No description available.

This study aimed to investigate the feasibility of using several genetic techniques for identification of sulphate-reducing bacteria. A group of eight type strains (seven of which were <I>Desolfovibrio</I>) were used as test group for every method except RSGP, for which an established set of oil field isolates was used. Ribotyping involves restriction fragment length polymorphism (RFLP) analysis of ribosomal RNA (rRNA) operons. Hybridisation of <I>Eco </I>RI-digested genomic DNA to a PCR-amplified (500 bp) rRNA gene product resulted in generation of discriminatory RFLP patterns for all but two of the eight test strains. Restriction digestion of PCR-amplified (1400 bp) rRNA gene products (PCR-robotyping) allowed differentiation between all eight strains for two of the seven enzymes tested. Random amplified polymorphic DNA (RAPD) analysis allows PCR-generation of distinct sets ("fingerprints") of amplification products from genomic DNA templates. Nine oligonucleotide decamers were tested as primers, two of which were found to generate discriminatory profiles for each of the eight type strains. Hybridisation of 32P-labelled RAPD products against genomic DNA from eleven sulphate-reducing strains was found to result in specific hybridisation of probes to their complementary genomic DNA. Reversal of the hybridisation procedure, using 32P-labelled genomic DNA against immobilised RAPD probes, was found to allow analysis of mixed genomic DNA samples in a single step. This reverse method is very similar in principle to RSGP, which allows analysis of mixed genomic DNA preparations by hybridisation against a master filter of genomic DNAs from a series of environmental bacterial standards. This study developed a method to allow quantitation of RSGP hybridisation signals, and went on to analyse biofilm samples from various locations in Western Canadian oil fields, resulting in identification of 21% (average) of each mixed DNA sample.

El presente trabajo tiene por finalidad dar a conocer las actividades realizadas en la Cia. MAPLE GAS CORPORATION DEL PERU SRL, empresa en la cual ingrese a laboral el 12 de Mayo del 2003 hasta el 5 de setiembre de 2009.

Thesis (M.S.)--Ohio State University. / Bibliography: leaves 62-64. Available online via OhioLINK's ETD Center

Thesis (M.S.)--Ohio State University, 1967. / Available online via OhioLINK's ETD Center

Thesis (Ph. D.)--University of Wisconsin--Madison, 1982. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph.D.)--University of Wyoming, 2009. / Title from PDF title page (viewed on June 10, 2010). Includes bibliographical references.

No description available.

Thesis (Ph. D.)--University of Texas at Austin, 2003. / Vita. Includes bibliographical references. Available also from UMI Company.

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