The aim of this work is to assess the influence of mowing and grazing with different frequency and fertilization on species composition, fodder value and bimas production. The experiment was carried out at the village of Hrazany, district of Písek in 2017. The ecological conditions of grasslands, terms and intensity of use, biomass production, botanical composition and species diversity were monitored on these parcels. The data obtained was used to calculate the Hill's Diversity Index, calculate the pasture load, and calculate the yield in dry matter. Non-fertilized plots reach the lowest yields. Most grasses are located on the soil fertilized with urine. The smallest disturbance and shortcomings appear on the pasture with the finch pasture. The values were statistically processed.