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Amperometric Glucose Biosensor by Means of Electrostatic Layer-by-layer Adsorption onto Electrospun Polyaniline FibersShin, Young J. 2009 May 1900 (has links)
An amperometric glucose biosensor was fabricated using electospun polyaniline
fibers. Polyaniline was reacted with camphorsulfonic acid to produce a salt, which was
then dissolved in chloroform containing polystyrene. Using this solution, fibers were
formed and collected by electrospinning. Glucose oxidase was immobilized onto these
fibers using an electrostatic layer-by-layer adsorption technique. In this method,
poly(diallyldimethylammonium chloride) was used as the counter ion source. The level
of adsorption was examined and evidence of layer-by-layer adsorption was obtained
using a quartz crystal microbalance technique. A biosensor was fabricated from these
fibers as a working electrode, and used to measure the glucose concentration accurately.
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