Thesis (M. Phil.)--University of Hong Kong, 1987.

Thesis (M.S.)--Villanova University, 2007. / Chemistry Dept. Financial support provided by the National Science Foundation. Includes bibliographical references.

Thesis (Ph.D.). / Written for the Dept. of Bioresource Engineering. Title from title page of PDF (viewed 2008/07/24). Includes bibliographical references.

Composting is a sustainable method to deal with huge amount of daily organic waste due to its robustness and easy operation. However, food waste (FW) as the main material in composting has disadvantages such as the heterogenous properties, high foreign matters contamination, high moisture content, low C/N ratio, poor structure, low porosity and high acidity during the initial phase of composting. These shortcomings not only influence degradation efficiency but also cease the composting process. Therefore, a bulking agent is required to increase the porosity and adjust the moisture content as well as C/N ratio of the composting mixture (Wong et al., 2010). For previous research, sawdust (SD) and tree barks were commonly used as the bulking agent in composting system but the demand for sawdust and tree barks significantly increased the cost of the composting process, and this has stimulated the demand of alternative substitutes. Therefore, the ideal situation is to find the bulking agent which is not only suitable for composting but is also a waste. Traditional Chinese medicine is widely used nowadays and huge amount of residues are accumulated and treated in landfilling (Wang and Li, 2013). According to previous research, only 5% of the active ingredients can be extracted from the medicinal plants which means there are still a large fraction of active ingredients remain in the herbal residues (Wu et al., 2013). In addition to the bulking property of Chinese medicinal herbal residues (CMHRs), it is assumed mature CMHRs compost have the ability to hinder regular metabolic pathway of phytopathogens after land application (Bernal-Vicente et al., 2008). The first experiment of this study investigated the formula between food waste, sawdust and CMHRs in order to achieve efficient composting. The experimental results demonstrated positively the use of CHMRs is a suitable candidate to co-compost with food waste. In terms of biodegradation decomposition efficiency and compost maturity, the treatment 1:1:1 (FW: SD: CHMRs, dry wt. basis) showed the best performance among all treatments with 67% organic matter degradation and 157% seed germination index. Only well-matured composting product can suppress plant diseases in soil since it has some microorganisms which can inhibit phytopathogens. The treatment 5:5:1 (FW: SD: CHMRs, dry wt. basis) also reached maturity but with a longer composting period; however, it was the treatment which could accommodate the highest quantity of food waste. The log copy number of the bacterial population was 7-8 initially, which decreased and stabilized along the composting. Results revealed that the CHMRs can be used as a bulking agent with food waste, and a dry weight ratio of 1:1:1 (FW: SD: CHMRs) would be optimum to achieve higher organic decomposition and faster maturity. However, the initial lower microbial population in the treatment, though without any adverse effect on the overall microbial decomposition, will warrant further work to indicate the total population is not a practical means to illuminate the effective microbial decomposition. Besides, the advantage in using CHMRs will need further experiment to indicate its potential pathogen suppression capability. Humification during co-composting of food waste, sawdust and CMHRs was investigated to reveal its correlation with compost maturity. The huge decrease in the treatment 1:1:1 (FW: SD: CHMRs, dry wt. basis) of aliphatic organics in humic acids (HA) demonstrated the degradation of the readily available organics, while an increase in aromatic functional groups indicated the maturity of compost. Disappearance of hemicellulose and weak intensity of lignin in the CMHRs treatments indicated that the lignin provided the nucleus for HA formation; and the CMHRs accelerated the compost maturity. Humic acid to fulvic acid (HA/FA) ratio of 1:1:1 treatment was the highest at the end of composting and showed a clear correlation with compost maturity as also evidenced through the presence of higher aromatic functional groups in the HA fraction. Pyr-TMAH-GC-MS results indicated that dominant groups were aliphatic and alicyclic esters and ethers at the early composting stages in all treatments. Long chain fatty acids were broken down into smaller molecular compounds earlier in treatment 1:1:1 (FW: SD: CHMRs, dry wt. basis), resulting from the faster decomposition rate. The complicated ring-structure components appeared dominantly at