This thesis is focused on the application and optimization of biotechnological methods within the family Brassicaceae. Most of this thesis is focused specifically the genus Brassica. The main objective of this dissertation thesis was to optimize and apply selected biotechnological methods in the resynthesis of oilseed rape (B. napus) from its baseline species (B. oleracea, CC and B. rapa, AA) and subsequently create valuable genetic plant materials. The optimized procedure implemented in my work have exhibited better results of hybrid production (resynthesis) than in similar published experiments and provides a simplified and less laborious method via simple ovule culture and early hybrid verification. The next accomplished step was to work with a wider range of materials using a broader source of different and contrasting genotypes from Brassica rapa (spring and winter oilseed and vegetable turnips) and B. oleracea (green and purple curly kales and cabbages) for new combinations and wider genetic diversity. It was concluded that the optimized ovule culture protocol with early verification, developed in the framework of this thesis, is satisfactorily sufficient enough to be applicable in breeding programmes, aimed at diversity expansion of winter oilseed rape gene pool, as the resynthesized embryos were derived in most combinations. New resynthesized verified lines were colchicine treated in order to obtain diploid regenerants and the fertile plants were self-pollinized and crossed with elite oilseed rape lines for further research and breeding.

The master thesis deals with the possibility of using dynamic damper in hydrodynamics. Specifically, the assessment of the impact of dynamic damper on the dynamic stability of the fluid system when the system exhibited unstable behaviour prior to damper installation due to self-excited vibration. In the thesis is presented the algorithm for the calculation of pressure and flow pulsations based on the transfer matrix method, the transition matrices for different damper designs variants are derived. Using the algorithm, the effect of a damper placed in a stable and unstable fluid system is solved. The output of this work is software for solution of pressure and flow pulsations in the system with serially arranged hydrodynamic elements created in the MATLAB program.

This thesis deals with the optimization determination of chondroitin sulfate sequential injection analysis with spectrophotometric detection. The reaction proceeds in the presence of phenothiazine cationic dyes (Azure A, Azure B and methylene blue) and measuring the decrease absorbance dyes in their absorption maximum after the addition of chondroitin sulfate. Have found the optimum conditions for this determination: dye concentration 5 10-5 mole dm-3 , the dosing volume 100 µl CS + 50 µl dye + 100 µl CS, reaction time 0 s, flow rate 40 µl s-1 . For the determination of the CS in a static arrangement was found the lowest limit of detection and quantification using metylene blue (LOD = 0,23 mg l-1 a LOQ = 0,76 mg l- 1 ). The best repeatibility was achieved also using methylene blue 2,4 %. On the other side the best sensitivity was achieved using azure A. For the determination of the CS in SIA arrangement was found the lowest limit of detection and quantification using azure (LOD = 0,34 mg l-1 a LOQ =1,01 mg l-1 ). The best repeatibility was achieved also using azure A 1,9 %. And the best sensitivity was achieved using azure B. Based on these findings was chondroitin sulphate determined in food supplements, the more suitable method were the calibration curve .The determination was also carried out...

This diploma thesis deals with project and experimental verification of mathematical model of experimental circuit for measuring turbine in laboratory of Kaplan Department of Fluids Engineering FME BUT in Brno. Pressure and flow characteristics were modeled based on data measured on general-purpose experimental circuit. Possibilities of the circuit control, mainly by by-pass, were also taken into account. Characteristic curves should serve for preliminery design of the test circuit set up for the turbine model measurements.

Master´s thesis is focused on measurement uncertainty problems, particularly on the uncertainty determination and then on the practical application. The thesis is divided into three parts. A theoretical part deals with issues concerning uncertainties on the theoretical level, transition from the measurement error to the measurement uncertainty. This part also clarifies the basic terms concerning this issues based on “ČSN ISO 5168” standard and another legislative materials. The other part is focused on temperature regulations by thermostatic heads and valves. This part also handles thermostatic heads and valves history, division, generally use and legislative background represented by standard „EN 215“. Practical part follows previous sections. I am determining the measure uncertainty of thermostatic heads and valves flow characteristic determining device. All the results and knowledge obtained are given in the conclusion of the thesis.

Věra Mansfeldová: Time-resolved potentiometry on liquid-liquid interface (Dissertation thesis) Abstract The aim of this work is to explore the method of temporal resolution in potentiometry as a new prospective electrochemical analytical technique. In connection with interface of two immiscible electrolyte solutions (ITIES) it may find utilization in analytical chemistry. This technique up to my knowledge has not been published yet. Potential response of analyte on liquid/liquid interface includes both distribution processes, their temporal resolution and redox processes, which specificity can modified by changing the composition of individual phases. Unlike "classic" potentiometric techniques, limited just to potential determination, this method, which I have given the working name "time resolved potentiometry at liquid-liquid interface" utilizes time development of potential response, which was found to be an analyte-specific function. The time resolved potentiometry presented in this work includes time course of potential response to analytical parameters specific for particular analyte. It brings series of data characterizing the analyte in given environment in a similar manner as spectra and may allow creating analyte-specific data package - fingerprint. Combination with ITIES allows, unlike...

This work is in the introduction focused on the introduction of technologies for electrical energy storage, their description and capturing the main advantages and disadvantages. After this capture follows comparison of the various technologies in terms of efficiency , discharge time and the price for1 kWh . Following section focuses on electrochemical model VRB batteries , which describes the equations describing the behavior of a battery depending on the chemical an electrical properties . In the penultimate chapter, I introduced the language of object- oriented modeling language Modelica and the most common programs based on it, including a short introduction for modeling in MathModelica. The last part deals with the modeling of specific VRB battery, which we have at the faculty.