This research was conducted to provide baseline data on the population dynamics of microcrustacean zooplankton at Prairie Creek Reservoir in Delaware County, Indiana from 24 January 1996 to 30 December 1996. This lake is a shallow lake (average depth <8m) used for recreational fishing and water supply for the local community. No published studies of zooplankton have previously been conducted at this location. I intended to test the hypothesis that Prairie Creek Reservoir has the same microcrustacean populations of shallow lakes in this region. The findings were compared to other studies in several North American lakes and reservoirs. Biotic and abiotic factors were considered as related to the population dynamics of zooplankton. Zooplankton serve as a critical forage base for many fish species, including several of economic importance. Increases and changes in species composition of zooplankton organisms associated with eutrophic conditions may also be an indication of deteriorating water quality (Gannon 1972). The data from this research suggest that Prairie Creek Reservoir has the typical zooplankton populations of a shallow, eutrophic North American lake. The presence of relatively high numbers of organisms generally associated with eutrophic environments, such as Bosmina longirostris, Leptodiaptomus siciloides, Acanthocyclops vernalis, and others supports this idea. The data from this study will be helpful to fisheries and environmental managers and will provide a baseline for future studies. / Department of Biology

The Prairie Creek Reservoir, located in east central Indiana in an agricultural watershed, serves as a secondary drinking water source as well as a recreational facility. Limited research of water quality performed at the reservoir has affected management decisions over the past years, threatening its future.. Consequently, additional investigation was necessary to examine the reservoir dynamics, and continue to provide data to determine long-term water quality trends. Basic water quality parameters were measured using a Hydrolab Sonde, nutrients were measured spectrophotometrically, and a Secchi disk was used to determine water transparency. The Prairie Creek Reservoir was categorized as an eutrophic water body. Concentrations of the measured nutrients were higher in the hypolimnion and increased as summer progressed. Concentration of ammonia and soluble reactive phosphorus exceeded the recommended water quality guidelines. Increased concentration of phosphorus has resulted in nitrogen limitation in the reservoir. Internal loading of nutrients, especially phosphorus, was found to deteriorate water quality. Dissolved oxygen concentrations varied vertically and anoxic conditions frequently reached 50% of the total reservoir depth. Reservoir restoration and watershed protection programs need to address internal P load and nitrogen limitation to prevent development of toxic algal blooms and eutrophication in the future. / Department of Natural Resources and Environmental Management

Ecological restoration techniques are increasingly employed along urban stream corridors. In the past, flood-control projects had negative impacts upon our urban streams and many of these streams suffer from degradation. Cardinal Creek, a stream that flows through Ball State University's campus is one such example. With an increase in urbanization, and subsequent loss of habitat, the stream has become nothing more than an open drain, often carrying bacteria that pose a serious health risk. Following a review of stream restoration principles and "aesthetics of care" principles, the application of these ideas is explored. This project will incorporate, both stream restoration principles and "aesthetic of care" principles in order to produce a potentially ecologically healthy and visually appealing community amenity. / Department of Architecture

Prairie Creek Reservoir in east central Indiana serves as a secondary water supply for the city of Muncie and is also used as a recreational facility. Due to water quality concerns, this study was conducted to characterize spatial and temporal variability of physical and chemical parameters, specifically nutrients, in the sediment interstitial (pore) water at three reservoir locations. The pH, temperature, specific conductivity, ammonia, nitrates, total nitrogen, orthophosphates and total phosphorus, were monitored twice per month from June through September 2006. Spatial variations in the sediment interstitial water were evident for all water quality parameters, except phosphorus and orthophosphates. Variability in temporal nutrient concentrations in pore water was most likely affected by decomposition of organic matter and presence of anoxic conditions in the overlying water. The results showed high internal nutrient loading, especially from phosphorus, in the sediment interstitial water. Under anoxic conditions, these excessive nutrient concentrations within the sediment can be released into the water column and exacerbate eutrophication of the reservoir. This study provides useful assessment of internal nutrient loading in the Prairie Creek Reservoir sediment that should be considered in the implementation of land management strategies and future land development practices in the reservoir watershed. / Department of Natural Resources and Environmental Management