the later phase of composting. The peak intensities in treatment 1:1:1 (FW: SD: CHMRs, dry wt. basis) indicated that the composts became mature earlier than the other two treatments. In brief, the treatment with dry weight ratio 1:1:1 had greatest humification degree with more cyclic structures and stable final products at the end of composting. Water and acetone extract of composts with food waste and CMHRs were tested with their antipathogenic effect on two kinds of commonly found phytopathogens, Alternaria solani (A. solani) and Fusarium oxysporum (F. oxysporum). Seventeen bacterial species and 22 fungal species were isolated and identified as prevalently existed microbes during composting process. The results of MIC50 indicated that the treatment with dry weight ratio 1:1:1 (FW: SD: CHMRs, dry wt. basis) required least concentration of composts extraction to kill half quantity of the phytopathogens, 16% for A. solani and 22% for F. oxysporum extracted by acetone. The phytopathogen suppression capacity of composts was partially due to antagonistic abilities from some of the isolated microorganisms as well as the inhibition of active compounds. As shown in the comparison, the interfere/compete between antagonistic microorganisms and target pathogens were more powerful than individually influenced by chemical compounds. However, the influencing factors should not be considered independently since antagonistic interactions between microbes in composts and phytopathogens are highly dependent on the abiotic properties of the composts and the alternative environment. In a word, the antipathogenic effects from composts were synergism of both antagonism and chemical factors. Suppressive capacity on phytopathogens is one of the major function of mature composts and the antipathogenic effect was stimulated when CMHRs was used as the bulking agent in composting process. The abiotic inhibitory rates of treatment 1:1:1 (FW: SD: CHMRs, dry wt. basis) indicated that more powerful bioactive components were remained at the end of composting than in the treatment 5:5:1 and control which had no CMHRs but plastic beads as the bulking agent. Hence sensitive and comprehensive analytical technique of ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-QTOF-MS) was utilized to acquire a better understanding of the complicated structures of final composting products. Seven dominant among 22 active compounds with antibacterial/antifungal properties were obtained in the treatments with CMHRs while 17 kinds of compounds with higher contents were shared in all treatments, which should be derived from food waste. The bioactive components from CMHRs composting were mainly from the groups of alkaloids, flavonoids and coumarins. Mature composts were used as biofertilizer to protect plants (Brassica chinensis and Lycopersicon esculentum) from phytopathogenic infection. This study showed the crop yields were increased with the addition of mature CMHRs composts to acid soil, and 5% CMHRs compost was the optimum application rate, while at the higher application rate of 10% (dry weight basis, w/w) plant growth was inhibited which might be due to the higher salt contents and the phytotoxicity of alkaloids, flavonoids and coumarins in the CMHRs. According to the biomass results, Brassica chinensis was more sensitive to the inhibitory effect of phytopathogen inoculation, while nutrient supply was to a less extent due to the short growth period as compared to Lycopersicon esculentum. The present study showed clearly that mature compost provided Lycopersicon esculentum and Brassica chinensis sufficient nutrients such as nitrogen and phosphorus. Additionally, the advantage of using mature CMHRs compost as a soil conditioner was also observed for blocking phytopathogenic infection from plant roots. The mechanism was mainly derived from the bioactive components in mature CMHRs compost which inhibited phytopathogenic activities in soil. Many identified compounds were alkaloids, flavonoids and coumarins which have powerful antifungal and antibacterial abilities and most of them maintained during growth period though their amounts reduced greatly due to their photolytic and pyrolytic properties. Therefore, mature CMHRs compost can be the substitute to reduce the usage of fungicides and its associated environmental hazards. The present study demonstrates clearly the beneficial effects of using CMHRs as a bulking agent to co-compost with food waste with the additional phytopathogens suppression property. Therefore, it is concluded that Chinese medicinal herbal residues can be a good choice of bulking agent in food waste composting system. Organic matter degradation and humification process were accelerated by CMHRs addition and mature CMHRs compost had antipathogenic effect and protect plants from infection

