The aim of the work was to evaluate the milk production and fertility of the herd of Czech piebald cattle and to express the economic impact of the use of various breeding bulls used for breeding. The results of milk yield and insemination were evaluated in the years 2017 - 2018 for the last closed lactation sequence in the groups according to the order of lactation (1st lactation, 2nd lactation, 3rd lactation, 4th and next lactation). The indicators were evaluated for the three most commonly used breeding bulls. The average efficiency by the lactation sequence was in the 1st lactation 5143 kilogrammes of milk (P 0.001), the 2nd lactation 6520 kilogrammes of milk (P 0.01), the 3rd lactation 8186 kilogrammes of milk (P 0.001) and in the 4th and next lactation 7855 kilogrammes of milk. The efficiency of the whole herd was 6856 kilogrammes of milk contains 4.09% of fat and 3.46% of proteins. The avarige age at the first calving was 945 days, the length of the herd´s interval was 416 days, the insemination interval was 93 days and the service period was 129 days. The success rate of the 1st insemination in 2017 was 75%, in 2018 74%. The success rate of all insemination was 69% in both years. The costs of insemination of the whole herd with using all bulls in 2017 was 618 CZK and in 2018 was 577 CZK. Was found economic difference in using individual breeding bulls included in reprodudtion in a given herd.