The goal of the diploma thesis is to put forward a procedure for implementation of an institutional repository at the Czech University of Life Sciences in Prague with the aid of an analysis on the current situation of institutional repositories in Czechia. The analysed repositories are selected using OpenDOAR and ROAR registries. The first part of the thesis describes the software used to implement the institutional repositories in Czechia. In the next part tools, standards and certifications relevant for trustworthy institutional repositories such as DRAMBORA and PLATTER, standards ISO 16363 and ISO 14721 and the so called lesser certifications Data Seal of Approval and Nestor Seal of Trustworthy Digital Archives are described. Following part focuses on policies and operation of those analysed repositories. The last part of the thesis deals with a description of current practice on the Czech University of Life Sciences, the conducted quantitative analysis among the researchers of the university and the aforementioned procedure for implementation of an institutional repository using the tool PLATTER. The conclusion summarizes the issues of institutional repositories in Czechia.