Composting is considered as an effective and sustainable food waste treatment technology from the perspectives of volume reduction, stabilization and releasing the pressure on landfills. Community composter is a decentralized composting facility in fed-batch operational mode which is usually being installed in the backyard of institutes, hospitals, housing estate etc. to handle the food wastes generated daily. Albeit numerous operational issues including high initial acidity and oil content, poor decomposition and odor generation are commonly encountered in these facilities, which make it difficult to be accepted by the public. Therefore, the aim of the present study is to develop a composting mix formulation that can provide a solution to all these issues in a fed-batch food waste composting process. The first phase of this study aims at finding out an optimized formulation in a batch-scale food waste composting process through the use of alkaline amendments and microbial inoculum. For the first two experiments, artificial food wastes were prepared by mixing 1.3kg bread, 1kg boiled rice, 1kg cabbage, 0.5kg fully boiled pork and mixed with sawdust to obtain a C/N of 30 and adjusted moisture of the mixtures to 55%. The effect of different concentrations of zeolite compared to lime was studied in the first experiment. Zeolite was amended with food wastes and sawdust mixtures at 2% (ZI-2), 5% (ZI-5), 10% (ZI-10) to compare with lime in 2.25% (L-2.25) w/w (dry weight basis) and composted for 56 days. Results demonstrated that 10% of zeolite was optimal amendment rate compared to lower dosage of zeolite (2% & 5%) with stronger pH buffering capacity and greater decomposition efficiency. Addition of 2.25% of lime buffered the pH efficiently but increased the ammonia loss significantly which eventually reduced total nitrogen (TN) content of final product and posed odor emission problem. Amendment of 10% zeolite provided a higher adsorption affinity on ammonia resulting in 2.05% of TN value of final product which was higher than 1.72% of lime treatment. Furthermore, significantly higher seed germination 150% was achieved of ZI-10 compost compared to 135% of L-2.25 due to low ammonium content of product. The first experiment showed that application of less than 10% zeolite was not sufficient to buffer the acidity; as a result, organic matter decomposition was inhibited. However, the cost and reduction in treatment percentage of food waste in 10% application rate of zeolite is an issue of concern. To tackle this dilemma, food waste was amended with struvite salts at 1:2 molar ratio of MgO and K2HPO4 (Mg:P) with or without zeolite amended at either 5% or 10% amendment (Mg:P, Z5 + Mg:P & Z10 + Mg:P) and a control treatment with food waste only was also included. Results showed that treatment of Z10 + Mg:P was synergistically achieved of pH and EC buffering, and N conservation but not for the case of 5 % zeolite. Treatment of Z10 + Mg:P further reduced the N loss to 18% compared to 25% and 27% of Mg:P and Z5 + Mg:P respectively. However, there was insignificant difference in the final nitrogen content and decomposition rate among all treatments with struvite salts amendment. Comparing to the treatment of Z-10 of the first experiment to Z10 + Mg:P of the second experiment, Z-10 showed superior performance since better decomposition efficiency, shorter time to require to pass the GI (28 Days) and lower cost because of salts exclusion. To develop a multipurpose formulation for the fed-batch operational food waste composter, high lipids problem in food waste cannot be neglected because it is a critical factor to hinder the decomposition efficiency. Inoculation of oil degradative microorganisms was reported as an effective approach to facilitate the lipids. Therefore, the third experiment was to investigate the overall composting performance supplemented with 10% zeolite and microbial consortium. 10% zeolite with bacterial consortium significantly reduced the lipid contents from 7% to 1% compared to control treatments. Furthermore, treatments amended with 10% zeolite was proved to reduce ammonia emission and total volatile fatty acids level in the composting mass, therefore the total odor emission level can be reduced. Zeolite at 10% was found to be a suitable optimum additive for both synthetic and real-food wastes. Therefore, treatment of 10% zeolite with bacterial consortium is selected as an optimized formulation for further study of its application in a fed-batch composter. Following the food waste zeolite composting formulation obtained in Phase I, the aim of Phase II was to develop an ideal composting mix formulation for on-site commercial composters. Although the results have been demonstrated 10% zeolite with bacterial consortium facilitated the composting efficiency in batch composter, those amendments may be over-estimated if applied in a fed batch composter by using real food wastes. With this constraint, the applicability of these additives in commercial fed-batch composter needs to be assessed using locally generated food wastes. Treatments included food waste and sawdust mixtures at 4:1 mixing ratio (wet weight basis) were mixed with 2.25% of lime (L2.25), 10% of zeolite (Z10) and 10% zeolite with bacterial inoculum (Z10+O) and a control of food waste with sawdust mixture only was also included. 35 kg compost mixture was fed into each composter respectively daily for a period of 42 days. Only Z10+O was the most suitable composting mix for fed-batch food waste composting process with continuous sustained high temperature (55-60oC), optimal moisture (55%-60%), alkaline pH and low EC during the experimental period. Bacterial inoculum significantly improved the lipids decomposition from 22.16% (C) to 3.10% (Z10+O) after the composting period. In contrast, lime and zeolite alone treatments could not maintain the optimal pH that led to reduce degradation and longer stabilization period. Only compost taken from Z10+O treatment could be classified as mature compost. The aim of the third study phase was to examine an optimal application rate of food waste compost produced from decentralized food waste composter for plant. A plant growth experiment was conducted in this phase to evaluate the change in soil properties and plant growth of Brassica chinensis and Lycopersicon esculentum. The experiment was conducted in a loamy soil amended with 0%, 2.5%, 5% and 10% food waste compost amendment rate compared to the control soil with chemical fertilizer amendment only. Results indicated that 5% was the optimal application rate of food waste compost for both crops among all treatments which can be evidenced by the highest biomass production and nutrients value of the plant tissues. Plant available nutrients such as NH4+, NO3-, PO43- were proportionally increased with increase in compost application rate. However, 2.5% of the food waste compost did not provide sufficient nutrients for plant growth and 10% showed negative effects due to increased salts content. Plants amended with chemical fertilizer had relatively low biomass production compared to compost amended treatments due to soil compaction and fast leaching of nutrients. It can be concluded that application of 10% zeolite with microbial consortium is an ideal composting mix formulation for on-site commercial composters and 5% is an optimal application rate of food waste compost of Brassica chinensis and Lycopersicon esculentum