As a result of rapid growth in technology, efforts to remove pollutants from the natural environment have not been able to keep pace with the increasing amount of waste materials and a growing population that further aggravates the situation. This has resulted in the transformation of lakes and ponds into pollution depots. A pilot study for monitoring certain selected trace elements entering and leaving YorkPrairie Creek Pond, situated inside the campus of Ball State University, was undertaken with the view to establishing whether or not this pond has been polluted, like many lakes and ponds, by metal corrosion, engine exhaust gases, and runoff from fertilizers and streets.This investigation involved sampling pond waters at two sites, viz., Influx surface and Effluent surface. Samples were collected once daily at the same time over a total time period of 23 days. Each sample was analyzed, in duplicate, for the heavy metal cations iron, lead, and cadmium, and for the inorganic anions nitrate, orthophosphate, and chloride. Metals were analyzed by atomic absorption spectrophotometry (A. A. S.), while titrimetric and cotorimetric procedures were utilized for the anion analyses.Results have indicated that iron and chloride are the most abundant of all analytes. Precipitation tends to Increase concentrations of analytes in general, possibly due to increased runoff and the lowering of the pH of these waters; leading to increased elemental release into solution. The relative responses of the analyzed ion levels in pond influx and effluent waters have been shown to be similar although their levels were significantly different.Analyte levels were found to be generally higher in effluent than in Influx waters; only chloride was found to depart from this general behavior. This finding led to the conclusion that the selected Ions, with the exception of chloride, tend not to accumulate in the waters of the pond but rather to escape with its effluent. This conclusion is subject to modification by future researchers, to whom the author has recommended a more expanded sampling program that would include the surface and sediment of the pond's interior since the nature of the results (especially for Pb and Cd) would seem to indicate not only that the pond does not exhibit thorough mixing of its constituents (homogeneity) but also the presence of an active remobilization process within its interior.

During this creative project rationale, developed from scientific fact, community needs and the values inherent to the student was utilized as the foundation for decisions associated with the development of a campground facility for Prairie Creek Reservoir. Comprehensive and site specific problem-solving methods were used to form a broad overview of the park and recreation planning and design procedures available to landscape architects.At the comprehensive scale, a survey was conducted to assess the community need for the camping experience. An inventory of existing camping opportunities available to the residents of Delaware County was used, in conjunction with the survey results and recreation standards, in a supply/demand equation to determine the number and the type of camping facilities needed to meet present and future demand for the camping experience. The concept of camping at Prairie Creek Reservoir Park was viewed in this light.Site scale investigations began with the establishment of user and resource criteria. Natural, cultural and economic data were collected from the landscape contained within, and immediately surrounding, the property leased by the Muncie Park Board at the Prairie Creek Reservoir site. This information was analyzed in accordance with the design criteria to produce a series of computer generated maps. The student identified alternative campground sites within the site with the help of these visual aids. The campground site, best meeting the user needs and resource capabilities, was selected and a series of development proposals for the campground facility was prepared. / Department of Landscape Architecture

The overall objective of my thesis research was to develop methodologies for assessing effects of mining effluents on pristine and sensitive northern rivers. I used a multi-trophic level approach in field studies to evaluate current monitoring methods and to determine whether metal mining activities had affected two otherwise pristine rivers that flow into the South Nahanni River, NWT; a World Heritage Site. Upstream reference conditions in the rivers were compared to sites downstream and further downstream of mines. The endpoints evaluated included concentrations of metals in river water, sediments and liver and flesh of slimy sculpin (Cottus cognatus); benthic algal and macroinvertebrate abundance, richness, diversity, and community composition; and various slimy sculpin measures. Elevated concentrations of copper (p=0.002)and iron (p=0.001) in liver tissue of sculpin from the Flat River were associated with high concentrations of mine-derived iron in river water and copper in sediments that were above national guidelines. In addition, sites downstream of the mine on the Flat River had increased algal abundances (p=0.002) and altered benthic macroinvertebrate communities ((p<0.001) whereas the sites downstream of the mine on Prairie Creek had increased benthic macroinvertebrate taxa richness (p=0.050) and improved sculpin condition (males: p=0.008; females: p=0.001). Biological differences in both rivers were consistent with mild enrichment of the rivers downstream of current and historical mining activity. Although the effects of mining activities on riverine biota in these northern rivers are currently limited, results of this research show that there is potential for effects to occur with proposed growth in mining activities.<p> Laboratory exposures were conducted using slimy sculpin, identified as a sentinel fish species in pristine northern rivers, to identify alternative methods for assessing toxicity of contaminants of concern in mining effluents. Ammonia was selected for the exposures based on effluent characteristics of northern mining effluents. Ammonia is known to be an important toxicant in aquatic environments. Although ammonia toxicity has been well studied in many fish species, effects of chronic exposure of slimy sculpin, a critical biomonitoring species for northern aquatic habitats, are not well known. Slimy sculpin were exposed to six concentrations of un-ionized ammonia relevant to concentrations found in northern mining effluents: control (0 ppm), 0.278 ppm, 0.556 ppm, 0.834 ppm, 1.112 ppm, and 1.668 ppm. An LC50 of 1.529 ppm was calculated from mortality data. Histopathological examination of gills indicated significant tissue damage, measured as lamellar fusion and epithelial lifting, at 0.834, 1.112, and 1.668 ppm. Using gill endpoints, NOEC and LOEC were calculated as 0.556 ppm and 0.834 ppm respectively. An EC50 of 0.775 ppm was determined for lamellar fusion and an EC50 of 0.842 ppm for epithelial lifting. Hemorrhage of gills was present in mortalities which occurred at 1.668 ppm of un-ionized ammonia. A significant decrease in liver somatic index (LSI) was seen in both male and female fish at 0.834 and 1.112 ppm, respectively. Gonadosomatic index (GSI) in female fish significantly increased at 1.668 ppm un-ionized ammonia with an associated significant increase in total whole body testosterone concentrations. GSI in male fish also significantly increased at 1.668 ppm but no differences were seen in testosterone concentrations. No significant differences were seen in gonad histopathological assessments or condition factor. Results from this study indicate that ammonia concentrations commonly reported in northern mine effluents hold potential to affect the health of slimy sculpin including acute, chronic, histological and endocrine endpoints. <p> Results from both the field study and laboratory exposures provide direction for future monitoring programs in pristine northern rivers and emphasize the importance of monitoring tools to detect change in these ecosystems. I recommend that monitoring of northern pristine rivers focus on a multi-trophic monitoring approach including indicators in algal and benthic macroinvertebrate communities due to their responsiveness. Laboratory exposures using slimy sculpin should be considered to obtain toxicological information for northern contaminants of concern. Gill histopathology endpoints may be a more sensitive indicator for detecting effects in slimy sculpin exposed to ammonia than traditional chronic endpoints. I also recommend monitoring of metal burdens in periphyton and benthic invertebrates for assessment of exposure to mine effluent and causal association in areas of low fish abundance.

