Thesis (Ph. D.)--University of Missouri-Columbia, 2005. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file viewed on (May 22, 2006) Vita. Includes bibliographical references.

Dissertation submitted in compliance with the requirements of the Master's Degree in Technology in Biotechnology, Technikon Natal, 1999. / Sugarcane mosaic potyvirus (SCMV) IS the causal agent of the most important viral disease of sugarcane in South Africa, mosaic. Accurate knowledge of the prevalent SCMV strain(s) in the South African sugar industry is lacking and has never been determined using molecular analysis. Identification of SCMVstrains at the genomic level would provide valuable information for the development of appropriate in vitro diagnostic tests and in the genetic engineering of sugarcane for coat protein (CP) mediated resistance. / M

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The nematode surface coat is defined as an extracuticular component on the outermost layer of the nematode body wall. Surface coat proteins of Meloidogyne incognita race 3 infective juveniles were characterized by electrophoresis and Western blotting of extracts from radioiodine and biotin-labeled nematodes. Extraction of labeled nematodes with cetyltrimethylammonium bromide yielded a principal protein band with a molecular weight larger than 200 KD and several faint bands of lower molecular weight ranging from 31 KD to 179 KD. Western blots of unlabeled proteins were probed with a panel of biotin-lectin conjugates, but only Concanavalin A bound to the principal band. Nematodes labeled with radioiodine and incubated in water for 20 hours released ¹²⁵I into the water, indicating that surface coat proteins may be loosely attached to the nematode. Antiserum to the principal protein reacted with the surface of live nematodes and with surface proteins previously separated by electrophoresis. Differential patterns of antibody labeling were obtained on Western blots of extracts from M. incognita race 1, 2, and 3, Meloidogyne hapla race 2, and Meloidogyne arenaria race B. One biological role of the surface coat may be to act as the receptor site for attachment of the nematode bacterial parasite, Pasteuria penetrans. This possibility is suggested because antiserum raised against surface coat proteins of Meloidogyne incognita race 3 reduced bacterial attachment.

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