No description available.

Thesis (M.S.)--Cleveland State University, 2009. / Abstract. Title from PDF t.p. (viewed on Feb. 16, 2010). Includes bibliographical references (p. 82-89). Available online via the OhioLINK ETD Center and also available in print.

Thesis (doctoral)--Swedish University of Agricultural Sciences, 2005. / Includes bibliographical references. Also available on the World Wide Web.

The Federal Water Pollution Control Act Amendments of 1972 prohibits the discharge of seafood processing solid wastes into navigable waters after July 1, 1977. Oregon shrimp and crab processors must use other methods of disposal for the 15 to 30 million pounds of solid waste generated annually. The application of shrimp and crab wastes to nearby agricultural land can consume the wastes generated at major processing ports. As they came from the processing plant, shrimp and crab solid wastes contained 1.3% to 1.6% N, 0.47% to 0.54% P, other nutrients, 7% to 14% CaCO��� equivalent, and 64% to 78% water. A greenhouse experiment was established to determine the effects of 1) grinding the wastes, 2) surface vs. incorporated waste applications, and 3) waste applications vs. inorganic N applied at equivalent N rates (56, 168, and 336 kg N/ha) with applications of P, S, and lime supplied with the inorganic N only. The fertilizer materials were applied on two coastal soils, and two pasture crops were grown. Forage yields and the P concentration in 'Potomac' orchard grass (Dactylis glomerata L.) were significantly higher with incorporated waste applications than with surface waste applications. Application method did not affect the P concentration in New Zealand white clover (Trifolium repens L. ). The difference in crop response between application methods would assumably be less under field conditions than was measured in the greenhouse. Grinding crab waste significantly increased forage yields when the waste was surface applied, but not when incorporated with the soil. Unground shrimp waste gave significantly higher forage yields than ground shrimp waste. No significant difference occurred in the forage yields, the N uptake by orchardgrass, or the P concentrations in orchardgrass and white clover among applications of shrimp waste, crab waste, and inorganic nutrients with lime. Applications of shrimp and crab wastes increased white clover yields over the control by a factor of more than 3.5 on Knappa silt loam (pH 4.9 - 5.0) but did not measurably increase the soil pH. It was assumed that the wastes, in the immediate area of the shell material, increased the availability of Ca, P, S, and Mo, decreased soluble soil Al, and allowed effective rhizobial nodulation and N fixation. Increasing application rates of shrimp and crab wastes to Knappa and Nehalem silt loams significantly increased the extractable soil P and Ca, and significantly decreased the extractable soil K after 28 weeks of orchardgrass growth. No consistent effect on soil pH was measured. In a second greenhouse experiment, N rates of 165 and 330 kg/ha and P rates of 61 and 122 kg/ha were supplied by shrimp waste and by inorganic sources to a limed coastal soil in a 2 x 2 x 2 complete factorial arrangement. Applications of shrimp waste resulted in significantly higher orchardgrass yields and P uptake than applications of the inorganic nutrients, but no significant difference occurred in the N uptake. In an irrigated coastal pasture, fresh shrimp waste was applied at 6,726, 17,936, and 35,872 kg/ha and ammonium phosphate (16-20-0 15 S) was applied at 224 and 448 kg/ha and a stand of orchardgrass was established. Forage yields were higher with shrimp waste than with ammonium phosphate. Shrimp waste applications beyond 17,936 kg/ha did not further increase the forage yield or P uptake. Shrimp waste applications increased extractable soil P, SO��� -S, soluble salts, and NO��� -N, but resulted in a depletion of soil K when measured at the end of the growing season. Shrimp and crab processing wastes are effective sources of N and P for crop plants and should be applied at rates necessary to supply the recommended rates of N. / Graduation date: 1978

Thesis (Ph. D.)--Washington State University, December 2009. / Title from PDF title page (viewed on Feb. 19, 2010). "Department of Biological Systems Engineering." Includes bibliographical references.