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Denitrification is the process by which bacteria reduce nitrate to dinitrogen gas. Most denitrifying bacteria perform these reactions under anaerobic conditions only. Thiosphaera pantotropha is one of a number of species capable of aerobic denitrification. During aerobic growth T. pantotropha expresses a periplasmic nitrate reductase but under anaerobic growth conditions nitrate is reduced by a membrane-bound nitrate reductase. The periplasmic nitrate reductase is relatively insensitive to azide and does not reduce chlorate. Aerobic denitrification provides a mechanism to dispose of excess reducing equivalents during growth on reduced carbon sources. Numbers of nitrate reducing bacteria, and nitrate and ammonia concentrations were monitored in a Norfolk broad over a 12 month period. Several novel microorganisms capable of aerobic nitrate respiration were isolated from the sediment of this broad. All were shown to express a periplasmic nitrate reductase activity, and the effects of growth rate and carbon substrate on the activity of this enzyme were studied. Of the nine isolates studied, five were shown to be able to reduce nitrate at oxygen concentrations up to 80% of air saturation. The remaining four were shown to be able to reduce nitrate under anaerobic conditions. Analysis of 16S rDNA sequences was used to identify the isolates, seven were assigned to the Genus Aeromonas, and two to the Genus Pseudomonas. Sediment samples were used to establish a microcosm in which changes in the concentration of nitrate, nitrite and ammoniacal nitrogen were monitored.

Thesis (M.S.)--California Polytechnic State University, 2009. / Mode of access: Internet. Title from PDF title page; viewed on May 22, 2009. Major professor: Nirupam Pal, Ph.D. "Presented to the faculty of California Polytechnic State University, San Luis Obispo." "In partial fulfillment of the requirements for the degree [of] Master of Science in Civil and Environmental Engineering." "March 2009." Includes bibliographical references (p. 88-90). Also available on microfiche.

The purpose of this thesis was to compare ethinylestradiol (EE2) and nitrogen removal in a conventional membrane bioreactor (C-MBR) and a simultaneous nitrification-denitrification membrane bioreactor (SND-MBR). Two MBRs were operated in parallel for 450 days; various MBR operating parameters, total nitrogen removal, and estrogenic activity removal (EA) were measured. The SND-MBR was able to remove 59% of influent TN with an additional 21% removed via sludge wasting; the C-MBR had a TN removal efficiency of only 31%. The C-MBR and SND-MBR removed 57% and 58% of influent EA, respectively. Biodegradation was the dominant removal mechanism for both reactors with KBIO coefficients of 1.5 ± 0.6 and 1.6 ± 0.4 days-1 for the C-MBR and the SND-MBR, respectively. Adsorption removed approximately 1% of influent EA in each reactor. This indicates that SND was able remove greater amounts of TN with no observable impact on EA reduction and membrane operations.

The purpose of this thesis was to compare ethinylestradiol (EE2) and nitrogen removal in a conventional membrane bioreactor (C-MBR) and a simultaneous nitrification-denitrification membrane bioreactor (SND-MBR). Two MBRs were operated in parallel for 450 days; various MBR operating parameters, total nitrogen removal, and estrogenic activity removal (EA) were measured. The SND-MBR was able to remove 59% of influent TN with an additional 21% removed via sludge wasting; the C-MBR had a TN removal efficiency of only 31%. The C-MBR and SND-MBR removed 57% and 58% of influent EA, respectively. Biodegradation was the dominant removal mechanism for both reactors with KBIO coefficients of 1.5 ± 0.6 and 1.6 ± 0.4 days-1 for the C-MBR and the SND-MBR, respectively. Adsorption removed approximately 1% of influent EA in each reactor. This indicates that SND was able remove greater amounts of TN with no observable impact on EA reduction and membrane operations.

Diploma thesis Effect of emergent micropolutants on the denitrification process through activated sludge is focused on three specific substances. The substances under investigation are atrazine, terbutylazine and sulfametaxazole. Atrazine and terbutylazine are pesticides, particularly herbicides. Sulfametaxazole was chosen as a common antibiotic. The thesis is summarized into 10 chapters and conclusion. The work consists essentially of theoretical and practical part. Part of the theoretical part of the thesis is legislation that deals with the matters of the problem with micropolutants. The next chapter is an introduction to the issue, where we want to familiarize the reader with the current situation. There are also chapters on emergent micropolutants, denitrification and investigated substances. If we look at the practical part, there are chapters on sampling, evaluation of BATCH tests and evaluation of inhibition tests. There are also chapters on BATCH tests and respiratory inhibition test, which describe procedures of how we performed this test. Laboratory test reports are included in the Annex.

Thesis (Ph. D.)--Ohio State University, 2007. / Title from first page of PDF file. Includes bibliographical references (p. 115-119).

