyl coenzyme A is known to participate in, it is difficult to explain the involvement of acetyl coenzyme A in acetamido addition. Notwithstanding the converging predictions of different bioinformatics tools, caution is recommended when inferring function from structurally similar family members. It is possible that a different chemistry might have converged on the same (acetyltransferase) fold, given that none of the known pathways utilizing acetyl coenzyme A can explain the Mom modification. Several likely candidates such as carboxy-SAM, glyoxylic acid and glycine were also tested for being donors of the two carbon entity transferred on adenine by Mom. Since these candidates tested negative in our genetic assays, a genome-wide genetic screen was subsequently devised to identify the host genes involved in mom modification. The assay exploited the phenotype of lethality associated with overexpression of Mom in E. coli in order to screen for mutations in the host genome that rescued the toxicity. However, the survivors which were obtained in this assay had emerged through mutations in the mom gene rather than abrogation of the co-factor synthesis pathway of the host. The results point at two possibilities: (i) utilization of essential gene(s) or (ii) existence of redundant pathways for the Mom modification reaction. Chapter 5 is an account of our attempts to trace the lineage of mom and its regulatory region, employing updated DNA and protein sequence databases. Despite the selective advantage conferred on the phage by the anti-restriction function of mom, in many Mu-like phages, mom is either absent or substituted with methyltransferases. However, in Mu-like genomes that do encode mom, in spite of a significant overall sequence divergence from Mu, the core elements of the mom regulatory circuit seem to have either co-evolved or have been selectively conserved. Although Mu appears to be unique in the possession of a regulatory circuit tailored for the purpose of mom regulation, recently discovered Mu-like genomes show that different types of regulatory features evolved several times in closely related genomes. It is very likely that a toxic gene like mom has earned its place in the phage genome by carrying along with itself a baggage of regulatory elements. Failure to sustain sufficient regulatory pressure may trigger the loss or replacement of the advantageous but potentially lethal mom function.

Climate change is today an urgent threat, posing grave danger and requiring immediate attention. In order to adapt to the changes, policies, plans, and programmes have been created all over the world, such as the National Adaptation Plans (NAPs). Several authors and organisations have advocated for the importance of integrating a gender perspective into such climate change documents (Skinner, 2011; Bee, Biermann, and Tschakert, 2013; Lau et al., 2021). Nevertheless, at the time of writing, research is missing on NAPs in regard to gender. Therefore, the purpose of this thesis is to fill this gap through an analysis of the NAPs with a focus on gender. The sample is 12 countries, all from Sub-Saharan Africa because of the area’s high relevance regarding climate change. The objective of the thesis is to analyse the content of the selected NAPs regarding the integration of gender considerations. Using the theoretical framework of Gender and Development (GAD), the thesis aims to offer a comprehensive understanding of how the chosen NAPs address gender-related concerns. The methodology is a qualitative abductive desk study through qualitative content analysis. The findings suggest that gender considerations are not very well integrated into the NAPs and that women are primarily framed as vulnerable and very rarely as having key capacities or being agents of change. Additionally, from the perspective of GAD, several shortcomings are identified.

No description available.

National Adaptation Plans (NAPs) are central instruments supporting developing countries in their adaptation efforts and enhancing climate resilience. As the NAP documents outline a country's vulnerabilities to climate change and planned adaptation strategies, it is essential to examine how the most vulnerable groups are considered. In the IPCC’s Sixth Synthesis Report, the concepts of maladaptation and transformational adaptation feature prominently, with prior research connecting these concepts to the inclusion of vulnerable groups in adaptation efforts.This thesis provides an in-depth assessment of how vulnerable groups and communities are considered in the three selected NAPs of Bhutan, the Marshall Islands, and Zambia. A normative theoretical framework, the maladaptation-transformative adaptation continuum, is developed from relevant academic research to examine the consideration of vulnerable groups and communities throughout the NAP. Utilising a deductive qualitative content analysis, the central elements of this framework will be operationalised to review the three selected NAP documents. The findings indicate that all of the examined NAPs take vulnerable groups and communities into account by explicitly identifying the most vulnerable social groups, and including adaptation actions that target these vulnerable groups. The inclusion of vulnerable groups and communities in the formulation process and the planned implementation of adaptation measures vary, reflecting different positions along the maladaptation-transformative adaptation continuum.

Made available in DSpace on 2016-04-28T20:57:00Z (GMT). No. of bitstreams: 1 MARIA GORETI MIGUEL SANTOS.pdf: 259181 bytes, checksum: 5043925d5a1c4d1d5326f59b2e1c87b0 (MD5) Previous issue date: 2006-06-02 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Sleep is an activity that has remained unchanged in day care centers which deal with infants up to the age of 3 years old. This paper aimed at investigating how educators of public schools for children dealt with the different sleep and/or vigil rhythms of these children; nevertheless, an analysis of the collected data showed us that there were other practices that have also remained unchanged and that are part of the institutional routine of these children. It is, therefore, of vital importance to re-examine the educational background of those professionals who are responsible for young children. In fact, the data collected shows that there is a waste of time that could be used in a more productive way in terms of how these children develop and learn. As time is not used adequately, there is no progress and the objectives of Children Education tend to be minimized. We hope results shown here will make it possible to debate in depth such an important issue which has not been properly studied yet / O sono é uma atividade de caráter cristalizado nas instituições de Educação Infantil de atendimento à criança de 0 à 3 anos. Este trabalho teve por objetivo investigar como os educadores de uma escola pública de Educação Infantil lidavam com os diferentes ritmos de sono e vigília das crianças de 0 à 3 anos. No entanto, a análise dos dados coletados permitiu identificar, além das respostas buscadas, várias outras práticas cristalizadas, que engendram a rotina institucional das crianças. É urgente e necessário repensar a formação profissional daqueles que atuam junto à criança pequena. De fato, os dados revelam desperdício de um tempo precioso, que poderia ser utilizado em prol do desenvolvimento e da aprendizagem da clientela. Como isso não ocorre, os propósitos da instituição são desvirtuados e o papel da Educação Infantil tende a ser desvalorizado. Espera-se que os resultados aqui alcançados permitam aprofundar e discutir um assunto importante, ainda não devidamente estudado