Diploma thesis was drawn up at Mendel University in Brno, Faculty of Horticulture in Lednice, the Institute of Post-Harvest Technology of Horticultural Products in 2014 - 2016. The purpose of this work was to observe and analyse the influence of selected fining agents on protein stability changes, the concentration of the certain important substances in wine such as polyphenols and anthocyanins before and after their application. Furthermore, organoleptically evaluated clarity and colour changes before and after their wine clarification. During fining process some important substances for the wine are eliminated. Within the analysis of complex polyphenols and anthocyanins after fining process of white, red and rosé wines, the statisticaly significant losses of these substances were recorded in the range of 13 - 33 % of their original value. After comparison the ability of fining agents for white wine can be recommended bentonite Majorbenton B and mixed product Vinosil Plus, for red wines albumin Albuvin and for rosé wines albumin Albuvin.

Cement bonded particleboards contain organic base and as they are set outside, extreme weather condition, such as frost, chemical defrosting substances or sulphates, cause their degradation. The presence of the organic base in the matrix of the material could be the reason why there is higher probability of degrading when compared with materials containing an inorganic base. It could be partially eliminated by an appropriate surface modification of the slab, which main goal is to weaken the impact of an aggressive environment and to extend the lifespan of the board. On the other hand, the surface modifications based on a polymer base are prone to degrade because of other unfavourable conditions such as UV radiation when compared with the cement bonded particleboard. The classification of the surface modifications and the way of their degradation caused by the aggressive environment in real applications are presented in the theoretical part of the thesis. In the experimental part, appropriate methodical ways of testing the resistance of cement bonded particleboard’s coating system were chosen. Then, the testing and comparing of the physical, mechanical and aesthetic properties of different types of coatings were done. Moreover, a spectrophotometric analysis of the colour shade changes for the different aggressive environment were carried out. Furthermore, microstructures of chosen surface modifications were assessed by using an optical and an electron microscope.

As all materials used for exterior construction, cement-bonded particleboards also subject to climatic effects, such as frost, chemicals (eg. sulphates) or UV rays. Degradation of theese effects is slightly increased by organic base. Increased susceptibility can be eliminated by a effective coating, which reduces direct contact with aggresive influences on the board and provides longer durability. Theoratical part of this thesis analyzes different types of negatice influences and their effect on durability. Practical part contains selection of specific types of coating and assessment of their mechanical and aesthetic properties including spectrophotometric analysis of color change for each degradation effect.