Low dissolved oxygen (DO) biological nutrient removal (BNR) is becoming a viable option to improve the energy efficiency of BNR. To properly model and design BNR processes for low DO operation, it is critical to fully understand how nitrifier, denitrifier, and polyphosphate accumulating organism (PAO) oxygen kinetics adapt in a shift from traditional DO operation (2 mg O2/L or more) to low DO operation. Research characterizing how oxygen kinetics shift over time in activated sludge biomass adapting to low DO is limited. Therefore, a method to characterize oxygen kinetics for nitrifiers, denitrifiers, and PAOs simultaneously is lacking. Here a method was developed to simultaneously measure the oxygen kinetics of nitrifiers, denitrifiers, and PAOs. This method, termed the SND and P-Uptake Oxygen Kinetics test, was able to estimate the ammonia oxidizing bacteria (AOB) oxygen half-saturation coefficient, ammonia maximum removal rate, denitrifier oxygen inhibition coefficient, total inorganic nitrogen (TIN) maximum removal rate, PAO oxygen half-saturation coefficient, phosphorus maximum uptake rate, and a simultaneous nitrification and denitrification (SND) optimum operation point. Three tests were conducted on the Virginia Initiative Plant (VIP) BNR Activated Sludge Pilot while it was operating at a process DO of 2 mg O2/L, and one test while it was operating at 1.5 mg O2/L. The measurements among the three initial tests showed high similarity in their parameter estimates. Estimated oxygen half-saturation and oxygen inhibition coefficients were compared to current suggested ranges and were within the expected magnitudes. At 2 mg O2/L, denitrifier oxygen inhibition coefficients and PAO oxygen half-saturation coefficients were estimated to be remarkably low here, under 0.4 and 0.1 mg O2/L, respectively. AOB oxygen half-saturation coefficients were variable here in the range of 0.62 to 2.57 mg O2/L, seeming to vary with available ammonia concentrations. Upon comparison with a previously developed respirometric test for nitrifier oxygen kinetics, termed the Declining DO test, the AOB oxygen half-saturation coefficient from the SND and P-Uptake Oxygen Kinetics test and the Declining DO test, when both were conducted on the VIP BNR Pilot, showed a similar trend. This provided validation for the AOB oxygen kinetics here and the usefulness of the test developed here. Additionally, measuring and plotting AOB and denitrifier oxygen kinetics together produced an intersection point where ammonia removal rates were equal to TIN removal rates. This intersection point was an optimum point for SND during the conditions of the test. This method can be used to characterize and track oxygen kinetic changes in a BNR system adapting from high to low DO. / Master of Science / Aerating biological processes in wastewater treatment plants is necessary to facilitate nitrogen and phosphorus removal but is extremely costly. Traditional dissolved oxygen concentrations in these processes are 2 mg O2/L or higher. Operating processes with low dissolved oxygen (DO) concentrations, less than 1 mg O2/L, can cut costs significantly. However, designing processes at low DO concentrations requires knowledge of how microorganisms utilize substrate with lower oxygen availability and how substrate utilization develops when gradually decreasing the DO concentration in a process. Here, a method was developed to measure the parameters describing the relationship between substrate utilization and DO concentration for the microorganisms responsible for nitrogen removal (nitrifiers and denitrifiers) and phosphorous removal (polyphosphate accumulating organisms). Additionally, the method provides an optimum DO setpoint for simultaneous nitrification and denitrification (SND) during testing conditions. This method, termed the SND and P-Uptake Oxygen Kinetics test, was able to estimate the following parameters simultaneously: ammonia oxidizing bacteria (AOB) oxygen half-saturation coefficient, ammonia maximum removal rate, denitrifier oxygen inhibition coefficient, total inorganic nitrogen (TIN) maximum removal rate, PAO oxygen half-saturation coefficient, and phosphorus maximum removal rate. Three tests were conducted on the Virginia Initiative Plant (VIP) BNR Activated Sludge Pilot while it was operating at a process DO of 2 mg O2/L, and one test while it was operating at 1.5 mg O2/L. The measurements among the three initial tests showed high similarity in their parameter estimates. Estimated oxygen half-saturation and oxygen inhibitions coefficients were compared to current suggested ranges and were within the expected magnitudes. Upon comparison with a previously developed test for nitrifier oxygen kinetics, termed the Declining DO test, the AOB oxygen half-saturation coefficient from the SND and P-Uptake Oxygen Kinetics test and the Declining DO test when both were conducted on the VIP BNR Pilot showed a similar trend, providing validation for the usefulness of the test developed here.

Advanced wastewater treatment plants must meet permit requirements for organics, solids, nutrients and indicator bacteria, while striving to do so in a cost effective manner. This requires meeting day-to-day fluctuations in climate, influent flows and pollutant loads as well as equipment availability with appropriate and effective process control measures. A study was carried out to assess performance and process control strategies at the Falkenburg Road Advanced Wastewater Treatment Plant in Hillsborough County, Florida. Three main areas for control of the wastewater treatment process are aeration, return and waste sludge flows, and addition of chemicals. The Falkenburg AWWTP uses oxidation ditches where both nitrification and denitrification take place simultaneously in a low dissolved oxygen, extended aeration environment. Anaerobic selectors before the oxidation ditches help control the growth of filamentous organisms and may also initiate biological phosphorus removal. The addition of aluminum sulfate for chemical phosphorus removal ensures phosphorus permit limits are met. Wasting is conducted by maintaining a desired mixed liquor suspended solids (MLSS) concentration in the oxidation ditches. For this study, activated sludge modeling was used to construct and calibrate a model of the plant. This required historical data to be collected and compiled, and supplemental sampling to be carried out. Kinetic parameters were adjusted in the model to achieve simultaneous nitrification-denitrification. A sensitivity analysis found maximum specific growth rates of nitrifying organisms and several half saturation constants to be influential to the model. Simulations were run with the calibrated model to observe relationships between sludge age, MLSS concentrations, influent loading, and effluent nitrogen concentrations. Although the case-study treatment plant is meeting discharge permit limits, there are several recommendations for improving operation performance and efficiency. Controlling wasting based on a target MLSS concentration causes wide swings in the sludge age of the system. Mixed liquor suspended solids concentration is a response variable to changes in sludge age and influent substrate. Chemical addition for phosphorus removal should also be optimized for cost savings. Finally, automation of aeration control using online analyzers will tighten control and reduce energy usage.

Diss. (sammanfattning). Umeå : Sveriges lantbruksuniv., 2003. / Härtill 5 uppsatser.