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The overall objective of my thesis research was to develop methodologies for assessing effects of mining effluents on pristine and sensitive northern rivers. I used a multi-trophic level approach in field studies to evaluate current monitoring methods and to determine whether metal mining activities had affected two otherwise pristine rivers that flow into the South Nahanni River, NWT; a World Heritage Site. Upstream reference conditions in the rivers were compared to sites downstream and further downstream of mines. The endpoints evaluated included concentrations of metals in river water, sediments and liver and flesh of slimy sculpin (Cottus cognatus); benthic algal and macroinvertebrate abundance, richness, diversity, and community composition; and various slimy sculpin measures. Elevated concentrations of copper (p=0.002)and iron (p=0.001) in liver tissue of sculpin from the Flat River were associated with high concentrations of mine-derived iron in river water and copper in sediments that were above national guidelines. In addition, sites downstream of the mine on the Flat River had increased algal abundances (p=0.002) and altered benthic macroinvertebrate communities ((p<0.001) whereas the sites downstream of the mine on Prairie Creek had increased benthic macroinvertebrate taxa richness (p=0.050) and improved sculpin condition (males: p=0.008; females: p=0.001). Biological differences in both rivers were consistent with mild enrichment of the rivers downstream of current and historical mining activity. Although the effects of mining activities on riverine biota in these northern rivers are currently limited, results of this research show that there is potential for effects to occur with proposed growth in mining activities.<p> Laboratory exposures were conducted using slimy sculpin, identified as a sentinel fish species in pristine northern rivers, to identify alternative methods for assessing toxicity of contaminants of concern in mining effluents. Ammonia was selected for the exposures based on effluent characteristics of northern mining effluents. Ammonia is known to be an important toxicant in aquatic environments. Although ammonia toxicity has been well studied in many fish species, effects of chronic exposure of slimy sculpin, a critical biomonitoring species for northern aquatic habitats, are not well known. Slimy sculpin were exposed to six concentrations of un-ionized ammonia relevant to concentrations found in northern mining effluents: control (0 ppm), 0.278 ppm, 0.556 ppm, 0.834 ppm, 1.112 ppm, and 1.668 ppm. An LC50 of 1.529 ppm was calculated from mortality data. Histopathological examination of gills indicated significant tissue damage, measured as lamellar fusion and epithelial lifting, at 0.834, 1.112, and 1.668 ppm. Using gill endpoints, NOEC and LOEC were calculated as 0.556 ppm and 0.834 ppm respectively. An EC50 of 0.775 ppm was determined for lamellar fusion and an EC50 of 0.842 ppm for epithelial lifting. Hemorrhage of gills was present in mortalities which occurred at 1.668 ppm of un-ionized ammonia. A significant decrease in liver somatic index (LSI) was seen in both male and female fish at 0.834 and 1.112 ppm, respectively. Gonadosomatic index (GSI) in female fish significantly increased at 1.668 ppm un-ionized ammonia with an associated significant increase in total whole body testosterone concentrations. GSI in male fish also significantly increased at 1.668 ppm but no differences were seen in testosterone concentrations. No significant differences were seen in gonad histopathological assessments or condition factor. Results from this study indicate that ammonia concentrations commonly reported in northern mine effluents hold potential to affect the health of slimy sculpin including acute, chronic, histological and endocrine endpoints. <p> Results from both the field study and laboratory exposures provide direction for future monitoring programs in pristine northern rivers and emphasize the importance of monitoring tools to detect change in these ecosystems. I recommend that monitoring of northern pristine rivers focus on a multi-trophic monitoring approach including indicators in algal and benthic macroinvertebrate communities due to their responsiveness. Laboratory exposures using slimy sculpin should be considered to obtain toxicological information for northern contaminants of concern. Gill histopathology endpoints may be a more sensitive indicator for detecting effects in slimy sculpin exposed to ammonia than traditional chronic endpoints. I also recommend monitoring of metal burdens in periphyton and benthic invertebrates for assessment of exposure to mine effluent and causal association in areas of low fish abundance.