Th. Ph. D.--Social sciences. / Bibliogr. p. 328-354. Notes bibliogr. Index. Glossaire.

Th. 3e cycle--Sci. écon.--Paris 10, 1985.

Proefschrift--Rijksuniversiteit te Leiden, 1994. / La couv. porte comme nom d'auteur : "Ruud Beeldsnijder" Bibliogr. p. [319]-335. Index. Résumé en anglais.

Proefschrift--Amsterdam--Vrije Universiteit, 1991.

Texte remanié de: Proefschrift--Letteren--Rijksuniversiteit te Utrecht, 1989. / Bibliogr. p. 525-537. Index.

Texte remanié de: Doctoral diss.--University of Göteborg, 2002. / Bibliogr. p. 203-226.

Thesis (Ph. D.)--University of Queensland, 2002. / Includes bibliographical references.

Nitrogen (N) derived from symbiotic fixation of atmospheric N2 in wild and cultivated populations of Cyclopia, a woody endemic genus used to make honeybush tea in the Western Cape of South Africa, was quantified by the 15N natural abundance method. Because Cyclopia species are naturally mycorrhizal, non-N2-fixing arbuscular mycorrhizal shrubs of similar phenology to Cyclopia were chosen as reference plants to provide the d15Nvalue of soil-derived N. Isotopic analysis showed that wild populations of Cyclopia were highly dependent on N2 fixation for theirNnutrition, ranging from 70 ± 4%to 100 ± 7% (mean ± SE) at all sites, except for one. Further evidence of the high dependency of wild Cyclopia populations on symbiotic N was provided by their significantly higher foliar N concentrations compared with the non-legume reference plants. However, cultivated Cyclopia exhibited variable amounts of N2 fixation, with Cyclopia genistoides (L.) R. Br., for example, showing low amounts of N2 fixation at Sites P2 and P3 (0 ± 51% and 8 ± 46%, respectively) as a result of low D values (D is defined as the difference between the mean d15N value of the reference plants and the B value of the test Cyclopia species, where B is the d15N of an inoculated test legume grown in an N-free growth medium), whereas at Sites P1, P2, P5 and P6, up to 89 ± 2%, 94 ± 13%, 85 ± 13% and 100 ± 18%, respectively, plant N was derived from atmospheric fixation. The high symbiotic N nutrition observed for wild populations of Cyclopia suggests that these populations are major contributors to the N economy of the nutrientpoor soils of the South African fynbos. These data indicate that by breeding for high N2 fixation rates in Cyclopia cultivars and selecting more efficient rhizobial strains, this legume has the potential to achieve higher N2 fixation rates under cultivation. The low variability in Cyclopia d15N values within sites, however, suggests that genetic variability is not a major factor influencing N2 fixation rates in cultivated Cyclopia, and thatmore benefit may be gained from soil amelioration and the selection of improved rhizobial strains

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