The Orchids Erythrodes nobilis, Macodes petola, Ludisia discolor, Erythrodes hirsura, Dosinia marmorata a Anoectochilus sp. belongs to a group of terestrial orchids of south-eastern Asia. These orchids are adapted to life the undergrowth of tropical rain forests. This thesis deals with the adaptations to shading of these orchids especially with the anatomical adaptations and the concentration of pigments (chlorophyll a, chlorophyll b, carotenoids, anthocyanins). The typical attributes of the leaf anatomical structure of the species studied include one-layer epidermis consisting of lens-like cells, and the upper layer of mezophyll with signs of palisade parenchyma and high content of chlorophylls.

This thesis is focused on verifying in vitro cultivation techniques in micropropagation of tropical terrestrial orchid Macodes petola (Bl.) Lindl. The aim of the studies was to verify the responsiveness of the node explants of Macodes petola (Bl.) Lindl to different surface-sterilization methods and duration of sterilization time, as well as the transport of the node explants to agar medium. Node explants of Macodes petola (Bl.) Lindl were surface-sterilized with different disinfectants for different sterilization time and cultivated in primordial culture. The best results were observed by using sequential surface sterilization in ethyl alcohol (70%) and disinfectant Savo (2.5%) - ethyl alcohol being used for 15 seconds and Savo for 20 minutes.

The orchid family covers 90 percent of the rarest plants under protection all over the world. The ecological and economic value of orchids has aroused public awareness. Since vegatitve propagation methods are available, development of in vitro methods are essential for conservation and commercialization of plants. Purposes work was elaboration progress of vegetative multiplication for tropical orchid Macodes petola in conditions in vitro. At research were proof 4 methods, which distinguish in using desinfection, in operating time and in using different nutritive mediums.