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Development of a Biosensor to Detect Landfill Odors Using Human Odorant Binding ProteinUnknown Date (has links)
Landfills receive odor complaints from local residents potentially resulting in
legal ramifications and decreasing quality of life of the neighboring communities.
However, analytical technologies to objectively measure odors in the field that will
reflect human odor perceptions at a reasonable price do not currently exist. Hydrogen
sulfide (H2S) is detectable by humans at low odor thresholds and may be one of the main
nuisance odors emanating from landfills. For the first time, hOBPIIa complexed with 1-
AMA has been used to detect H2S concentrations by spectrofluorometry. The optimal
ratio of hOPBIIa-to-1-AMA was determined to be approximately 1:1. A strong linear
regression model was generated to predict H2S concentrations from peak fluorescent
emission intensity measurements within a range of 0-8.3 g of H2S. A novel
experimental exposure chamber prototype was developed that has the potential to be
incorporated into a portable sensor because it is compact. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2017. / FAU Electronic Theses and Dissertations Collection
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