Thesis (Ph.D.) -- University of Adelaide, Dept of Anatomical Sciences, 2003? / Bibliography: leaves 262-298.

Bibliography: leaves 262-298. This thesis investigated the structure and glycoconjugate composition of the zona pellucida (ZP) surrounding marsupial oocytes and the changes that occur during ovarian development, following ovulation, and following cortical granule exocytosis. The glycoconjugates of the oviduct epithelial lining of the brushtail possum around the time of ovulation were also examined to determine if there was any contribution of the oviductal secretions to the post-ovulatory ZP.

Bibliography: leaves 262-298. / 300 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis investigated the structure and glycoconjugate composition of the zona pellucida (ZP) surrounding marsupial oocytes and the changes that occur during ovarian development, following ovulation, and following cortical granule exocytosis. The glycoconjugates of the oviduct epithelial lining of the brushtail possum around the time of ovulation were also examined to determine if there was any contribution of the oviductal secretions to the post-ovulatory ZP. / Thesis (Ph.D.)--University of Adelaide, Dept. of Anatomical Sciences, 2003?

published_or_final_version / Obstetrics and Gynaecology / Doctoral / Doctor of Philosophy

Graduation date: 1993

Immunocontraception, or contraception mediated by the immune system, is being widely studied as an alternative, humane form of population control. The induction of an immune response against a specific component of the mammalian oocyte, termed zona pellucida (ZP) has been shown to be an effective immunocontraceptive in several species. Comparatively little work has been done investigating the use of ZP antigens in the domestic cat. This study aimed to investigate porcine ZP(B+C) and feline ZPA, B and C as immunogens in the domestic cat, and further to elucidate their effects on reproduction. Immunisation of female cats with porcine ZP(B+C) failed to elicit a detectable antibody response as assessed by ELISA, immunoblotting and immunohistochemistry. Additionally, there was no effect on the structure of the ovaries nor on breeding performance. Feline ZPA, B and C were cloned into the pkCMVint.polyli mammalian expression vector and used to immunise female cats. ELISA revealed that immunisation with either pkCMVint.fZP(B+C) or pkCMVint.fZPA DNA (Treated) resulted in a low-level circulating antibody response, which was apparently short-lived. Immunoblotting did not reveal any common pattern of recognition of antigenic polypeptides between responding animals. Ovaries from fZP Treated animals, however, showed antibody binding specifically on the ZP of follicles from late primary / early secondary, through to antral stages. Despite the antibody binding, the overall structure of the ovaries remained unaffected in all but two of the fZP Treated cats. Two fZPA immunised cats exhibited ovaries that had no recognisable follicular structures, however, the observed abnormalities could not be conclusively linked to fZPA immunisation. Overall, a significant lymphoproliferative response was shown in fZP Treated cats when compared with Controls. Fertility was not significantly affected in fZP Treated cats, although there was a tendency towards increased incidence of unsuccessful matings or pregnancies. The fZP DNA preparations studied here exhibit potential as an immunocontraceptive, with the ability to generate a lymphoproliferative response against fZP and elicit antibodies specifically recognizing fZP in situ. Further studies should continue to investigate the immunogenicity of, and characterise the immune response against, such fZP DNA preparations.

Thesis (Ph.D.)--Murdoch University, 2007. / Thesis submitted to the Division of Health Sciences. Bibliography: leaves 207-217.

Thesis (Ph. D.)--University of Virginia, 1998. / Includes bibliographical references (130-147). Also available online through Digital Dissertations.

Thesis (Ph. D.)--University of Hong Kong, 2009. / Includes bibliographical references (leaves 126-155) Also available in print.

University, Diss., 2005--Leipzig.